scholarly journals Forensic medical differential diagnosis of brain infarctions and hemorrhasis of traumatic genesis by mueller-matrix microscopy

2021 ◽  
Vol 25 (1(97)) ◽  
pp. 24-30
Author(s):  
M. Garazdiuk ◽  
O. Dubolazov ◽  
V. Tiulienieva
Keyword(s):  
Differential Diagnosis ◽  
Ischemic Stroke ◽  
Circular Dichroism ◽  
Maximum Level ◽  
Study Groups ◽  
Mueller Matrix ◽  
Control Group ◽  
Histological Sections ◽  
Matrix Invariants ◽  
Circular Dichroïsm

Abstract. The aim of the work is to develop forensic criteria for differential diagnosis of traumatic hemorrhages (HTG), ischemic stroke (IS), and hemorrhages of nontraumatic genesis (HNG) formation by 3D Mueller-matrix microscopy of layers of azimuthal-invariant Mueller-matrix images of circular birefringence of histological sections of the brain. Material and methods. Native sections of brain taken from 110 corpses were used for the study in the case of: death from coronary heart disease - 20 (18.1%) native sections (group 1 - control); HTG - 30 (27.3%) sections (group 2), IIB - 30 (27.3%) sections (group 3), HNG - 30 (27.3%) sections (group 4). Measurement of the values of the distribution of coordinate parameters of polarization at the points of microscopic images was performed at the location of the standard Stokes polarimeter. Results. It is found that for each of the phase cross-sections of the field, the volume of distributions of complex amplitudes of sensitivity, specificity and balanced accuracy of statistical analysis of coordinate distributions of Mueller-matrix invariants of circular dichroism have maximum values for small phase shifts corresponding to their level. The maximum level of balanced accuracy of intergroup differentiation was revealed by calculating statistical moments of the 3rd and 4th order, which characterize the asymmetry and excess distributions of the Mueller-matrix invariants values of circular dichroism of histological sections of brain substance. Conclusions. Excellent balanced accuracy (95% - 96%) of differential diagnosis was achieved between the control group and all study groups, good accuracy (92% - 93%) between ischemic stroke and traumatic hemorrhage and satisfactory accuracy (85% - 86%) between traumatic and hemorrhagic strokes genesis.

scholarly journals Forensic medical evaluation of hemorrhages into the brain of traumatic and non-traumatic genesis by the Mueller matrix microscopy of circular dichroism

2021 ◽  
Vol 25 (2(98)) ◽  
pp. 29-34
Author(s):  
M. Garazdiuk ◽  
O. Dubolazov
Keyword(s):  
Circular Dichroism ◽  
Mueller Matrix ◽  
Control Group ◽  
Coronary Insufficiency ◽  
Microscopic Images ◽  
Histological Sections ◽  
Brain Substance ◽  
The Dead ◽  
Circular Dichroïsm ◽  
The Brain

The aim of the work is to develop forensic criteria for differentiation of hemorrhages of traumatic and non-traumatic genesis by digital Mueller matrix (MM) polarization microscopy of histological sections of human brain substance (HBS).Material and methods. Native histological preparations of HBS from 32 corpses with ischemic stroke (1 group), 35 corpses with hemorrhages of traumatic origin (2 groups), 33 corpses with hemorrhages in HBS of non-traumatic genesis (3 group) and 30 corpses caused by acute coronary insufficiency (4 control group). The method of research is azimuthal-invariant MM microscopy of circular dichroism (CD) of histological sections of the brain.Results. The results of studies of the coordinate and statistical structure of maps of the size of values at the points of digital microscopic images of histological sections of HBS of the dead of all groups revealed a coordinate-inhomogeneous structure of all distributions of the size of the CD of microscopic images of histological sections of the dead of all groups. Probable distributions that characterize MM invariants CD maps of histological sections of brain samples from all groups have a small scatter of values of the statistical moment of the 2nd order, as well as significant values of statistical moments of the 3rd and 4th orders.Conclusion. The efficiency of using the Mueller-matrix mapping method in diagnosing of hemorrhage genesis in the brain substance in the differentiation of the set of samples of control and experimental groups reaches a satisfactory level - 77% - 78%.

scholarly journals Optical Chirality Determined from Mueller Matrices

2021 ◽  
Vol 11 (15) ◽  
pp. 6742
Author(s):  
Hans Arwin ◽  
Stefan Schoeche ◽  
James Hilfiker ◽  
Mattias Hartveit ◽  
Kenneth Järrendahl ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Constitutive Relations ◽  
Mueller Matrix ◽  
Optical Path ◽  
Electromagnetic Modeling ◽  
Structural Effects ◽  
Polarization Effects ◽  
Optical Chirality ◽  
Mueller Matrices ◽  
Circular Dichroïsm

