scholarly journals 1978 Field Studies of the Pink Bacterial Masses Which Grow at 90° C in the Outflow Channel of some Yellowstone Hot Springs

1978 ◽  
Vol 2 ◽  
pp. 135-140
Author(s):  
Robert Ramaley
Keyword(s):  
Fatty Acid Composition ◽  
National Park ◽  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Field Studies ◽  
Laboratory Culture ◽  
Unpublished Work ◽  
Biochemical Studies ◽  
Outflow Channel

The presence of masses of pink bacteria growing at 90° in the outflow channel in some hot springs in Yellowstone National Park was observed at least as early as 1899 (unpublished work of W. A. Setchell, cited in Brock, 1978, pp. 47-49). When Brock began his extensive microbiological studies of Yellowstone thermal areas in 1965, he also found "pink, gelatinous, stringy bacterial masses" growing at 90°C in the upper end of the runoff channel of Octopus Spring and published phase micrographs of these bacterial masses (Brock, 1978, pp. 45-46). However, Brock and his associates were never able to grow these bacteria in laboratory culture and beyond the determination of the fatty acid composition of the bacterial masses by Bauman and Simmond (1969) no other biochemical studies have been conducted with these extremely thermophilic bacteria.

scholarly journals Characterizing and Evaluating the Diverse Microbial Communities and Their Mercury Resistance Potential from Hot Spring Sites Representing Gradients in Temperature and pH in Yellowstone National Park

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Yelizaveta Rassadkina ◽  
Spencer Roth ◽  
Tamar Barkay
Keyword(s):  
Microbial Communities ◽  
Yellowstone National Park ◽  
National Park ◽  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Sequence Data ◽  
Mercury Resistance ◽  
Sample Site ◽  
Microorganism Community

Yellowstone National Park is home to many different hot springs, lakes, geysers, pools, and basins that range in pH, chemical composition, and temperature. These different environmental variations provide a broad range of conditions that select and grow diverse communities of microorganisms. In this study, we collected samples from geochemically diverse lakes and springs to characterize the microbial communities present through 16S rRNA metagenomic analysis. This information was then used to observe how various microorganisms survive in high mercury environments. The results show the presence of microorganisms that have been studied in previous literature. The results also depict gradients of microorganisms including thermophilic bacteria and archaea that exist in these extreme environments. In addition, beta diversity analyses of the sequence data showed site clustering based primarily on temperature instead of pH or sample site, suggesting that while pH, temperature, and sample site were all shown to be significant, temperature is the strongest factor driving microorganism community development. While it is important to characterize the microorganism community present, it is also important to understand how this community functions as a result of its selection. Along with looking at community composition, genomic material was tested to see if it contained mercury methylating (hgcA) or mercury reducing (merA) genes. Out of 22 samples, three of them were observed to have merA genes, while no samples had hgcA genes. These results indicate that microorganisms in Mustard and Nymph Springs may use mercury reduction. Understanding how microorganisms survive in environments with high concentrations of toxic pollutants is crucial because it can be used as a model to better understand mechanisms of resistance and the biogeochemical cycle, as well as for bioremediation and other solutions to anthropogenic problems.

scholarly journals Thermophilic and Pathogenic Amoebae From Hot Springs in Wyoming

1985 ◽  
Vol 9 ◽  
pp. 92-95
Author(s):  
William O'Dell ◽  
Robert Ramaley
Keyword(s):  
Long Range ◽  
Human Activity ◽  
Small Mammals ◽  
National Park ◽  
Hot Springs ◽  
Natural Sources ◽  
Grand Teton National Park ◽  
Cooperative Project ◽  
Reliable Methods

The long range goal of this research is to document the occurrence of pathogenic amoebae in thermal habitats that have been altered or disturbed by human activity. Immediate goals for this year included the development of reliable methods of detection for use in the field, the determination of the incidence and prevalence of thermophilic Naegleria in selected hot springs of Wyoming, and the investigation of potential natural sources of Naegleria. In addition, we initiated a cooperative project with Dr. A. J. Pinter to document the endoparasites of small mammals of the Grand Teton National Park.

scholarly journals Screening and characterization of thermo-active enzymes of biotechnological interest produced by thermophilic Bacillus isolated from hot springs in Tunisia

