scholarly journals Leucine Aminopeptidase from Arachis hypogaea L. Seeds Partial Purification and Characterization

2017 ◽  
Vol 33 (5) ◽  
pp. 2676-2680 ◽  
Author(s):  
Taghreed U. Mohammd ◽  
Layla O. Farhan ◽  
Ashgan S. Dawood ◽  
Bushra F. Hasan
Keyword(s):  
Arachis Hypogaea ◽  
Leucine Aminopeptidase ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Arachis Hypogaea L

Partial purification and properties of apoplastic β-1,3 glucanases of groundnut leaves treated with glucan isolated from a biocontrol agent, Acremonium obclavatum

2000 ◽  
Vol 78 (2) ◽  
pp. 168-174 ◽  
Author(s):  
M Sathiyabama ◽  
R Balasubramanian
Keyword(s):  
Arachis Hypogaea ◽  
Partial Purification ◽  
Ph Optimum ◽  
Native Page ◽  
Puccinia Arachidis ◽  
Ammonium Sulphate Precipitation ◽  
Arachis Hypogaea L ◽  
Purification And Properties ◽  
Molecular Masses ◽  
Electrophoretic Techniques

Apoplastic β-1,3 glucanases (G1, G2) of Arachis hypogaea L. (peanut) leaves treated with glucan have been partially purified by ammonium sulphate precipitation, sephadex G-100, CM-Sephadex, DEAE-Sephadex chromatography, and preparative native PAGE electrophoretic techniques. The pI values of purified enzymes G1 and G2 were near 8 and 4, respectively. The apparent molecular masses of purified glucanases G1 and G2 from glucan-treated peanut leaves were 36 and 34 kDa, respectively. Both isoforms (G1 and G2) showed their pH optimum of 5.0 and temperature optimum of 40°C. The partially purified enzymes hydrolysed laminarin better than other substrates and inhibited uredospore germination of Puccinia arachidis. Both isoforms (G1 and G2) inhibited spore germination of some biocontrol agents such as Acremonium obclavatum W. Gams, Myrothecium verrucaria (Alb. Schw.) Ditmer, Fusarium solani (Mart.) Sacc., and Trichoderma harzianum Rifai.Key words: Acremonium obclavatum, Arachis hypogaea, β-1,3 glucanase, glucan, inhibition, Puccinia arachidis.

PURIFICATION AND CHARACTERIZATION OF A CHITINASE FROM PEANUT (ARACHIS HYPOGAEA L.)

2008 ◽  
Vol 32 (1) ◽  
pp. 32-45 ◽  
Author(s):  
SHAOYUN WANG ◽  
BIAO SHAO ◽  
XIUYUN YE ◽  
PINGFRAN RAO
Keyword(s):  
Arachis Hypogaea ◽  
Purification And Characterization ◽  
Arachis Hypogaea L

scholarly journals Extraction, Characterization and Partial Purification of L-Asparaginase from the Leaves of Arachis Hypogaea L.

2019 ◽  
Vol 16 (3) ◽  
pp. 681-691
Author(s):  
Niranjana. J Niranjana ◽  
Kandasamy Arun Gandhi ◽  
D. Sunmathi ◽  
P. Nanthavanan
Keyword(s):  
Metal Ions ◽  
Arachis Hypogaea ◽  
Specific Activity ◽  
Lymphoblastic Leukemia ◽  
Gel Filtration ◽  
Size Exclusion ◽  
Partial Purification ◽  
Crude Enzyme Extract ◽  
Ammonium Sulphate Precipitation ◽  
Arachis Hypogaea L

L-asparaginase has been a promising therapeutic agent in the treatment of acute lymphoblastic leukemia and lymphoma. In recent times, due to the side effects of commercially available bacterial L-asparaginase and its unavoidable importance, plants are being explored as the source of L-asparaginase. The enzyme L-asparaginase was partially purified from Arachis hypogaea L. The crude enzyme extract was subjected to different purification steps including ammonium sulphate precipitation, dialysis followed by separation on Sephadex G-100 gel filtration (size exclusion chromatography) to obtain partially pure form of L - asparaginase. The enzyme was partially purified to 118 folds and contained specific activity of 4686.86 U/mg with 9.85% yield. SDS-PAGE electrophoresis of the partially purified enzyme revealed that it was a single protein with molecular weight of 70 kDa. The study on physiochemical properties showed that L - asparaginase from Arachis hypogaea L. was potassium-dependent in nature, where its optimum pH of enzyme activity was found to be 8.0 and temperature as 40°/50°C with reaction time of 15 - 20 minutes. Also it was observed that the L-asparaginase activity increased with the presence of metal ions such as Na+, Mg++, making it an enzyme dependent on metal ions for its reaction. In addition to this, it was revealed that the enzyme was partially inhibited in presence of certain chelators. The specificity of L-asparaginase obtained from Arachis hypogaea L. with lack of urease activity and minimal glutaminase activity along with less cytotoxicity on human blood indicated it as an efficient chemotherapeutic agent that could be investigated further in future studies.

GERMINACIÓN DE SEMILLAS DE CACAHUATE (Arachis hypogaea L.) UTILIZANDO DIFERENTES DOSIS DE GIBERELINA.

