Real-Time Dynamic Imaging of Virus Distribution In Vivo

Minimally invasive surgery is widely used because of its tremendous benefits to the patient. However, there are some challenges that surgeons face in this type of surgery, the most important of which is the narrow field of view. Therefore, we propose an approach to expand the field of view for minimally invasive surgery to enhance surgeons’ experience. It combines multiple views in real-time to produce a dynamic expanded view. The proposed approach extends the monocular Oriented features from an accelerated segment test and Rotated Binary robust independent elementary features—Simultaneous Localization And Mapping (ORB-SLAM) to work with a multi-camera setup. The ORB-SLAM’s three parallel threads, namely tracking, mapping and loop closing, are performed for each camera and new threads are added to calculate the relative cameras’ pose and to construct the expanded view. A new algorithm for estimating the optimal inter-camera correspondence matrix from a set of corresponding 3D map points is presented. This optimal transformation is then used to produce the final view. The proposed approach was evaluated using both human models and in vivo data. The evaluation results of the proposed correspondence matrix estimation algorithm prove its ability to reduce the error and to produce an accurate transformation. The results also show that when other approaches fail, the proposed approach can produce an expanded view. In this work, a real-time dynamic field-of-view expansion approach that can work in all situations regardless of images’ overlap is proposed. It outperforms the previous approaches and can also work at 21 fps.

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Real Time Dynamic Imaging and Current Targeted Therapies in the War on Cancer: A New Paradigm

Theranostics ◽

10.7150/thno.5658 ◽

2013 ◽

Vol 3 (6) ◽

pp. 437-447 ◽

Cited By ~ 13

Author(s):

Ramasamy Paulmurugan ◽

Bryan Oronsky ◽

Chad F Brouse ◽

Tony Reid ◽

Susan Knox ◽

...

Keyword(s):

Real Time ◽

Targeted Therapies ◽

Dynamic Imaging ◽

New Paradigm ◽

Time Dynamic

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Expandable and implantable bioelectronic complex for analyzing and regulating real-time activity of the urinary bladder

Science Advances ◽

10.1126/sciadv.abc9675 ◽

2020 ◽

Vol 6 (46) ◽

pp. eabc9675

Author(s):

Tae-Min Jang ◽

Joong Hoon Lee ◽

Honglei Zhou ◽

Jaesun Joo ◽

Bong Hee Lim ◽

...

Keyword(s):

Urinary Bladder ◽

Real Time ◽

Detrusor Underactivity ◽

Seamless Integration ◽

Time Activity ◽

Time Dynamic ◽

In Vivo Experiments ◽

Urinary Infections ◽

Life Threatening

Underactive bladder or detrusor underactivity (DUA), that is, not being able to micturate, has received less attention with little research and remains unknown or limited on pathological causes and treatments as opposed to overactive bladder, although the syndrome may pose a risk of urinary infections or life-threatening kidney damage. Here, we present an integrated expandable electronic and optoelectronic complex that behaves as a single body with the elastic, time-dynamic urinary bladder with substantial volume changes up to ~300%. The system configuration of the electronics validated by the theoretical model allows conformal, seamless integration onto the urinary bladder without a glue or suture, enabling precise monitoring with various electrical components for real-time status and efficient optogenetic manipulation for urination at the desired time. In vivo experiments using diabetic DUA models demonstrate the possibility for practical uses of high-fidelity electronics in clinical trials associated with the bladder and other elastic organs.

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Real-Time In Vivo Detection and Monitoring of Bacterial Infection Based on NIR-II Imaging

Frontiers in Chemistry ◽

10.3389/fchem.2021.689017 ◽

2021 ◽

Vol 9 ◽

Author(s):

Sijia Feng ◽

Huizhu Li ◽

Chang Liu ◽

Mo Chen ◽

Huaixuan Sheng ◽

...

Keyword(s):

Bacterial Infection ◽

Real Time ◽

Bacterial Infections ◽

Near Infrared ◽

Imaging System ◽

Bacterial Load ◽

Dynamic Imaging ◽

In Vivo Detection

Treatment according to the dynamic changes of bacterial load in vivo is critical for preventing progression of bacterial infections. Here, we present a lead sulfide quantum dots (PbS QDs) based second near-infrared (NIR-II) fluorescence imaging strategy for bacteria detection and real-time in vivo monitoring. Four strains of bacteria were labeled with synthesized PbS QDs which showed high bacteria labeling efficiency in vitro. Then bacteria at different concentrations were injected subcutaneously on the back of male nude mice for in vivo imaging. A series of NIR-II images taken at a predetermined time manner demonstrated changing patterns of photoluminescence (PL) intensity of infected sites, dynamically imaging a changing bacterial load in real-time. A detection limit around 102–104 CFU/ml was also achieved in vivo. Furthermore, analysis of pathology of infected sites were performed, which showed high biocompatibility of PbS QDs. Therefore, under the guidance of our developed NIR-II imaging system, real-time detection and spatiotemporal monitoring of bacterial infection in vivo can be achieved, thus facilitating anti-infection treatment under the guidance of the dynamic imaging of bacterial load in future.

