A portable and automatic biosensing instrument for simultaneous detection of foodborne pathogens using nanobead-based magnetic separation and quantum dot-labeled fluorescent measurement

2016 ◽  
Keyword(s):  
Quantum Dot ◽  
Magnetic Separation ◽  
Foodborne Pathogens ◽  
Simultaneous Detection

scholarly journals Development of a multicolor upconversion fluorescence immunoassay for the simultaneous detection of thiamethoxam and dextran by magnetic separation

RSC Advances ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 517-524
Author(s):  
Chuanyong Li ◽  
Wanlin Sun ◽  
Lianrun Huang ◽  
Nana Sun ◽  
Xiude Hua ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Determination ◽  
Magnetic Separation ◽  
Simultaneous Detection ◽  
Fluorescence Immunoassay ◽  
Upconversion Fluorescence

The anti-thiamethoxam and anti-dextran monoclonal antibodies were prepared to develop a multicolor upconversion fluorescence immunoassay for the simultaneous determination of thiamethoxam (544 nm) and dextran (477 nm).

scholarly journals Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads

2020 ◽  
Vol 92 (23) ◽  
pp. 15542-15549
Author(s):  
Chongwen Wang ◽  
Xingsheng Yang ◽  
Bing Gu ◽  
Haifeng Liu ◽  
Zihui Zhou ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Dual Mode

Developing a multiplex real-time PCR with a new pre-enrichment to simultaneously detect four foodborne bacteria in milk

2019 ◽  
Vol 14 (10) ◽  
pp. 885-898 ◽  
Author(s):  
Moezi Parichehr ◽  
Kargar Mohammad ◽  
Doosti Abbas ◽  
Khoshneviszadeh Mehdi
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Reliable Technique ◽  
E Coli ◽  
Foodborne Bacteria ◽  
Multiple Pathogens ◽  
Enrichment Step

Aim: The aim of this study is to formulate a new single nonselective pre-enrichment medium (ELSS) that can support the concurrent growth of four major foodborne pathogens containing E. coli O157: H7, L. monocytogenes, S. aureus and S. enterica serovar Entertidis to develop a multiplex TaqMan Real-time PCR (mRT-PCR). Methods: The mRT-PCR with a new pre-enrichment was carried out for simultaneous detection and quantification of these foodborne bacteria. Results: By using mRT-PCR after 16 h pre-enrichment in ELSS, the detection limit of each pathogen was 1 CFU/25 ml contaminated milk, as well as inclusivity and exclusivity reached 100%. Conclusion: The mRT-PCR assay with pre-enrichment step is a fast and reliable technique for detecting single or multiple pathogens in food products.

scholarly journals Integration of isothermal amplification with quantum dot-based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs

2018 ◽  
Vol 9 (18) ◽  
pp. 4258-4267 ◽  
Author(s):  
Juan Hu ◽  
Ming-hao Liu ◽  
Chun-yang Zhang
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Fluorescence Resonance Energy Transfer ◽  
Rolling Circle Amplification ◽  
Simultaneous Detection ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Isothermal Amplification ◽  
Rolling Circle ◽  
Fluorescence Resonance

The integration of quantum dot-based fluorescence resonance energy transfer with rolling circle amplification enables simultaneous sensitive detection of multiple microRNAs.

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