Functional Tissue Engineering for Soft Tissue Repair : Matching In Vivo Biomechanics(International Workshop 3)

2006 ◽  
Vol 2005.18 (0) ◽  
pp. 4-5
Author(s):  
David L. Butler ◽  
Natalia Juncosa-Melvin ◽  
John West ◽  
Jason Shearn ◽  
Marc Galloway ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Tissue Repair ◽  
International Workshop ◽  
Soft Tissue Repair ◽  
Functional Tissue Engineering ◽  
Functional Tissue

Functional Tissue Engineering: The Role of Biomechanics

2000 ◽  
Vol 122 (6) ◽  
pp. 570-575 ◽  
Author(s):  
David L. Butler ◽  
Steven A. Goldstein ◽  
Farshid Guilak
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physical Factors ◽  
Cellular Activity ◽  
Biosynthetic Activity ◽  
Functional Tissue Engineering ◽  
Functional Tissue ◽  
Engineered Tissues

“Tissue engineering” uses implanted cells, scaffolds, DNA, protein, and/or protein fragments to replace or repair injured or diseased tissues and organs. Despite its early success, tissue engineers have faced challenges in repairing or replacing tissues that serve a predominantly biomechanical function. An evolving discipline called “functional tissue engineering” (FTE) seeks to address these challenges. In this paper, the authors present principles of functional tissue engineering that should be addressed when engineering repairs and replacements for load-bearing structures. First, in vivo stress/strain histories need to be measured for a variety of activities. These in vivo data provide mechanical thresholds that tissue repairs/replacements will likely encounter after surgery. Second, the mechanical properties of the native tissues must be established for subfailure and failure conditions. These “baseline data” provide parameters within the expected thresholds for different in vivo activities and beyond these levels if safety factors are to be incorporated. Third, a subset of these mechanical properties must be selected and prioritized. This subset is important, given that the mechanical properties of the designs are not expected to completely duplicate the properties of the native tissues. Fourth, standards must be set when evaluating the repairs/replacements after surgery so as to determine, “how good is good enough?” Some aspects of the repair outcome may be inferior, but other mechanical characteristics of the repairs and replacements might be suitable. New and improved methods must also be developed for assessing the function of engineered tissues. Fifth, the effects of physical factors on cellular activity must be determined in engineered tissues. Knowing these signals may shorten the iterations required to replace a tissue successfully and direct cellular activity and phenotype toward a desired end goal. Finally, to effect a better repair outcome, cell-matrix implants may benefit from being mechanically stimulated using in vitro “bioreactors” prior to implantation. Increasing evidence suggests that mechanical stress, as well as other physical factors, may significantly increase the biosynthetic activity of cells in bioartificial matrices. Incorporating each of these principles of functional tissue engineering should result in safer and more efficacious repairs and replacements for the surgeon and patient. [S0148-0731(00)00206-5]

Synthesis and characterization of biodegradable lysine-based waterborne polyurethane for soft tissue engineering applications

2016 ◽  
Vol 4 (11) ◽  
pp. 1682-1690 ◽  
Author(s):  
Hongye Hao ◽  
Jingyu Shao ◽  
Ya Deng ◽  
Shan He ◽  
Feng Luo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Waterborne Polyurethane ◽  
Synthesis And Characterization ◽  
Soft Tissue Repair ◽  
Waterborne Polyurethanes ◽  
Soft Tissue Engineering

Light-crosslinking waterborne polyurethanes (LWPUs) based on lysine possess appropriate elasticity for soft tissue repair, and can induce macrophages into a wound healing phenotype.

