scholarly journals Imprint and spreading techniques for the isolation and identification of subungueal fungi in claws of healthy cats

2021 ◽  
Vol 15 (1) ◽  
pp. 30-35
Author(s):  
Camilla Freitas Oliveira ◽  
Jamille Bispo de Carvalho ◽  
Luiza Montenegro Cintra Castro ◽  
Paula Elisa Brandão Guedes ◽  
Katharine Costa dos Santos ◽  
...  
Keyword(s):  
Pathogenic Fungus ◽  
Brain Heart Infusion ◽  
Brain Heart Infusion Broth ◽  
Isolation And Identification ◽  
The Third ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Petri Dishes ◽  
Aspergillus Sp ◽  
Rhizopus Sp

The aim of this study was to compare imprint and spreading techniques for the isolation and identification of colonies of pathogenic and non-pathogenic fungus in the claws of semidomiciliated cats. For that propose, 150 cats were evaluated, subdivided into three groups of 50 animals. In the first and second groups, the cats were submitted to the imprint technique in Petri dishes containing Selective Mycobiotic Agar: In the first group, the cats were subjected underwent antisepsis with 70% ethanol of the claws of the thoracic limbs and in the second group the animals were subjected underwent antisepsis with 70% ethanol of the claws of only one of the thoracic limbs. The third group was submitted to the spreading technique, whose material was collected by rubbing a sterile swab moistened with brain-heart infusion broth, in the claws of the forelimbs, where an aliquot of the material was transferred to Petri dishes containing Selective Mycobiotic Agar. The material was stored at 25°C for 30 days. The readings were performed on days 5, 7, 15, and 30 post incubation. Using the imprinttechnique performed under the conditions of this experiment, we were not able to isolate and identify the colonies because since day 5, they were overlapped. From the spreading technique, Mucor sp. (54,34%), Rhodotorula sp. (28,26%), Fusarium sp. (21,73%), Aspergillus sp. (21,73%), Trichoderma sp. (19,56%), Penicillium sp. (19,56%), Cladosporium sp. (10,86%), Rhizopus sp. (8,68%), Acremonium sp. (6,5%), Exophialia sp. (6,5%), Paecilomyces sp. (4,34%), Trichosporon sp. (4,34%), and Geotrichum sp. (2,17%) were isolated. It was concluded that the spreading technique proved to be useful in isolating fungal colonies from feline claws, and the animals do not present symptoms, which signals the importance of them as possible sources of exposure for tutors. The cats were negative for Sporothrix sp. by the imprint and spreading techniques.

INVENTARISASI JAMUR PADA BENIH BEBERAPA VARIETAS KEDELAI [Glycine max (L.) Merril] YANG BERADA DI SEKITAR BANDAR LAMPUNG

2014 ◽  
Vol 2 (3) ◽  
Author(s):  
Reny Destya Angrum ◽  
Muhammad Nurdin ◽  
Suskandini Ratih ◽  
Hasriadi Mat Akin
Keyword(s):  
Glycine Max ◽  
Agar Plate ◽  
Plate Test ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Glycine Max L ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

