scholarly journals Large-Scale Collection and Analysis of Full-Length cDNAs from Brachypodium distachyon and Integration with Pooideae Sequence Resources

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e75265 ◽  
Author(s):  
Keiichi Mochida ◽  
Yukiko Uehara-Yamaguchi ◽  
Fuminori Takahashi ◽  
Takuhiro Yoshida ◽  
Tetsuya Sakurai ◽  
...  
Keyword(s):  
Large Scale ◽  
Brachypodium Distachyon ◽  
Full Length

scholarly journals FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yanping Long ◽  
Zhijian Liu ◽  
Jinbu Jia ◽  
Weipeng Mo ◽  
Liang Fang ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Walls ◽  
Large Scale ◽  
Full Length ◽  
Cell Level ◽  
Root Cells ◽  
Rna Profiling ◽  
Different Types ◽  
Long Read ◽  
Single Nucleus

AbstractThe broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at a single-nucleus level in plants using isolated nuclei. Combined with 10x Genomics and Nanopore long-read sequencing, we validate the robustness of this approach in Arabidopsis root cells and the developing endosperm. Sequencing results demonstrate that it allows for uncovering alternative splicing and polyadenylation-related RNA isoform information at the single-cell level, which facilitates characterizing cell identities.

scholarly journals Large-scale collection of full-length cDNA and transcriptome analysis inHevea brasiliensis

DNA Research ◽  
2017 ◽  
pp. dsw056 ◽  
Author(s):  
Yuko Makita ◽  
Kiaw Kiaw Ng ◽  
G. Veera Singham ◽  
Mika Kawashima ◽  
Hideki Hirakawa ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Large Scale ◽  
Full Length ◽  
Full Length Cdna

scholarly journals A very straight and collimated outflow in the core of OMC-1

1991 ◽  
Vol 147 ◽  
pp. 491-493
Author(s):  
J. Schmid-Burgk ◽  
R. Güsten ◽  
R. Mauersberger ◽  
A. Schulz ◽  
T. L. Wilson
Keyword(s):  
Large Scale ◽  
Position Angle ◽  
Full Length ◽  
Hii Region ◽  
The Core ◽  
Straight Line ◽  
Outflow System

We have recently discovered a large-scale (200″) outflow system in the core of OMC-1 (fig. 1), centered about 100″ South of IRc2 and extending over some 120″ (red lobe) resp. 60″ (blue) along a position angle of —31° (Schmid-Burgk et al. 1990). The blue lobe which might actually protrude into the HII region M42 is poorly defined in CO 2-1, but the red lobe reveals a number of remarkable properties which we summarize here:The outflow is very straight and smooth. Over the full length of 120″, the center of any cross scan deviates by not more than about 1″ from a straight line. This line passes to within 2″ the peak of the submm source FIR4 of OMC-1 (Mezger, Wink and Zylka 1990) and the mm continuum peak CS3 (Mundy et al. 1986); it also cuts across the red and blue SiO-outflow lobes recently discovered some 5-10″ to either side of FIR4 (Ziurys, Wilson and Mauersberger 1990). It thus seems that the “base” of our large-scale CO jet can be seen as well.

scholarly journals Large-scale RT-PCR recovery of full-length cDNA clones

BioTechniques ◽  
2004 ◽  
Vol 36 (4) ◽  
pp. 690-700 ◽  
Author(s):  
Jia Qian Wu ◽  
Angela M. Garcia ◽  
Steven Hulyk ◽  
Anna Sneed ◽  
Carla Kowis ◽  
...  
Keyword(s):  
Large Scale ◽  
Full Length ◽  
Cdna Clones ◽  
Rt Pcr ◽  
Full Length Cdna

Marga Richter

2012 ◽  
Author(s):  
Sharon Mirchandani
Keyword(s):  
Twentieth Century ◽  
Large Scale ◽  
Chamber Ensemble ◽  
Modern Dance ◽  
Private Life ◽  
Full Length ◽  
Musical Style ◽  
Secondary Sources ◽  
Musical Works ◽  
American Composer

