Preparation of functional calcium phosphate nanoparticles for vaccine delivery

Effects of the CpG molecular structure on the mineralization and immunostimulation efficacy of calcium phosphate nanoparticles are explored for vaccine delivery applications.

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Calcium Phosphate Nanoparticles for Transcutaneous Vaccine Delivery

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2013.1545 ◽

2013 ◽

Vol 9 (1) ◽

pp. 132-141 ◽

Cited By ~ 33

Author(s):

Preety Sahdev ◽

Satheesh Podaralla ◽

Radhey S. Kaushik ◽

Omathanu Perumal

Keyword(s):

Calcium Phosphate ◽

Vaccine Delivery ◽

Calcium Phosphate Nanoparticles

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The Potential of Calcium Phosphate Nanoparticles as Adjuvants and Vaccine Delivery Vehicles

Frontiers in Materials ◽

10.3389/fmats.2021.788373 ◽

2021 ◽

Vol 8 ◽

Author(s):

Zhe Sun ◽

Wenyi Li ◽

Jason C. Lenzo ◽

James A. Holden ◽

Michael J. McCullough ◽

...

Keyword(s):

Immune Response ◽

Calcium Phosphate ◽

Vaccine Development ◽

Vaccine Delivery ◽

Vaccine Design ◽

Cost Effective ◽

Research Groups ◽

Immunological Effects ◽

Calcium Phosphate Nanoparticles ◽

Delivery Vehicles

Vaccination is one of the most efficacious and cost-effective ways to protect people from infectious diseases and potentially cancer. The shift in vaccine design from disrupted whole pathogens to subunit antigens has brought attention on to vaccine delivery materials. For the last two decades, nanotechnology-based vaccines have attracted considerable attention as delivery vehicles and adjuvants to enhance immunogenicity, exemplified with the current COVID vaccines. The nanoparticle vaccines display unique features in protecting antigens from degradation, controlled antigen release and longer persisting immune response. Due to their size, shape and surface charge, they can be outstanding adjuvants to achieve various immunological effects. With the safety and biodegradable benefit of calcium phosphate nanoparticles (CaP NPs), they are an efficient carrier for vaccine design and adjuvants. Several research groups have studied CaP NPs in the field of vaccination with great advances. Although there are several reports on the overview of CaP NPs, they are limited to the application in biomedicine, drug delivery, bone regeneration and the methodologies of CaP NPs synthesis. Hence, we summarised the basic properties of CaP NPs and the recent vaccine development of CaP NPs in this review.

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One-pot synthesis of injectable methylcellulose hydrogel containing calcium phosphate nanoparticles

Carbohydrate Polymers ◽

10.1016/j.carbpol.2016.10.055 ◽

2017 ◽

Vol 157 ◽

pp. 775-783 ◽

Cited By ~ 22

Author(s):

Hanna Park ◽

Min Hee Kim ◽

Young Il Yoon ◽

Won Ho Park

Keyword(s):

Calcium Phosphate ◽

One Pot ◽

One Pot Synthesis ◽

Calcium Phosphate Nanoparticles

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Calcium phosphate phase transition in the presence of Aminosilane

Acta Crystallographica Section A Foundations and Advances ◽

10.1107/s205327331409932x ◽

2014 ◽

Vol 70 (a1) ◽

pp. C67-C67

Author(s):

Babak Mostaghaci ◽

Brigitta Loretz ◽

Robert Haberkorn ◽

Guido Kickelbick ◽

Claus-Michael Lehr

Keyword(s):

