scholarly journals Quantitative Analysis of Staphylococcus aureus in Skimmed Milk Powder by Real-Time PCR

2005 ◽  
Vol 67 (10) ◽  
pp. 1037-1041 ◽  
Author(s):  
Tetsuya IKEDA ◽  
Naoto TAMATE ◽  
Keiji YAMAGUCHI ◽  
Sou-ichi MAKINO
Keyword(s):  
Staphylococcus Aureus ◽  
Quantitative Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Milk Powder ◽  
Skimmed Milk ◽  
Skimmed Milk Powder

scholarly journals Propidium monoazide combined with real-time PCR for selective detection of viable Staphylococcus aureus in milk powder and meat products

2015 ◽  
Vol 98 (3) ◽  
pp. 1625-1633 ◽  
Author(s):  
Zhihong Zhang ◽  
Wenting Liu ◽  
Hengyi Xu ◽  
Zoraida P. Aguilar ◽  
Nagendra P. Shah ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Real Time Pcr ◽  
Milk Powder ◽  
Meat Products ◽  
Selective Detection ◽  
Propidium Monoazide

scholarly journals Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sanaz Dehbashi ◽  
Hamed Tahmasebi ◽  
Behrouz Zeyni ◽  
Mohammad Reza Arabestani
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Gene Expressions ◽  
Resistance Development ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems ◽  
Lactamase Gene

Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA)-bloodstream infections (BSI) are predominantly seen in the hospital or healthcare-associated host. Nevertheless, the interactions of virulence factor (VFs) regulators and β-lactam resistance in MRSA-BSI are unclear. This study aims to characterize the molecular relationship of two-component systems of VFs and the expression of the β-lactamase gene in MRSA-BSI isolates. In this study, 639 samples were collected from BSI and identified by phenotypic methods. We performed extensive molecular characterization, including SCCmec type, agr type, VFs gene profiles determinations, and MLST on isolates. Also, a quantitative real-time PCR (q-RT PCR) assay was developed for identifying the gene expressions. Results Ninety-one (91) S. aureus and 61 MRSA (67.0%) strains were detected in BSI samples. The presence of VFs and SCCmec genes in MRSA isolates were as follows: tst (31.4%), etA (18.0%), etB (8.19%), lukS-PVL (31.4%), lukF-PV (18.0%), lukE-lukD (16.3%), edin (3.2%), hla (16.3%), hlb (18.0%), hld (14.7%), hlg (22.9%), SCCmecI (16.3%), SCCmecII (22.9%), SCCmecIII (36.0%), SCCmecIV (21.3%), and SCCmecV (16.3%). Quantitative real-time PCR showed overexpression of mecRI and mecI in the toxigenic isolates. Moreover, RNAIII and sarA genes were the highest expressions of MRSA strains. The multi-locus sequence typing data confirmed a high prevalence of CC5, CC8, and CC30. However, ST30, ST22, and ST5 were the most prevalent in the resistant and toxigenic strains. Conclusion We demonstrated that although regulation of β-lactamase gene expressions is a significant contributor to resistance development, two-component systems also influence antibiotic resistance development in MRSA-BSI isolates. This indicates that resistant strains might have pathogenic potential. We also confirmed that some MLST types are more successful colonizers with a potential for MRSA-BSI.

Price and volatility transmission in, and between, skimmed milk powder, livestock feed and oil markets

2017 ◽  
Vol 46 (4) ◽  
pp. 248-257 ◽  
Author(s):  
Dennis Bergmann ◽  
Declan O’Connor ◽  
Andreas Thümmel
Keyword(s):  
Crude Oil ◽  
Milk Powder ◽  
Multivariate Garch ◽  
Volatility Transmission ◽  
Crude Oil Prices ◽  
Multivariate Garch Model ◽  
Oil Markets ◽  
Skimmed Milk ◽  
Derivative Markets ◽  
Skimmed Milk Powder

Price and volatility transmission effects between European Union (EU) and World skimmed milk powder (SMP) prices, as well as those between both SMP series, soybeans and crude oil prices from 2004 to 2014 were analysed using a vector error correction model combined with a multivariate GARCH model. The results show significant transmission effects between EU and World SMP prices, but no significant transmission effects from soybeans or crude oil to either of the SMP prices. For policymakers and modellers, these results indicate the need to consider World SMP prices when considering EU prices. On the other hand, the finding of no transmission effects from soybean to SMP prices reduces the opportunity for a successful cross-hedging for dairy commodities using well-established soybean derivative markets.

Multicenter validation study of two blockcycler- and one capillary-based real-time PCR methods for the detection of Salmonella in milk powder

2007 ◽  
Vol 117 (2) ◽  
pp. 211-218 ◽  
Author(s):  
Burkhard Malorny ◽  
Dietrich Mäde ◽  
Paul Teufel ◽  
Cornelia Berghof-Jäger ◽  
Ingrid Huber ◽  
...  
Keyword(s):  
Real Time ◽  
Validation Study ◽  
Real Time Pcr ◽  
Milk Powder ◽  
Multicenter Validation

scholarly journals Feasibility of using jack fruit seeds powder as a substitute of skimmed milk powder for making traditional yogurt

2020 ◽  
pp. 1
Author(s):  
Shabbir Ahmed ◽  
Most Khairunnesa ◽  
Mst Habiba ◽  
Md Islam ◽  
S Hoque ◽  
...  
Keyword(s):  
Milk Powder ◽  
Skimmed Milk ◽  
Skimmed Milk Powder

Effect of Storage on Physio-Chemical Properties of Peanut Yogurt

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Naukhaiz Abbas ◽  
Zainab Sharmeen ◽  
Shahid Bashir ◽  
Misbah Arshad ◽  
Zargham Mazhar
Keyword(s):  
Chemical Analysis ◽  
Milk Powder ◽  
Chemical Properties ◽  
Chemical Parameters ◽  
Total Solids ◽  
Storage Treatment ◽  
Skimmed Milk ◽  
Skimmed Milk Powder

Peanuts may be consumed in a variety of processed forms like roasted, raw and processed etc. andrepresent as a multimillion dollar crop worldwide with many potential dietary benefits as it contains highprotein and health effective oils. Objective: The present investigation was planned to evaluate thephysio-chemical properties of peanut milk yogurt by the addition of different concentration of peanut milk(0 %, 10 %, 20 % and 30 %), skimmed milk liquid (60 %, 70 %, 80 %, and 90 %), skimmed milk powder (9 %)and sugar (1 %). Methods: The physio-chemical tests (pH, acidity, moisture, ash, fat, protein, syneresis,and viscosity) were examined after every 5 days of interval for a period of 15 days at 4 ºC. Results: Theresults of physio-chemical analysis revealed that pH, ash, fat, protein and viscosity decrease duringstorage period where as acidity, moisture and rate of syneresis increased during storage. Treatment T1(10 % peanut milk) was comparatively best for manufacturing of peanut milk yogurt followed by T2 (20 %peanut milk + 70 % skimmed milk liquid + 9 % skimmed milk powder + 1 % sugar) while peanut milk yogurtfrom (30 % peanut milk + 60 % skimmed milk liquid + 9 % skimmed milk powder + 1 % sugar) had the lowestdegree of firmness. Conclusions: It was noticed that correlation among fat, total solids and proteincontents in peanut milk affect the extent of serum separation and pH of yogurt. The storage hadsignificant effects on all physio-chemical parameters. Treatments had significant effect on all physiochemicalparameters.

Sign in / Sign up

Export Citation Format

Share Document