scholarly journals PCR-RFLP analysis of bacterial 16S rDNA from a typical garden soil in Taihu region

2006 ◽  
Vol 14 (4) ◽  
pp. 345 ◽  
Author(s):  
Qihui Teng
Keyword(s):  
16S Rdna ◽  
Rflp Analysis ◽  
Garden Soil ◽  
Pcr Rflp

scholarly journals 16S rDNA Genotyping of Lactic Acid Bacteria Using PCR-RFLP Analysis

2017 ◽  
Vol 64 (7) ◽  
pp. 355-364
Author(s):  
Hideyuki Tamakawa ◽  
Yoshihito Ito
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
16S Rdna ◽  
Rflp Analysis ◽  
Pcr Rflp

A rapid method for identification of typical Leuconostoc species by 16S rDNA PCR-RFLP analysis

2003 ◽  
Vol 55 (1) ◽  
pp. 295-302 ◽  
Author(s):  
Jichan Jang ◽  
Bongjoon Kim ◽  
Jongho Lee ◽  
Hongui Han
Keyword(s):  
16S Rdna ◽  
Rapid Method ◽  
Rflp Analysis ◽  
16S Rdna Pcr ◽  
Pcr Rflp

scholarly journals Genetic Heterogeneity ofAlicyclobacillusStrains Revealed by RFLP Analysis ofvdcRegion andrpoBGene

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Agnieszka Dekowska ◽  
Jolanta Niezgoda ◽  
Barbara Sokołowska
Keyword(s):  
Cluster Analysis ◽  
16S Rdna ◽  
Genetic Heterogeneity ◽  
Reference Strain ◽  
Rflp Analysis ◽  
The Other ◽  
16S Rdna Gene ◽  
Restriction Patterns ◽  
Pcr Rflp ◽  
Reference Strains

PCR-RFLP targeting of the 16S rDNA andrpoBgenes, as well as thevdcregion, was applied to identify and differentiate between the spoilage and non-spoilageAlicyclobacillusspecies. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates, and fresh apples were subject to study. Hin6I restriction patterns of the 16S rDNA gene enabled distinguishing between all the species analyzed, while therpoBgene andvdcgene cluster analysis also revealed that there were two major types among theA. acidoterrestrisisolates, one similar to the reference strainA. acidoterrestrisDSM 2498, and the other similar to the reference strainA. acidoterrestrisATCC 49025. Heterogeneity was also observed among theA. acidocaldariusisolates. RFLP analysis of the 16S rDNA andrpoBgenes, as well asvdcregion, can be used successfully in the identification and research of intraspecies heterogeneity of theAlicyclobacillusspecies.

Molecular authentication of Thai medicinal plant Khamin khruea by PCR-RFLP analysis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
P Rojsanga ◽  
W Gritsanapan ◽  
W Leelamanit ◽  
S Sukrong
Keyword(s):  
Medicinal Plant ◽  
Rflp Analysis ◽  
Molecular Authentication ◽  
Pcr Rflp

Bacteria diversity in paddy field soil by 16S rDNA-RFLP analysis in Ningxia

2008 ◽  
Vol 16 (6) ◽  
pp. 586 ◽  
Author(s):  
Zhang Jianping ◽  
Dong Naiyuan ◽  
Yu Haobin ◽  
Zhou Yongjun ◽  
Lu Yongliang ◽  
...  
Keyword(s):  
16S Rdna ◽  
Paddy Field ◽  
Rflp Analysis ◽  
Field Soil ◽  
Bacteria Diversity ◽  
Paddy Field Soil

Genetic and Biochemical Markers of Ovarian Function and Their Impact on Women Reproductive Status

2020 ◽  
Vol 71 (10) ◽  
pp. 212-217
Author(s):  
Adina-Elena Tanase ◽  
Roxana Popescu ◽  
Mircea Onofriescu ◽  
Roxana Daniela Matasariu
Keyword(s):  
Ovarian Function ◽  
Rflp Analysis ◽  
Fsh Receptor ◽  
Gene Encoding ◽  
Female Population ◽  
Fshr Gene ◽  
Pcr Rflp ◽  
Regulation Mechanisms ◽  
American Society ◽  
Dependent Mechanism

Endometriosis is a disease very common nowadays affecting 1-2% of the female population, by estrogen-dependent mechanism. The identification of mutations in the gene encoding for the FSH receptor (FSHR) has been reported since 1995. Physiology teaches us that follicle-stimulating hormone (FSH) is a hormone that is vital in the steroidogenesis regulation mechanisms, while FSH receptor (FSHR) activation helps to promote folliculogenesis and estrogensynthesis. Therefore, studies to show if there are any correlations between endometriosis and FSHR are acquired. Genotyping of FSHR gene polymorphisms were performed using PCR - Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. We analysed a total of 78 patients, 44 infertile patients with endometriosis and 34 controls (non-infertile, pregnant patients). The endometriosis group included women with diagnosis of endo-metriosis confirmed by laparoscopy and /or laparotomy and histological evidence of disease with the endometriosis staging according to American Society for Reproductive Medicine (ASRM). Corroborated with the severity of endometriosis, A919G and A2039G tests found that 71.4% of the M (GG) results were associated with primary infertility, not statistically significant (p=0.994) and 42.9% of the total M results had moderate or severe forms of endometriosis (p = 0.185). The genetic involvement in different pathologies such as endometriosis, has yet to be understood, but knowing more about its mechanism, will help physician target the disease at a more profound level.

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