scholarly journals Latency and Persistence of Bovine Herpesvirus Type 4, Strain B11-41, in Bovine Nervous Tissues

2003 ◽  
Vol 65 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Akihiro ASANO ◽  
Yasuo INOSHIMA ◽  
Kenji MURAKAMI ◽  
Yuko IKETANI ◽  
Yasunori YAMAMOTO ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Herpesvirus Type

Genomic analysis of bovine herpesvirus type 4 (BoHV-4) from Argentina: High genetic variability and novel phylogenetic groups

2012 ◽  
Vol 160 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
A.E. Verna ◽  
J.M. Manrique ◽  
S.E. Pérez ◽  
M.R. Leunda ◽  
S.B. Pereyra ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Genomic Analysis ◽  
Bovine Herpesvirus ◽  
Phylogenetic Groups ◽  
High Genetic Variability ◽  
Herpesvirus Type

scholarly journals Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

2017 ◽  
Vol 130 ◽  
pp. 21-27
Author(s):  
Bianca S. Siedler ◽  
Bárbara C. Roloff ◽  
Gizele L. de Sá ◽  
Alessandra Neis ◽  
Fabrício R. Conceição ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Pichia Pastoris ◽  
Bovine Herpesvirus ◽  
Infected Cattle ◽  
Secretory Expression ◽  
Glycoprotein E ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 1

scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

scholarly journals Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1

2011 ◽  
Vol 41 (2) ◽  
pp. 307-313
Author(s):  
Maria do Carmo Cilento ◽  
Edviges Maristela Pituco ◽  
Ricardo Spacagna Jordão ◽  
Cláudia Pestana Ribeiro ◽  
Moacir Marchiori Filho ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Neutralization Assay ◽  
Elisa Test ◽  
Antibody Responses ◽  
Inactivated Vaccine ◽  
Serum Samples ◽  
Post Vaccination ◽  
Herpesvirus Type ◽  
Virus Neutralization Assay ◽  
Igg2 Antibody

An experimental inactivated vaccine against bovine herpesvirus-1 (BoHV-1) was produced aiming to evaluate the systemic and local antibody responses in 12 seronegative heifers, after vaccination and revaccination. Serum samples were submitted to virus neutralization assay and to ELISA test for detection of IgG1 and IgG2 isotypes. Nasal secretion samples were submitted to the same ELISA test for detection of IgG1 and IgG2 isotypes. The results showed that moderate to high neutralizing titres and IgG1 and IgG2 antibody responses were induced after the second vaccination in the serum and in nasal secretions up to 114 days post vaccination. IgG2 antibodies were the prevalent isotype for most of the post-vaccination period. The results indicate that BoHV-1 experimental inactivated vaccine elicited potentially protective IgG1 and IgG2 antibody levels, both in the systemic and mucosal compartments.

scholarly journals Experimental infection of rabbits with a recombinant bovine herpesvirus type 5 (BoHV-5) gI, gE and US9-negative

2009 ◽  
Vol 29 (11) ◽  
pp. 913-918 ◽  
Author(s):  
Alessandra D'Avila Silva ◽  
Ana Cláudia Franco ◽  
Paulo Augusto Esteves ◽  
Fernando Rosado Spilki ◽  
Paulo Michel Roehe
Keyword(s):  
Neurological Disease ◽  
Age Groups ◽  
Clinical Signs ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Herpesvirus Type ◽  
Viral Genes ◽  
Bovine Herpesvirus Type 5 ◽  
Two Age Groups

Bovine herpesvirus type 5 (BoHV-5) is a major cause of viral meningoencephalitis in cattle. The expression of different viral proteins has been associated with BoHV-5 neuropathogenesis. Among these, gI, gE and US9 have been considered essential for the production of neurological disease in infected animals. To evaluate the role of gI, gE and US9 in neurovirulence, a recombinant from which the respective genes were deleted (BoHV-5 gI-/gE-/US9-) was constructed and inoculated in rabbits of two age groups (four and eight weeks-old). When the recombinant virus was inoculated through the paranasal sinuses of four weeks-old rabbits, neurological disease was observed and death was the outcome in 4 out of 13 (30.7 %) animals, whereas clinical signs and death were observed in 11/13 (84.6%) of rabbits infected with the parental virus. In eight weeks-old rabbits, the BoHV-5 gI-/gE-/US9- did not induce clinically apparent disease and could not be reactivated after dexamethasone administration, whereas wild type BoHV-5 caused disease in 55.5% of the animals and was reactivated. These findings reveal that the simultaneous deletion of gI, gE and US9 genes did reduce but did not completely abolish the neurovirulence of BoHV-5 in rabbits, indicating that other viral genes may also play a role in the induction of neurological disease.

The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport

2011 ◽  
Vol 152 (3-4) ◽  
pp. 270-279 ◽  
Author(s):  
S.I. Chowdhury ◽  
M.C.S. Brum ◽  
C. Coats ◽  
A. Doster ◽  
Huiyong Wei ◽  
...  
Keyword(s):  
Axonal Transport ◽  
Envelope Protein ◽  
Bovine Herpesvirus ◽  
Herpesvirus Type ◽  
Acidic Domain ◽  
Anterograde Axonal Transport ◽  
Bovine Herpesvirus Type 1

scholarly journals Characterization of Monoclonal Antibodies against Bovine herpesvirus type 1 selected by Phage Display Technology

2017 ◽  
Vol 38 (6) ◽  
pp. 3915
Author(s):  
Greice Japolla ◽  
Ana Flávia Batista Penido ◽  
Greyciele Rodrigues Almeida ◽  
Luiz Artur Mendes Bataus ◽  
Jair Pereira Cunha Junior ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Bovine Herpesvirus ◽  
Single Chain ◽  
Phage Display Technology ◽  
Herpesvirus Type ◽  
Wide Range ◽  
Display Technology ◽  
Bovine Herpesvirus Type 1

The specificity of monoclonal antibodies (mAbs) to desired targets makes these molecules suitable for therapeutic and diagnostic uses against a wide range of pathogens. Phage display antibody libraries offer one method by which mAbs can be selected for, without the use of conventional hybridoma technology. In this work, phage display technology was used to construct, select and characterize a combinatorial single chain fragment variable (scFv) antibody library against bovine herpesvirus type 1 (BoHV-1) from the immune repertoire of chickens immunized with the virus. In silico analysis of the hypervariable domains of the antibody heavy chains revealed a high frequency of scFv fragments with low variability, suggesting that selection had probably been carried out and favored by a few im-munogenic viral antigens. The reactivity of the scFv fragments selected against BoHV-1 was demon-strated by Phage-ELISA. A significant increase in antibody reactivity to the target was observed after six rounds of library selection, showing its potential use as a molecule for BoHV-1 diagnosis. The strategy described here opens up a field for the use of phage display as a tool for selection of mono-clonal antibodies that could be used for theranostic applications against infectious and parasitic dis-eases of veterinary interest.

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