Penelitian ini bertujuan untuk menginventarisasi jamur yang terdapat pada beberapa varietas benih kedelai [ Glycine max (L.) Merril ] yang berada di sekitar Bandar Lampung. Penelitian ini dilakukan di Laboratorium Penyakit Tanaman, UniversitasLampung dari Mei 2013 sampai dengan Agustus 2013. Penelitian ini menggunakan kedelai varieta Burangrang, Kaba, Argomulyo, Tanggamus dan Grobogan. Benih-benih tersebut diinkubasi menggunakan metode blotter test dan agar plate test dengan dua perlakuan, yaitu kontrol (tanpa NaOCl) dan dengan menggunakan NaOCl. Jamur-jamur yang tumbuh dari benih yang diinkubasi diidentifikasi dengan cara diamati dan difoto, selanjutnya dicocokkan dengan bubu-buku referensi tentang penyakit- penyakit kedelai. Selain itu juga dilakukan penghitungan persentase kemunculan jamur dari benih yang diinkubasi. Hasil yang diperoleh dari penelitian yang dilakukan dengan perlakuan desinfeksi maupun tanpa desinfeksi ditemukan jenis jamur yang berasal dari lapang (Fusarium sp., Cercospora sp., Sclerotium sp., Phomopsis sp., Rhizoctonia sp., dan Colletotrichum sp.) dan jenis jamur yang berasal dari gudang penyimpanan (Trichoderma sp., Aspergillus sp., Penicillium sp., dan Rhizopus sp.). Benih yang diberikan perlakuan desinfeksi dapat mengurangi tumbuhnya jamur yang berasal dari gudang/tempat penyimpanan (kontaminan) tetapi tidak menghilangkan jenis jamur yang menginfeksi di dalam benih (jamur yang berasal dari lapang). Pada varietas Grobogan Rhizoctonia sp. memiliki persentase kemunculan jamur yang lebih tinggi dibandingkan dengan jamur lainnya yaitu sebesar 60,56%. Pada benih dengan perlakuan tanpa desinfeksi persentase kemunculan Aspergillus sp. pada varietas Kaba dan Tanggamus lebih tinggi dibandingkan dengan jamur lainnya sebesar 63,33%. Pada media perlakuan desinfeksi dengan menggunakan media kertas saring persentase kemunculan jamur pada varietas Kaba Cercospora sp. memiliki persentase lebih tinggi dibandingkan dengan jamur lainnya sebesar 30%. Pada media perlakuan tanpa desinfeksidengan menggunakan media kertas saring, Trichoderma sp. pada varietas Kaba memiliki persentase kemunculan jamur lebih tinggi dibandingkan dengan jamur lainnya, dengan persentase kemunculan masing-masing sebesar 77,23%.

scholarly journals Associação de Fungos com Sementes de Espécies Florestais Nativas

2017 ◽  
Vol 43 (3) ◽  
pp. 246-247 ◽  
Author(s):  
Ana Lídia Moura do Carmo ◽  
Edson José Mazaratto ◽  
Bárbara Eckstein ◽  
Álvaro Figueredo dos Santos
Keyword(s):  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

RESUMO No Brasil, ainda há poucas pesquisas sobre os microrganismos associados às sementes florestais. O objetivo desse trabalho foi detectar fungos associados às sementes de seis espécies florestais nativas: açoita-cavalo (Luehea divaricata), araçá amarelo (Psidium cattleyanum), corticeira (Erythrina falcata), rabo-de-bugio (Lonchocarpus sp.), vassoura-vermelha (Dodonea viscosa) e pau-jacaré (Piptadenia gonoacantha). Foram identificados oito gêneros de fungos potencialmente patogênicos: Cladosporyum sp., Fusarium sp., Pestalotiopsis sp., Phomopsis sp., Coletotrichum sp., Alternaria sp., Phoma sp. e Botrytis sp. e fungos saprófitas: Aspergillus sp., Penicillium sp., Rhizopus sp., Epicoccum sp., Torula sp., Trichoderma sp., Gliocladium sp., Chaetomium sp. e Trichothecium sp. Os resultados evidenciam a diversidade de fungos associados às sementes florestais nativas, incluindo vários potencialmente fitopatogênicos.

Yeasts and Molds Isolated from Spoiling Citrus Products and By-Products1

1989 ◽  
Vol 52 (4) ◽  
pp. 261-263 ◽  
Author(s):  
MICKEY E. PARISH ◽  
DIANNE P. HIGGINS
Keyword(s):  
Orange Juice ◽  
Aureobasidium Pullulans ◽  
Candida Maltosa ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Pichia Membranaefaciens ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Yeasts And Molds

A variety of citrus products, including dried peel used as livestock fodder, unpasteurized orange juice and pasteurized, and chilled orange juice were selectively surveyed for their fungal microflora. Fungi isolated were 1) pasteurized orange juice: Aureobasidium pullulans, Cladosporium sp., and Penicillium sp., 2) unpasteurized orange juice: Candida maltosa, C. sake, Fusarium sp., Geotrichum sp., Hanseniaspora sp., H. guilliermondii, Penicillium sp., Pichia membranaefaciens, Saccharomyces cerevisiae, Schwanniomyces occidentalis, and Torulaspora delbrueckii and 3) dried peel: Aspergillus niger, Aspergillus sp., Byssochlamys sp., Fusarium sp., Penicillium sp., Rhizopus sp., Rhodotorula sp., Sc. occidentalis, and Trichoderma sp.

scholarly journals Isolation frequency of medically important fungi and fluconazole resistant of Candida sp from hospital cockroaches.

