scholarly journals Na+/K+-ATPase α-subunit (nkaα) Isoforms and Their mRNA Expression Levels, Overall Nkaα Protein Abundance, and Kinetic Properties of Nka in the Skeletal Muscle and Three Electric Organs of the Electric Eel, Electrophorus electricus

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0118352 ◽  
Author(s):  
Biyun Ching ◽  
Jia M. Woo ◽  
Kum C. Hiong ◽  
Mel V. Boo ◽  
Celine Y. L. Choo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Kinetic Properties ◽  
Protein Abundance ◽  
Α Subunit ◽  
Expression Levels ◽  
Electrophorus Electricus ◽  
Electric Organs ◽  
Electric Eel ◽  
Mrna Expression Levels

scholarly journals Effect of Short-Term Endurance Exercise on COX IV and PGC-1a mRNA Expression Levels in Rat Skeletal Muscle

2019 ◽  
Vol 12 (3) ◽  
pp. 1309-1316 ◽  
Author(s):  
Nova Sylviana ◽  
Christina Natalia ◽  
Hanna Goenawan ◽  
Yuni Susanti Pratiwi ◽  
Iwan Setiawan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Endurance Exercise ◽  
Mitochondrial Biogenesis ◽  
Control Group ◽  
Muscle Adaptation ◽  
Rat Skeletal Muscle ◽  
Short Term ◽  
Expression Levels ◽  
Mrna Expression Levels

Endurance exercise induces specific skeletal muscle adaptation by increasing mitochondrial oxidative phosphorylation eficiency and mitochondrial biogenesis. Many previous studies suggesting both PGC-1a and COX IV as a potential biomarker of skeletal muscle adaptation induced by exercise. But most of them only studied the effect of long-term endurance exercise, whereas the effect of short-term exercise remains unclear. To investigate short-term physiological adaptation induced by endurance exercise on expression of COX IV and PGC-1a mRNA in rat skeletal muscle. Twenty healthy male Wistar rats (Rattus norvegicus) aged 10-11 weeks old were used in this experiment. Rats were randomly assigned into 4 groups based on the time period of exercise: 1) control (C; n=5), 2) three days of exercise (E3; n=5), 3) six days of exercise (E6; n=5), 4) fifteen days of exercise (E15; n=5). The exercise groups were run at 20m/s for 30 minutes on the rat treadmill and the stationary control group was only placed inside treadmill with the machines turned off. On the last day of exercise, the rats were sacrificed then RNA from skeletal muscle was extracted. COX IV and PGC-1a mRNA expressions were measured by Reverse Transcriptase PCR. The results showed that there were statistically significant differences of PGC-1a mRNA expression levels in both soleus (F(3,16)=3.740, ps=0.033) and gastrocnemius (F(3,16)=3.969, pg=0.027) muscles. The COX IV mRNA expression levels in soleus (F(3,16)=3.801, ps=0.031) and gastrocnemius (F(3,16)=5.429, ps=0.009) muscles were also significantly increased. There were significant increases of PGC-1a and COX IV expressions in fifteen days of exercise group compared to control group in both muscles. Short-term endurance exercise induced mitochondrial biogenesis marker and mitochondrial activity marker by increasing the PGC-1a and COX IV mRNA expression levels in rat skeletal muscle significantly following the time periods of exercise.

scholarly journals ELEVEN WEEKS OF STRENGTH TRAINING DECREASED THE EXPRESSION OF IMMUNE-RELATED GENES IN OLDER SUBJECTS INDEPENDENT OF PROTEIN SUPPLEMENT TYPE; A RANDOMIZED CONTROLLED TRIAL

2017 ◽  
pp. 1-9
Author(s):  
G.O. Gjevestad ◽  
H. Hamarsland ◽  
T. Raastad ◽  
J.J. Christensen ◽  
A.S. Biong ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Strength Training ◽  
Mrna Expression ◽  
Protein Supplementation ◽  
Intervention Period ◽  
Expression Levels ◽  
Randomized Controlled ◽  
Native Whey ◽  
Immune Related Genes ◽  
Mrna Expression Levels

Objective: To investigate the effects of eleven weeks of strength training combined with two isocaloric protein supplements on mRNA expression levels in skeletal muscle and peripheral blood mononuclear blood cells (PBMCs). Design: A double blind randomized controlled study. Setting: The Norwegian School of Sports Sciences, Norway. Participants: Untrained, but otherwise healthy, men and women (n=20, ≥ 70 yrs). Intervention: Participants were randomly allocated to receive either milk protein or a native whey protein supplement (20 g protein, morning and afternoon) combined with a standardized strength training protocol (6-10 RM, 1-3 sets, 3 times/week) for eleven weeks. Measurements: The mRNA expression levels of immune-related genes were measured before and after the intervention period, using RT-qPCR. Cytokines were measured using ELISA. Results: PBMC mRNA expression of interleukin (IL) 6, IL8, chemokine (C-C motif) ligand (CCL) 3, and nuclear receptor subfamily (NR) 1 group H (H) member 3 decreased significantly after the intervention period, whereas the mRNA expression of toll-like receptor (TLR2) increased. In skeletal muscle, the mRNA expression of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) and PPARGC1B decreased significantly, whereas the mRNA expression of CCL2, CCL5, TLR2, TLR4 and hypoxia inducible factor 1 alpha subunit (HIF1A) increased significantly after the intervention. We found no significant differences in circulating C-reactive protein and IL6 after the intervention period. The consumption of whey and milk proteins had similar effects on mRNA expression levels after strength training in skeletal muscle as well as PBMCs. Conclusion: Eleven weeks of strength training and protein supplementation reduced the PBMC expression levels of genes involved in the immune system as well as in metabolism, underlining the close interaction between these processes. The upregulation of other immune-related genes observed in PBMCs as well as in skeletal muscle needs further investigations, but may be related to protein supplementation and training adaptations. Different protein supplementation (milk or native whey) did not differentially modulate mRNA expression after the intervention period.

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