Role of Biomechanics in Functional Tissue Engineering

2004 ◽  
Vol 844 ◽  
Author(s):  
Kai-Nan An
Keyword(s):  
Tissue Engineering ◽  
Strategic Information ◽  
Functional Tissue Engineering ◽  
Functional Tissue ◽  
Before And After ◽  
The Matrix ◽  
Design And Manufacture ◽  
Stimulation Of

ABSTRACTFunctional tissue engineering establishes functional criteria for design and manufacture of the scaffold matrix for repair and replacement. It also provides useful and strategic information in mechanical stimulation of the cells seeded in the matrix before and after surgical placement to enhance the success of tissue engineering. Biomechanics plays an important role in accomplishing these requirements by assessing the in vivo environment and the material properties.

scholarly journals A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Hongqing Chen ◽  
Fei Fei ◽  
Xinda Li ◽  
Zhenguo Nie ◽  
Dezhi Zhou ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cell Viability ◽  
Tissue Repair ◽  
Three Dimensional ◽  
Water Retention Capacity ◽  
3D Bioprinting ◽  
Soft Tissue Repair ◽  
Retention Capacity

Abstract Both of the long-term fidelity and cell viability of three-dimensional (3D)-bioprinted constructs are essential to precise soft tissue repair. However, the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs, and bioinks containing excessive polymer are detrimental to cell viability. Here, we obtained a facile hydrogel by introducing 1% aldehyde hyaluronic acid (AHA) and 0.375% N-carboxymethyl chitosan (CMC), two polysaccharides with strong water absorption and water retention capacity, into classic gelatin (GEL, 5%)–alginate (ALG, 1%) ink. This GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/ALG. We fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at 37°C. The printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3% calcium chloride for only 20 s. Flow cytometry results showed that the cell viability was 91.38 ± 1.55% on day 29, and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.06 ± 1.24%. This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair.

Response of endothelial cells and pericytes to hypoxia and erythropoietin in a co-culture assay dedicated to soft tissue repair

2015 ◽  
Vol 407 (1-2) ◽  
pp. 29-40 ◽  
Author(s):  
Gerrit Schneider ◽  
Monika Bubel ◽  
Tim Pohlemann ◽  
Martin Oberringer
Keyword(s):  
Endothelial Cells ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Soft Tissue Repair

scholarly journals Dynamics of hip joint effusion after posterior soft tissue repair in total hip arthroplasty

2006 ◽  
Vol 30 (4) ◽  
pp. 233-236 ◽  
Author(s):  
Sarunas Tarasevicius ◽  
Uldis Kesteris ◽  
Romas Jonas Kalesinskas ◽  
Hans Wingstrand
Keyword(s):  
Soft Tissue ◽  
Total Hip Arthroplasty ◽  
Hip Joint ◽  
Hip Arthroplasty ◽  
Tissue Repair ◽  
Joint Effusion ◽  
Soft Tissue Repair ◽  
Total Hip ◽  
Posterior Soft Tissue

scholarly journals Fixation Strength of Polyetheretherketone Sheath-and-Bullet Device for Soft Tissue Repair in the Foot and Ankle

2018 ◽  
Vol 57 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Jay Christensen ◽  
Brian Fischer ◽  
Michael Nute ◽  
Robert Rizza
Keyword(s):  
Soft Tissue ◽  
Tissue Repair ◽  
Fixation Strength ◽  
Foot And Ankle ◽  
Soft Tissue Repair

A Paradigm for Functional Tissue Engineering

2000 ◽  
Author(s):  
David L. Butler
Keyword(s):  
Tissue Engineering ◽  
Tendon Repair ◽  
National Committee ◽  
New Paradigm ◽  
Functional Tissue Engineering ◽  
Functional Tissue ◽  
Tissue Engineer ◽  
Surgical Implants ◽  
The Us

Abstract Clinicians, biologists, and engineers face difficult challenges in engineering effective, cell-based composites for repair of orthopaedic and cardiovascular tissues. Whether repairing articular cartilage, bone, or blood vessel, the demands placed on the surgical implants can threaten the long-term success of the procedure. In 1998, the US National Committee on Biomechanics addressed this problem by suggesting a new paradigm for tissue engineering called “functional tissue engineering” or FTE. FTE seeks to address several important questions. What are the biomechanical demands placed upon the normal tissue and hence the tissue engineered implant after surgery? What parameters should a tissue engineer design into the implant before surgery? And what biomechanical parameters should the tissue engineer track to determine if the resulting repair is successful? To illustrate the principles, this presentation will discuss tendon repair as a model system for functional tissue engineering.

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