Inhibitory Effects of GGX on Lung Injury of Chronic Obstructive Lung Disease (COPD) Mice Model

Objectives: This study is aimed to evaluate the protective effects of GGX on lung injury of Chronic Obstructive Lung Disease (COPD) mice model. Materials and Methods: C57BL/6 mice were challenged with lipopolysaccharide (LPS) and cigarette smoke extract (CSE) and then treated with vehicle only (Control group), dexamethasone 3 ㎎/㎏ (Dexa group), gam-gil-tang 200 ㎎/㎏ (GGT group), GGX 100, 200, and 400 ㎎/㎏ (GGX group). After sacrifice, its bronchoalveolar lavage fluid (BALF) or lung tissue was analyzed with cytospin, Enzyme-Linked Immunosorbent Assay (ELISA), real-time polymerase chain reaction (PCR) and hematoxylin & eosin (H&E), and Masson’s trichrome staining. Results: In the COPD model, GGX significantly inhibited the increase of neutrophils, TNF-α, IL-17A, CXCL-1, MIP2 in BALF and TNF-α, IL-1β, IL-10 mRNA expression in lung tissue. It also decreased the severity of histological lung injury. Conclusion: This study suggests the usability of GGX for COPD patients by controlling lung tissue injury.

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Carnitine deficiency in chronic obstructive lung disease: A study in a tertiary care centre

IP Indian Journal of Immunology and Respiratory Medicine ◽

10.18231/j.ijirm.2021.040 ◽

2021 ◽

Vol 6 (3) ◽

pp. 183-187

Author(s):

M Ravindranath ◽

C H Raju

Keyword(s):

Lung Disease ◽

Obstructive Lung Disease ◽

Tertiary Care ◽

Chronic Obstructive Lung Disease ◽

Carnitine Deficiency ◽

Control Group ◽

Chronic Obstructive ◽

X Rays ◽

Tertiary Care Centre ◽

Regular Diet

Chronic Obstructive Lung Disease (COPD) is a condition where there is poor airflow to the lungs and over a period of time this condition worsen, resulting in severe morbidity. L-carnitine (LC) is a soluble qaurtenary amine and is essential for the tissues and lung tone to function normally. This study was therefore find out the association of L-carnitine in patients with chronic Obstructive Pulmonary Disease. 75 patients who came to the Department of Pulmonology of either sex between 40 to 75 years with confirmed COPD were included into patients group and 75 healthy patients were included into the control group. Blood was collected for Fasting blood sugar, AST, ALP, ALT, Urea, Creatinine, Albumin, pre-albumin, iron , magnesium, sodium, phosphorous, calcium, Acylcarnitines, total and free carnitine levels. X-rays were taken and pulmonary function test was done for all the patients. The mean age of the patients in our study was 62.53 and 61.86 years among the patients and controls respectively. Among the controls the Ph and Fe levels were 3.1 ± 0.9mmol/L and 21.69 ± 3.19 mcg/dL respectively and among the patients the same levels were 2.6 ± 1.9mmol/L and 43.82 ± 8.22mcg/dL respectively. The albumin and the prealbumin in the patients were significantly lower in the patients with 21.81 ± 2.88 and 23.53 ±2.58 mg/dL and in controls they were 32.61 ±3.87 and 26.87 ± 1.93 mg/dL respectively. There was a considerable carnitine deficiencies in COPD patients when compared to the controls and since carnitine can be found in regular diet, people can be educated to have a proper intake of foods containing carnitine in their regular diet.

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Muscarinic Cholinergic Receptors in Peripheral Lung Tissue of Normal Subjects and of Patients with Chronic Obstructive Lung Disease

Clinical Science ◽

10.1042/cs0660585 ◽

1984 ◽

Vol 66 (5) ◽

pp. 585-590 ◽

Cited By ~ 19

Author(s):

J. A. M. Raaijmakers ◽

G. K. Terpstra ◽

A. J. Van Rozen ◽

A. Witter ◽

J. Kreukniet

Keyword(s):

Lung Disease ◽

Lung Tissue ◽

Binding Sites ◽

Obstructive Lung Disease ◽

Chronic Obstructive Lung Disease ◽

Cholinergic Receptors ◽

Chronic Obstructive ◽

Muscarinic Cholinergic Receptors ◽

Peripheral Lung ◽

Muscarinic Cholinergic

1. Muscarinic cholinergic receptors have been identified and characterized by radioligand binding studies in human peripheral lung tissue. The tissue was obtained at thoracotomy of 12 patients, of whom four had chronic obstructive lung disease. 2. The radioligand l-quinuclidinyl[phenyl-4-3H]benzilate (3H-QNB) was used to label the muscarinic cholinergic receptors. Binding was saturable, protein dependent and showed a high affinity and stereospecificity. Specific binding could be inhibited by agonists and antagonists; molar inhibition constants determined for the agents used were of the same order of magnitude as those reported for 3H-QNB inhibition in various tissues of laboratory animals. Inhibition experiments with agonists resulted in Hill slopes which were significantly different from unity, indicating multiple binding sites. The stable GTP analogue guanyl-5′-imidodiphosphate had no effect on the Hill slopes of agonists or antagonists. 3. The number of binding sites was significantly less in lung tissue from patients with chronic obstructive lung disease.

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Diagnostic Role of Osteopontin in Interstitial Lung Disease

Journal of Health Science and Medical Research ◽

10.31584/jhsmr.2021780 ◽

2021 ◽

Author(s):

Meena Goyal ◽

Shivani Jaswal ◽

Kranti Garg ◽

Seema Gupta ◽

Soma Dey ◽

...

Keyword(s):

Interstitial Lung Disease ◽

Lung Disease ◽

Obstructive Lung Disease ◽

Chronic Obstructive Lung Disease ◽

Future Research ◽

Enzyme Linked Immunosorbent Assay ◽

Chronic Obstructive ◽

P Value ◽

Treatment Protocols ◽

The Difference

Objective: Early diagnosis of interstitial lung disease (ILD) is desirable not only to better define pathobiological mechanisms, but also to customize treatment protocols. As osteopontin (OPN) expression may be linked to the fibrotic remodelling in ILD, this present study was planned to evaluate the usefulness of its estimation in serum for the diagnosis of ILD.Material and Methods: Serum OPN levels were estimated by enzyme-linked immunosorbent assay in 52 diagnosed cases of ILD, that were then compared with 46 patients with chronic obstructive lung disease and 46 apparently healthy controls.Results: Median levels of serum OPN were found to be significantly higher in patients with ILD, 2.186 nanograms per milliliter (ng/ml), as compared to patients with chronic obstructive lung disease and healthy controls (1.687 and 1.923 ng/ml, respectively). The best cutoff levels of OPN, for diagnosis of ILD, was found to be >1.08 ng/ml; with a sensitivity of 88.4% and specificity of 32.6%. Of the various subtypes, serum OPN levels were found to be highest in patients with idiopathic pulmonary fibrosis and interstitial pneumonia with autoimmune features; although the difference in levels between various subgroups was not found to be statistically significant (p-value=0.495).Conclusion: Serum concentration of OPN was found to be increased in patients with ILD and may be used to aid diagnosis. This opens new avenues for future research in patients with ILD, not only for the validation of the diagnostic abilities of OPN, but also as a potential target in its treatment.

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