scholarly journals A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0148989 ◽  
Author(s):  
Kazuki Moriguchi ◽  
Shinji Yamamoto ◽  
Yuta Ohmine ◽  
Katsunori Suzuki
Keyword(s):  
Escherichia Coli ◽  
Transformation Method ◽  
Conjugal Transfer ◽  
Transfer System ◽  
Yeast Transformation ◽  
Two Cultures

Conjugal transfer of cosmid DNA from Escherichia coli to Saccharopolyspora spinosa: effects of chromosomal insertions on macrolide A83543 production

Gene ◽  
1994 ◽  
Vol 146 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Patti Matsushima ◽  
M.Chris Broughton ◽  
Jan R. Turner ◽  
Richard H. Baltz
Keyword(s):  
Escherichia Coli ◽  
Conjugal Transfer ◽  
Saccharopolyspora Spinosa

Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.

Gene ◽  
1992 ◽  
Vol 116 (1) ◽  
pp. 43-49 ◽  
Author(s):  
M. Bierman ◽  
R. Logan ◽  
K. O'Brien ◽  
E.T. Seno ◽  
R. Nagaraja Rao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Conjugal Transfer ◽  
Cloning Vectors ◽  
Streptomyces Spp

Transformation of Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 by CaCl2 method

2010 ◽  
Vol 56 (1) ◽  
pp. 74-76 ◽  
Author(s):  
Urmi Patel ◽  
Sarika Sinha
Keyword(s):  
Escherichia Coli ◽  
Optical Density ◽  
Sinorhizobium Meliloti ◽  
Transformation Efficiency ◽  
Binary Vector ◽  
Transformation Method ◽  
Mesorhizobium Ciceri ◽  
Competent Cells

The CaCl2 method, commonly used for transformation of Escherichia coli, was modified and used to develop a simpler and easier transformation method for Rhizobia sp. Two species of Rhizobia, Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620, were transformed with the 13.2 kb binary vector pGA482. At an optical density of 0.4, the transformation efficiencies in Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 were 104 and 103, respectively. Competent cells of Sinorhizobium meliloti MTCC 100 were prepared at different growth intervals and transformed by the same vector. A maximum transformation efficiency of 104 was achieved at an optical density of 0.5.

scholarly journals An improved yeast transformation method for the generation of very large human antibody libraries

2010 ◽  
Vol 23 (4) ◽  
pp. 155-159 ◽  
Author(s):  
Lorenzo Benatuil ◽  
Jennifer M. Perez ◽  
Jonathan Belk ◽  
Chung-Ming Hsieh
Keyword(s):  
Transformation Method ◽  
Human Antibody ◽  
Yeast Transformation ◽  
Antibody Libraries
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