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A non-invasive nanoparticles for multimodal imaging of ischemic myocardium in rats

Journal of Nanobiotechnology ◽

10.1186/s12951-021-00822-7 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Xiajing Chen ◽

Yanan Zhang ◽

Hui Zhang ◽

Liang Zhang ◽

Lingjuan Liu ◽

...

Keyword(s):

Multimodal Imaging ◽

Early Stage ◽

Heart Diseases ◽

Dynamic Imaging ◽

Ischemic Myocardium ◽

Time Dynamic ◽

Non Invasive ◽

Rat Hearts ◽

Imaging Results

Abstract Background Ischemic heart disease (IHD) is the leading cause of morbidity and mortality worldwide, and imposes a serious economic load. Thus, it is crucial to perform a timely and accurate diagnosis and monitoring in the early stage of myocardial ischemia. Currently, nanoparticles (NPs) have emerged as promising tools for multimodal imaging, because of their advantages of non-invasion, high-safety, and real-time dynamic imaging, providing valuable information for the diagnosis of heart diseases. Results In this study, we prepared a targeted nanoprobe (termed IMTP-Fe3O4-PFH NPs) with enhanced ultrasound (US), photoacoustic (PA), and magnetic resonance (MR) performance for direct and non-invasive visual imaging of ischemic myocardium in a rat model. This successfully designed nanoprobe had excellent properties such as nanoscale size, good stability, phase transformation by acoustic droplet vaporization (ADV), and favorable safety profile. Besides, it realized obvious targeting performance toward hypoxia-injured cells as well as model rat hearts. After injection of NPs through the tail vein of model rats, in vivo imaging results showed a significantly enhanced US/PA/MR signal, well indicating the remarkable feasibility of nanoprobe to distinguish the ischemic myocardium. Conclusions IMTP-Fe3O4-PFH NPs may be a promising nanoplatform for early detection of ischemic myocardium and targeted treatment under visualization for the future.

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A real-time dynamic imaging system for centrifugal microflow platforms

Measurement Science and Technology ◽

10.1088/0957-0233/19/7/075501 ◽

2008 ◽

Vol 19 (7) ◽

pp. 075501 ◽

Cited By ~ 6

Author(s):

Hsing-Cheng Chang ◽

Chingfu Tsou ◽

Chi-Chih Lai ◽

Guo-Hong Wun

Keyword(s):

Real Time ◽

Imaging System ◽

Dynamic Imaging ◽

Time Dynamic

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Real-time Dynamic Imaging Method for Flexible Boundary Sensor in Wearable Electrical Impedance Tomography

IEEE Sensors Journal ◽

10.1109/jsen.2020.2987534 ◽

2020 ◽

pp. 1-1

Author(s):

P.N Darma ◽

M.R. Baidillah ◽

M.W. Sifuna ◽

M. Takei

Keyword(s):

Real Time ◽

Electrical Impedance Tomography ◽

Electrical Impedance ◽

Dynamic Imaging ◽

Imaging Method ◽

Impedance Tomography ◽

Time Dynamic

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Comparison of Four Ultrasonic Techniques for In Vivo Estimation of Sheep Carcass Composition

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600023576 ◽

1993 ◽

Vol 1993 ◽

pp. 28-28 ◽

Cited By ~ 1

Author(s):

J.P Chadwick ◽

C.M. Yates ◽

M.G. Owen

Keyword(s):

Real Time ◽

Live Weight ◽

Dynamic Imaging ◽

Carcass Composition ◽

Equal Size ◽

List Type ◽

Velocity Of Sound ◽

Ultrasonic Techniques

1. There are many ultrasonic machines using a range of techniques varying in sophistication to measure fat and muscle depths for predicting carcass composition. An evaluation of four techniques was conducted by the Meat and Livestock Commission (MLC) to enable potential users to select the most appropriate technique based on accuracy, practicality and cost.2. A total of 150 commercial sheep comprising approximately equal numbers of castrates and ewes were evaluated in five batches of about equal size in lairage at one of two abattoirs. Live weight was recorded together with fat and muscle measurements from the Aloka, Dynamic Imaging, Dumatic and Velocity of Sound (VOS) ultrasonic techniques.3. The Aloka is a B-Mode real-time medical scanner which is currently used on cattle in Australia. The Dynamic Imaging is also a B-Mode real-time medical scanner which is currently used in MLC's Sheepbreeder Service.

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