Antiplasmodial Activity of Methanolic Extract of Achyranthes aspera Shoot against Plasmodium berghei Infection in Albino Mice

This study aims at investigating the antiplasmodial activity of methanolic extract of Asparagusofficinalis L. stem on Plasmodium berghei infected mice. To investigate this, the mice were infected withP.berghei to cause malaria. The mice were simultaneously given oral doses (20, 40 and 60 mg/kg bodyweight) of methanolic extract of A. officinalis L. stem. The phytochemical constituents of the extractrevealed the presence of alkaloids, phenolics, cardiac glycosides, flavonoids, terpenoid and steroid. Theextract administered to the infected mice significantly suppressed the parasite. The extract also significantly(P<0.05) reduced the activities of serum aspatate aminotransferase (AST), alkaline phosphate (ALP),alanine aminotransferase (ALT). White blood corpuscles (WBC), red blood corpuscles (RBC), hemoglobin(HGB), packed cell volume (PCV), platelets (PLT) and the mean corpuscular hemoglobin concentration(MCHC) showed significant (P<0.05) increase after the administration of the extract while mean corpuscularvolume (MCV) and the mean corpuscular hemoglobin (MCH) showed significant (P<0.05) reduction.Present findings suggests that the plant extract contains phytochemicals that have antiplasmodial andhepatoprotective properties.

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Antiplasmodial activity of eco-friendly synthesized palladium nanoparticles using Eclipta prostrata extract against Plasmodium berghei in Swiss albino mice

Parasitology Research ◽

10.1007/s00436-015-4318-1 ◽

2015 ◽

Vol 114 (4) ◽

pp. 1397-1406 ◽

Cited By ~ 39

Author(s):

Govindasamy Rajakumar ◽

Abdul Abdul Rahuman ◽

Ill-Min Chung ◽

Arivarasan Vishnu Kirthi ◽

Sampath Marimuthu ◽

...

Keyword(s):

Plasmodium Berghei ◽

Palladium Nanoparticles ◽

Antiplasmodial Activity ◽

Swiss Albino Mice ◽

Eclipta Prostrata ◽

Albino Mice

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Antiplasmodial Activity of Ethanolic Leaf Extract of Cymbopogon citratus (DC) Stapf in Swiss Albino Mice Infected with Plasmodium berghei NK 65

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2020/v8i330193 ◽

2021 ◽

pp. 27-38

Author(s):

E. O. Dada ◽

R. O. Adebayo

Keyword(s):

Body Weight ◽

Acute Toxicity ◽

Plasmodium Berghei ◽

Leaf Extract ◽

Negative Control ◽

Antiplasmodial Activity ◽

Cymbopogon Citratus ◽

Parasitaemia Level ◽

Albino Mice ◽

Ethanolic Leaf Extract

The study assessed the antiplasmodial activity of the ethanolic leaf extract of Cymbopogon citratus on chloroquine sensitive Plasmodium berghei in mice. Standard methods were used to determine the bioactive components of the leaf extract, acute toxicity test and antiplasmodial activity. Mice obtained (of body weight 20-25 g) were housed and acclimatized for seven days at room temperature before the commencement of the experiment. A total of 16 albino mice were randomized into four groups of four mice each for acute toxicity while 35 were grouped into five groups of seven mice each for antiplasmodial activity. All the groups 1-5 were infected with P. berghei and were treated for six consecutive days with leaf extract dosage of 200, 400 and 800 mg/kg, standard antimalarial drug (chloroquine) as positive control and normal saline as negative control respectively. Phytochemical screening/ bioactive compounds of the leaf extract reveals the presence of saponins (10.3 mg/g), tannins (2.38 mg/g), flavonoids (1.87 mg/g), terpenoids (19.12 mg/g), steroids (6.21 mg/g) and glycosides (19.9 mg/g) as secondary metabolites. The leaf extract revealed decrease in body weight of the infected mice and did not show any toxicity at all dosage levels used. The antiplasmodial investigation revealed a decrease in percentage parasitaemia level in mice of extract treated groups compared with mice infected and not treated. The parasitaemia reduction was higher in 800 mg/kg than 200 mg/kg and 400 mg/kg. This significant decrease (P<0.05) in percentage parasitaemia level in the study was dose and time-dependent. The extract showed significant (p<0.05) antiplasmodial activity and could serve as possible candidates for the development of new effective drugs for the treatment of malaria.

