scholarly journals Dynamics of changes in the ultrastructural characteristics of the mucosa and cartilage of the larynx of rats at the end of 35 and 42 days of experimental opioid exposure and after 56 days with opioid withdrawal

2021 ◽  
Vol 11 (9) ◽  
pp. 841-851
Author(s):  
K. Ivasivka ◽  
E. Paltov ◽  
Z. Masna ◽  
I. Chelpanova
Keyword(s):  
Mucous Membrane ◽  
Opioid Analgesic ◽  
Ultrastructural Level ◽  
Opioid Withdrawal ◽  
Ultrastructural Organization ◽  
National Medical ◽  
Laryngeal Mucosa ◽  
Medical University ◽  
And Cartilage ◽  
Different Parts

In our work, we studied the ultrastructural organization of rat laryngeal mucosa and cartilage at the end of 35 and 42 days of experimental opioid exposure and at the end of 56 days with opioid withdrawal. As a result of our study at the end of the 35th day at the ultrastructural level revealed hypersecretion of goblet cells, mucus contained impurities of lymphocytes, single neutrophils and erythrocytes. In the enlarged lumens of the hemocapillaries of the lamina propria of the mucous membrane and submucosal base of different parts of the larynx, the adhesion of erythrocytes was noted. At the end of 42 days in rats, the changes were characterized by moderate damage to cytoplasmic organelles in epitheliocytes. Alternative changes developed in the chondrocytes of elastic and hyaline cartilages. Marked by an increase in the number of active fibroblasts, next to which were numerous fibrils of collagen fibers. The main substance of connective tissue, near dilated vessels, was impregnated with transudate, which was accompanied by the development of perivasal edema. Glycogen grains and vacuoles filled with neutral fats were visualized in the cytoplasm of chondrocytes.At the end of 56 days in rats with two-week withdrawal of the opioid analgesic, we found the development of alternative processes in the epitheliocytes of different parts of the larynx, as well as in chondrocytes, especially in elastic cartilage. Dyscirculatory processes in vessels of own plate of a mucous membrane and a submucous basis of various departments of a larynx were registered. Necrotic changes developed in the columnar epitheliocytes of the submucosal mucosa. Mitochondria swelled, their matrix brightened, cristae collapsed. The results of the article correspond to the research plan of  Danylo Halytskyi Lviv National Medical University and are part of the research topic of the Department of Normal Anatomy "Morphofunctional features of organs in pre - and postnatal periods of ontogenesis, under the influence of opioids, food supplements, reconstructive surgery and obesity" 0120U002129) during 2020 - 2024.

scholarly journals Morphometric characteristics of the mucous membrane and the cartilaginous component of the larynx are normal at different times during the experimental opioid effect and during withdrawal

Morphologia ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 25-30
Author(s):  
K.P. Ivasivka ◽  
Ye.V. Paltov ◽  
Z.Z. Masna ◽  
I.V. Chelpanova
Keyword(s):  
Mucous Membrane ◽  
Scientific Literature ◽  
Opioid Analgesic ◽  
Morphometric Parameters ◽  
Morphometric Characteristics ◽  
Prolonged Administration ◽  
Laryngeal Mucosa ◽  
Opioid Drugs ◽  
Conventional Methods ◽  
Sexually Mature

Background. The problem of uncontrolled use of opioid drugs is extremely relevant based on the data of domestic and world statistics which are covered in the scientific literature. That is why the study of indicators of morphometric characteristics of the laryngeal mucosa under opioid exposure under experimental opioid exposure will be of interest to both morphologists and practical otorhinolaryngologists. Objective: To study the morphometric parameters of the laryngeal mucosa in normal at different times of the experimental opioid effect and its cancellation. Methods. The material of the study were sexually mature, outbred rats - males in the amount of 61 animals, weighing 80 - 135 g, aged 4.5 - 7.5 months. Histological specimens were prepared according to conventional methods. All morphometric studies were performed using primary (unedited) photographs taken on a Meiji MT4300 LE microscope, Canon EOS 550D x100 lens. All statistical calculations were performed using RStudio v. 1.2.5042. Results. Throughout the experiment, the change of morphometric parameters of the laryngeal mucosa with signs of wavy growth and decline was clearly observed at all times. More positive was the dynamics of morphometric parameters after the abolition of the opioid analgesic, which hypothetically suggests the process of recovery of the mucosa, even after prolonged administration of the opioid.

scholarly journals ULTRASTRUCTURAL CHARACTERISTICS OF THE LARYNGEAL MUCOSA AND CARTILAGE RAT IS NORMAL

World Science ◽  
2021 ◽  
Author(s):  
Ivasivka K. P.
Keyword(s):  
Mucous Membrane ◽  
Fundamental Study ◽  
Laryngeal Cartilage ◽  
Laryngeal Mucosa ◽  
The Future ◽  
Ultrastructural Characteristics ◽  
And Cartilage

In our work, the aim was to conduct a fundamental study of the issue concerning the ultrastructural characteristics of the mucous membrane and cartilaginous corset of the rat larynx in the norm.This information in the future will make it possible to form a base that will be used to compare the components of the mucosa and laryngeal cartilage in the norm with the dynamics of their changes as a result of experimental opioid exposure.

