scholarly journals Gene Expression Dynamics Accompanying the Sponge Thermal Stress Response

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0165368 ◽  
Author(s):  
Christine Guzman ◽  
Cecilia Conaco
Keyword(s):  
Gene Expression ◽  
Thermal Stress ◽  
Stress Response ◽  
Gene Expression Dynamics

scholarly journals Global gene expression patterns in response to white patch syndrome: Disentangling symbiont loss from the thermal stress response in reef-building coral

2019 ◽  
Author(s):  
Carly D. Kenkel ◽  
Veronique J.L. Mocellin ◽  
Line K. Bay
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Thermal Stress ◽  
Stress Response ◽  
Meta Analysis ◽  
Expression Patterns ◽  
Global Gene Expression ◽  
Host Protein ◽  
Gene Expression Patterns ◽  
White Patch

AbstractThe mechanisms resulting in the breakdown of the coral symbiosis once the process of bleaching has been initiated remain unclear. Distinguishing symbiont loss from the abiotic stress response may shed light on the cellular and molecular pathways involved in each process. This study examined physiological changes and global gene expression patterns associated with white patch syndrome (WPS) in P. lobata, which manifests in localized bleaching independent of thermal stress. In addition, a meta-analysis of global gene expression studies in other corals and anemones was used to contrast differential regulation as a result of abiotic stress from expression patterns correlated with symbiotic state. Symbiont density, chlorophyll a content, holobiont productivity, instant calcification rate, and total host protein content were uniformly reduced in WPS relative to healthy tissue. While expression patterns associated with WPS were secondary to fixed effects of source colony, specific functional enrichments suggest that the viral infection putatively giving rise to this condition affects symbiont rather than host cells. The meta-analysis revealed that expression patterns in WPS-affected tissues were significantly correlated with prior studies examining short-term thermal stress responses. This correlation was independent of symbiotic state, as the strongest correlations were found between WPS adults and both symbiotic adult and aposymbiotic coral larvae experiencing thermal stress, suggesting that the majority of expression changes reflect a non-specific stress response. Across studies, the magnitude and direction of expression change among particular functional enrichments suggests unique responses to stressor duration, and highlights unique responses to bleaching in an anemone model which engages in a non-obligate symbiosis.

scholarly journals Local interactions lead to spatially correlated gene expression levels in bacterial groups

2017 ◽  
Author(s):  
Simon van Vliet ◽  
Alma Dal Co ◽  
Annina R. Winkler ◽  
Stefanie Spriewald ◽  
Bärbel Stecher ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Regulatory Networks ◽  
Toxin Production ◽  
Spatial Correlations ◽  
Spatially Correlated ◽  
Spatial Gradients ◽  
Gene Expression Dynamics ◽  
A Cell ◽  
State Of The Environment

AbstractMany bacteria live in spatially structured assemblies where the microenvironment of a cell is shaped by the activities of its neighbors. Bacteria regulate their gene expression based on the inferred state of the environment. This raises the question whether the phenotypes of neighboring cells can become correlated through interactions via the shared microenvironment. Here, we addressed this question by following gene expression dynamics in Escherichia coli microcolonies. We observed strong spatial correlations in the expression dynamics for pathways involved in toxin production, SOS-stress response, and metabolism. These correlations can partly be explained by a combination of shared lineage history and spatial gradients in the colony. Interestingly, we also found evidence for cell-cell interactions in SOS-stress response, methionine biosynthesis and overall metabolic activity. Together our data suggests that intercellular feedbacks can couple the phenotypes of neighboring cells, raising the question whether gene-regulatory networks have evolved to spatially organize biological functions.

scholarly journals Stress-induced differential gene expression in cardiac tissue

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana Elisa T. S. de Carvalho ◽  
Marco A. Cordeiro ◽  
Luana S. Rodrigues ◽  
Daniela Ortolani ◽  
Regina C. Spadari
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Left Ventricle ◽  
Differential Gene Expression ◽  
Cardiac Tissue ◽  
Stressful Situation ◽  
Adaptive Mechanism ◽  
Number Of Genes ◽  
Differential Gene ◽  
Shock Stress

