Acceptability of Aedes aegypti blood feeding on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru

Dengue fever (DF) is the most prevalent arthropod-borne viral disease which affects humans. DF is caused by the four dengue virus (DENV) serotypes, which are transmitted to the host by the mosquitoAedes aegyptithat has key roles in DENV infection, replication, and viral transmission (vector competence). Mosquito saliva also plays an important role during DENV transmission. In this study, we detected the presence of sialic acid (Sia) inAedes aegyptitissues, which may have an important role during DENV-vector competence. We also identified genome sequences encoding enzymes involved in Sia pathways. The cDNA forAedes aegyptiCMP-Sia synthase (CSAS) was amplified, cloned, and functionally evaluated via the complementation of LEC29.Lec32 CSAS-deficient CHO cells.AedesCSAS-transfected LEC29.Lec32 cells were able to express Sia moieties on the cell surface. Sequences related toα-2,6-sialyltransferase were detected in theAedes aegyptigenome. Likewise, we identified Sia-α-2,6-DENV interactions in different mosquito tissues. In addition, we evaluated the possible role of sialylated molecules in a salivary gland extract during DENV internalization in mammalian cells. The knowledge of early DENV-host interactions could facilitate a better understanding of viral tropism and pathogenesis to allow the development of new strategies for controlling DENV transmission.

Download Full-text

Reduction of Aedes aegypti Vector Competence for Dengue Virus under Large Temperature Fluctuations

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.12-0488 ◽

2013 ◽

Vol 88 (4) ◽

pp. 689-697 ◽

Cited By ~ 63

Author(s):

Lauren B. Carrington ◽

Stephanie N. Seifert ◽

Thomas W. Scott ◽

Louis Lambrechts ◽

M. Veronica Armijos

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Vector Competence ◽

Temperature Fluctuations ◽

Large Temperature

Download Full-text

Effect of the Topical Repellent para-Menthane-3,8-diol on Blood Feeding Behavior and Fecundity of the Dengue Virus Vector Aedes aegypti

Insects ◽

10.3390/insects9020060 ◽

2018 ◽

Vol 9 (2) ◽

pp. 60 ◽

Cited By ~ 2

Author(s):

Jugyeong Lee ◽

Diane Choi ◽

Fang Liu ◽

John Grieco ◽

Nicole Achee

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Feeding Behavior ◽

Blood Feeding ◽

Virus Vector

Download Full-text

Vector competence of Aedes aegypti and Culex quinquefasciatus from the metropolitan area of Guadalajara, Jalisco, Mexico for Zika virus

Scientific Reports ◽

10.1038/s41598-019-53117-1 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Darwin Elizondo-Quiroga ◽

Miriam Ramírez-Medina ◽

Abel Gutiérrez-Ortega ◽

Armando Elizondo-Quiroga ◽

José Esteban Muñoz-Medina ◽

...

Keyword(s):

Aedes Aegypti ◽

Culex Quinquefasciatus ◽

Zika Virus ◽

Vector Competence ◽

Virus Transmission ◽

Blood Feeding ◽

Surveillance Program ◽

Virus Spread ◽

Zikv Infection ◽

Almost All

AbstractZika virus (ZIKV) is a mosquito-borne pathogen discovered in the late 40’s in Uganda during a surveillance program for yellow fever. By 2014 the virus reached Eastern Island in the Americas, and two years later, the virus spread to almost all countries and territories of the Americas. The mosquito Aedes aegypti has been identified as the main vector of the disease, and several researchers have also studied the vector competence of Culex quinquefasciatus in virus transmission. The aim of the present study was to evaluate the vector competence of Ae. aegypti and Cx. quinquefasciatus in order to understand their roles in the transmission of ZIKV in Guadalajara, Jalisco, Mexico. In blood feeding laboratry experiments, we found that Ae. aegypti mosquitoes showed to be a competent vector able to transmit ZIKV in this area. On the other hand, we found that F0 Cx. quinquefasciatus mosquitoes are refractory to ZIKV infection, dissemination and transmission.

Download Full-text

Larval Exposure to the Bacterial Insecticide Bti Enhances Dengue Virus Susceptibility of Adult Aedes aegypti Mosquitoes

Insects ◽

10.3390/insects9040193 ◽

2018 ◽

Vol 9 (4) ◽

pp. 193 ◽

Cited By ~ 6

Author(s):

Isabelle Moltini-Conclois ◽

Renaud Stalinski ◽

Guillaume Tetreau ◽

Laurence Després ◽

Louis Lambrechts

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Adult Stage ◽

Vector Competence ◽

Life Stage ◽

Genetic Resistance ◽

Bacterial Pathogen ◽

Human Pathogens ◽

Resistant Strains ◽

Pathogen Exposure

Understanding the interactions between pathogens sharing the same host can be complicated for holometabolous animals when larval and adult stages are exposed to distinct pathogens. In medically important insect vectors, the effect of pathogen exposure at the larval stage may influence susceptibility to human pathogens at the adult stage. We addressed this hypothesis in the mosquito Aedes aegypti, a major vector of arthropod-borne viruses (arboviruses), such as the dengue virus (DENV) and the chikungunya virus (CHIKV). We experimentally assessed the consequences of sub-lethal exposure to the bacterial pathogen Bacillus thuringiensis subsp. israelensis (Bti), during larval development, on arbovirus susceptibility at the adult stage in three Ae. aegypti strains that differ in their genetic resistance to Bti. We found that larval exposure to Bti significantly increased DENV susceptibility, but not CHIKV susceptibility, in the Bti-resistant strains. However, there was no major difference in the baseline arbovirus susceptibility between the Bti-resistant strains and their Bti-susceptible parental strain. Although the generality of our results remains to be tested with additional arbovirus strains, this study supports the idea that the outcome of an infection by a pathogen depends on other pathogens sharing the same host even when they do not affect the same life stage of the host. Our findings may also have implications for Bti as a mosquito biocontrol agent, indicating that the sub-optimal Bti efficacy may have counter-productive effects by increasing vector competence, at least for some combinations of arbovirus and mosquito strains.

Download Full-text