7C22 Development of novel biochip equipped with microfluidic concentration step generator

2012 ◽  
Vol 2012.24 (0) ◽  
pp. _7C22-1_-_7C22-2_
Author(s):  
Shunsuke MATSUI ◽  
Eijiro MAEDA ◽  
Emilie WEIBULL ◽  
Helene ANDERSSON-SVAHN ◽  
Toshiro OHASHI
Keyword(s):  
Concentration Step ◽  
Step Generator

Two Enzyme Immunoassays to Screen for 2,4-Dichlorophenoxyacetic Acid in Water

1987 ◽  
Vol 70 (5) ◽  
pp. 874-878 ◽  
Author(s):  
James Fleeker
Keyword(s):  
Limit Of Detection ◽  
Solid Phase ◽  
Dichlorophenoxyacetic Acid ◽  
Carrier Protein ◽  
Similar Response ◽  
Enzyme Immunoassays ◽  
Concentration Step ◽  
Chlorophenoxyacetic Acid ◽  
Phenoxy Herbicides ◽  
2,4 Dichlorophenoxyacetic Acid

Abstract Two solid-phase enzyme immunoassays were developed to measure 2,4-dichlorophenoxyacetic acid (2,4-D), using 2 sets of structurally distinct immunogens and enzyme ligands. The 2,4-D analog, 2-methyl- 4-chlorophenoxyacetic acid (MCPA), gave a similar response with both methods, whereas other phenoxy herbicides cross-reacted differently. In method A, the aromatic moiety of 2,4-D was distal from the carrier protein and labeled enzyme, whereas in method B, the acetic acid portion of the herbicide was distal. The use of both methods to screen for this herbicide in ground water and municipal and river water reduced the number of false-positive responses. Water sources having a low background response could be monitored with either method alone. When a concentration step, with disposable C18 extraction columns, was used, the limit of sensitivity was 5 ng/L,. Method A was the more sensitive of the 2 methods with a limit of detection of 10 j*g/L without the concentration step

scholarly journals Concentration of Sonication Fluid through Centrifugation Is Superior to Membrane Filtration for Microbial Diagnosis of Orthopedic Implant-Associated Infection

2015 ◽  
Vol 54 (3) ◽  
pp. 788-790 ◽  
Author(s):  
Renato Zitron ◽  
Tali Wajsfeld ◽  
Giselle Burlamaqui Klautau ◽  
Cely Barreto da Silva ◽  
Stanley Nigro ◽  
...  
Keyword(s):  
Membrane Filtration ◽  
Orthopedic Implant ◽  
Microbial Identification ◽  
Concentration Step

Microbial identification of orthopedic implant-associated infections using sonication fluid (SF) submitted to a concentration step by membrane filtration (SMF) was compared with the standard centrifugation (SC) method. Among 33 retrieved infected implants, sonication identified microorganisms in 26 (78.8%). The sensitivity of SC was higher than that of SMF (78.8% versus 30.3%;P< 0.001).

Step generator with rise time ≦ 150 picosec

1965 ◽  
Vol 32 (1) ◽  
pp. 177
Author(s):  
J. Oleson ◽  
P.W. Keaton
Keyword(s):  
Rise Time ◽  
Step Generator

Concentration of Staphylococcal Enterotoxin from Food Extract Using Copper Chelate Sepharose

1989 ◽  
Vol 52 (12) ◽  
pp. 903-905 ◽  
Author(s):  
N. DICKIE ◽  
M. AKHTAR
Keyword(s):  
Rapid Method ◽  
Food Poisoning ◽  
Latex Agglutination ◽  
Staphylococcal Enterotoxin ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Partial Purification ◽  
Copper Chelate ◽  
Food Extract ◽  
Concentration Step

A simple and rapid method is described for detecting as little as 1 ng each of staphylococcal enterotoxins A, B, and C2 in 100 mL samples of food extracts. Samples were fractionated on copper chelate Sepharose 6B and the recovery of added staphylococcal enterotoxin was measured by the reversed passive latex agglutination test. An approximate 100 fold concentration of toxin was obtained. The method should prove useful as a partial purification and concentration step in the analysis of foods incriminated in food poisoning outbreaks.