AbstractThe stress response is adaptive and aims to guarantee survival. However, the persistence of a stressor can culminate in pathology. Catecholamines released as part of the stress response over activate beta adrenoceptors (β-AR) in the heart. Whether and how stress affects the expression of components of the intracellular environment in the heart is still, however, unknown. This paper used microarray to analyze the gene expression in the left ventricle wall of rats submitted to foot shock stress, treated or not treated with the selective β2-AR antagonist ICI118,551 (ICI), compared to those of non-stressed rats also treated or not with ICI, respectively. The main findings were that stress induces changes in gene expression in the heart and that β2-AR plays a role in this process. The vast majority of genes disregulated by stress were exclusive for only one of the comparisons, indicating that, in the same stressful situation, the profile of gene expression in the heart is substantially different when the β2-AR is active or when it is blocked. Stress induced alterations in the expression of such a large number of genes seems to be part of stress-induced adaptive mechanism.

scholarly journals Deuterium Oxide (D2O) Induces Early Stress Response Gene Expression and Impairs Growth and Metastasis of Experimental Malignant Melanoma

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 605
Author(s):  
Jana Jandova ◽  
Anh B. Hua ◽  
Jocelyn Fimbres ◽  
Georg T. Wondrak
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Malignant Melanoma ◽  
Tumor Growth ◽  
Deuterium Oxide ◽  
Human Melanoma ◽  
Pharmacological Intervention ◽  
Melanoma Metastasis ◽  
Response Gene ◽  
Stress Response Gene

There are two stable isotopes of hydrogen, protium (1H) and deuterium (2H; D). Cellular stress response dysregulation in cancer represents both a major pathological driving force and a promising therapeutic target, but the molecular consequences and potential therapeutic impact of deuterium (2H)-stress on cancer cells remain largely unexplored. We have examined the anti-proliferative and apoptogenic effects of deuterium oxide (D2O; ‘heavy water’) together with stress response gene expression profiling in panels of malignant melanoma (A375V600E, A375NRAS, G361, LOX-IMVI), and pancreatic ductal adenocarcinoma (PANC-1, Capan-2, or MIA PaCa-2) cells with inclusion of human diploid Hs27 skin fibroblasts. Moreover, we have examined the efficacy of D2O-based pharmacological intervention in murine models of human melanoma tumor growth and metastasis. D2O-induction of apoptosis was substantiated by AV-PI flow cytometry, immunodetection of PARP-1, and pro-caspase 3 cleavage, and rescue by pan-caspase inhibition. Differential array analysis revealed early modulation of stress response gene expression in both A375 melanoma and PANC-1 adenocarcinoma cells elicited by D2O (90%; ≤6 h) (upregulated: CDKN1A, DDIT3, EGR1, GADD45A, HMOX1, NFKBIA, or SOD2 (up to 9-fold; p < 0.01)) confirmed by independent RT-qPCR analysis. Immunoblot analysis revealed rapid onset of D2O-induced stress response phospho-protein activation (p-ERK, p-JNK, p-eIF2α, or p-H2AX) or attenuation (p-AKT). Feasibility of D2O-based chemotherapeutic intervention (drinking water (30% w/w)) was demonstrated in a severe combined immunodeficiency (SCID) mouse melanoma metastasis model using luciferase-expressing A375-Luc2 cells. Lung tumor burden (visualized by bioluminescence imaging) was attenuated by D2O, and inhibition of invasiveness was also confirmed in an in vitro Matrigel transwell invasion assay. D2O supplementation also suppressed tumor growth in a murine xenograft model of human melanoma, and median survival was significantly increased without causing adverse effects. These data demonstrate for the first time that systemic D2O administration impairs growth and metastasis of malignant melanoma through the pharmacological induction of deuterium (2H)-stress.

Potential role of specific microRNAs in the regulation of thermal stress response in livestock

2021 ◽  
Vol 96 ◽  
pp. 102859
Author(s):  
Sayed Haidar Abbas Raza ◽  
Sameh A. Abdelnour ◽  
Aya I.M. Dhshan ◽  
Abdallah A. Hassanin ◽  
Ahmed E. Noreldin ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Stress Response ◽  
Potential Role
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