scholarly journals ATF3 downmodulates its new targets IFI6 and IFI27 to suppress the growth and migration of tongue squamous cell carcinoma cells

PLoS Genetics ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. e1009283
Author(s):  
Lin Xu ◽  
Tingjian Zu ◽  
Tao Li ◽  
Min Li ◽  
Jun Mi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Growth ◽  
Squamous Cell ◽  
Stress Responses ◽  
Squamous Cell Carcinomas ◽  
Activating Transcription Factor 3 ◽  
Cell Growth And Migration ◽  
Downstream Targets ◽  
And Migration

Activating transcription factor 3 (ATF3) is a key transcription factor involved in regulating cellular stress responses, with different expression levels and functions in different tissues. ATF3 has also been shown to play crucial roles in regulating tumor development and progression, however its potential role in oral squamous cell carcinomas has not been fully explored. In this study, we examined biopsies of tongue squamous cell carcinomas (TSCCs) and found that the nuclear expression level of ATF3 correlated negatively with the differentiation status of TSCCs, which was validated by analysis of the ATGC database. By using gain- or loss- of function analyses of ATF3 in four different TSCC cell lines, we demonstrated that ATF3 negatively regulates the growth and migration of human TSCC cells in vitro. RNA-seq analysis identified two new downstream targets of ATF3, interferon alpha inducible proteins 6 (IFI6) and 27 (IFI27), which were upregulated in ATF3-deleted cells and were downregulated in ATF3-overexpressing cells. Chromatin immunoprecipitation assays showed that ATF3 binds the promoter regions of the IFI6 and IFI27 genes. Both IFI6 and IFI27 were highly expressed in TSCC biopsies and knockdown of either IFI6 or IFI27 in TSCC cells blocked the cell growth and migration induced by the deletion of ATF3. Conversely, overexpression of either IFI6 or IFI27 counteracted the inhibition of TSCC cell growth and migration induced by the overexpression of ATF3. Finally, an in vivo study in mice confirmed those in vitro findings. Our study suggests that ATF3 plays an anti-tumor function in TSCCs through the negative regulation of its downstream targets, IFI6 and IFI27.

Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells

2020 ◽  
Vol 160 (11-12) ◽  
pp. 650-658
Author(s):  
Yichen Le ◽  
Yi He ◽  
Meirong Bai ◽  
Ying Wang ◽  
Jiaxue Wu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Growth ◽  
Cancer Progression ◽  
Normal Liver ◽  
Cell Growth And Migration ◽  
And Migration ◽  
Hcc Cells

Ajuba has been found to be mutated or aberrantly regulated in several human cancers and plays important roles in cancer progression via different signaling pathways. However, little is known about the role of Ajuba in hepatocellular carcinoma (HCC). Here, we found an upregulation of Ajuba expression in HCC tissues compared with normal liver tissues, while a poor prognosis was observed in HCC patients with high Ajuba expression. Knockout of Ajuba in HCC cells inhibited cell growth in vitro and in vivo, suppressed cell migration, and enhanced the cell apoptosis under stress. Moreover, re-expression of Ajuba in Ajuba-deficient cells could restore the phenotype of Ajuba-deficient cells. In conclusion, these results indicate that Ajuba is upregulated in HCC and promotes cell growth and migration of HCC cells, suggesting that Ajuba could possibly be a new target for HCC diagnosis and treatment.

Transcription factor EB influences invasion and migration in oral squamous cell carcinomas

Oral Diseases ◽  
2018 ◽  
Vol 24 (5) ◽  
pp. 741-748 ◽  
Author(s):  
H Sakamoto ◽  
K Yamashita ◽  
K Okamoto ◽  
T Kadowaki ◽  
E Sakai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Oral Squamous Cell Carcinomas ◽  
Invasion And Migration ◽  
Transcription Factor Eb ◽  
And Migration

scholarly journals Co-expression of MDM2 and CDK4 in transformed human mesenchymal stem cells induces dedifferentiated liposarcoma potency