Phase Transition ◽

Calcium Phosphate ◽

Blood Compatibility ◽

Transfection Efficiency ◽

Hek293 Cells ◽

Step Method ◽

Calcium Phosphate Nanoparticles ◽

Amine Groups ◽

The Impact

Calcium phosphate has been the point of interest for in vitro gene delivery for many years because of its biocompatibility and straight forward application. However, there are some limitations regarding in vivo administration of these particles mostly because of vast agglomeration of the particles and lack of strong bond between the particles and pDNA. We introduced a simple single step method to functionalize calcium phosphate nanoparticles with Aminosilanes having a different number of amine groups. The nanoparticles were characterized chemically and structurally and their toxicity and interaction with pDNA were studied as well. Results revealed that different crystalline phase of calcium phosphate nanoparticles (Brushite and Hydroxyapatite) with a size below 150 nm were prepared, depending on conditions of synthesis and phase, each with a narrow size distribution. The aminosilane agents caused oriented nucleation and growth of crystallites and can decrease the pH for producing hydroxyapatite phase. The phenomenon could be revealed with the presence of anisotropy in the structure of synthesized hydroxyapatite. The number of amine groups in the Aminosilane agent could change the phase transition pH. Brushite particles revealed to have stronger interaction with pDNA mostly because of their higher positive surface charge. Both particles showed blood compatibility and negligible toxicity. Transfection experiment revealed the capability of both brushite and hydroxyapatite particles to transfect A549 and HEK293 cells. The new modified nanoparticles can be stored in a dried state and re-dispersed easily at the time of administration. Moreover, the transfection efficiency is higher in comparison with conventional calcium phosphate. This study showed the impact of presence and type of the modifying agent on the crystal structure and the amount of surface functionalization of nanoparticles, which in consequence influenced their interaction with cells.

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Transfection of cells with custom-made calcium phosphate nanoparticles coated with DNA

Journal of Materials Chemistry ◽

10.1039/b401644k ◽

2004 ◽

Vol 14 (14) ◽

pp. 2213 ◽

Cited By ~ 104

Author(s):

T. Welzel ◽

I. Radtke ◽

W. Meyer-Zaika ◽

R. Heumann ◽

M. Epple

Keyword(s):

Calcium Phosphate ◽

Custom Made ◽

Calcium Phosphate Nanoparticles

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Urea-Doped Calcium Phosphate Nanoparticles as Sustainable Nitrogen Nanofertilizers for Viticulture: Implications on Yield and Quality of Pinot Gris Grapevines

Agronomy ◽

10.3390/agronomy11061026 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1026

Author(s):

Federica Gaiotti ◽

Marco Lucchetta ◽

Giacomo Rodegher ◽

Daniel Lorenzoni ◽

Edoardo Longo ◽

...

Keyword(s):

Calcium Phosphate ◽

Nitrogen Source ◽

Foliar Application ◽

N Fertilization ◽

Plant Nitrogen ◽

Yield And Quality ◽

Berry Quality ◽

Potential Benefits ◽

Calcium Phosphate Nanoparticles

In recent years, the application of nanotechnology for the development of new “smart fertilizers” is regarded as one of the most promising solutions for boosting a more sustainable and modern grapevine cultivation. Despite showing interesting potential benefits over conventional fertilization practices, the use of nanofertilizers in viticulture is still underexplored. In this work, we investigated the effectiveness of non-toxic calcium phosphate nanoparticles (Ca3(PO4)2∙nH2O) doped with urea (U-ACP) as a nitrogen source for grapevine fertilization. Plant tests were performed for two years (2019–2020) on potted adult Pinot gris cv. vines grown under semi-controlled conditions. Four fertilization treatments were compared: N1: commercial granular fertilization (45 kg N ha−1); N2: U-ACP applied in fertigation (36 kg N ha−1); N3: foliar application of U-ACP (36 kg N ha−1); C: control, receiving no N fertilization. Plant nitrogen status (SPAD), yield parameters as well as those of berry quality were analyzed. Results here presented clearly show the capability of vine plants to recognize and use the nitrogen supplied with U-ACP nanoparticles either when applied foliarly or to the soil. Moreover, all of the quali–quantitative parameters measured in vine plants fed with nanoparticles were perfectly comparable to those of plants grown in conventional condition, despite the restrained dosage of nitrogen applied with the nanoparticles. Therefore, these results provide both clear evidence of the efficacy of U-ACP nanoparticles as a nitrogen source and the basis for the development of alternative nitrogen fertilization strategies, optimizing the dosage/benefit ratio and being particularly interesting in a context of a more sustainable and modern viticulture.

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