scholarly journals Effects of Chlorpromazine, Pentoxifylline and Dexamethasone on mRNA Expression of Lipopolysaccharide-Induced Inflammatory Cytokines in Bovine Peripheral Blood Mononuclear Cells

2002 ◽  
Vol 64 (8) ◽  
pp. 723-726 ◽  
Author(s):  
Yoshio KIKU ◽  
Hironori MATSUZAWA ◽  
Hiromichi OHTSUKA ◽  
Nobuhiro TERASAKI ◽  
Shigeo FUKUDA ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

EFFECTS OF TARAXACUM MONGOLICUM EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED NITRIC OXIDE AND CYTOKINES PRODUCTION BY BOVINE PERIPHERAL BLOOD MONONUCLEAR CELLS

2016 ◽  
Vol 42 (04) ◽  
pp. 203-212
Author(s):  
Kuo-Hua Lee ◽  
Kai-Chen Hsu ◽  
Yu-Shan Wang ◽  
Chia-Chou Yeh ◽  
Jih-Yih Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Bovine Mastitis ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Pro Inflammatory Cytokines

The objective of this study is to investigate Taraxacum mongolicum (TM) as a therapeutic alternative for preventing and treating bovine mastitis. The effect of the anti-inflammatory activity of Taraxacum mongolicum extract (TME) on lipopolysaccharide (LPS)-induced responses was studied in the bovine peripheral blood mononuclear cells (PBMCs). The dried plant TM was extracted with 10 volumes of distilled water to generate its water extract. PBMCs were pretreated with various concentrations of TME (0, 1, 10, 100, 1000 [Formula: see text]g/mL) and subsequently incubated with LPS (1 [Formula: see text]g/mL). Cell viability was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylthiazolium bromide (MTT) assay. The level of nitric oxide (NO) was determined by using Griess reagent assay. The mRNA expression levels of pro-inflammatory cytokines including interleukin (IL)-1[Formula: see text], IL-6, IL-8, tumor necrosis factor (TNF)-[Formula: see text] and granulocyte chemotactic protein (GCP)-2 were determined by using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results showed no significant cytotoxic effects on the PBMCs at various treated concentrations of TME. Treatment of TME (100 and 1000 [Formula: see text]g/mL) significantly inhibited NO production in LPS-stimulated PBMCs. TME (10 [Formula: see text]g/mL) significantly inhibited LPS-stimulated IL-1[Formula: see text], IL-6, IL-8, TNF-[Formula: see text] and GCP-2 mRNA expression in PBMCs at a time-dependent manner. In this article, we reported for the first time that TME significantly inhibited production of NO and pro-inflammatory cytokines in LPS-stimulated PBMCs. This finding could be useful for clinical practice in preventing and treating bovine mastitis.

Sensitivity and reliability of zinc transporter and metallothionein gene expression in peripheral blood mononuclear cells as indicators of zinc status: responses to ex vivo zinc exposure and habitual zinc intake in humans

2020 ◽  
pp. 1-8
Author(s):  
Stephen R. Hennigar ◽  
Alyssa M. Kelley ◽  
Bradley J. Anderson ◽  
Nicholes J. Armstrong ◽  
Holly L. McClung ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Human Subjects ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Essential Nutrient ◽  
Zn Status

Abstract Zn is an essential nutrient for humans; however, a sensitive biomarker to assess Zn status has not been identified. The objective of this study was to determine the reliability and sensitivity of Zn transporter and metallothionein (MT) genes in peripheral blood mononuclear cells (PBMCs) to Zn exposure ex vivo and to habitual Zn intake in human subjects. In study 1, human PBMCs were cultured for 24 h with 0–50 µm ZnSO4 with or without 5 µm N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), and mRNA expression of SLC30A1-10, SLC39A1-14, MT1 subtypes (A, B, E, F, G, H, L, M and X), MT2A, MT3 and MT4 mRNA was determined. In study 2, fifty-four healthy male and female volunteers (31·9 (sd 13·8) years, BMI 25·7 (sd 2·9) kg/m2) completed a FFQ, blood was collected, PBMCs were isolated and mRNA expression of selected Zn transporters and MT isoforms was determined. Study 1: MT1E, MT1F, MT1G, MT1H, MT1L, MT1M, MT1X, MT2A and SLC30A1 increased with increasing concentrations of Zn and declined with the addition of TPEN. Study 2: Average daily Zn intake was 16·0 (sd 5·3) mg/d (range: 9–31 mg/d), and plasma Zn concentrations were 15·5 (SD 2·8) μmol/l (range 11–23 μmol/l). PBMC MT2A was positively correlated with dietary Zn intake (r 0·306, P = 0·03) and total Zn intake (r 0·382, P < 0·01), whereas plasma Zn was not (P > 0·05 for both). Findings suggest that MT2A mRNA in PBMCs reflects dietary Zn intake in healthy adults and may be a component in determining Zn status.

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