High-throughput sequencing analysis of the microbial community in coastal intensive mariculture systems

The intestinal flora is a micro-ecosystem that is closely linked to the overall health of the host. We examined the diversity and abundance of intestinal microorganisms in mice following the administration of andrographolide, a component of the Chinese medical herb Andrographis paniculata. Administration of andrographolide produces multiple beneficial effects including anti-inflammatory, antiviral and antibacterial effects but whether it directly influences the gut microbiota is not known. This study investigated whether the oral administration of andrographolide influences the intestinal microbiota and was compared with amoxicillin treatment as a positive control and water only as a negative control. We examined 21 cecal samples and conducted a high-throughput sequencing analysis based on V3-V4 variable region of the 16S rDNA genes. We found that the diversity and abundance of mouse gut microbiota decreased in direct proportion with the amoxicillin dose whereas andrographolide administration did not affect intestinal microbial community structure. The composition of intestinal microbes following andrographolide treatment was dominated by the Firmicutes while Bacteroidetes dominated the amoxicillin treatment group compared with the negative controls. Specifically, the f__Lachnospiraceae_ Unclassified, Lachnospiraceae_ NK4A136_group and Ruminococcaceae_ UCG-014 were enriched with andrographolide administration while Bacteroides, Klebsiella and Escherichia-Shigella significantly increased in the amoxicillin test groups. Amoxicillin administration altered the microbial community composition and structure by increasing the proportion of pathogenic to beneficial bacteria whereas andrographolide administration led to increases in the proportions and abundance of beneficial bacteria. This study provides a theoretical basis for finding alternatives to antibiotics to decrease bacterial resistance and restore intestinal floral imbalances.

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Dynamic characteristics of immobilized microorganisms for remediation of nitrogen-contaminated groundwater and high-throughput sequencing analysis of the microbial community

Environmental Pollution ◽

10.1016/j.envpol.2020.114875 ◽

2020 ◽

Vol 267 ◽

pp. 114875

Author(s):

Shuo Li ◽

Mingxiang Yang ◽

Hao Wang ◽

Yong Zhao

Keyword(s):

Microbial Community ◽

High Throughput ◽

Dynamic Characteristics ◽

High Throughput Sequencing ◽

Contaminated Groundwater ◽

Sequencing Analysis ◽

Immobilized Microorganisms

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Chromosome 22q11.21 and 11p15.4 microdeletions confirmed by high‐throughput sequencing analysis in one patient with asymmetric cry syndrome: Case report and review of the literature

Clinical Case Reports ◽

10.1002/ccr3.4072 ◽

2021 ◽

Author(s):

Yonghong Pang ◽

Yang Yu ◽

Xiaoyi Deng ◽

Qian Liu ◽

Junmei Yan ◽

...

Keyword(s):

Case Report ◽

High Throughput ◽

High Throughput Sequencing ◽

Sequencing Analysis ◽

Review Of The Literature

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High-Throughput Sequencing Analysis of the Actinobacterial Spatial Diversity in Moonmilk Deposits

Antibiotics ◽

10.3390/antibiotics7020027 ◽

2018 ◽

Vol 7 (2) ◽

pp. 27 ◽

Cited By ~ 8

Author(s):

Marta Maciejewska ◽

Magdalena Całusińska ◽

Luc Cornet ◽

Delphine Adam ◽

Igor Pessi ◽

...

Keyword(s):

High Throughput ◽

High Throughput Sequencing ◽

Spatial Diversity ◽

Sequencing Analysis

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Diversity of microbial communities in hot springs of Sri Lanka as revealed by 16S rRNA gene high-throughput sequencing analysis

Gene ◽

10.1016/j.gene.2021.146103 ◽

2021 ◽

pp. 146103

Author(s):

Dilini Sadeepa ◽

Kosala Sirisena ◽

Pathmalal M. Manage

Keyword(s):

Sri Lanka ◽

16S Rrna ◽

16S Rrna Gene ◽

Microbial Communities ◽

High Throughput ◽

High Throughput Sequencing ◽

Hot Springs ◽

Rrna Gene ◽

Sequencing Analysis

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Analysis of Microbial Community Structure and Volatile Compounds in the Pit Mud Used for Manufacturing Taorong-type Baijiu Based on High-Throughput Sequencing

10.21203/rs.3.rs-1097837/v1 ◽

2021 ◽

Author(s):

Yanbo Liu ◽

Mengxiao Sun ◽

Pei Hou ◽

Wenya Wang ◽

Xiangkun Shen ◽

...

Keyword(s):

Community Structure ◽

Microbial Community ◽

Qualitative Analysis ◽

Volatile Compounds ◽

High Throughput ◽

Microbial Community Structure ◽

High Throughput Sequencing ◽

Solid Phase ◽

Gas Chromatography Mass Spectrometry ◽

Pit Mud

Abstract In this study, the pit mud used in manufacturing Taorong-type Baijiu was collected from the upper, middle, lower and bottom layers of pits in Henan Yangshao Liquor Co., LTD. Besides, high-throughput sequencing (HTS) technology was adopted to analyze the microbial community structure of the pit mud. In addition, the volatile compounds in the pit mud were subjected to preliminarily qualitative analysis through headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The results of HTS demonstrated that there were 5, 3, 5 and 5 dominant bacterial phyla (including 11, 11, 9 and 8 dominant bacterial genera) and 3, 3, 3 and 3 dominant fungal phyla (including 4, 7, 7 and 5 dominant fungal genera) in the pit mud from F-S (upper), G-Z (middle), H-X (lower) and I-D (bottom), respectively. The qualitative analysis results of volatile compounds demonstrated that a total of 78 kinds of volatile compounds were detected in the pit mud, including 46, 45, 39 and 49 kinds in the pit mud from F-S, G-Z, H-X and I-D, respectively. Ester and acid were the two main components in the pit mud. Meanwhile, the correlation between microorganisms and main volatile compounds in the pit mud was analyzed. Moreover, Lentimicrobium, Syner-01 and Blvii28_wastewater-sludge group were found for the first time in the pit mud used for manufacturing Taorong-type Baijiu. The findings of this study could provide a theoretical foundation for improving the quality of pit mud and the flavor of Taorong-type Baijiu.

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