Optical chirality, in terms of circular birefringence and circular dichroism, is described by its electromagnetic and magnetoelectric material tensors, and the corresponding optical activity contributes to the Mueller matrix. Here, spectroscopic ellipsometry in the spectral range 210–1690 nm is used to address chiral phenomena by measuring Mueller matrices in transmission. Three approaches to determine chirality parameters are discussed. In the first approach, applicable in the absence of linear polarization effects, circular birefringence and circular dichroism are evaluated directly from elements of a Mueller matrix. In the second method, differential decomposition is employed, which allows for the unique separation of chirality parameters from linear anisotropic parameters as well as from depolarization provided that the sample is homogeneous along the optical path. Finally, electromagnetic modeling using the Tellegen constitutive relations is presented. The last method also allows structural effects to be included. The three methods to quantify optical chirality are demonstrated for selected materials, including sugar solutions, α-quartz, liquid crystals, beetle cuticle, and films of cellulose nanocrystals.

scholarly journals Usefulness of Selected Acute-Phase Proteins in the Postsurgical Monitoring of Arthroscopy and Splint Bone Removal in Horses

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2952
Author(s):  
Maciej Przewoźny ◽  
Magdalena Senderska-Płonowska ◽  
Anna Rząsa ◽  
Heliodor Wierzbicki ◽  
Jacek Borkowski ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Proteinase Inhibitors ◽  
Maximum Level ◽  
Study Groups ◽  
Orthopedic Procedures ◽  
Control Group ◽  
Complicated Case ◽  
Bone Removal ◽  
Serum Haptoglobin

Background: Arthroscopy and splint bone removal are the common orthopedic procedures in horses. Estimation of the dynamics of acute phase proteins in postoperative monitoring seems to be interesting diagnostic approach. The aim of the study was to investigate changes in the concentrations of plasma inflammatory markers—fibrinogen, haptoglobin, and protease inhibitors—following orthopedic surgery in horses. The study involved 114 horses, divided into two study groups undergoing: arthroscopy (41 horses) and splint bone removal (13 horses). The control group consisted of 60 healthy horses. The blood was collected before the surgery and 24, 48, 72 h, 5, 7, 10, 14 and 28 days after the surgery. Plasma fibrinogen, serum haptoglobin and proteinase inhibitors were measured. Results: In non-complicated cases of arthroscopy and splint bone removal, fibrinogen and haptoglobin increased stepwise from 24 h, achieved the maximum level at 72 h and returned to preoperative levels after 10–14 days. In one complicated case after arthroscopy surgery the marked increase in fibrinogen and haptoglobin concentrations was observed 24 h earlier than standard parameters of inflammation Conclusion: The study shows the evolution of APPs after arthroscopy and splint bone removal in 28 days postsurgery period and in the case of one complicated case of arthroscopy.

scholarly journals THE ANALYSIS OF METHYLATION OF DNA PROMOTER OF SFRP2 GENE IN PATIENTS WITH HYPERPLASTIC PROCESSES OF THE ENDOMETRIUM

2018 ◽  
Vol 40 (2) ◽  
pp. 109-113
Author(s):  
V G Marichereda ◽  
N A Bykovа ◽  
V V Bubnov ◽  
G S Manasova ◽  
T Y Moskalenko ◽  
...  
Keyword(s):  
Gene Promoter ◽  
Maximum Level ◽  
Intraepithelial Neoplasia ◽  
Study Groups ◽  
Control Group ◽  
Healthy Women ◽  
Methylation Of Dna ◽  
Specific Pcr ◽  
Endometrial Intraepithelial Neoplasia ◽  
Specific Pcr Assay

The hyperplastic processes of the endometrium can arise not only against the background of excessive influence of estrogen, but also against the background of epigenetic damages that affect apoptosis, cell proliferation, differentiation, and adhesion, and DNA reparation. The aim of our study was to investigate and analyze the status of methylation of the promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium. Materials and Methods: The study groups were the following: I — patients with endometrial hyperplasia (EH, n = 9); II — patients with endometrial intraepithelial neoplasia (EIN, n = 10), III — control groups: 1) with endometrial cancer (EC, n = 4), and 2) healthy women (n = 4). Determination of promoter methylation of SFRP2 gene was carried out by the semiquantitative method of methylation-specific PCR assay. Results: The maximum level of methylation of SFRP2 gene promoter had been revealed in patients with EC — 42.80 ± 3.55% (р < 0.05). The patients of the I group had the lowest values of methylation of SFRP2 gene promoter — 10.66 ± 0.85%, while in patients of the II group this indicator was higher — 20.60 ± 0.95% (р < 0.05). In healthy women of the control group, methylation of SFRP2 gene promoter was detected in none of the samples. Conclusion: The content of the methylated SFRP2 gene in endometrial tissue of patients with hyperplastic processes higher than 20–25% allows relate these women to the risk group of EC development and dictates the need of intensive observation of such patients.

scholarly journals TO DEVELOP FORENSIC CRITERIA FOR THE DIFFERENTIAL DIAGNOSIS OF INTRACEREBRAL HEMORRHAGE OF TRAUMATIC AND NON-TRAUMATIC ORIGIN USING MUELLER-MATRIX MICROSCOPY OF LINEAR DICHROISM