2018 ◽  
Author(s):  
Yu Jin Kim ◽  
Minjung Sung ◽  
Dan Bi Yu ◽  
Mingi Kim ◽  
Ji-Young Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Growth ◽  
Adipogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Dedifferentiated Liposarcoma ◽  
Cell Growth And Migration ◽  
Well Differentiated ◽  
And Migration

AbstractAmplification and overexpression of MDM2 and CDK4 are well-known diagnostic criteria of well-differentiated liposarcoma (WDLPS)/dedifferentiated liposarcoma (DDLPS). Although it was reported that depletion of MDM2 or CDK4 decreased proliferation in DDLPS cell lines, it remains unclear whether MDM2 and CDK4 induce WDLPS/DDLPS tumorigenesis. We examined whether MDM2 and/or CDK4 produce WDLPS/DDLPS using transformed human bone marrow stem cells (BMSCs), 2H and 5H, with five oncogenic hits (overexpression of hTERT, TP53 degradation, RB inactivation, c-MYC stabilization, and overexpression of HRASv12). In vitro functional experiments revealed that co-overexpression of MDM2 and CDK4 plays key roles in tumorigenesis by increasing cell growth and migration and inhibiting adipogenic differentiation potency compared to sole expression of MDM2 or CDK4. Using mouse xenograft models, we found that co-overexpression of MDM2 and CDK4 in 5H cells with five additional oncogenic mutations can develop proliferative DDLPS in vivo. Our results suggest that co-overexpression of MDM2 and CDK4 induces DDLPS tumour potency in transformed human BMSCs by accelerating cell growth and migration and blocking adipogenetic potential incooperation with multiple genetic factors.

scholarly journals Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Yupeng Feng ◽  
Qicai Liu ◽  
Junfeng Zhu ◽  
Fukang Xie ◽  
Li Li
Keyword(s):  
Cell Growth ◽  
Adverse Effects ◽  
Reporter Gene ◽  
Iron Supplementation ◽  
Cell Growth And Migration ◽  
Ferritin Heavy Chain ◽  
Mr Relaxation ◽  
And Migration

Background. An emerging MRI reporter, ferritin heavy chain (FTH1), is recently applied to enhance the contrast and increase the sensitivity of MRI in the monitoring of solid tumors. However, FTH1-overexpression-related cytotoxicity is required to be explored.Methods. By using the Tet-Off system, FTH1 overexpression was semi-quantitativiely and dynamicly regulated by doxycycline in a NPC cell line. Effects of FTH1 overexpression on the proliferation, cytotoxicity, apoptosis and migration of NPC cells were investigatedin vitro, and MR relaxation rate was measuredin vitroandin vivo.Results.In vitroandin vivooverexpression of FTH1 significantly increased the transverse relaxivity (R2), which could be enhanced by iron supplementation.In vitro, overexpression of FTH1 reduced cell growth and migration, which were not reduced by iron supplementation. Furthermore, cells were subcutaneously inoculated into the nude mice. Results showed FTH1 overexpression decreased tumor growth in the absence of iron supplementation but not in the presence of iron supplementation.Conclusion. To maximizeR2and minimize the potential adverse effects, supplementation of iron at appropriate dose is recommended during the application of FTH1 as a reporter gene in the monitoring of NPC by MRI.

CMTM3 inhibits cell growth and migration and predicts favorable survival in oral squamous cell carcinoma

Tumor Biology ◽  
2015 ◽  
Vol 36 (10) ◽  
pp. 7849-7858 ◽  
Author(s):  
Heyu Zhang ◽  
Jianyun Zhang ◽  
Xu Nan ◽  
Xuefen Li ◽  
Jiafei Qu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Growth ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cell Growth And Migration ◽  
And Migration

scholarly journals Melt Electrowritten In Vitro Radial Device to Study Cell Growth and Migration

2020 ◽  
Vol 4 (10) ◽  
pp. 2000077 ◽  
Author(s):  
Ezgi Bakirci ◽  
Natascha Schaefer ◽  
Ouafa Dahri ◽  
Andrei Hrynevich ◽  
Pamela Strissel ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Growth And Migration ◽  
And Migration
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