Evaluation of Cytotoxicity on Human Cancer Cells and Antioxidant and Immune Cell Activities of Codonopsis lanceolata Root Extracts Stored at Different Temperatures and for Different Durations

2019 ◽  
Vol 37 (1) ◽  
Keyword(s):  
Cancer Cells ◽  
Immune Cell ◽  
Human Cancer ◽  
Codonopsis Lanceolata ◽  
Root Extracts ◽  
Human Cancer Cells ◽  
Different Temperatures

scholarly journals Chimeric antigen receptors that trigger phagocytosis

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Meghan A Morrissey ◽  
Adam P Williamson ◽  
Adriana M Steinbach ◽  
Edward W Roberts ◽  
Nadja Kern ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Immune Cell ◽  
Human Cancer ◽  
Antibody Fragment ◽  
Cell Number ◽  
Antigen Receptors ◽  
Chimeric Antigen Receptors ◽  
Cell Therapies ◽  
Human Cancer Cells

Chimeric antigen receptors (CARs) are synthetic receptors that reprogram T cells to kill cancer. The success of CAR-T cell therapies highlights the promise of programmed immunity and suggests that applying CAR strategies to other immune cell lineages may be beneficial. Here, we engineered a family of Chimeric Antigen Receptors for Phagocytosis (CAR-Ps) that direct macrophages to engulf specific targets, including cancer cells. CAR-Ps consist of an extracellular antibody fragment, which can be modified to direct CAR-P activity towards specific antigens. By screening a panel of engulfment receptor intracellular domains, we found that the cytosolic domains from Megf10 and FcRɣ robustly triggered engulfment independently of their native extracellular domain. We show that CAR-Ps drive specific engulfment of antigen-coated synthetic particles and whole human cancer cells. Addition of a tandem PI3K recruitment domain increased cancer cell engulfment. Finally, we show that CAR-P expressing murine macrophages reduce cancer cell number in co-culture by over 40%.

scholarly journals Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 113
Author(s):  
Pablo Cabezas-Sainz ◽  
Carlos Coppel ◽  
Alba Pensado-López ◽  
Pedro Fernandez ◽  
Laura Muinelo-Romay ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Incubation Temperature ◽  
Human Cancer ◽  
Effect Of Temperature ◽  
Zebrafish Embryos ◽  
Morphological Abnormalities ◽  
Human Cancer Cells ◽  
Developmental Effects ◽  
Different Temperatures ◽  
Incubation Temperatures

Published studies show that most of the human cancer xenograft studies in zebrafish embryos have used incubation temperatures in the range of 32–34 °C for 3–6 days post-injection, trying to find a compromise temperature between the zebrafish embryos (28 °C) and the human injected cells (37 °C). While this experimental setup is widely used, a question remains: is possible to overcome the drawbacks caused by a suboptimal temperature for the injected cells? To clarify the effect of temperature and injected cells on the host, in this study, we analyzed the development and health of the last in response to different temperatures in the presence or absence of injected human cancer cells. Comparing different incubation temperatures (28, 34 and 36 °C), we determined morphological abnormalities and developmental effects in injected and non-injected embryos at different time points. Besides this, the expression of selected genes was determined by qPCR to determine temperature affected metabolic processes in the embryos. The results indicate that an incubation temperature of 36 °C during a period of 48 h is suitable for xenotransplantation without morphological or metabolic changes that could be affecting the host or the injected cells, allowing them to proliferate near their optimal temperature.

Indirubin derivatives inhibit SFKs/Stat signaling associated with apoptosis in human cancer cells

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
S Nam ◽  
R Buettner ◽  
X Liu ◽  
J Turkson ◽  
D Kim ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Stat Signaling

Antiproliferation Effects of Germinated-Korean Rough Rice Extract on Human Cancer Cells

2010 ◽  
Vol 39 (3) ◽  
pp. 325-330 ◽  
Author(s):  
Hyun-Young Kim ◽  
In-Guk Hwang ◽  
Eun-Mi Joung ◽  
Tae-Myoung Kim ◽  
Dae-Joong Kim ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Rough Rice
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