scholarly journals In vivo anti-hypercholesterolemic effect of buttermilk, milk fat globule membrane and Enterococcus faecium FFNL-12

2019 ◽  
Vol 7 (2) ◽  
pp. 517-531 ◽  
Author(s):  
Khaled ElSaadany ◽  
Heba-tallah Abd-Elhaleem
Keyword(s):  
Antioxidant Activity ◽  
Lipid Profile ◽  
Enterococcus Faecium ◽  
Milk Fat ◽  
Serum Lipid ◽  
Standard Diet ◽  
Milk Fat Globule Membrane ◽  
Hypercholesterolemic Diet ◽  
Milk Fat Globule ◽  
Fat Globule

The present study was undertaken to evaluate the hypocholesterolemic activity of buttermilk, milk fat globule membrane (MFGM) and Enterococcus faecium FFNL-12 in rat model. Thirty-sixth male Abino rats were divided into six groups. The first one (coded as G1) was fed a standard diet containing 10% corn oil as fat source while remaining five (coded G2 to G6) were fed hypercholesterolemic diets in which oil was replaced with animal grease. Animals subjected to treatment G1 served as healthy control while those in G2 were assigned as hypocholesterolemic animals which did not receive any treatment. The remaining experimental groups were designed to assess the hypocholesterolemic effect of intragastric adminstartion of dose of 109 CFU/Kg body weight of Enterococcus faecium FFNL-12 (G3), Enterococcus faecium FFNL-12/butter milk (G4), buttermilk (G5) and milk fat globule membrane (MFGM). After four weeks, animals were evaluated in relation to growth, fecal pH, organs weight, serum lipid profile, antioxidant activity of liver tissue, liver and heart function and liver histopathological architecture. Results revealed that animals fed hypercholesterolemic diet (G2-G6) had significantly lower faecal pH and liver weight compared with those fed standard diet (G1). Treatments applied to animals fed hypercholesterolemic diet with the above mentioned additions (G3 to G6) appeared to improve both cardiac and hepatic functions, serum lipid profile and glucose concentration and liver histopathological architecture compared with animals subjected to G2 treatment. In most cases, treatment with MFGM appeared to be the most effective to avoid adverse effects associated to feeding hypercholesterolemic diet. MFGM fraction as well as E. faecium FFNL-12/buttermilk combination were effective in reducing serum lipids and glucose levels to the normal range. This combination also had potential antioxidant activity and ability to improve liver and heart functions.

A positive impact on the serum lipid profile and cytokines after the consumption of a plant sterol-enriched beverage with a milk fat globule membrane: a clinical study

2018 ◽  
Vol 9 (10) ◽  
pp. 5209-5219 ◽  
Author(s):  
Andrea Alvarez-Sala ◽  
Virginia Blanco-Morales ◽  
Antonio Cilla ◽  
Ramona Ángeles Silvestre ◽  
Elena Hernández-Álvarez ◽  
...  
Keyword(s):  
Clinical Study ◽  
Lipid Profile ◽  
Milk Fat ◽  
Serum Lipid ◽  
Plant Sterol ◽  
Serum Lipids ◽  
Positive Impact ◽  
Milk Fat Globule Membrane ◽  
Milk Fat Globule ◽  
Fat Globule

Positive impact on serum lipids and cytokines after functional beverage's intake by postmenopausal women.

scholarly journals Milk Fat Globule Membrane Proteome and Micronutrients in the Milk Lipid Fraction: Insights into Milk Bioactive Compounds

Dairy ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 202-217
Author(s):  
Michele Manoni ◽  
Donata Cattaneo ◽  
Sharon Mazzoleni ◽  
Carlotta Giromini ◽  
Antonella Baldi ◽  
...  
Keyword(s):  
Milk Fat ◽  
Lipid Fraction ◽  
Value Added ◽  
Milk Lipid ◽  
Milk Fat Globule Membrane ◽  
Fat Globules ◽  
Milk Fat Globules ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Value Added Products