A simple silver-staining technique for detecting Bence Jones proteins in unconcentrated urine.

1987 ◽  
Vol 33 (4) ◽  
pp. 561-563 ◽  
Author(s):  
J M Sheat ◽  
M A Lorier
Keyword(s):  
Silver Staining ◽  
Clinical Laboratory ◽  
Staining Technique ◽  
Silver Stain ◽  
Concentration Step ◽  
Silver Staining Technique ◽  
Bence Jones Proteins

Abstract Detection of Bence Jones proteins in urine usually involves a concentration step, followed by electrophoresis and, if necessary, immunofixation. The time-consuming and expensive concentration step can be eliminated by use of the silver-stain technique described here. This procedure, routinely used for staining unconcentrated urine, is inexpensive, sensitive, and easily performed in a clinical laboratory. Bence Jones proteins can be detected in concentrations as low as 5 mg/L.

Recovery of feline calicivirus infectious particles and genome from water: comparison of two concentration techniques

2003 ◽  
Vol 47 (3) ◽  
pp. 97-101 ◽  
Author(s):  
B. Gassilloud ◽  
M. Duval ◽  
L. Schwartzbrod ◽  
C. Gantzer
Keyword(s):  
Recovery Rate ◽  
Membrane Filtration ◽  
Glass Wool ◽  
Feline Calicivirus ◽  
Concentration Step ◽  
Filtration Technique

The aim of this work was to determine the recovery rate of feline calicivirus (FCV-F9) infectious particles and genome from water after a concentration step using either adsorption elution on glass wool or filtration through an electropositive membrane. The results showed that the membrane filtration technique allowed a 75% recovery rate of FCV-F9 infectious particles while the yield was only 5.3% for FCV-F9 genome. Using the glass wool adsorption-elution technique, the recovery rate of FCV-F9 infectious particles was 0.5% while the yield was 102.5% with Poliovirus 1.

scholarly journals Liquid-Liquid Extraction and Chromatography Process Routes for the Purification of Lithium

2019 ◽  
Vol 959 ◽  
pp. 79-99 ◽  
Author(s):  
Axel Schmidt ◽  
Fabian Mestmäcker ◽  
Lisa Brückner ◽  
Tobias Elwert ◽  
Jochen Strube
Keyword(s):  
Lithium Ion Batteries ◽  
Chemical Properties ◽  
Lithium Ion ◽  
High Growth ◽  
Liquid Extraction ◽  
Liquid Liquid Extraction ◽  
Production Processes ◽  
Concentration Step ◽  
Industrial Use ◽  
Increasing Demand

Since several years, the lithium market is characterized by high growth rates especially due to the increasing demand for lithium-ion batteries. Therefore, the primary production is currently expanded and there is a growing interest in recycling. However, because of the chemical properties of lithium, many production processes lack efficient processes for the separation, concentration and purification of lithium. This article reviews the current use of liquid-liquid extraction (LLE) and chromatography in lithium production as well as research. Currently, the industrial application of LLE and chromatography in lithium purification is limited to the extraction of impurities and co-products. Extraction of lithium is only used as concentration step in few processes before lithium precipitation. In research and development, a wide variety of extractants and resins is investigated. In LLE, chelating extractants like crown ethers and calixarene and synergistic systems show the greatest potential. In the chromatographic separation the main focus of research lies upon cation exchange media, especially media with sulfonated ligands. However, most research is still in early development. Therefore, extensive research is needed to enable the industrial use of optimized LLE and chromatography processes in lithium production. Content TOC \o "1-3" \h \z \u HYPERLINK \l "_Toc515547308" Abstract PAGEREF _Toc515547308 \h 2 HYPERLINK \l "_Toc515547309" Content PAGEREF _Toc515547309 \h 3

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