scholarly journals Reappraisal of Leishmanin Skin Test (LST) in the management of American Cutaneous Leishmaniasis: A retrospective analysis from a reference center in Argentina

2017 ◽  
Vol 11 (10) ◽  
pp. e0005980 ◽  
Author(s):  
Alejandro Javier Krolewiecki ◽  
Maria Cristina Almazan ◽  
Marcelo Quipildor ◽  
Marisa Juarez ◽  
Jose Fernando Gil ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Retrospective Analysis ◽  
Skin Test ◽  
Reference Center ◽  
Leishmanin Skin Test ◽  
American Cutaneous Leishmaniasis

Studies on Mediterranean leishmaniasis IV. The leishmanin skin test in cutaneous leishmaniasis

1976 ◽  
Vol 70 (1) ◽  
pp. 62-65 ◽  
Author(s):  
S. Pampiglione ◽  
P.E.C. Manson-Bahr ◽  
M. La Placa ◽  
M.A. Borgatti ◽  
F. Micheloni
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Leishmanin Skin Test

scholarly journals Histological observations on Montenegro's reaction in man

1989 ◽  
Vol 31 (4) ◽  
pp. 256-261 ◽  
Author(s):  
Wilson Mayrink ◽  
Antonio Pedro M. Schettini ◽  
Paul Williams ◽  
Pedro Raso ◽  
Paulo Araujo Magalhães ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Diagnostic Method ◽  
Previous History ◽  
Histological Changes ◽  
Histological Studies ◽  
Vaccine Trials ◽  
American Cutaneous Leishmaniasis ◽  
History Of

The Montenegro skin test is widely used as a diagnostic method for American cutaneous leishmaniasis (ACL) but little is known about the histological changes that occur in the skin after administration of the antigen. This report is based on histological studies of biopsied material obtained, from inoculation sites, 48 hours after individuals had been given intradermal injections with a standardized Montenegro antigen. The material examined was obtained from four distinctly different test groups: naturally infected patients with parasitologically proved ACL and with positive Montenegro's reaction; individuals without previous history of ACL and not previously tested with Montenegro antigen; participants in anti-ACL vaccine trials who developed positive reactions to Montenegro antigen after vaccination; other participants in vaccine trials who had negative Montenegro responses after vaccination or had served as controls in the trials. The histological pictures of each group are described and discussed. Histologically, the reactions of vaccinated individuals were indistinguishable from those with naturally acquired infections.

scholarly journals EPIDEMIOLOGICAL SURVEY ON CANINE POPULATION WITH THE USE OF IMMUNOLEISH SKIN TEST IN ENDEMIC AREAS OF HUMAN AMERICAN CUTANEOUS LEISHMANIASIS IN THE STATE OF RIO DE JANEIRO, BRAZIL

1998 ◽  
Vol 40 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Elizabeth Gloria O. Barbosa Santos ◽  
Mauro Célio A. Marzochi ◽  
Nilton Francisco Conceição ◽  
Célia Maria M. Brito ◽  
Raquel S. Pacheco
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Rio De Janeiro ◽  
Epidemiological Survey ◽  
The State ◽  
Clinical Aspects ◽  
Mucocutaneous Leishmaniasis ◽  
American Cutaneous Leishmaniasis ◽  
Epidemiological Surveys ◽  
Positive Rate

