scholarly journals Improving SCOBY starter using co-culture of tapai and bakery yeast

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
Aurora Urbahillah ◽  
Jay Jayus ◽  
NURHAYATI NURHAYATI
Keyword(s):  
Baker’S Yeast ◽  
Candida Krusei ◽  
Baker's Yeast ◽  
Oval Cell ◽  
The Third ◽  
Trichoderma Sp ◽  
Candida Famata ◽  
Fermentation Profile ◽  
Fusarium Sp ◽  
Bakery Yeast

Abstract. Urbahillah A, Jayus J, Nurhayati N. 2021. Improving SCOBY starter using co-culture of tapai and bakery yeast. Biodiversitas 22: 4617-4624. Kombucha is a beverage fermented by a symbiotic bacteria and yeast known as SCOBY (Symbiotic Culture of Bacteria and Yeast). Bacteria and yeast contribute to the formation of organic acids, aroma, taste, and flavor of kombucha. The commercial yeasts used in Indonesian are baker’s yeast and tapai yeast. This study was conducted to develop SCOBYco-culture with tapai yeast and baker’s yeast and evaluate its activity. The ingredients for the kombucha were cascara, water, and sugar, which were fermented with three formula starter, i.e. original SCOBY 10% w/v (SN), co-culture SCOBY 10% w/v with 0.1% w/v of baker’s yeast (SNR), and co-culture SCOBY 10% w/v with tapai yeast 0.1% w/v (SNT). The starter activity were determined based on the OD (Optical Density) value. Yeast screening was carried out on the dominant starter population. Furthermore, morphologically yeast was identified based on colony type, color, and shape of cell. Then yeast was identified by their fermentation profile using API 20C Aux Kit. Isolate A showed white colony with convex elevation and the cell was round-shaped. Colony of isolate B and isolate C were creamy in color and oval cell shaped. The API results revealed that the first isolate was identified as Candida famata, second isolate was as Candida krusei, and the third isolate was as Candida magnoliae. Three types of fungi were found from SCOBY, namely Mucor sp., Trichoderma sp., and Fusarium sp. Mucor sp. has non-septate hyphae, and round black spores. Trichoderma sp. has septate hyphae, greenish-white spores, and the conidia have the shape of globose to ellipsoidal Fusarium sp. has a mold with septate hyphae, yellowish-white colonies, and the conidia have the shape of obovoid. Bacteria, yeast, and mold present in the medium form a powerful symbiosis for produce metabolite.

Candida krusei in Baker's yeast production

1981 ◽  
Vol 13 (2) ◽  
pp. 113-116 ◽  
Author(s):  
Maija-Liisa Suihko ◽  
Veijo Mäkinen
Keyword(s):  
Baker’S Yeast ◽  
Candida Krusei ◽  
Baker's Yeast ◽  
Yeast Production

scholarly journals Imprint and spreading techniques for the isolation and identification of subungueal fungi in claws of healthy cats

2021 ◽  
Vol 15 (1) ◽  
pp. 30-35
Author(s):  
Camilla Freitas Oliveira ◽  
Jamille Bispo de Carvalho ◽  
Luiza Montenegro Cintra Castro ◽  
Paula Elisa Brandão Guedes ◽  
Katharine Costa dos Santos ◽  
...  
Keyword(s):  
Pathogenic Fungus ◽  
Brain Heart Infusion ◽  
Brain Heart Infusion Broth ◽  
Isolation And Identification ◽  
The Third ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Petri Dishes ◽  
Aspergillus Sp ◽  
Rhizopus Sp

The aim of this study was to compare imprint and spreading techniques for the isolation and identification of colonies of pathogenic and non-pathogenic fungus in the claws of semidomiciliated cats. For that propose, 150 cats were evaluated, subdivided into three groups of 50 animals. In the first and second groups, the cats were submitted to the imprint technique in Petri dishes containing Selective Mycobiotic Agar: In the first group, the cats were subjected underwent antisepsis with 70% ethanol of the claws of the thoracic limbs and in the second group the animals were subjected underwent antisepsis with 70% ethanol of the claws of only one of the thoracic limbs. The third group was submitted to the spreading technique, whose material was collected by rubbing a sterile swab moistened with brain-heart infusion broth, in the claws of the forelimbs, where an aliquot of the material was transferred to Petri dishes containing Selective Mycobiotic Agar. The material was stored at 25°C for 30 days. The readings were performed on days 5, 7, 15, and 30 post incubation. Using the imprinttechnique performed under the conditions of this experiment, we were not able to isolate and identify the colonies because since day 5, they were overlapped. From the spreading technique, Mucor sp. (54,34%), Rhodotorula sp. (28,26%), Fusarium sp. (21,73%), Aspergillus sp. (21,73%), Trichoderma sp. (19,56%), Penicillium sp. (19,56%), Cladosporium sp. (10,86%), Rhizopus sp. (8,68%), Acremonium sp. (6,5%), Exophialia sp. (6,5%), Paecilomyces sp. (4,34%), Trichosporon sp. (4,34%), and Geotrichum sp. (2,17%) were isolated. It was concluded that the spreading technique proved to be useful in isolating fungal colonies from feline claws, and the animals do not present symptoms, which signals the importance of them as possible sources of exposure for tutors. The cats were negative for Sporothrix sp. by the imprint and spreading techniques.