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Wajdi Thebti ◽  
Yosra Riahi ◽  
Rawand Gharsalli ◽  
Omrane Belhadj
Keyword(s):  
Thermal Water ◽  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Effect Of Temperature ◽  
Thermostable Enzymes ◽  
Thermophilic Bacillus ◽  
Enzyme Substrates ◽  
First Time

As part of the contribution to the global efforts in research of thermostable enzymes being of industrial interest, we focus on the isolation of thermophilic bacteria from Tunisian hot springs. Among the collection of 161 strains of thermophilic Bacillus isolated from different samples of thermal water in Tunisia, 20% are capable of growing at 100°C and the rest grow at 70°C or above. Preliminary activity tests on media supplemented with enzyme-substrates confirmed that 35 strains produced amylases, 37 - proteases, 43 - cellulases, 31 - xylanases and 37 - mannanases. The study of the effect of temperature on enzyme activity led to determination of the optimal temperatures of activities that vary between 60 and 100°C. Several enzymes were active at high temperatures (80, 90 and 100°C) and kept their activity even at 110°C. Several isolated strains producing enzymes with high optimal temperatures of activity were described for the first time in this study. Both strains B62 and B120 are producers of amylase, protease, cellulase, xylanase, and mannanase. The sequencing of 16S DNA identified isolated strains as Geobacillus kaustophillus, Aeribacillus pallidus, Geobacillus galactosidasus and Geobacillus toebii.

scholarly journals Biochemical Ecology of Extremely Thermophilic Bacteria 1979 Field Study in Yellowstone National Park

1979 ◽  
Vol 3 ◽  
pp. 116-120
Author(s):  
Robert Ramaley ◽  
Lily Jones
Keyword(s):  
Field Study ◽  
Yellowstone National Park ◽  
National Park ◽  
Biochemical Analysis ◽  
Bacterial Culture ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Field Studies ◽  
Primary Objective ◽  
Spring Runoff

The primary objective of the brief (one week) 1979 summer field studies conducted in Yellowstone National Park was to collect samples of the pink filamentous bacterial masses growing at 80-90° C in two Yellowstone hot spring runoff channels (Octopus Spring and Artist's Paint-pots) for subsequent biochemical analysis. In addition, samples were also taken from the runoff channels for bacterial culture and isolation of extremely thermophilic bacteria (including actinomycetes) and some additional Yellowstone thermal areas were also surveyed for the presence of the pink filamentous bacterial masses.

Orientation of processes of biological nitrogen transformation in winter rye agroecosystems under different levels of fertilization background

2016 ◽  
Vol 3 (3) ◽  
pp. 28-34
Author(s):  
V. Volkogon ◽  
I. Korotka
Keyword(s):  
Root Zone ◽  
Field Studies ◽  
Nitrogen Transformation ◽  
Mineral Nitrogen ◽  
Nitrogen Fertilizers ◽  
Winter Rye ◽  
Podzolic Soils ◽  
Microbial Preparation ◽  
Biological Nitrogen

Aim. To determine physiologically expedient rates of mineral nitrogen in winter rye production on sod-podzol- ic soils based on the orientation of the processes of biological nitrogen transformation in the plants rhizosphere. Methods. Field studies, gas chromatography determination of potential nitrogen fi xation activity and potential emissions of N 2 O. Results. The results obtained have demonstrated that the rates of mineral nitrogen, not ex- ceeding 60 kg/ha, can be considered physiologically expedient for winter rye production on sod-podzolic soils. Under the application of microbial preparation Diazobakteryn, there is a higher physiological need of plants for nitrogen, which allows increasing the rates of nitrogen fertilizers up to 90 kg/ha. Conclusions. The orienta- tion of the processes of biological nitrogen transformation in the root zone of plants is a reliable indicator of determining the appropriateness of nitrogen fertilization of crops.

The waters of Hot Springs National Park, Arkansas : their origin, nature and management

1974 ◽  
Author(s):  
M.S. Bedinger ◽  
F.J. Pearson ◽  
J.E. Reed ◽  
R.T. Sniegocki ◽  
C.G. Stone
Keyword(s):  
National Park ◽  
Hot Springs
Sign in / Sign up

Export Citation Format

Share Document