scholarly journals An effective nanosensor for organic molecules based on water-soluble mercaptopropionic acid-capped CdTe nanocrystals with potential application in high-throughput screening and high-resolution optical microscopy

2014 ◽  
Vol 5 (7) ◽  
pp. 2420 ◽  
Author(s):  
Pick-Chung Lau ◽  
Robert A. Norwood ◽  
Masud Mansuripur ◽  
Nasser Peyghambarian
Keyword(s):  
High Resolution ◽  
Optical Microscopy ◽  
High Throughput ◽  
Potential Application ◽  
High Throughput Screening ◽  
Organic Molecules ◽  
Water Soluble ◽  
Mercaptopropionic Acid ◽  
Cdte Nanocrystals

scholarly journals A deep neural network model for packing density predictions and its application in the study of 1.5 million organic molecules

2019 ◽  
Vol 10 (36) ◽  
pp. 8374-8383 ◽  
Author(s):  
Mohammad Atif Faiz Afzal ◽  
Aditya Sonpal ◽  
Mojtaba Haghighatlari ◽  
Andrew J. Schultz ◽  
Johannes Hachmann
Keyword(s):  
Neural Network ◽  
Machine Learning ◽  
Refractive Index ◽  
High Throughput ◽  
Neural Network Model ◽  
High Throughput Screening ◽  
Deep Neural Network ◽  
Organic Molecules ◽  
High Refractive Index ◽  
Computational Pipeline

Computational pipeline for the accelerated discovery of organic materials with high refractive index via high-throughput screening and machine learning.

High-throughput screening of cellular features using high-resolution light-microscopy; Application for profiling drug effects on cell adhesion

2007 ◽  
Vol 158 (2) ◽  
pp. 233-243 ◽  
Author(s):  
Yael Paran ◽  
Micha Ilan ◽  
Yoel Kashman ◽  
Sofee Goldstein ◽  
Yuvalal Liron ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
High Resolution ◽  
Light Microscopy ◽  
High Throughput ◽  
High Throughput Screening ◽  
Drug Effects

2,2′-Thiodiethanol: A new water soluble mounting medium for high resolution optical microscopy

2006 ◽  
Vol 70 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Thorsten Staudt ◽  
Marion C. Lang ◽  
Rebecca Medda ◽  
Johann Engelhardt ◽  
Stefan W. Hell
Keyword(s):  
High Resolution ◽  
Optical Microscopy ◽  
Water Soluble

scholarly journals Development of high-throughput screening assays for profiling snake venom Phospholipase A2 activity after high-resolution chromatographic fractionation

2020 ◽  
Author(s):  
Kristina B.M. Still ◽  
Julien Slagboom ◽  
Sarah Kidwai ◽  
Chunfang Xie ◽  
Bastiaan Eisses ◽  
...  
Keyword(s):  
Fatty Acid ◽  
High Resolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Proinflammatory Mediators ◽  
Ph Indicators ◽  
Snake Venom Phospholipase ◽  
Screening Assays ◽  
Animal Venoms ◽  
Phospholipase A2 Activity

AbstractMany organisms, ranging from plants to mammals, contain phospholipase A2 enzymes (PLA2s), which catalyze the production of lysophospholipids and fatty acid proinflammatory mediators. PLA2s are also common constituents of animal venoms, including bees, scorpions and snakes, and they cause a wide variety of toxic effects including neuro-, myo-, cyto-, and cardio-toxicity, anticoagulation and edema. The aim of this study was to develop a generic method for profiling enzymatically active PLA2s in snake venoms after chromatographic separation. For this, low-volume high-throughput assays for assessment of enzymatic PLA2 activity were evaluated and optimized. Subsequently, the assays were incorporated into a nanofractionation platform that combines high-resolution fractionation of crude venoms by liquid chromatography (LC) with bioassaying in 384-well plate format, and parallel mass spectrometric (MS) detection for toxin identification. The miniaturized assays developed are based on absorbance or fluorescence detection (respectively, using cresol red or fluorescein as pH indicators) to monitor the pH drop associated with free fatty acid formation by enzymatically active PLA2s. The methodology was demonstrated for assessment of PLA2 activity profiles of venoms from the snake species Bothrops asper, Echis carinatus, Echis coloratus, Echis ocellatus, Oxyuranus scutellatus and Daboia russelii russelii.