scholarly journals ECOLOGIZATION OF JOINT PRODUCTION OF ALCOHOL AND BAKERY YEAST FROM MOLASSES

2020 ◽  
Vol 36 (4) ◽  
pp. 74-82
Author(s):  
LEVANDOVSKY Leonid ◽  
VITRIAK Oksana ◽  
HRABOVSKA Olena
Keyword(s):  
Dry Matter ◽  
Raw Materials ◽  
Matter Content ◽  
Baker’S Yeast ◽  
Joint Production ◽  
Specific Yield ◽  
Dry Matter Content ◽  
Baker's Yeast ◽  
Product Technology ◽  
Bakery Yeast

Background. The disadvantage of the two-product technology of alcohol and baker’s yeast from molasses is the need to limit the concentration of dry matter of the source medium to 22% and, accordingly, the level of alcohol accumulation in mature malt not more than 8.0% vol. to prevent deterioration of baker’s yeast. The consequence of this is the high specific cost of steam for bragorectification and increase the specific yield of post-alcohol molasses bard in comparison with single-product technology (obtaining only alcohol). Materials and methods. Raw materials, intermediates and final products were analyzed according to the methods adopted in science and practice of molasses alcohol technology. The dry matter content; pH of the medium; the content of alcohol in the brew, yeast biomass and unfermented sugars; the lifting power of yeast and their color in the resulting products were determined. Results. The possibility of reducing the volume of post-alcoholic molasses bard by returning it for the preparation of molasses bard without deteriorating the quality of baker’s yeast and alcohol yield, as well as by increasing the dry matter concentration of fermented wort from 22 (traditional technology) to 27% and accumulation of alcohol in mature brew up to 10–11% vol. has been experimentally proven. Conclusion. The presented research results provide grounds for the development of energy-saving low-waste two-stage technology of alcohol and baking yeast from mo­lasses, which can be used to obtain high-quality baking yeast to increase the con­centration of alcohol in mature malt by more than 10% vol. This will significantly reduce the steam for bragorectification of alcohol and the volume of contaminated and difficult to dispose of production waste – post-alcohol molasses bard. The implementation of such technology will be the next step in the greening of the joint production of alcohol and baker’s yeast from molasses.

COD REDUCTION OF BAKER'S YEAST WASTEWATER USING BATCH ELECTROCOAGULATION

2014 ◽  
Vol 13 (12) ◽  
pp. 3153-3160 ◽  
Author(s):  
Zakaria Al-Qodah ◽  
Mohammad Al-Shannag ◽  
Kholoud Alananbeh ◽  
Nahla Bouqellah ◽  
Eman Assirey ◽  
...  
Keyword(s):  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Cod Reduction ◽  
Yeast Wastewater

The use of β-glucan extracted from baker’s yeast (Saccharomyces cerevisiae) to increase non-specific immune system and resistence of tilapia (Oreochromis niloticus) to Aeromonas hydrophila

2013 ◽  
pp. 92
Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immune System ◽  
Aeromonas Hydrophila ◽  
Phagocytic Activity ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Randomized Design ◽  
The Difference ◽  
Motile Aeromonas Septicaemia

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©

Improvement of cadaverine production in whole cell system with baker’s yeast for cofactor regeneration

2021 ◽  
Author(s):  
Yeong-Hoon Han ◽  
Hyun Joong Kim ◽  
Tae-Rim Choi ◽  
Hun-Suk Song ◽  
Sun Mi Lee ◽  
...  
Keyword(s):  
Cell System ◽  
Baker’S Yeast ◽  
Cofactor Regeneration ◽  
Baker's Yeast ◽  
Whole Cell

scholarly journals The preparation and characterization of highly purified, enzymically active complex III from baker's yeast.

1978 ◽  
Vol 253 (7) ◽  
pp. 2392-2399 ◽  
Author(s):  
J.N. Siedow ◽  
S. Power ◽  
F.F. de la Rosa ◽  
G. Palmer
Keyword(s):  
Baker’S Yeast ◽  
Complex Iii ◽  
Baker's Yeast ◽  
Active Complex
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