scholarly journals Dynamics of changes in the structural components of the mucosa and cartilage of the larynx of rats at the end of 42 days of experimental opioid exposure and after 56 days with opioid withdrawal

2021 ◽  
Vol 11 (7) ◽  
pp. 201-218
Author(s):  
Khrystyna Ivasivka
Keyword(s):  
Mucous Membrane ◽  
Apical Part ◽  
Structural Components ◽  
Histological Techniques ◽  
Laryngeal Mucosa ◽  
Pas Reaction ◽  
Experimental Group ◽  
Hematoxylin Eosin ◽  
And Cartilage ◽  
Excessive Accumulation

In our work we aimed to study the pathomorphological manifestations among the structural components of the laryngeal mucosa and cartilage in the later stages of the experimental opioid effect. This goal was achieved through the use of histological techniques to visualize the structural components of the laryngeal mucosa and cartilage. Histological sections with a thickness of 5 -7 mm were made. Histological specimens were prepared according to conventional methods using the dye hematoxylin, eosin, azan according to the method of Heidenhain, PAS-reaction according to Mc-Manus and Alcian blue according to Steedman. Microscopic examinations and photographing of the preparations were performed using an MBI-1 microscope and a Nicon D 3100 digital camera.At the end of 42 days of experimental exposure, the epithelial layer of the epiglottis was inhomogeneously thickened, with excessive accumulation on its pharyngeal and apical part of the stratum corneum. Individual epitheliocytes of the pharyngeal part of the epiglottis underwent necrotic changes, the horny substance loosened in places. On the pharyngeal surface of the mucous membrane found a small amount of mucus with impurities of erythrocytes. Dyscirculatory processes were also registered in the animals of the experimental group. In particular, dilatation and overflow of erythrocytes of vessels of own plate of a mucous membrane of an epiglottis, and also polymorphonuclear infiltration of own plate of a mucous membrane were observed.

Dynamics of changes in the structural components of the mucosa and cartilage of the larynx of rats at the end of 28th and 35th days of experimental opioid exposure

2021 ◽  
pp. 79-88
Author(s):  
Khrystyna P. Ivasivka ◽  
Evgeniy V. Paltov ◽  
Zoryana Z. Masna ◽  
Yuri Ya. Kryvko ◽  
Maryana B. Cherkes
Keyword(s):  
Mucous Membrane ◽  
Structural Components ◽  
Fundamental Study ◽  
The Future ◽  
And Cartilage

In our work we aimed to conduct a fundamental study of the process of morphological disorganization of the structural components of the mucous membrane and cartilage of the larynx at the end of the 28 and 35 days of experimental opioid effects at the microstructural level. This information in the future will allow to form a pathomorphological base, which will be used to compare the components of the mucous membrane and cartilage of the larynx in the norm with the dynamics of their changes as a result of experimental opioid effects at different times.

scholarly journals PATHOMORPHOLOGICAL CHARACTERISTICS OF CHANGES IN THE STRUCTURAL COMPONENTS OF THE MUCOSA AND CARTILAGE OF THE LARYNX OF RATS AT THE END OF THE SEVENTH DAY OF THE EXPERIMENTAL OPIOID EFFECT

2021 ◽  
Author(s):  
Ivasivka K. P. ◽  
Paltov E. V. ◽  
Masna Z. Z.
Keyword(s):  
Mucous Membrane ◽  
Structural Components ◽  
Fundamental Study ◽  
The Future ◽  
And Cartilage

In our work we aimed to conduct a fundamental study of the process of morphological disorganization of the structural components of the mucous membrane and cartilage of the larynx at the end of the seventh day of experimental opioid effects at the microstructural level. This information in the future will allow to form a pathomorphological base, which will be used to compare the components of the mucous membrane and cartilage of the larynx in the norm with the dynamics of their changes as a result of experimental opioid effects at different times.

scholarly journals ULTRASTRUCTURAL CHARACTERISTICS OF THE LARYNGEAL MUCOSA AND CARTILAGE RAT IS NORMAL

World Science ◽  
2021 ◽  
Author(s):  
Ivasivka K. P.
Keyword(s):  
Mucous Membrane ◽  
Fundamental Study ◽  
Laryngeal Cartilage ◽  
Laryngeal Mucosa ◽  
The Future ◽  
Ultrastructural Characteristics ◽  
And Cartilage

In our work, the aim was to conduct a fundamental study of the issue concerning the ultrastructural characteristics of the mucous membrane and cartilaginous corset of the rat larynx in the norm.This information in the future will make it possible to form a base that will be used to compare the components of the mucosa and laryngeal cartilage in the norm with the dynamics of their changes as a result of experimental opioid exposure.

