scholarly journals Evaluation of the Double Agar Gel Immunodiffusion Test and of the Enzyme-Linked Immunosorbent Assay in the Diagnosis and Follow-Up of Patients with Chronic Pulmonary Aspergillosis

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0134841 ◽  
Author(s):  
Priscila Zacarias de Azevedo ◽  
Tatiane Fernanda Sylvestre ◽  
Ricardo de Souza Cavalcante ◽  
Lídia Raquel de Carvalho ◽  
Daniela Vanessa Moris ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pulmonary Aspergillosis ◽  
Agar Gel ◽  
Immunodiffusion Test ◽  
Chronic Pulmonary Aspergillosis

scholarly journals Serological survey of parapoxvirus infection in wild ruminants in Japan in 1996–9

2001 ◽  
Vol 126 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Y. INOSHIMA ◽  
Y. YAMAMOTO ◽  
T. TAKAHASHI ◽  
M. SHINO ◽  
A. KATSUMI ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Cervus Nippon ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Survey ◽  
Serum Samples ◽  
Agar Gel ◽  
Wild Ruminants ◽  
Immunodiffusion Test ◽  
Japanese Serow ◽  
Free Ranging

The prevalence of parapoxvirus infection was examined in free-ranging wild ruminants in Japan, Japanese serow (Capricornis crispus) and Japanese deer (Cervus nippon centralis), in 1996–9. We collected a total of 151 serum samples from 101 Japanese serows and 50 Japanese deer and tested for antibodies against parapoxvirus by an enzyme-linked immunosorbent assay and an agar gel immunodiffusion test. Overall seroprevalences among Japanese serows were 5/25 (20·0%) in 1996, 4/14 (28·6%) in 1997, 5/32 (15·6%) in 1998 and 2/30 (6·7%) in 1999, respectively. The seroprevalence increased with age but was not affected by sex. No antibodies were detected from any of 50 serum samples taken from Japanese deer. Our results in this study suggest that parapoxvirus infection is widespread among the population of Japanese serows, however, Japanese deer appear to be still free of the disease.

scholarly journals Incidence and Risk Factors of Chronic Pulmonary Aspergillosis Development during Long-Term Follow-Up after Lung Cancer Surgery

2020 ◽  
Vol 6 (4) ◽  
pp. 271
Author(s):  
Sun Shin ◽  
Bo-Guen Kim ◽  
Jiyeon Kang ◽  
Sang-Won Um ◽  
Hojoong Kim ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cumulative Incidence ◽  
Lung Cancer Surgery ◽  
Cancer Surgery ◽  
Pulmonary Aspergillosis ◽  
Factors Associated ◽  
Long Term Follow Up ◽  
Chronic Pulmonary Aspergillosis

Lung resection surgery for non-small-cell lung cancer (NSCLC) is reportedly a risk factor for developing chronic pulmonary aspergillosis (CPA). However, limited data are available regarding the development of CPA during long-term follow-up after lung cancer surgery. This study aimed to investigate the cumulative incidence and clinical factors associated with CPA development after lung cancer surgery. We retrospectively analyzed 3423 patients with NSCLC who (1) underwent surgical resection and (2) did not have CPA at the time of surgery between January 2010 and December 2013. The diagnosis of CPA was based on clinical symptoms, serological or microbiological evidences, compatible radiological findings, and exclusion of alternative diagnoses. The cumulative incidence of CPA and overall survival (OS) were estimated using the Kaplan–Meier method, and a multivariable Cox proportional hazard analysis was performed to identify factors associated with CPA development. Patients were followed-up for a median of 5.83 years with a 72.3% 5-year OS rate. Fifty-six patients developed CPA at a median of 2.68 years after surgery, with cumulative incidences of 0.4%, 1.1%, 1.6%, and 3.5% at 1, 3, 5, and 10 years, respectively. Lower body mass index (BMI), smoking, underlying interstitial lung disease, thoracotomy, development of postoperative pulmonary complications 30 days after surgery, and treatment with both chemotherapy and radiotherapy were independently associated with CPA development. The cumulative incidence of CPA after surgery was 3.5% at 10 years and showed a steadily increasing trend during long-term follow-up. Therefore, increased awareness regarding CPA development is needed especially in patients with risk factors.

scholarly journals Antibody Responses to CampylobacterInfections Determined by an Enzyme-Linked Immunosorbent Assay: 2-Year Follow-Up Study of 210 Patients

2001 ◽  
Vol 8 (2) ◽  
pp. 314-319 ◽  
Author(s):  
Mette Aagaard Strid ◽  
Jørgen Engberg ◽  
Lena Brandt Larsen ◽  
Kamilla Begtrup ◽  
Kåre Mølbak ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Serum Antibody ◽  
Large Degree ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Campylobacter Coli ◽  
Negative Results ◽  
Heat Stable Antigen

ABSTRACT An enzyme-linked immunosorbent assay (ELISA) was adapted to measure immunoglobulin G (IgG), IgM, and IgA classes of human serum antibody toCampylobacter jejuni and Campylobacter coli. Heat-stable antigen, a combination of C. jejuni serotype O:1,44 and O:53 in the ratio 1:1, was used as a coating antigen in the ELISA test. A total of 631 sera from 210 patients with verifiedCampylobacter enteritis were examined at various intervals after infection, and a control group of 164 sera were tested to determine the cut-off for negative results. With a 90th percentile of specificity, IgG, IgM, and IgA showed a sensitivity of 71, 60, and 80%, respectively. By combining all three antibody classes, the sensitivity was 92% within 35 days after infection, whereas within 90 days after infection, a combined sensitivity of 90% was found (IgG 68%, IgM 52%, and IgA 76%). At follow-up of the patients, IgG antibodies were elevated 4.5 months after infection but exhibited a large degree of variation in antibody decay profiles. IgA and IgM antibodies were elevated during the acute phase of infection (up to 2 months from onset of infection). The antibody response did not depend on Campylobacter species or C. jejuniserotype, with the important exception of response to C. jejuni O:19, the serotype most frequently associated with Guillain-Barré syndrome. All of the patients infected with this serotype had higher levels of both IgM (P = 0.006) and IgA (P = 0.06) compared with other C. jejuni and C. coli serotypes.

scholarly journals Validation of an Enzyme-Linked Immunosorbent Assay That Detects Histoplasma capsulatum Antigenuria in Colombian Patients with AIDS for Diagnosis and Follow-Up during Therapy

2014 ◽  
Vol 21 (9) ◽  
pp. 1364-1368 ◽  
Author(s):  
Diego H. Caceres ◽  
Christina M. Scheel ◽  
Ángela M. Tobón ◽  
Angela Ahlquist Cleveland ◽  
Ángela Restrepo ◽  
...  
Keyword(s):  
Clinical Improvement ◽  
Immunosorbent Assay ◽  
Histoplasma Capsulatum ◽  
Response To Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Test ◽  
Content Type ◽  
Antigen Capture

ABSTRACTWe validated an antigen capture enzyme-linked immunosorbent assay (ELISA) in Colombian persons with AIDS and proven histoplasmosis and evaluated the correlation between antigenuria and clinical improvement during follow-up. The sensitivity of theHistoplasma capsulatumELISA was 86%, and the overall specificity was 94%. The antigen test successfully monitored the response to therapy.

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