This is the first full-length introduction to the life and works of American composer Marga Richter (born 1926), who has written more than a hundred works for orchestra, chamber ensemble, dance, opera, voice, chorus, piano, organ, and harpsichord. Still actively composing in her eighties, Richter is particularly known for her large-scale pieces performed by ensembles such as the London Philharmonic Orchestra and the Civic Orchestra of Chicago and for other pieces performed by prominent artists including pianist Menahem Pressler, conductor Izler Solomon, and violinist Daniel Heifetz. Interspersing consideration of Richter's musical works with discussion of her life, her musical style, and the origins and performances of her works, the book documents a successful composer's professional and private life throughout the twentieth century. The book covers Richter's formative years, her influences, and the phases of her career from the 1950s to the present. Drawing extensively on interviews with Richter herself, the book also provides detailed descriptions of Richter's scores and uses reviews and other secondary sources to provide contexts for her compositions, including their relationship to modern dance, to other musical styles, and to 1970s feminism.

scholarly journals A large-scale full-length cDNA analysis to explore the budding yeast transcriptome

2006 ◽  
Vol 103 (47) ◽  
pp. 17846-17851 ◽  
Author(s):  
F. Miura ◽  
N. Kawaguchi ◽  
J. Sese ◽  
A. Toyoda ◽  
M. Hattori ◽  
...  
Keyword(s):  
Large Scale ◽  
Budding Yeast ◽  
Full Length ◽  
Full Length Cdna ◽  
Cdna Analysis

scholarly journals The Full-Length Protein Encoded by Human Cytomegalovirus Gene UL117 Is Required for the Proper Maturation of Viral Replication Compartments

2008 ◽  
Vol 82 (7) ◽  
pp. 3452-3465 ◽  
Author(s):  
Zhikang Qian ◽  
Baoqin Xuan ◽  
Te Tee Hong ◽  
Dong Yu
Keyword(s):  
Human Cytomegalovirus ◽  
Large Scale ◽  
Full Length ◽  
Wild Type ◽  
Virus Growth ◽  
Reading Frame ◽  
Early Proteins ◽  
Immediate Early Proteins ◽  
Novel Transcripts ◽  
Related Proteins

ABSTRACT Previously, two large-scale mutagenic analyses showed that mutations in the human cytomegalovirus (HCMV) gene UL117 resulted in a defect in virus growth in fibroblasts. Early transcriptional analyses have revealed several mRNAs from the UL119-UL115 region; however, specific transcripts encoding UL117-related proteins have not been identified. In this study, we identified two novel transcripts arising from the UL117 gene locus, and we reported that the UL117 open reading frame encoded the full-length protein pUL117 (45 kDa) and the shorter isoform pUL117.5 (35 kDa) as the result of translation initiation at alternative in-frame ATGs. Both proteins were expressed with early kinetics, but pUL117 accumulated at a lower abundance relative to that of pUL117.5. During HCMV infection, both proteins localized predominantly to the nucleus, and the major fraction of pUL117 localized in viral nuclear replication compartments. We constructed mutant HCMV viruses in which the entire UL117 coding sequence was deleted or the expression of pUL117 was specifically abrogated. The growth of mutant viruses was significantly attenuated, indicating that pUL117 was required for efficient virus infection in fibroblasts. Cells infected with the pUL117-deficient mutant virus accumulated representative viral immediate-early proteins and early proteins normally. In the absence of pUL117, the accumulation of replicating viral DNA was reduced by no more than twofold at early times and was indistinguishable from that of the wild type at 72 h postinfection. Strikingly, there was a 12- to 24-h delay in the development of nuclear replication compartments and a marked delay in the expression of late viral proteins. We conclude that pUL117 acts to promote the development of nuclear replication compartments to facilitate viral growth.

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