Milk lipids are composed of milk fat globules (MFGs) surrounded by the milk fat globule membrane (MFGM). MFGM protects MFGs from coalescence and enzymatic degradation. The milk lipid fraction is a “natural solvent” for macronutrients such as phospholipids, proteins and cholesterol, and micronutrients such as minerals and vitamins. The research focused largely on the polar lipids of MFGM, given their wide bioactive properties. In this review we discussed (i) the composition of MFGM proteome and its variations among species and phases of lactation and (ii) the micronutrient content of human and cow’s milk lipid fraction. The major MFGM proteins are shared among species, but the molecular function and protein expression of MFGM proteins vary among species and phases of lactation. The main minerals in the milk lipid fraction are iron, zinc, copper and calcium, whereas the major vitamins are vitamin A, β-carotene, riboflavin and α-tocopherol. The update and the combination of this knowledge could lead to the exploitation of the MFGM proteome and the milk lipid fraction at nutritional, biological or technological levels. An example is the design of innovative and value-added products, such as MFGM-supplemented infant formulas.

PROTEOME OF MILK FAT GLOBULE MEMBRANE AND MAMMARY GLAND TISSUE IN GOAT FED DIFFERENT LIPID SUPPLEMENTATION

2021 ◽  
pp. 106378
Author(s):  
Iolly Tábata Oliveira Marques ◽  
Fábio Roger Vasconcelos ◽  
Juliana Paula Martins Alves ◽  
Assis Rubens Montenegro ◽  
César Carneiro Linhares Fernandes ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Milk Fat ◽  
Milk Fat Globule Membrane ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Milk Fat Globule ◽  
Lipid Supplementation ◽  
Fat Globule

Production and characterization of monoclonal antibodies directed against bovine milk fat globule membrane (MFGM)

1994 ◽  
Vol 1199 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Naohito Aoki ◽  
Hidenori Kuroda ◽  
Miho Urabe ◽  
Yoshimi Taniguchi ◽  
Takahiro Adachi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Milk Fat ◽  
Bovine Milk ◽  
Milk Fat Globule Membrane ◽  
Milk Fat Globule ◽  
Fat Globule

Chemical changes in bovine milk fat globule membrane caused by heat treatment and homogenization of whole milk

2002 ◽  
Vol 69 (4) ◽  
pp. 555-567 ◽  
Author(s):  
SUNG JE LEE ◽  
JOHN W. SHERBON
Keyword(s):  
Heat Treatment ◽  
Membrane Proteins ◽  
Milk Fat ◽  
Whey Proteins ◽  
Bovine Milk ◽  
Chemical Changes ◽  
Milk Fat Globule Membrane ◽  
Whole Milk ◽  
Milk Fat Globule ◽  
Fat Globule

The effects of heat treatment and homogenization of whole milk on chemical changes in the milk fat globule membrane (MFGM) were investigated. Heating at 80 °C for 3–18 min caused an incorporation of whey proteins, especially β-lactoglobulin (β-lg), into MFGM, thus increasing the protein content of the membrane and decreasing the lipid. SDS-PAGE showed that membrane glycoproteins, such as PAS-6 and PAS-7, had disappeared or were weakly stained in the gel due to heating of the milk. Heating also decreased free sulphydryl (SH) groups in the MFGM and increased disulphide (SS) groups, suggesting that incorporation of β-lg might be due to association with membrane proteins via disulphide bonds. In contrast, homogenization caused an adsorption of caseins to the MFGM but no binding of whey proteins to the MFGM without heating. Binding of caseins and whey proteins and loss of membrane proteins were not significantly different between milk samples that were homogenized before and after heating. Viscosity of whole milk was increased when milk was treated with both homogenization and heating.

Variability of Milk Fat Globule Membrane Protein Gene between Cattle and Riverine Buffalo

DNA Sequence ◽  
2004 ◽  
Vol 15 (5-6) ◽  
pp. 326-331 ◽  
Author(s):  
T.K. Bhattacharya ◽  
S.S. Misra ◽  
Feroz D. Sheikh ◽  
S. Dayal ◽  
V. Vohra ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Milk Fat ◽  
Protein Gene ◽  
Milk Fat Globule Membrane ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Membrane Protein Gene
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