scholarly journals Dynamics of changes in the structural components of the mucosa and cartilage of the larynx of rats at the end of 42 days of experimental opioid exposure and after 56 days with opioid withdrawal

2021 ◽  
Vol 11 (7) ◽  
pp. 201-218
Author(s):  
Khrystyna Ivasivka
Keyword(s):  
Mucous Membrane ◽  
Apical Part ◽  
Structural Components ◽  
Histological Techniques ◽  
Laryngeal Mucosa ◽  
Pas Reaction ◽  
Experimental Group ◽  
Hematoxylin Eosin ◽  
And Cartilage ◽  
Excessive Accumulation

In our work we aimed to study the pathomorphological manifestations among the structural components of the laryngeal mucosa and cartilage in the later stages of the experimental opioid effect. This goal was achieved through the use of histological techniques to visualize the structural components of the laryngeal mucosa and cartilage. Histological sections with a thickness of 5 -7 mm were made. Histological specimens were prepared according to conventional methods using the dye hematoxylin, eosin, azan according to the method of Heidenhain, PAS-reaction according to Mc-Manus and Alcian blue according to Steedman. Microscopic examinations and photographing of the preparations were performed using an MBI-1 microscope and a Nicon D 3100 digital camera.At the end of 42 days of experimental exposure, the epithelial layer of the epiglottis was inhomogeneously thickened, with excessive accumulation on its pharyngeal and apical part of the stratum corneum. Individual epitheliocytes of the pharyngeal part of the epiglottis underwent necrotic changes, the horny substance loosened in places. On the pharyngeal surface of the mucous membrane found a small amount of mucus with impurities of erythrocytes. Dyscirculatory processes were also registered in the animals of the experimental group. In particular, dilatation and overflow of erythrocytes of vessels of own plate of a mucous membrane of an epiglottis, and also polymorphonuclear infiltration of own plate of a mucous membrane were observed.

scholarly journals Ultrastructural characteristics of the rats laryngeal mucosa and cartilage on the 7 and 14 days of the experimental opioid effect

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 89-95
Author(s):  
K.P. Ivasivka ◽  
Ye.V. Paltov ◽  
Z.Z. Masna ◽  
I.V. Chelpanova ◽  
O.V. Dudok
Keyword(s):  
Lamina Propria ◽  
Vascular Wall ◽  
Male Rats ◽  
Control Group ◽  
Ultrastructural Organization ◽  
Main Task ◽  
Laryngeal Mucosa ◽  
Group 2 ◽  
Animal Care ◽  
And Cartilage

Background and objective. Our main task was to study the ultrastructural organization of the laryngeal mucosa and cartilage on the seventh and fourteenth day of opioid exposure. Methods. The material for the study were mature, outbred male rats in the number of 32 animals, weighing 80g, aged 4.5 months. Animals were injected intramuscularly with Nalbuphine once a day at the same time (10-11 hours in the morning) for 14 days. The initial dose of Nalbuphine was 8 mg / kg during the first week, 15 mg / kg during the second week of the experimental study. Thus, we created the conditions for chronic opioid exposure. Animals were divided into 3 experimental groups: 1 – control group; 2 - animals that received the drug for 7 days; 3 - animals that received the drug for 14 days). All animals were kept in a vivarium. Animal care, labeling and all other manipulations were carried out taking into account the issues of bioethical norms. Before collecting necropsy material, the animal was removed from the experiment with diethyl ether. The the larynx mucosa and rats cartilage were used as the material for ultrastructural examination. Ultrastructural capsule was prepared according to conventional methods. Results. As a result of experimental opioid exposure at the end of 7 days we found alternative changes in the epithelial cells of different parts of the larynx, dyscirculatory changes in blood vessels of lamina propria and submucosa, as well as the destruction of chondrocytes of hyaline and elastic cartilages. The hemocapillaries of lamina propria and submucosa, especially in the ventricles of the larynx and in the subchondral region, were dilated, overflowing with erythrocytes. In the laryngeal mucosa and submucosal base of the laryngeal ventricles, in addition to the overflow of hemocapillaries with erythrocytes, a moderate accumulation of perivascular transudate was noted. At the end of the 14th day of the experimental opioid effect, a pronounced hemocapillaries injury was found, which was accompanied by an increase in the permeability of the vascular wall. In addition to blood plasma, erythrocytes were visualized in the enlarged lumens of hemocapillaries. The development of degenerative changes of endothelial cells, which were accompanied by inhomogeneous dilation of the tubules of the smooth and granular endoplasmic reticulum, was noted. The main substance of the connective tissue of the mucous membrane and submucosal base, especially around the vessels was impregnated with transudate.

