scholarly journals TO DEVELOP FORENSIC CRITERIA FOR THE DIFFERENTIAL DIAGNOSIS OF INTRACEREBRAL HEMORRHAGE OF TRAUMATIC AND NON-TRAUMATIC ORIGIN USING MUELLER-MATRIX MICROSCOPY OF LINEAR DICHROISM

2021 ◽  
Vol 2 ◽  
pp. 21-24
Author(s):  
Marta Garazdiuk ◽  
Viktor Bachynskyі ◽  
Olena Nechytailo ◽  
Oleksandr Garazdiuk
Keyword(s):  
Differential Diagnosis ◽  
Intracerebral Hemorrhage ◽  
Causes Of Death ◽  
Brain Infarction ◽  
Linear Dichroism ◽  
Mueller Matrix ◽  
Ischemic Brain ◽  
Satisfactory Level ◽  
Traumatic Origin ◽  
Matrix Invariants

Differential diagnosis of intracerebral hemorrhage due to traumatic and non-traumatic origin is a challenging issue, especially in the absence of visible body injuries and other signs of violence. For a forensic expert-practitioner, the main thing is objectivity, accuracy, and speed of obtaining the result, which could fully satisfy the methods of laser polarimetry in the case of differential diagnosis of hemorrhages of traumatic and non-traumatic origin in the human brain matter. The purpose: To develop forensic criteria for the differential diagnosis of intracerebral hemorrhage of traumatic and non-traumatic origin using Mueller-matrix microscopy of linear dichroism. Materials and methods: The object of the study was brain matter samples, collected in 115 corpses of both sexes aged 22 to 86 years with accurately known causes of death. The causes of death included coronary artery disease, traumatic brain hemorrhage, ischemic brain infarction, and non-traumatic brain hemorrhages. Brain matter samples have been frozen rapidly, and histological sections have been made using a freezing microtome. The slices have been evaluated using the method of azimuthal-invariant Mueller-matrix microscopy, followed by mathematical and statistical processing of the results.  Results: significant topographic heterogeneity of Mueller-matrix invariants of brain matter samples linear dichroism maps among all research groups have been found. Individual and significant variations of dispersion, asymmetry, and kurtosis magnitude have confirmed the heterogeneity of Mueller-matrix invariants. The difference of images and histograms among groups is explained by the presence of necrotic changes in the case of ischemic brain necrosis and blood cells in the brain matter samples. For the set of central statistical moments of the 1st, 3rd, and 4th orders, which characterize the distributions of circular dichroism, the method of MM-microscopy in the differentiation of samples of non-hemorrhage and hemorrhage groups reaches a satisfactory level - 78% - 84%. Conclusions: Brain matter samples Muller-matrix microscopy of linear dichroism is effective for solving diagnostic problems of forensic medicine related to evaluating the cause of death from intracerebral hemorrhage of various origins. Diagnostic efficiency of Mueller-matrix mapping of polarization manifestations of linear dichroism method for intergroup differentiation of samples of deaths from traumatic hemorrhage (Group 2) and ischemic cerebral infarction (Group 3) reaches a satisfactory level of 79 - 84%.

scholarly journals Forensic medical differential diagnosis of brain infarctions and hemorrhasis of traumatic genesis by mueller-matrix microscopy

2021 ◽  
Vol 25 (1(97)) ◽  
pp. 24-30
Author(s):  
M. Garazdiuk ◽  
O. Dubolazov ◽  
V. Tiulienieva
Keyword(s):  
Differential Diagnosis ◽  
Ischemic Stroke ◽  
Circular Dichroism ◽  
Maximum Level ◽  
Study Groups ◽  
Mueller Matrix ◽  
Control Group ◽  
Histological Sections ◽  
Matrix Invariants ◽  
Circular Dichroïsm

Abstract. The aim of the work is to develop forensic criteria for differential diagnosis of traumatic hemorrhages (HTG), ischemic stroke (IS), and hemorrhages of nontraumatic genesis (HNG) formation by 3D Mueller-matrix microscopy of layers of azimuthal-invariant Mueller-matrix images of circular birefringence of histological sections of the brain. Material and methods. Native sections of brain taken from 110 corpses were used for the study in the case of: death from coronary heart disease - 20 (18.1%) native sections (group 1 - control); HTG - 30 (27.3%) sections (group 2), IIB - 30 (27.3%) sections (group 3), HNG - 30 (27.3%) sections (group 4). Measurement of the values of the distribution of coordinate parameters of polarization at the points of microscopic images was performed at the location of the standard Stokes polarimeter. Results. It is found that for each of the phase cross-sections of the field, the volume of distributions of complex amplitudes of sensitivity, specificity and balanced accuracy of statistical analysis of coordinate distributions of Mueller-matrix invariants of circular dichroism have maximum values for small phase shifts corresponding to their level. The maximum level of balanced accuracy of intergroup differentiation was revealed by calculating statistical moments of the 3rd and 4th order, which characterize the asymmetry and excess distributions of the Mueller-matrix invariants values of circular dichroism of histological sections of brain substance. Conclusions. Excellent balanced accuracy (95% - 96%) of differential diagnosis was achieved between the control group and all study groups, good accuracy (92% - 93%) between ischemic stroke and traumatic hemorrhage and satisfactory accuracy (85% - 86%) between traumatic and hemorrhagic strokes genesis.