2020 ◽  
Author(s):  
Doreen Anna Mloka ◽  
George M Bwire ◽  
Kenndy Mwambete
Keyword(s):  
Fungal Pathogens ◽  
Isolation And Identification ◽  
Opportunistic Fungi ◽  
Candida Sp ◽  
Microdilution Method ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Parasitic Pathogens

Abstract BackgroundCockroaches are common pests in homes and hospitals. They are known to cause allergic reactions in certain individuals and have found to be potential vectors for various bacterial and parasitic pathogens. This study assessed the potential of hospital cockroaches to act as vectors of medically important fungal pathogens on their external surfaces.MethodsCockroaches were captured from the main Intensive care unit (ICU), burn unit, adult surgical wards , pediatric oncology wards, intern hostel kitchen and the central kitchen of a national referral teaching hospital in Tanzania. Normal saline washings from the external surface of cockroaches were cultured on standard mycological media to facilitate isolation and identification of medically important molds and yeasts. Susceptibility of Candida sp isolates to fluconazole was tested using the CLSI M27-A3 microdilution method.ResultsAt total of 72 cockroaches were captured from various sites of the hospital between February and March 2015. All cockroaches captured were shown to carry medically importance fungi. A total of 956 medically important fungi were isolated, 57.9 % were Candida sp., 23.2%, Aspergillus sp. , 3.1% Cladosporium sp., 1.8% Rhizopus sp., 1.2 % Geotrichum sp., 0.9 % Pencillum sp. , 0.7% Alternaria sp, 0.6 % Fusarium sp , 0.3% Mucor sp and 10.1% others. Aspergillus fumigatus (50.0%) was the most commonly isolated followed by Aspergillus niger ( 15.8%) among the Aspergillus isolates.Over 16.3 % of the Candida isolates not intrinsically resistant to fluconazole showed resistance to this drug.mResistance was most frequently found in Candidapseudotropicalis (23.8%) and Candida glabarata (20.0%) and least in isolates of Candida albicans ( 6.3%). ConclusionThe external surfaces of cockroaches from this hospital may act as reservoirs of medically important opportunistic fungi exhibiting resistance to fluconazole.

scholarly journals FUNGOS ASSOCIADOS À CASCA DO CAULE DE Platanus orientalis L.

2004 ◽  
Vol 26 (54) ◽  
Author(s):  
Denise Zelak Leite Bastos ◽  
Ida Chapaval Pimentel ◽  
Christian Dykstra ◽  
Carlos Eduardo Kania ◽  
Juarez Gabardo ◽  
...  
Keyword(s):  
Sabouraud Dextrose Agar ◽  
Trichoderma Sp ◽  
Platanus Orientalis ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

Para posterior emprego na biotransformação do ácido betulínico e ácido betulônico, foram isolados fungos epifíticos e endofíticos da casca de caule de Platanus orientalis L. de acordo com metodologia específica. Para cada experimento foram utilizadas 800 amostras cortadas em pequenos pedaços e plaqueadas em dois meios de culturas diferentes: BDA (batata-dextrose-ágar) e SDA (sabouraud-dextrose-ágar). Os dados foram expressos em incidência de aparecimento do fungo. Foi realizado teste qui-quadrado (÷˝) para verificar se algum dos meios favoreceu o isolamento e cultivo dos fungos isolados. Foram identificados 13 gêneros de fungos epifíticos: Mucor sp.; Rhizopus sp.; Dematium sp.; Chaetophoma sp.; Arthobotrys sp.; Trichoderma sp.; Drecheslera sp.; Nigrospora sp.; Fusarium sp.; Pestalotia sp.; Rhizomucor sp.; Aspergillus sp. e Alternaria sp. Constatou-se que Mucor sp., Rhizopus sp. apresentaram as maiores incidências nos dois meios. Oito gêneros foram identificados para os fungos endofíticos: Mucor sp.; Stachibotrys sp.; Trichophyton sp.; Trichoderma sp.; Aspergillus sp.; Phoma sp.; Fusarium sp. e Curvularia sp. Observaram-se as maiores incidências dos fungos: Mucor sp. presente nos dois meios; Stachibotrys sp. em BDA; Trichophyton sp. em SDA. Pôde-se comprovar pelo teste (÷˝) que para alguns fungos a alteração do meiofoi significativa.

scholarly journals Actividad antifúngica de propóleos obtenidos en tres provincias de Cuba sobre hongos contaminantes en cultivo de tejidos vegetales