A survey for canine tegumentary leishmaniasis (CTL) has been carried out between 1986 and 1993 in seven endemic localities for American cutaneous leishmaniasis in the State of Rio de Janeiro. 270 dogs have been examined for their clinical aspects, the development of delayed hypersensitivity (DHS) with Immunoleish antigen and with immunofluorescent antibody research of IgG (IF). 28.2% of them had ulcer lesions and 3.3% had scars. The lesions consisted of single (39.5%) and mucocutaneous lesions (31.6%), multiple cutaneous (25.0%) and mucocutaneous lesions associated with cutaneous ulcers (4.0%). Twelve (15.8%) isolates from biopsies were analyzed by zimodeme and schizodeme and identified as L. (V.) braziliensis. The overall prevalence of canine infection that was evaluated with the skin test was of 40.5% and with IF it was of 25.5%. Both tests showed a high positive rate with relation to the animals with mucosal lesions, as in the case of human mucocutaneous leishmaniasis. The comparison of the two tests showed the skin test to have a better performance although there was no statistical difference (p>0.05) between them. The proportional sensitivity and specificity was of 84.0% and 74.0%, respectively. The Immunoleish skin test and IF are useful tools to be employed in CTL field epidemiological surveys.

scholarly journals Prevalence of Cutaneous Leishmaniasis in Western Highlands in Yemen

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Mohammed Musid Alkulaibi ◽  
Ahmed Mohamed Suleiman ◽  
Eltahir Awad Gasim Khalil ◽  
Maged Ahmed Al-Garadi
Keyword(s):  
Physical Examination ◽  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Cross Sectional Survey ◽  
Active Lesion ◽  
Cross Sectional ◽  
Leishmanin Skin Test

Leishmaniasis in Yemen is still not fully investigated nor well studied. Recently, outbreaks of cutaneous leishmaniasis (CL) in western highland were declared. However, there are no reports concerning the disease and the circulating species in the region. The aim of this study was to determine the prevalence of cutaneous leishmaniasis in Utmah district located in Western Highlands in Yemen. A cross-sectional survey was carried out at those highlands. For the survey, 1165 participants were subjected to Leishmanin Skin Test (LST) accompanied with direct interviews and physical examination. The overall prevalence of cutaneous leishmaniasis in the district was 18.5% and the cutaneous leishmaniasis (CL) was more frequent in the escarpments with a prevalence of 37%, including 5.5% for active lesion and 31.5% for scar of healed lesions. Children under the age of 16 years old comprised most of the CL cases (76.3%). The escarpments of western highlands in Yemen were hyperendemic areas for CL and the infection was more prevalent in children.

Surveillance for Leishmania asymptomatic infection in endemic foci of cutaneous leishmaniasis in Venezuela: a combination of leishmanin skin test and PCR using blood clots improves detection and enables identification of species

2020 ◽  
Vol 114 (6) ◽  
pp. 433-439
Author(s):  
Annhymariet Torrellas ◽  
Elizabeth Ferrer ◽  
Israel Cruz ◽  
Hector De Lima ◽  
Rafael Borges ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Internal Transcribed Spacer ◽  
Leishmania Species ◽  
Asymptomatic Infection ◽  
Negative Group ◽  
Serum Samples ◽  
Leishmanin Skin Test ◽  
Blood Clots ◽  
Identification Of Species

Abstract Background Little is known about the prevalence of asymptomatic leishmaniasis in Venezuela. The objective of this study was to quantify Leishmania asymptomatic infection in six endemic foci of cutaneous leishmaniasis (CL) in Portuguesa State, Venezuela, where no previous data were available. Methods Study of the prevalence of Leishmania asymptomatic infection was carried out in 841 individuals from six endemic foci of CL in the municipalities Sucre and Ospino, Portuguesa State. We applied the leishmanin skin test (LST) and the internal transcribed spacer 1 (ITS1) PCR to DNA from sera and blood clots of all LST-positive and 20% of LST-negative patients. Results Of 841 inhabitants tested by LST, 197 returned a positive reaction (23.42%); all of the LST-positives (197) and 121 negatives were screened by nested PCR using serum and blood clots. Among the LST-positive group, 2.54% were PCR-positive with sera, while 44.67% were positive with blood clots. In the LST-negative group, PCR was positive in 2.48% of serum samples and in 38.84% of blood clots. Conclusions It is recommended that LST and PCR on blood clots are used together to detect exposure and asymptomatic infection and for identification of the Leishmania species.

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