scholarly journals Use of azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance for diagnosis of hemorrhage genesis

2020 ◽  
Vol 26 (2) ◽  
pp. 62-66
Author(s):  
M.S. Garazdiuk ◽  
O.V. Dubolazov ◽  
S.M. Malanchuk
Keyword(s):  
Cerebral Infarction ◽  
Linear Dichroism ◽  
Mueller Matrix ◽  
Histological Sections ◽  
Satisfactory Level ◽  
Group 4 ◽  
Brain Substance ◽  
Group 3 ◽  
Group 2 ◽  
Traumatic Hemorrhage

Differential diagnosis of the cause of death (CD) from ischemic cerebral infarction (ICI), hemorrhages of traumatic (HTG) and non-traumatic (HNG) genesis exclude the violent nature of death. The aim of our work was to develop forensic criteria for hemorrhage differentiation of traumatic and non-traumatic genesis and ICI by azimuthal-invariant Mueller-matrix images of linear dichroism of histological sections of brain substance (HBS). For the study were used native sections of HBS from 130 corpses in the case of: death from coronary heart disease – 40 of native sections (group 1 – control); HTG – 30 sections (group 2), ICI – 30 native sections (group 3), HNG – 30 native sections (group 4). Measuring the coordinate allocation meanings of parameters of polarization in the points of microscopic images was carried out at the location of the standard stokes-polarimeter. The effectiveness of intergroup differentiation of samples of deaths from traumatic hemorrhage and ischemic cerebral infarction reaches a satisfactory level and is 76-83%. Efficiency between group differentiation of samples of deaths from nontraumatic and traumatic hemorrhages reaches a satisfactory level and is 75-82%. As for differentiation between ischemic cerebral infarction and nontraumatic hemorrhages thise method is ineffective.

scholarly journals Mueller-matrix differential diagnosis of biological crystallites phase anisotropy

2013 ◽  
Author(s):  
V. O. Ushenko ◽  
G. D. Koval ◽  
V. T. Bachinskiy ◽  
L. Ya. Kushnerick ◽  
M. Garazdiyk ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Mueller Matrix ◽  
Phase Anisotropy ◽  
Matrix Differential

Abstract 2276: Microhemorrhage Burden Increases the Risk of Hematoma Growth in Patients with Intracerebral Hemorrhage

Stroke ◽  
2012 ◽  
Vol 43 (suppl_1) ◽  
Author(s):  
Joan Martí-Fàbregas ◽  
Estrella Morenas ◽  
Raquel Delgado-Mederos ◽  
Lavinia Dinia ◽  
Esther Granell ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Statistical Analysis ◽  
Intracerebral Hemorrhage ◽  
Multicentre Study ◽  
Symptom Onset ◽  
Variable Time ◽  
Hematoma Growth ◽  
Acute Intracerebral Hemorrhage ◽  
Radiological Studies

Introduction Microhemorrhages (MH) are lesions detected on radiological studies resulting from an underlying small-vessel angiopathy. We assesed the hypothesis that the presence of MH increases the risk of hematoma growth (HG) in patients with acute Intracerebral Hemorrhage (ICH). Methods We evaluated a series of patients in a prospective and multicentre study. We included patients with a spontaneous supratentorial ICH within the first 6 hours after symptom onset, that also had a follow-up CT 24-72 hours later and a MRI performed after a variable time after ICH. HG was defined as an increase >33% in the volume of hematoma on the follow-up CT, in comparison with the admission CT. The volume was calculated using the formula AxBxC/2. On MR scans we assessed the presence, number and distribution of MH. After differential diagnosis with other radiological lesions, MH were evaluated on echo-gradient sequences and defined as hypointense rounded lesions with a diameter <10mm. Statistical analysis: Bivariate tests with the whole sample and with the subgroup of patients with less than 3 hours from symptom onset. Results We studied 46 patients, whose mean age was 68.8±11.2 y and 68% were men. Mean baseline volume was 19.1±27.3 cc. We detected MH in 7/15 patients with HG and in 18/31 patients without HG (46.7% vs 58.1%, p=0.53). In the subgroup of patients with 10 MH, the risk of HG was higher than in patients with 0-10 MH (75% vs 28.6%, p=0.067), and this difference was significant when considering only patients with a <3 hours evolution (100% vs 31%, p=0.044). We did not observe any association between risk of HG and distribution of MH. Age and time to CT were equivalent in the two groups (with and without HG), either in the <6 or <3 hours subgroups. Conclusions In conclusion, in patients with hyperacute ICH, the presence of more than 10 MH increases the risk of HG. This is probably an indirect marker of a more severe underlying angiopathy.