2018 ◽  
Vol 11 (11) ◽  
Author(s):  
S.A. García Muñoz
Keyword(s):  
Arachis Hypogaea ◽  
Arachis Hypogaea L

Objetivo: Evaluar la germinación de cacahuate (Arachis hypogaea L.) mediante el uso de diferentes dosis de ácido giberélico (GA3). Diseño/metodología/aproximación: Se empleó un diseño completamente al azar. Se utilizaron tres tratamientos con 20 repeticiones. Tratamiento 1: 0.05gr/L de ácido giberélico (GA3), Tratamiento 2: 0.10gr/L de ácido giberélico (GA3), Tratamiento 3: 0.15gr/L de ácido giberélico (GA3) y Tratamiento 0: Testigo. Se utilizaron semillas de cacahuate de la variedad Virginia. Los parámetros a evaluar fueron, la altura de plántula, número de hojas, medida de raíz y biomasa.  Las medias fueron comparadas por la prueba de Tukey a un nivel del 5% de confianza. Resultados: Los tratamientos indicaron que el Tratamiento 0 (Testigo) obtuvo un porcentaje de germinación de 85%, siendo mayor que el tratamiento 3 (0.15gr/L de GA3) con un 75% de germinación, sin embargo, el tratamiento 1 (0.05gr/L de GA3) y 2 (0.10gr/L de GA3) presentaron una mejor respuesta al obtener un 95% de germinación cada uno. Limitaciones del estudio/implicaciones: El tratamiento 3 causa efectos negativos en la germinación de la planta. Hallazgos/conclusiones: Es necesario dar seguimiento a la investigación para un mejor control del ambiente y ampliar las dosis de GA3, así como aumentar la velocidad de germinación aplicando 0.15gr/L de GA3.

Direct in vitro Regeneration of Arachis Hypogaea L.(Jl-24) and Study of Salt Tolerance

2011 ◽  
Vol 3 (8) ◽  
pp. 52-54 ◽  
Author(s):  
Bhagya Lakshmi Jyothi Kusuma ◽  
◽  
Sharmila Begum S
Keyword(s):  
Salt Tolerance ◽  
Arachis Hypogaea ◽  
In Vitro Regeneration ◽  
Arachis Hypogaea L

QUALIDADE FISIOLÓGICA E SANITÁRIA DE SEMENTES DE AMENDOIM (Arachis hypogaea L.) ARMAZENADAS

2001 ◽  
Vol 3 (1) ◽  
pp. 61-72 ◽  
Author(s):  
E. Diniz ◽  
C.L. Silva ◽  
M.B. Muniz ◽  
V.P. Queiroga ◽  
R.L.A. Bruno
Keyword(s):  
Arachis Hypogaea ◽  
Arachis Hypogaea L

Respon Pertumbuhan dan Produksi Kacang Tanah (Arachis hypogaea L.) terhadap Sistem Tanam Alur dan Pemberian Jenis Pupuk

2017 ◽  
Vol 5 (3) ◽  
pp. 342
Author(s):  
Rizal Mahdi Kurniawan ◽  
Heni Purnamawati ◽  
Yudiwanti Wahyu E. K
Keyword(s):  
Arachis Hypogaea ◽  
Arachis Hypogaea L ◽  
Split Plot ◽  
Plot Design

<p><em>Penelitian ini bertujuan untuk mempelajari pengaruh sistem tanam alur dan pemberian jenis pupuk terhadap pertumbuhan dan produksi kacang tanah (Arachis hypogaea L.). Penelitian dilaksanakan di KP Leuwikopo IPB Dramaga, Bogor pada bulan Februari - Juni 2013. Percobaan menggunakan rancangan petak terbagi (Split Plot Design) dengan perlakuan terdiri dari dua faktor, yaitu sistem tanam alur sebagai petak utama dan jenis pupuk sebagai anak petak. Hasil penelitian menunjukkan bahwa perlakuan sistem tanam alur meningkatkan daya hasil pada produktivitas biji kering, produktivitas polong kering, dan bobot kering biji per tanaman lebih baik dibandingkan sistem tanam konvensional. Hal tersebut ditunjukkan dengan perlakuan sistem tanam alur yang memiliki produktivitas 2.93 ton/ha polong kering, sedangkan sistem tanam konvensional sebesar 2.55 ton/ha polong kering. Sistem budidaya kacang tanah pada sistem tanam alur dapat meningkatkan efisiensi tanaman dalam memanfaatkan unsur hara yang telah diberikan baik pupuk organik maupun anorganik, sehingga pertumbuhan dan hasil tanaman kacang tanah menjadi lebih baik. Pemberian jenis pupuk kandang ayam + Dolomit + NPK memberikan respon terhadap pertumbuhan dan  daya hasil rata-rata tanaman yang lebih baik dibandingkan jenis pupuk lainnya.</em></p>

Comparison of foliar and soil applications for correction of iron deficiency in peanut (Arachis hypogaea L.)

2019 ◽  
Vol 51 (3) ◽  
Author(s):  
Shamim Akhtar ◽  
Nazneen Bangash ◽  
Muhammad Sajjad Iqbal ◽  
Armghan Shahzad ◽  
Muhammad Arshad ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Arachis Hypogaea ◽  
Arachis Hypogaea L
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