2021 ◽  
Vol 2 ◽  
pp. 21-24
Author(s):  
Marta Garazdiuk ◽  
Viktor Bachynskyі ◽  
Olena Nechytailo ◽  
Oleksandr Garazdiuk
Keyword(s):  
Differential Diagnosis ◽  
Intracerebral Hemorrhage ◽  
Causes Of Death ◽  
Brain Infarction ◽  
Linear Dichroism ◽  
Mueller Matrix ◽  
Ischemic Brain ◽  
Satisfactory Level ◽  
Traumatic Origin ◽  
Matrix Invariants

Differential diagnosis of intracerebral hemorrhage due to traumatic and non-traumatic origin is a challenging issue, especially in the absence of visible body injuries and other signs of violence. For a forensic expert-practitioner, the main thing is objectivity, accuracy, and speed of obtaining the result, which could fully satisfy the methods of laser polarimetry in the case of differential diagnosis of hemorrhages of traumatic and non-traumatic origin in the human brain matter. The purpose: To develop forensic criteria for the differential diagnosis of intracerebral hemorrhage of traumatic and non-traumatic origin using Mueller-matrix microscopy of linear dichroism. Materials and methods: The object of the study was brain matter samples, collected in 115 corpses of both sexes aged 22 to 86 years with accurately known causes of death. The causes of death included coronary artery disease, traumatic brain hemorrhage, ischemic brain infarction, and non-traumatic brain hemorrhages. Brain matter samples have been frozen rapidly, and histological sections have been made using a freezing microtome. The slices have been evaluated using the method of azimuthal-invariant Mueller-matrix microscopy, followed by mathematical and statistical processing of the results.  Results: significant topographic heterogeneity of Mueller-matrix invariants of brain matter samples linear dichroism maps among all research groups have been found. Individual and significant variations of dispersion, asymmetry, and kurtosis magnitude have confirmed the heterogeneity of Mueller-matrix invariants. The difference of images and histograms among groups is explained by the presence of necrotic changes in the case of ischemic brain necrosis and blood cells in the brain matter samples. For the set of central statistical moments of the 1st, 3rd, and 4th orders, which characterize the distributions of circular dichroism, the method of MM-microscopy in the differentiation of samples of non-hemorrhage and hemorrhage groups reaches a satisfactory level - 78% - 84%. Conclusions: Brain matter samples Muller-matrix microscopy of linear dichroism is effective for solving diagnostic problems of forensic medicine related to evaluating the cause of death from intracerebral hemorrhage of various origins. Diagnostic efficiency of Mueller-matrix mapping of polarization manifestations of linear dichroism method for intergroup differentiation of samples of deaths from traumatic hemorrhage (Group 2) and ischemic cerebral infarction (Group 3) reaches a satisfactory level of 79 - 84%.

scholarly journals FORENSIC MEDICAL CRITERIA OF DIFFERENTIAL DIAGNOSIS OF ALCOHOL AND CARBON MONOXIDE POISONING BY USING AZIMUTHAL-INVARIANT POLARIZATION MUELLER-MATRIX MICROSCOPY

2021 ◽  
Vol 2 ◽  
pp. 33-40
Author(s):  
Olexandr Harazdiuk ◽  
Ihor Ivaskevich ◽  
Oleh Vanchuliak ◽  
Yuliia Sarkisova
Keyword(s):  
Carbon Monoxide ◽  
Differential Diagnosis ◽  
Cause Of Death ◽  
Human Body ◽  
Carbon Monoxide Poisoning ◽  
Biological Tissues ◽  
Mueller Matrix ◽  
Histological Sections ◽  
Common Causes ◽  
Characteristic Features

Introduction: Fatal intoxications with alcohol and carbon monoxide are the most common causes of poisoning that occur in forensic medical practice. Therefore, the differential diagnosis of these intoxications is extremely important. To solve this problem, we propose the use of the method of azimuthal-invariant polarization Mueller-matrix microscopy of histological sections of biological tissues (BT). Objectives: The purpose of the study was to establish statistically significant criteria for differentiation of the cause of death in case of alcohol poisoning (AP) and carbon monoxide poisoning (CMP) by using azimuthal-invariant polarization Mueller-matrix microscopy of polycrystalline fractions of histological sections of organs and blood films of the human body. Results: A number of characteristic features have been identified that have not been previously described and allow to objectively differentiate the cause of death in the case of AP and CMP, namely Ek – 0.95±0.045 and 1.81±0.092 in the study of histological sections of the myocardium;  Ek – 0,19±0,011 and 1,11±0,094 – polycrystalline blood films. Analysis of the obtained data revealed a good level of accuracy in the study of histological sections of the myocardium (As 85%) and polycrystalline blood films (As 88%). Conclusion: Thus, developed and tested method of azimuthal-invariant polarization Mueller-matrix microscopy allows to differentiate the cause of death in cases of AP and CMP.

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

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