scholarly journals Ischemic Brain Injury After Intracerebral Hemorrhage

Stroke ◽  
2012 ◽  
Vol 43 (8) ◽  
pp. 2258-2263 ◽  
Author(s):  
Shyam Prabhakaran ◽  
Andrew M. Naidech
Keyword(s):  
Brain Injury ◽  
Intracerebral Hemorrhage ◽  
Ischemic Brain Injury ◽  
Ischemic Brain

scholarly journals Polarization-Singular Approach to Imaging Mueller-Matrix Polarimetry in the Differential Diagnosis of Histological Sections of Biopsy of Tumors of the Uterus and Prostate

2021 ◽  
Vol 9 ◽  
Author(s):  
A. Dubolazov ◽  
V. Ushenko ◽  
L. Trifonyuk ◽  
A. Stashkevich ◽  
I. Soltys ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Mueller Matrix ◽  
Evidence Based ◽  
Malignant Tumours ◽  
Diagnostic Use ◽  
Matrix Mapping ◽  
Adenocarcinoma Of The Prostate ◽  
Characteristic Values ◽  
Sensitivity Specificity ◽  
Based Medicine

The possibilities of the diagnostic use of the singular approach of the distributions of the number of characteristic values of the MMI is effective for differentiating the polarization properties of histological biopsy sections of benign and malignant tumours of the uterus and prostate. Within the framework of evidence-based medicine, the sensitivity, specificity and accuracy of the azimuthal-invariant express (∼15 min) method of Mueller-matrix mapping of polarization-singular states in the differential diagnosis of uterine myoma and adenocarcinoma, as well as adenocarcinoma of the prostate with varying degrees of differentiation have been determined.

scholarly journals A Combined Treatment with Tacrolimus (FK506) and Recombinant Tissue Plasminogen Activator for Thrombotic Focal Cerebral Ischemia in Rats: Increased Neuroprotective Efficacy and Extended Therapeutic Time Window

2002 ◽  
Vol 22 (10) ◽  
pp. 1205-1211 ◽  
Author(s):  
Masashi Maeda ◽  
Yasuhisa Furuichi ◽  
Noriko Ueyama ◽  
Akira Moriguchi ◽  
Natsuki Satoh ◽  
...  
Keyword(s):  
Brain Damage ◽  
Time Window ◽  
Brain Infarction ◽  
Combined Treatment ◽  
Recombinant Tissue Plasminogen Activator ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Mca Occlusion ◽  
Tissue Plasminogen ◽  
Therapeutic Time Window

The authors evaluated the therapeutic efficacy of tacrolimus (FK506), administered alone or in combination with recombinant tissue plasminogen activator (t-PA), on brain infarction following thrombotic middle cerebral artery (MCA) occlusion. Thrombotic occlusion of the MCA was induced by a photochemical reaction between rose bengal and green light in Sprague-Dawley rats, and the volume of ischemic brain damage was determined 24 hours later. Intravenous administration of tacrolimus or t-PA dose-dependently reduced the volume of ischemic brain infarction, whether administered immediately or 1 hour after MCA occlusion. When tacrolimus or t-PA was administered 2 hours after MCA occlusion, each drug showed a tendency to reduce ischemic brain damage. However, combined treatment with both drugs resulted in a significant reduction in ischemic brain damage. On administration 3 hours after MCA occlusion, tacrolimus alone showed no effect, and t-PA tended to worsen ischemic brain damage. However, the combined treatment with both drugs not only ameliorated the worsening trend seen with t-PA alone, but also tended to reduce ischemic brain damage. In conclusion, tacrolimus, used in combination with t-PA, augmented therapeutic efficacy on brain damage associated with focal ischemia and extended the therapeutic time window compared to single-drug treatments.

scholarly journals Forensic Aspects of Perinatal Deaths

2018 ◽  
Vol 8 (3) ◽  
pp. 452-491 ◽  
Author(s):  
Marta C. Cohen ◽  
Irene Scheimberg
Keyword(s):  
Differential Diagnosis ◽  
Child Abuse ◽  
Causes Of Death ◽  
Fetal Death ◽  
Correct Interpretation ◽  
Perinatal Deaths

From a forensic pathologist’s perspective, there are several aspects of the perinatal postmortem that are particularly important. If a fetus is found abandoned, the pathologist needs to ascertain the fetal age, the appropriateness of growth, if the baby was born alive or dead, and the possible causes of death. In cases of litigation for perinatal deaths occurring in hospitals, access to the obstetric and neonatal notes (if the baby is born alive and dies a few hours or days later) is fundamental to reach a correct interpretation and conclusion. The most important points to consider in cases of intrapartum death are the roles of asphyxia and trauma in the causation of the baby’s death. Timing of the fetal death in relation to delivery may also be an important point in these cases. Finally, intrapartum lesions should always be considered in the differential diagnosis of possible child abuse in babies aged two months or less.

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