2014 ◽  
Vol 23 (6) ◽  
pp. 3-9
Author(s):  
René Dionisio Cupull-Santana ◽  
Remigio Cortés-Rodríguez ◽  
Ervelio Eliseo Olazábal-Manso ◽  
Carlos Alberto Hernández Medina
Keyword(s):  
Apis Mellifera ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp

La investigación se realizó con el objetivo de evaluar las características fisicoquímicas y la actividad antifúngica de propóleos de Apis mellifera, provenientes de las provincias de Villa Clara, Cienfuegos y Sancti Spíritus, Cuba. Se utilizó el método de recolección por raspado y se estableció el contenido de cera, ceniza, material insoluble y resina de los propóleos crudos. Además, se evaluó la actividad antimicrobiana in vitro frente a los hongos Aspergillus sp., Penicillium sp., Fusarium sp., Trichoderma sp., Colletotrichum sp. y Monilia sp.; los extractos presentaron alta actividad antifúngica en un amplio rango de concentraciones; además, los parámetros fisicoquímicos presentaron valores semejantes y dentro de los establecidos por regulaciones internacionales.

scholarly journals Air Quality Assessment of Port Harcourt Urban Slums and Health Implications

2021 ◽  
pp. 50-57
Author(s):  
H. O. Stanley ◽  
M. E. Amesi
Keyword(s):  
Air Quality ◽  
Fungal Species ◽  
Urban Slums ◽  
Candida Sp ◽  
Suspended Particulate ◽  
Port Harcourt ◽  
Microbiological Parameters ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

This study was conducted to assess the outdoor air quality of some urban slums in Port Harcourt. Six sampling sites were selected, from the Port Harcourt urban slums; two sites from each slum represented with a suffix 1 or 2.  The slums are designated Marine base (#1 and #2), RSU BG, Obudu 2, Bundu (#1 and #2). The air quality was analyzed using portable handheld air quality analyzer and the microbiological parameters were determined by standard cultural method. The study revealed that the sampled sites were laden with bacterial and fungal species. namely; Klebsiella sp., Micrococcus sp., Escherichia sp., Pseudomonas sp., Baccilus sp., Aeromonas sp., Streptococus sp., Serratia sp., Aerococcus sp., Proteus sp. Penicillium sp., Fusarium sp., Candida sp., Aspergillus sp., Mucor sp., Rhizopus sp. and Tricorderma sp. Highest obtained noise level was at Marine base 1 which was  66 db, highest relative humidity of 54.8% at RSU BG, CO2  (ppm) values of 4.8, 80, 796, 850, 638, 698 for Marine base 2, Marine base 1, Obudu 2, RSU BG, Bundu 1 and Bundu 2 respectively. The values for NO2 (ppm) was (0.05, 0.053, 0.071, 0.022, 0.035, 0.023), suspended particulate matter (ppm) was (7.1, 8.7, 9.5, 9.5, 6.2, 6.2), SO2 (ppm) was (0.42, 0.15, 0.50, 0.34, 1.26, 0.41) CO (ppm) was (4.8, 1.7, 2.2, 3.0, 3.9, 3.6) and volatile organic compound (ppm) was (1.0, 1.1, 0.9, 75 and 1.2). This study has shown that Port Harcourt urban slums are experiencing some degree of contamination not acceptable for healthy living that requires attention to curb. These areas require all-round improvement in sanitation.   M Give one sentence on methodology.

scholarly journals Isolamento de fungos filamentosos em água utilizada em uma unidade de hemodiálise

2007 ◽  
Vol 40 (3) ◽  
pp. 326-331 ◽  
Author(s):  
Samuel Dutra Varo ◽  
Carlos Henrique Gomes Martins ◽  
Miguel Jorge de Oliveira Cardoso ◽  
Flávio Garcia Sartori ◽  
Lílian Bueno Montanari ◽  
...  
Keyword(s):  
Sao Paulo ◽  
São Paulo ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp

A despeito da relativa freqüência de infecções fúngicas oportunísticas em pacientes sob hemodiálise, os reservatórios ambientais destes permanecem desconhecidos, embora alguns estudos recentes tenham correlacionado o suprimento de água como fonte desses microrganismos. O objetivo deste trabalho foi monitorar a qualidade micológica do sistema hídrico de uma Unidade de Hemodiálise, do interior do Estado de São Paulo, Brasil, no período entre abril e julho de 2006. Foram coletadas amostras (15), de 1000mL em 7 pontos de distribuição de água empregando-se técnica da membrana filtrante (0,45µm). Foram isolados 116 fungos filamentosos, dos quais 47 (40,5%) Trichoderma sp, 29 (25%) Cladosporium sp, 16 (13,8%) Aspergillus sp e 11 (9,5%) Fusarium sp. Mediante os resultados, sugerimos que suprimentos de água para Unidades de Hemodiálise devam ser monitorados também quanto ao aspecto micológico, adotando-se medidas profiláticas eficazes que minimizem a exposição destes pacientes imunodeficientes a fontes aquáticas ambientais contaminadas.