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Phytochemical Analysis of Guiera senegalensis J.F. Gmel Extract and its Anti-Plasmodial Properties on Wister Albino Mice via Oral Route

International Journal of Pharmacology Phytochemistry and Ethnomedicine ◽

10.18052/www.scipress.com/ijppe.13.35 ◽

2019 ◽

Vol 13 ◽

pp. 35-44

Author(s):

I.H. Ifijen ◽

A.U. Mamza ◽

K.A. Fasina ◽

J.I. Omoruyi ◽

Esther U. Ikhuoria

Keyword(s):

Acute Toxicity ◽

Plasmodium Berghei ◽

Mammalian Cells ◽

Oral Route ◽

Antiplasmodial Activity ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Standard Drug ◽

Guiera Senegalensis ◽

Albino Mice

Guiera senegalensis J.F. Gmel (Combretaceae) is acclaimed as a common herbal antipyretic and anti-malarial among some tribal groups in northern Nigeria. The aim of this study was to investigate the phytochemical constituents, antiplasmodial activity and the acute toxicity of G. senegalensis on mammalian cells. Aqueous ethanolic leaf extracts (AELE) of the plant were tested for the presence of phytochemicals including alkaloids, carbohydrates, flavonoids, cardiac glycoside, glycosides, phenolic acids, saponins and tannins using standard techniques. The AELE was then screened for acute toxicity on Wister albino mice (WAM) weighing between 30-35g and its antiplasmodial activity against Plasmodium berghei (the causative agent of malaria in some mammals). Phytochemical screening revealed the presence of carbohydrate, phenols, flavonoids, abundant tannins, alkaloid, saponins with the absence of cardiac glycosides in the AELE. Also, it was observed that G. senegalensis extracts had no toxic effect on the WAM across administered doses of 100, 200 and 400 mg/kg. From the prophylactic study, it was found that the AELE dosage of 200 mg/kg was most effective in the inhibition of P. berghei when compared with the control than other dosages tested (100 and 200 mg/kg). The extract also exhibited a better anti-plasmodial property (80% inhibition) in the curative study compared to the standard drug (Chloroquine diphosphate) (52%), inhibiting at the graded doses of AELE. It can, therefore, be concluded from this study that G. senegalensis extract possesses essential phytochemicals, resulting in antiplasmodial effect against Plasmodium berghei.

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Antiplasmodial Activity of Ethanolic Extract of Neem Leaf (Azadirachta indica) in Albino Mice Infected with Plasmodium berghei

International Archives of Clinical Pharmacology ◽

10.23937/2572-3987.1510024 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Joseph Afolabi Olajide ◽

Adepeju Simon-Oke Iyabo ◽

I Oladokun Oluwatobiloba

Keyword(s):

Azadirachta Indica ◽

Plasmodium Berghei ◽

Ethanolic Extract ◽

Antiplasmodial Activity ◽

Albino Mice

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Studies on antibacterial potential and phytochemical screening of different extract of Achyranthes aspera

International Journal of Bioassays ◽

10.21746/ijbio.2016.12.005 ◽

2016 ◽

Vol 5 (12) ◽

pp. 5138

Author(s):

Shyamji Shukla* ◽

Priyanka Soni ◽

Harish K. Kewat

Keyword(s):

Methanolic Extract ◽

Salmonella Typhi ◽

Maximum Activity ◽

Phytochemical Screening ◽

Bacterial Strains ◽

Antibacterial Compounds ◽

Achyranthes Aspera ◽

Biological Sources ◽

Almost All ◽

Antibacterial Potential

There is an alarming increase in the problem of resistance towards antibiotics amongst most of the pathogenic bacterial strains in recent years. This has drawn the attention of researchers around the world to search for novel and eco-friendly antibacterial compounds. Several biological sources have been explored in this respect but medicinal plants have taken a centre stage out of all. Plants have been known as a reservoir of number of bioactive compounds specially the antibacterial ones since time immemorial. Therefore, the present investigation was undertaken to analyze the antibacterial potential of the medicinal plant Achyranthes aspera. This study revealed that highest antibacterial activity was observed in the methanolic extract of stem against almost all test Bacteria. It showed maximum activity against E.coli (30 mm), followed by S. aureus (28 mm), Enterococcus sp.(25mm), Salmonella typhi ( 20 mm) and least activity was recorded in same extract against K.pneumoniae (6 mm). Four phytochemicals were screened in various solvent extracts. They are alkaloid, flavonoids, saponins and tannins.

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