scholarly journals CHARACTERISTICS OF THE STRUCTURAL COMPONENTS OF THE MUCOSA AND CARTILAGE OF THE LARYNX OF RATS IN NORMAL

World Science ◽  
2021 ◽  
Author(s):  
Ivasivka Kh. P. ◽  
Paltov E. V. ◽  
Masna Z. Z.
Keyword(s):  
Mucous Membrane ◽  
Structural Components ◽  
Fundamental Study ◽  
Morphological Organization ◽  
The Future ◽  
And Cartilage

The aim was to conduct a fundamental study of the issue concerning the morphological organization of the mucous membrane and cartilaginous corset of the rat larynx in the norm. Our goal is achieved through the use of morphological methods of preparation and microstructural research.This information in the future will allow to form a morphological base, which will be used to compare the components of the mucous membrane and cartilage of the larynx in the norm with the dynamics of their changes as a result of experimental opioid exposure.

scholarly journals Dynamics of changes in the ultrastructural characteristics of the mucosa and cartilage of the larynx of rats at the end of 35 and 42 days of experimental opioid exposure and after 56 days with opioid withdrawal

2021 ◽  
Vol 11 (9) ◽  
pp. 841-851
Author(s):  
K. Ivasivka ◽  
E. Paltov ◽  
Z. Masna ◽  
I. Chelpanova
Keyword(s):  
Mucous Membrane ◽  
Opioid Analgesic ◽  
Ultrastructural Level ◽  
Opioid Withdrawal ◽  
Ultrastructural Organization ◽  
National Medical ◽  
Laryngeal Mucosa ◽  
Medical University ◽  
And Cartilage ◽  
Different Parts

In our work, we studied the ultrastructural organization of rat laryngeal mucosa and cartilage at the end of 35 and 42 days of experimental opioid exposure and at the end of 56 days with opioid withdrawal. As a result of our study at the end of the 35th day at the ultrastructural level revealed hypersecretion of goblet cells, mucus contained impurities of lymphocytes, single neutrophils and erythrocytes. In the enlarged lumens of the hemocapillaries of the lamina propria of the mucous membrane and submucosal base of different parts of the larynx, the adhesion of erythrocytes was noted. At the end of 42 days in rats, the changes were characterized by moderate damage to cytoplasmic organelles in epitheliocytes. Alternative changes developed in the chondrocytes of elastic and hyaline cartilages. Marked by an increase in the number of active fibroblasts, next to which were numerous fibrils of collagen fibers. The main substance of connective tissue, near dilated vessels, was impregnated with transudate, which was accompanied by the development of perivasal edema. Glycogen grains and vacuoles filled with neutral fats were visualized in the cytoplasm of chondrocytes.At the end of 56 days in rats with two-week withdrawal of the opioid analgesic, we found the development of alternative processes in the epitheliocytes of different parts of the larynx, as well as in chondrocytes, especially in elastic cartilage. Dyscirculatory processes in vessels of own plate of a mucous membrane and a submucous basis of various departments of a larynx were registered. Necrotic changes developed in the columnar epitheliocytes of the submucosal mucosa. Mitochondria swelled, their matrix brightened, cristae collapsed. The results of the article correspond to the research plan of  Danylo Halytskyi Lviv National Medical University and are part of the research topic of the Department of Normal Anatomy "Morphofunctional features of organs in pre - and postnatal periods of ontogenesis, under the influence of opioids, food supplements, reconstructive surgery and obesity" 0120U002129) during 2020 - 2024.

scholarly journals ВПЛИВ ФУМОНІЗИНОТОКСИКОЗУ НА МОРФОЛОГІЧНІ ТА БІОХІМІЧНІ ПОКАЗНИКИ КРОВІ ЩУРІВ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 48-52
Author(s):  
Z. Guta
Keyword(s):  
Negative Impact ◽  
The Body ◽  
Phagocytic Index ◽  
Structural Components ◽  
Morphological Indices ◽  
Physiological Values ◽  
Biochemical Indices ◽  
Experimental Group ◽  
Alanine Aminotransferase Activity ◽  
Comprehensive Study

