scholarly journals Comparative sensitivity of the test with tuberculosis recombinant allergen, containing ESAT6-CFP10 protein, and Mantoux test with 2 TU PPD-L in newly diagnosed tuberculosis children and adolescents in Moscow

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208705 ◽  
Author(s):  
Liudmila Slogotskaya ◽  
Elena Bogorodskaya ◽  
Diana Ivanova ◽  
Tatiana Sevostyanova
Keyword(s):  
Children And Adolescents ◽  
Mantoux Test ◽  
Recombinant Allergen ◽  
Newly Diagnosed ◽  
Comparative Sensitivity

scholarly journals Comparative sensitivity of the test with tuberculosis recombinant allergen, containing ESAT6-CFP10 protein, and Mantoux test with 2 TU PPD-L in newly diagnosed tuberculosis children and adolescents in Moscow

2018 ◽  
Author(s):  
Liudmila Slogotskaya ◽  
Elena Bogorodskaya ◽  
Diana Ivanova ◽  
Tatiana Sevostyanova
Keyword(s):  
Children And Adolescents ◽  
Mass Screening ◽  
Skin Testing ◽  
High Sensitivity ◽  
High Specificity ◽  
Cost Effective ◽  
Mantoux Test ◽  
Recombinant Allergen ◽  
Newly Diagnosed ◽  
Comparative Sensitivity

AbstractBackground. A group of Russian scientists has developed Diaskintest, which comprises Mycobacterium tuberculosis-specific recombinant proteins CFP10-ESAT6, for skin testing (0.2 µg/0.1 ml).Study purpose: to evaluate the comparative sensitivity of TST with 2 TU PPD-L and a skin test with tuberculous recombinant allergen (Diaskintest) containing the ESAT6-CFP10 protein in children and adolescents with newly diagnosed respiratory tuberculosis during mass screening in the primary medical service in Moscow, 2013–2016.Materials and methods. The trial was a comprehensive retrospective group study of children and adolescents diagnosed in Moscow with respiratory tuberculosis in 2013–2016, aged 0 to 17 years inclusive. From 441 patients selected for analysis 408 patients had both tests (TST with 2 TU PPD-L and Diaskintest) performed, in 193 patients both tests were given simultaneously, of them 162 patients were BCG-vaccinated.Results. Comparative results of both tests in 408 patients with tuberculosis: at cut-off 5 mm, both tests has similar sensitivity: Diaskintest 98.3 % (95 % CI 97.0–99.6 %), TST 98.0 % (95 % CI 96.7–99.4 %), at cut-off 10 mm, the sensitivity decreases for both tests: Diaskintest 90.0 % (95 % CI 87.0–93.0 %), TST 88.7 % (95 % CI 85.6–91.9 %), but at cut-off 15 mm, the decrease in sensitivity is statistically significant: for Diaskintest 61.5 % (95 % CI 56.7–66.3 %), and for TST 46.3 % (95 % CI 41.4–51.3 %), p <0.0001.The results of simultaneous setting of tests on different hands in 193 people (including 162 BCG-vaccinated), do not differ from the results for 408 people.The correlation between the results of Diaskintest and TST was significant in all groups.Conclusion. In children and adolescents with respiratory tuberculosis, Diaskintest of 0.2 µg/ml and the Mantoux test with 2 TU PPD-L have high sensitivity (98%) at a cut-off of 5 mm; however, at cut-off 15 mm sensitivity is significantly reduced, and the decrease is more pronounced in the Mantoux test. The advantage of Diaskintest is that, unlike the Mantoux test, it has high specificity under the conditions of mass BCG vaccination. The test is cost-effective, simple to carry out, and can be used in mass screening.

scholarly journals FORMATION OF RISK GROUPS FOR TUBERCULOSIS DISEASE IN VARIOUS IMMUNOLOGICAL METHODS OF THE EXAMINATION OF CHILDREN POPULATION