scholarly journals ЕЛЕКТРОННОМІКРОСКОПІЧНІ ДОСЛІДЖЕННЯ ПАРОДОНТА ПРИ ДІЇ ОПІОЇДУ ВПРОДОВЖ ШЕСТИ ТИЖНІВ

2019 ◽  
Vol 1 (8(38)) ◽  
pp. 18-22
Author(s):  
Фік В. Б. ◽  
Савка І. І. ◽  
Пальтов Є. В. ◽  
Кривко Ю. Я.
Keyword(s):  
Mucous Membrane ◽  
Opioid Analgesic ◽  
Ultrastructural Level ◽  
Structural Components ◽  
Microcirculatory Bed ◽  
Periodontal Tissues ◽  
Morphological Basis ◽  
Experimental Effect ◽  
Cellular Components

The following work is aimed at studying the features of the restructuring of the structural components of periodontal tissues under the conditions of a six-week experimental effect of an opioid analgesic at the ultrastructural level. Destructive changes were found in all cellular components of the mucous membrane of the gums, the periodontium and in the links of the microcirculatory bed. The ultrastructural manifestations in periodontal tissues that occur with the action of an opioid analgesic for six weeks may serve as a morphological basis for further study of the dynamics of submicroscopic changes in the later stages of opioid exposure in the experiment.

Neurorehabilitation in the Early Recovery Period of Ischemic Stroke. Pharmacology Support

2021 ◽  
Vol 63 (1) ◽  
pp. 22-25
Author(s):  
Denys N. Khramtsov ◽  
Olexandr N. Stoyanov ◽  
Tetiana N. Muratova ◽  
Olexandr R. Pulyk
Keyword(s):  
Ischemic Stroke ◽  
Acute Stroke ◽  
Recovery Period ◽  
Rehabilitation Medicine ◽  
Neuroprotective Agents ◽  
Early Recovery ◽  
National Medical ◽  
Cholinergic Agents ◽  
Early Recovery Period ◽  
Medical University

Aim: The aim of the study was to evaluate the clinical outcome in the use of neuroprotective agents in the acute period of ischemic stroke. Material and Methods: The study was performed on the basis of the stroke of the Center for Reconstructive and Rehabilitation Medicine (University Clinic) of the Odessa National Medical University. A retrospective analysis of clinical outcomes of 115 patients with acute stroke was conducted. Results: An average NIHSS score at discharge was 4.1±0.1 points when treated with no refinery, then it reached 3.6±0.1 points when using peptidergic drugs, and 3.4±0.1 when using D-fdf. 3.1±0.1 points. When using D-FDF, the MMSE score was 3.5±0.1 points, whereas when using cholinergic agents, this index did not exceed 26.9±1.5 points, and when using peptidergic agents - 26.8±1.4 points. Conclusion: The use of neuroprotective agents positively affects the effectiveness of neuro-rehabilitation in patients with acute stroke. The best results in three months after the hospitalization were obtained for peptidergic agents and D-fructose-1,6-diphosphate.

scholarly journals The human sacrum shape: metamorphoses and relation of its parts

2018 ◽  
Vol 22 (1) ◽  
pp. 14-18
Author(s):  
V.G. Cherkasov ◽  
O.V. Malikov
Keyword(s):  
Human Body ◽  
Sacroiliac Joint ◽  
Vertebral Column ◽  
Human Life ◽  
Anatomical Study ◽  
External Factors ◽  
Multiple Factors ◽  
National Medical ◽  
University Department ◽  
Medical University

The human sacrum shape and its metamorphoses should be considered due to their effect, primarily mechanical, predisposing for the vertebral column function evolution. The aim of the study is to define the human sacrum shapes and explain for such variability. The object of the study is represented with 68 sacrums obtained from the review collection of anatomical study preparations of the Bogomolets National Medical University Department of Anatomy. Each of the known human sacrum shapes represents the stage of the process of “sinking” of the sacral vertebrae bodies into the sacrum, with simultaneous fixation of external apices of their lateral parts in the sacroiliac joint. Generally, metamorphoses of the sacrum shape make up an accommodation for the new mechanical conditions as well as causal external factors. The sacrum continuously sustains multiple factors’ effects in each moment of the human life, which affects its shape and metamorphoses. The analysis of the human sacrum shape and functions reveals important instances: first, certain parts of the sacrum don’t preserve permanent relations; secondly, alterations in these relations keep to a certain sequence. This issue permits us to consider various sacrum shapes as sequential stages of the same process, occurring in the human body.

scholarly journals System of Educational Process Organization at the Department of Endocrinology and the Department of Internal Medicine No 1, Immunopathology and Allergology named after academician Neiko Ye.M. of Ivano-Frankivsk National Medical University

2018 ◽  
Vol 25 (1) ◽  
Author(s):  
Oksana Didushko ◽  
Petro Herych ◽  
Iryna Cherniavska
Keyword(s):  
Internal Medicine ◽  
Medical School ◽  
Educational Process ◽  
National Medical ◽  
The Subject ◽  
Medical University ◽  
Long Term Experience ◽  
Module System

The The article highlights the system of educational process organization during module 1 of the subject “Internal Medicine” at the Department of Endocrinology and the Department of Internal Medicine No 1, Immunopathology and Allergology named after academician Neiko Ye.M. of Ivano-Frankivsk National Medical University. The possibilities of combining long-term experience and the traditions of national medical school with the principles of the credit-module system are described.

Sign in / Sign up

Export Citation Format

Share Document