scholarly journals MICROPROPAGATION OF RHYNCHOSTYLIS GIGANTEA ORCHID BY SOMATIC EMBRYOGENIC CULTURES

2019 ◽  
Vol 7 ◽  
pp. 969-974
Author(s):  
Tran Van Minh

Rhynchostylis gigantea is a wild orchid species commonly grown. Flowering at the traditional Tet holiday, the flower season always blooms in December of the lunar calendar. The flower has a faint aroma, so it is called Nghinh spring and is a kind of orchid with a national soul and national essence. Rhynchostylis gigantea is found in many areas in Vietnam as it is distributed in the South Central Highlands, especially the areas bordering Laos and Cambodia at low altitudes <600m. Rhynchostylis gigantea is a type of heat-resistant orchid, with suitable temperatures for orchid being from 26-30oC. Rhynchostylis gigantea species have many different colors such as red, white, shrimp brick color, blue and pink. The single-member tree does not assume pseudobulbs, grows vertically, has many pneumatophores and grows straight from the stem. Rhynchostylis gigantea grows slowly and regenerates with seeds. Due to high genetic diversity, it is difficult to maintain the parent’s characteristics. Applying techniques of somatic embryo culture in conservation and development of the Rhynchostylis gigantea tree to meet the increasing demand for seedlings is urgent.

2020 ◽  
Vol 13 ◽  
pp. 194008292094201
Author(s):  
Zongyan Li ◽  
Jing Li ◽  
Minyang Li

Seven populations of Paphiopedilum micranthum from Southeast China were used to assess the influence of human disturbance on genetic structure through analysis with sequence-related amplified polymorphism technology. The results indicated that there was high genetic diversity at species level ( p =  81.25%; I =  0.3709) and a significantly higher differentiation level compared to that those of other outcrossing orchid species, and that moderately disturbed populations sustained higher genetic diversity indexes than the natural populations. This study revealed that human disturbance and population size did not significantly affect the populations’ genetic diversity but aggravated their differentiation. This may suggest that the habitat had a much greater influence on genetic variation.


Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.


2015 ◽  
Vol 86 (1-2) ◽  
pp. 76-84 ◽  
Author(s):  
Tara A. Clarke ◽  
Olivia Gray ◽  
Lisa Gould ◽  
Andrew S. Burrell

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 40
Author(s):  
Evgeny Genelt-Yanovskiy ◽  
Yixuan Li ◽  
Ekaterina Stratanenko ◽  
Natalia Zhuravleva ◽  
Natalia Strelkova ◽  
...  

Ophiura sarsii is a common brittle star species across the Arctic and Sub-Arctic regions of the Atlantic and the Pacific oceans. Ophiurasarsii is among the dominant echinoderms in the Barents Sea. We studied the genetic diversity of O.sarsii by sequencing the 548 bp fragment of the mitochondrial COI gene. Ophiurasarsii demonstrated high genetic diversity in the Barents Sea. Both major Atlantic mtDNA lineages were present in the Barents Sea and were evenly distributed between the northern waters around Svalbard archipelago and the southern part near Murmansk coast of Kola Peninsula. Both regions, and other parts of the O.sarsii range, were characterized by high haplotype diversity with a significant number of private haplotypes being mostly satellites to the two dominant haplotypes, each belonging to a different mtDNA clade. Demographic analyses indicated that the demographic and spatial expansion of O.sarsii in the Barents Sea most plausibly has started in the Bølling–Allerød interstadial during the deglaciation of the western margin of the Barents Sea.


2021 ◽  
Vol 9 (8) ◽  
pp. 1612
Author(s):  
Werner Ruppitsch ◽  
Andjela Nisic ◽  
Patrick Hyden ◽  
Adriana Cabal ◽  
Jasmin Sucher ◽  
...  

In many dairy products, Leuconostoc spp. is a natural part of non-starter lactic acid bacteria (NSLAB) accounting for flavor development. However, data on the genomic diversity of Leuconostoc spp. isolates obtained from cheese are still scarce. The focus of this study was the genomic characterization of Leuconostoc spp. obtained from different traditional Montenegrin brine cheeses with the aim to explore their diversity and provide genetic information as a basis for the selection of strains for future cheese production. In 2019, sixteen Leuconostoc spp. isolates were obtained from white brine cheeses from nine different producers located in three municipalities in the northern region of Montenegro. All isolates were identified as Ln. mesenteroides. Classical multilocus sequence tying (MLST) and core genome (cg) MLST revealed a high diversity of the Montenegrin Ln. mesenteroides cheese isolates. All isolates carried genes of the bacteriocin biosynthetic gene clusters, eight out of 16 strains carried the citCDEFG operon, 14 carried butA, and all 16 isolates carried alsS and ilv, genes involved in forming important aromas and flavor compounds. Safety evaluation indicated that isolates carried no pathogenic factors and no virulence factors. In conclusion, Ln. mesenteroides isolates from Montenegrin traditional cheeses displayed a high genetic diversity and were unrelated to strains deposited in GenBank.


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