2019 ◽  
Vol 20 (4) ◽  
pp. 207-213 ◽  
Author(s):  
Liudmila V. Slogotskaya ◽  
E. M. Bogorodskaya ◽  
O. Yu. Senchikhina ◽  
G. V. Nikitina ◽  
D. A. Kudlay
Keyword(s):  
Children And Adolescents ◽  
Positive Reaction ◽  
Primary Infection ◽  
Risk Group ◽  
Risk Groups ◽  
Mantoux Test ◽  
Immunological Methods ◽  
High Threshold ◽  
Recombinant Allergen ◽  
Age Related

When performing mass tuberculin diagnostics in Moscow with coverage of more than 97% of children, the authors evaluated the effectiveness of the formation of risk groups for tuberculosis in children and adolescents with the use of immunological methods such as Mantoux test with 2 TE PPD-L and a test with an tuberculosis recombinant allergen (TRA). There was formed a risk group of cases with altered tuberculin sensitivity (group VI) in which all children underwent a skin test with ATP (Diaskintest preparation) at a dose of 0.2 μg in 0.1 ml. and in the presence of a positive reaction to this test, computed tomography was performed. The low specificity of tuberculin diagnostics in conditions of mass vaccination of children with BCG was found to lead to the fact that the frequency of positive reactions to the Mantoux test is determined primarily by postvaccinal allergy. Accumulation of cases of primary infection, hyperergia and increased reaction is less than 1% per year, and the frequency of positive reactions in the population is above 75%. It was also revealed that only 8.7-11.2% of children and adolescents with a positive reaction to the Mantoux test are examined in an anti-TB dispensary and less than 1% - are to be accounted by phthisiatricians at the risk group. In risk groups selected by the Mantoux sample, in cases with a high threshold of positive response to this test (17 mm and more), the frequency of positive responses to the TRA test is 53.3% in children and 81.4% in adolescents. With age, the proportion of primary infection among all tuberculin-positive Mantoux tests declines from 0.67% in children to 0.06% in adolescents, and the age-related positive responses for the RTA test in risk groups increase from 16.3 to 52.6% respectively. The implementation of the preventive therapy only for children with a high risk of the development of the disease (with a positive RTA test) increases the effectiveness of this therapy and excludes its unreasonable realization.

POSSIBILITIES OF A LABORATORY TEST BASED ON THE DETERMINATION OF THE T-CELL RESPONSE TO STIMULATION WITH SPECIFIC CFP10 AND ESAT6 ANTIGENS IN THE DETECTION OF TUBERCULOSIS INFECTION IN CHILDREN AND ADOLESCENTS

2021 ◽  
Vol 100 (4) ◽  
pp. 128-132
Author(s):  
R.H. Fatykhova ◽  
◽  
L.А. Bаryshnikovа ◽  
D.A. Kudlay ◽  
A.P. Alekseev ◽  
...  
Keyword(s):  
Laboratory Test ◽  
Children And Adolescents ◽  
Retrospective Cohort ◽  
T Cell Response ◽  
Tuberculosis Infection ◽  
Mantoux Test ◽  
History Of ◽  
Positive Results ◽  
Reverse Development

Objective of the study: to explore the possibilities of T-SPOT.TB testing to detect tuberculosis infection (TI) in children and adolescents. Materials and methods of research: the design – a multicenter retrospective cohort noncomparative uncontrolled study. The results of examination of children aged 1 to 17 years using the T-SPOT.TB test (n=1565): 774 boys (49,5%), 791 girls (50,5%). The median age of children is 8 [5; 13] years. Results: the rate of positive T-SPOT.TB tests was 7,2% (n=113). Further examination and in-depth examination of children with positive results (n=28), in every 4th case, a history of contact with a tuberculosis (TB) patient was established, every 4th child had previously been under treatment with phthisiatrician. The coincidence of positive results of the T-SPOT.TB test with the Mantoux test – in 87%, with the test with a recombinant tuberculosis allergen – in 100% of cases. Local TB in the phase of reverse development was detected in one child (4%), active TB – in 2 (7%). Conclusion: the T-SPOT.TB test is an alternative method for screening children for TI.

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