scholarly journals Effect of the Nematode-Trapping Fungus Dactylaria Brochopaga and the Nematode Egg Parasitic Fungus Verticilium Chlamydosporium in Controlling Citrus Nematode Infesting Mandarin, And Interrelationship with the Co Inhabitant Fungi

2018 ◽  
Vol 7 (3.32) ◽  
pp. 19
Author(s):  
NOWEER E.M.A ◽  
. .
Keyword(s):  
Soil Samples ◽  
Parasitic Fungus ◽  
Citrus Reticulata ◽  
Successive Treatment ◽  
Tylenchulus Semipenetrans ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Mixed Compound ◽  
Rhizopus Sp ◽  
Citrus Nematode

In a field experiment, the nematode-trapping fungus Dactylaria brochopaga and the nematode egg parasitic fungus Verticilium chlamydosporium , was evaluated against the citrus nematode, Tylenchulus semipenetrans infesting Mandarin, Citrus reticulata, , the mixed compound was applied in October 2016 at the rate ½ kg/tree, and root and soil samples were collected monthly until the next October, 2017. Successive treatment of the same mixed compound was added in the end of April 2017. For the comparison, Vydate 24% L. was applied as well as non treated check trees were left. Data revealed that the mixed compound treatment greatly affected the citrus nematode numbers both in soil and roots, in comparing with those of Vydate or induced by mixed compound was 97% and 70%; respectively in soil and roots. Rates of reproduction increase of the citrus nematode also reached 3% and 30% in both soil and roots; respectively. Vydate treatment resulted in a relatively lesser percentages. Growth of the concomitant fungus, Trichoderma sp. was increased specially in the last samples of October 2017, however those of fungi; Aspergillus flavus, Fusarium sp. And Rhizopus sp. was reduced, due to mixed compound treatment. Aspergillus niger and Penicillium sp. were not affected by the presence of the mixed compound. Vydate did not affect the co inhabitant fungi to a great extent. 

scholarly journals Transporte, patogenicidade e transmissibilidade de fungos associados às sementes de pinhão manso

2011 ◽  
Vol 33 (4) ◽  
pp. 663-670 ◽  
Author(s):  
Danila Alves Corrêa de Sá ◽  
Gil Rodrigues dos Santos ◽  
Gleiber Quintão Furtado ◽  
Eduardo Andréa Lemus Erasmo ◽  
Ildon Rodrigues do Nascimento
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Minas Gerais ◽  
Sao Paulo ◽  
São Paulo ◽  
Penicillium Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

Objetivou-se determinar a taxa de transporte de população de fungos associados às sementes de pinhão manso, a patogenicidade desses microrganismos a plântulas e frutos e a transmissibilidade fruto-semente e semente-plântula. Avaliaram-se a taxa de transporte, por meio de blotter test, de sementes produzidas nos estados de Minas Gerais, São Paulo, Bahia e Tocantins. As sementes foram submetidas aos tratamentos: sem desinfestação com tegumento (SDCT), sem desinfestação sem tegumento (SDST), com desinfestação com tegumento (CDCT) e com desinfestação sem tegumento (CDST). A incidência (%) dos fungos foi avaliada sob microscópio estereoscópico binocular. Para o teste de patogenicidade em plântulas e frutos inocularam-se suspensões de 10(6) esporos e discos de BDA com micélio, respectivamente. Para os fungos fitopatogênicos avaliaram-se a transmissibilidade fruto-semente e semente-plântula. O tratamento SDCT permitiu a detecção de maior número de fungos. Os fungos identificados foram Colletotrichum gloeosporioides, C. capsici, Curvularia sp., Verticillium sp., Fusarium sp., Penicillium sp., Aspergillus sp., A. niger e Rhizopus sp. Apenas as espécies de Colletotrichum são patogênicas às plântulas e frutos. Para ambas espécies há transmissibilidade fruto-semente, entretanto não é observada transmissão semente-plântula.

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