The article deals with the influence of fumonisins on morphological and biochemical indices of rats blood. 20 rats weighing 165-170 g were used in the experiment. It was formed two groups: first group of animals - served as a control, in the second experimental group – chronic fumonisin toxicosis  was reproduced. Rats were daily injected with intra- gastro intestinal 90 mg of fumonisin per one animal. It was established that after the introduction of fumonisin to rats, mycotoxins have a negative impact on the morphological indices of rats blood. It wass found a probable increase in the number of leukocytes to 18.9 g/l, the number of eosinophils to 6.7% segmented neutrophils to 30.7%, and also the decrease in the number of lymphocytes and monocytees to 56.0 and 0.7%. In the analysis of leukocyte formula we have noted the tendency to displacement the nucleus left.Reactions that occur on the background of  the above mentioned toxicity,caused by selective tropism for various tissues of the body; as a result there are nefro-, hepato- and nephrotoxic reactions.To diagnose these changes is possible  after a comprehensive study on the basis of biochemical changes. At research of biochemical parameters in rats blood by the development of chronic fumonisin toxicosis  it was set the reduction of  the level of total protein; indicating the development of violations of proteins exchange in rats organism and protein synthesized liver function.Also it was found that under the conditions of  fumonisin toxicosis  in rats, alanine -aminotransferase activity and aspartate- aminotransferase in their blood throughout the experiment was increased.This is explained by increased permeability of cells under the influence of fumonisin that influenced directly on the membrane, probably, disrupting their structural components. The concentration of creatinine and urea in the blood of experimental rats which were  fed with fumonisin, exceeded the physiological values that were clinical sign of the development of inflammation in rats organism.On the 14th  day the phagocytic activity of neutrophils and phagocytic index of rat blood affected by fumonisin was reduced, indicating the availability of such changes in the immune system of animals.

scholarly journals Morphometric characteristics of the mucous membrane and the cartilaginous component of the larynx are normal at different times during the experimental opioid effect and during withdrawal

Morphologia ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 25-30
Author(s):  
K.P. Ivasivka ◽  
Ye.V. Paltov ◽  
Z.Z. Masna ◽  
I.V. Chelpanova
Keyword(s):  
Mucous Membrane ◽  
Scientific Literature ◽  
Opioid Analgesic ◽  
Morphometric Parameters ◽  
Morphometric Characteristics ◽  
Prolonged Administration ◽  
Laryngeal Mucosa ◽  
Opioid Drugs ◽  
Conventional Methods ◽  
Sexually Mature

Background. The problem of uncontrolled use of opioid drugs is extremely relevant based on the data of domestic and world statistics which are covered in the scientific literature. That is why the study of indicators of morphometric characteristics of the laryngeal mucosa under opioid exposure under experimental opioid exposure will be of interest to both morphologists and practical otorhinolaryngologists. Objective: To study the morphometric parameters of the laryngeal mucosa in normal at different times of the experimental opioid effect and its cancellation. Methods. The material of the study were sexually mature, outbred rats - males in the amount of 61 animals, weighing 80 - 135 g, aged 4.5 - 7.5 months. Histological specimens were prepared according to conventional methods. All morphometric studies were performed using primary (unedited) photographs taken on a Meiji MT4300 LE microscope, Canon EOS 550D x100 lens. All statistical calculations were performed using RStudio v. 1.2.5042. Results. Throughout the experiment, the change of morphometric parameters of the laryngeal mucosa with signs of wavy growth and decline was clearly observed at all times. More positive was the dynamics of morphometric parameters after the abolition of the opioid analgesic, which hypothetically suggests the process of recovery of the mucosa, even after prolonged administration of the opioid.

Histological Techniques for Decalcified Bone and Cartilage

2003 ◽  
pp. 209-220 ◽  
Author(s):  
Qian K. Kang ◽  
Jackie C. LaBreck ◽  
Helen E. Gruber ◽  
Yuehuei H. An
Keyword(s):  
Histological Techniques ◽  
And Cartilage

scholarly journals Morphometry of primary and secondary epidermal laminae in equine hoof

2014 ◽  
Vol 34 (1) ◽  
pp. 79-82
Author(s):  
Luana S. Oliveira ◽  
André R. C. Barreto-Vianna ◽  
André S. Leonardo ◽  
Roberta F. Godoy ◽  
Eduardo M. M. de Lima
Keyword(s):  
Light Microscopy ◽  
Histological Techniques ◽  
Equine Hoof ◽  
Hematoxylin Eosin

We studied the length of primary and secondary epidermal laminae of the toe and the lateral and medial quarters of horses, distributed into proximal, middle and distal thirds of the hooves. Eight limbs from adult crossbred horses, four females and four males, used to pull carts without pedal conditions. Fragments were taken from different regions of the hooves and subjected to conventional histological techniques. The samples were stained with hematoxylin-eosin and analyzed by light microscopy. The primary epidermal laminae were higher in the hooves of forelimbs compared to hindlimbs in the proximal and middle thirds and the regions of the medial quarter and toe. The secondary laminae were higher in forelimb of the middle third and medial quarter. Comparing the length of the epidermal laminae between hoof parts, it was seen that the primary laminae are lower in the proximal third and higher in the toe, while the secondary laminae are lower in the proximal third and medial quarter. The results suggested that the morphology of the laminae in the different regions of the hooves is influenced through the work performed by the animal, as well as through the different distribution of forces.

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