scholarly journals Corifungin, a New Drug Lead against Naegleria, Identified from a High-Throughput Screen

2012 ◽  
Vol 56 (11) ◽  
pp. 5450-5457 ◽  
Author(s):  
Anjan Debnath ◽  
Josefino B. Tunac ◽  
Silvia Galindo-Gómez ◽  
Angélica Silva-Olivares ◽  
Mineko Shibayama ◽  
...  
Keyword(s):  
Amphotericin B ◽  
High Throughput ◽  
High Throughput Screening ◽  
Plasma Membranes ◽  
Kinase Inhibitors ◽  
Water Soluble ◽  
Drug Status ◽  
Content Type ◽  
Compound Libraries

ABSTRACTPrimary amebic meningoencephalitis (PAM) is a rapidly fatal infection caused by the free-living amebaNaegleria fowleri. The drug of choice in treating PAM is the antifungal antibiotic amphotericin B, but its use is associated with severe adverse effects. Moreover, few patients treated with amphotericin B have survived PAM. Therefore, fast-acting and efficient drugs are urgently needed for the treatment of PAM. To facilitate drug screening for this pathogen, an automated, high-throughput screening methodology was developed and validated for the closely related speciesNaegleria gruberi. Five kinase inhibitors and an NF-kappaB inhibitor were hits identified in primary screens of three compound libraries. Most importantly for a preclinical drug discovery pipeline, we identified corifungin, a water-soluble polyene macrolide with a higher activity againstNaegleriathan that of amphotericin B. Transmission electron microscopy ofN. fowleritrophozoites incubated with different concentrations of corifungin showed disruption of cytoplasmic and plasma membranes and alterations in mitochondria, followed by complete lysis of amebae.In vivoefficacy of corifungin in a mouse model of PAM was confirmed by an absence of detectable amebae in the brain and 100% survival of mice for 17 days postinfection for a single daily intraperitoneal dose of 9 mg/kg of body weight given for 10 days. The same dose of amphotericin B did not reduce ameba growth, and mouse survival was compromised. Based on these results, the U.S. FDA has approved orphan drug status for corifungin for the treatment of PAM.

Development of a multiplex high-throughput diagnostic assay for the detection of strawberry crown rot diseases using high-resolution melting analysis

2021 ◽  
Author(s):  
Nan-Yi Wang ◽  
Andre Bueno Gama ◽  
Marcus Vinicius Marin ◽  
Natalia Peres
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
High Resolution Melting ◽  
High Specificity ◽  
Macrophomina Phaseolina ◽  
Disease Diagnosis ◽  
Management Program ◽  
Crown Rot ◽  
Individual Species

Rapid and accurate disease diagnosis is a prerequisite for an effective disease management program in strawberry production. In Florida, Colletotrichum spp., Phytophthora spp, and Macrophomina phaseolina are the primary microorganisms causing strawberry crown rot. Even though the diseases can be caused by different pathogens, symptoms are indistinguishable and equally devastating. To timely inform strawberry growers of diagnostic results for effective deployment of chemical control practices, we developed a multiplex high-resolution melting (HRM) assay to rapidly and accurately detect the above-mentioned pathogens. The multiplex HRM assays using three pre-designed primer pairs showed high specificity for individual species by generating specific melting peaks without cross-reaction between primers or with other common strawberry pathogens. The amplification limit of the assay was 1 pg of Colletotrichum and Phytophthora and 100 pg of M. phaseolina DNA per 10 μl reaction. However, the presence of different melting peaks was observed in mixed DNA samples and was concentration- and target DNA-dependent. A crude DNA extraction protocol was developed to allow high-throughput screening by minimizing the inhibitory effects. Moreover, we applied the HRM assay to 522 plant samples and found high correlations between conventional pathogen isolation and HRM and between singleplex and multiplex assays. Altogether, this multiplex HRM assay is specific, cost-effective, and reliable for the timely detection of strawberry crown rot pathogens.

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