scholarly journals Stress hormones or general well-being are not altered in immune-deficient mice lacking either T- and B- lymphocytes or Interferon gamma signaling if kept under specific pathogen free housing conditions

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0239231
Author(s):  
Sarah Jeuthe ◽  
Josephine Kemna ◽  
Christoph Philipp Kemna ◽  
Dario Zocholl ◽  
Robert Klopfleisch ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Interferon Gamma ◽  
Stress Hormones ◽  
Well Being ◽  
T And B Lymphocytes ◽  
Housing Conditions ◽  
Specific Pathogen Free ◽  
Specific Pathogen ◽  
Deficient Mice

scholarly journals Donor Microbiota Composition and Housing Affect Recapitulation of Obese Phenotypes in a Human Microbiota-Associated Murine Model

2021 ◽  
Vol 11 ◽  
Author(s):  
Thomas Kaiser ◽  
Harika Nalluri ◽  
Zhigang Zhu ◽  
Christopher Staley
Keyword(s):  
Weight Gain ◽  
Insulin Sensitivity ◽  
Intestinal Microbiota ◽  
Microbiota Composition ◽  
Housing Conditions ◽  
Specific Pathogen Free ◽  
Human Microbiota ◽  
Specific Effects ◽  
Specific Pathogen

Human microbiota-associated (HMA) mouse models offer a valuable approach to study the role of intestinal microbiota in the development of obesity. In this study, we used an HMA model to evaluate whether engraftment of human obese or lean microbiota, from each of three donors, could recapitulate host phenotypes under conventional and specific-pathogen-free housing. Microbiota engraftment was correlated with donor relative abundances of the class Bacteroidia (Spearman’s ρ = 0.73, P ≤ 0.001), and one obese donor resulted in significant weight gain (P ≤ 0.003) and compromised insulin sensitivity under conventional housing. SPF housing partially blunted phenotypic response. Results of this study indicate that our HMA model partially recapitulates obese phenotypes under conventional housing and highlights a need to consider donor-specific effects as well as housing conditions when studying the role of the microbiota in obesity.

Relationship among T- and B-lymphocytes of the peripheral blood and thyroid tissue in patients with Graves' disease

2019 ◽  
Author(s):  
Margarita A Dudina ◽  
Andrey A Savchenko ◽  
Sergey A Dogadin ◽  
Alexandr G Borisov ◽  
Igor V Kudryavcev ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Peripheral Blood ◽  
Thyroid Tissue ◽  
T And B Lymphocytes ◽  
Graves Disease

Laboratory Animal Research Centre (Larc) “A Specific Pathogen Free Rodent Facility” - Covid – 19 Pandemic Response Plan

2020 ◽  
Author(s):  
Muralitharan Shanmugakonar ◽  
Vijay Kanth Govindharajan ◽  
Kavitha Varadharajan ◽  
Hamda Al-Naemi
Keyword(s):  
Laboratory Animal ◽  
Animal Research ◽  
Research Centre ◽  
Functional Requirement ◽  
Specific Pathogen Free ◽  
Specific Pathogen ◽  
Response Plan ◽  
Pandemic Response ◽  
Operational Plan

Laboratory Animal Research Centre (LARC) has developed an early emergency operational plan for COVID-19 pandemic situation. Biosafety and biosecurity measures were planned and implemented ahead of time to check the functional requirement to prevent the infection. Identified necessary support for IT, transport, procurement, finance, admin and research to make the operations remotely and successfully.

scholarly journals Transplantable progenitors of natural killer cells are distinct from those of T and B lymphocytes.

1986 ◽  
Vol 83 (10) ◽  
pp. 3427-3431 ◽  
Author(s):  
J. Hackett ◽  
G. C. Bosma ◽  
M. J. Bosma ◽  
M. Bennett ◽  
V. Kumar
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
B Lymphocytes ◽  
T And B Lymphocytes ◽  
Killer Cells

scholarly journals Curcumin reduces enteric isoprostane 8-iso-PGF2α and prostaglandin GF2α in specific pathogen-free Leghorn chickens challenged with Eimeria maxima

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Victor M. Petrone-Garcia ◽  
Raquel Lopez-Arellano ◽  
Gabriela Rodríguez Patiño ◽  
Miriam Aide Castillo Rodríguez ◽  
Daniel Hernandez-Patlan ◽  
...  
Keyword(s):  
Pilot Study ◽  
Broiler Chickens ◽  
Solid Phase ◽  
Oxidation Products ◽  
Specific Pathogen Free ◽  
Post Challenge ◽  
Eimeria Maxima ◽  
Lipid Oxidation Products ◽  
Challenge Control ◽  
Specific Pathogen

AbstractThe purpose of this pilot study was to evaluate and determine the concentration of prostaglandin GF2α (PGF2α) and isoprostane 8‐iso‐PGF2α in plasma and intestine of specific pathogen-free (SPF) Leghorn chickens challenged with Eimeria maxima, with or without dietary supplementation of curcumin using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Eighty 1-day-old male SPF chickens were randomly allocated to one of four groups with four replicates (n = 5 chickens/replicate). Groups consisted of: (1) Control (no challenge), (2) Curcumin (no challenge), (3) Eimeria maxima (challenge), and (4) Eimeria maxima (challenge) + curcumin. At day 28 of age, all chickens in the challenge groups were orally gavaged with 40,000 sporulated E. maxima oocysts. No significant differences (P > 0.05) were observed in the groups regardless of the treatment or challenge with E. maxima. Enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days and 9 days post-challenge were significantly increased (P < 0.01) compared to the non-challenge control chickens. Interestingly, the enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days post-challenge were significantly reduced in chickens fed curcumin, compared to control chickens challenge with E. maxima. At 9 days post-challenge, only levels of isoprostane 8‐iso‐PGF2α in the enteric samples were significantly reduced in chickens challenged with E. maxima supplemented with curcumin, compared with E. maxima challenge chickens. No differences of isoprostane 8‐iso‐PGF2α or PGF2α were observed in plasma at both days of evaluation. Similarly, no significant differences were observed between the challenge control or chickens challenge with E. maxima and supplemented with curcumin at both times of evaluation. The results of this pilot study suggests that the antioxidant anti-inflammatory properties of curcumin reduced the oxidative damage and subsequent intestinal mucosal over-production of lipid oxidation products. Further studies to confirm and extend these results in broiler chickens are required.

scholarly journals An approach to assess stress in response to drive hunts using cortisol levels of wild boar (Sus scrofa)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Justine Güldenpfennig ◽  
Marion Schmicke ◽  
Martina Hoedemaker ◽  
Ursula Siebert ◽  
Oliver Keuling
Keyword(s):  
Wild Boar ◽  
Stress Hormones ◽  
Age Groups ◽  
Group Living ◽  
Well Being ◽  
Lower Saxony ◽  
Cortisol Levels ◽  
Negative Impacts ◽  
The Impact ◽  
Effect Of Age

AbstractHunting can easily be linked to stress in wildlife. Drive hunts performed two to three times in one area during the respective hunting period, are thought to decrease the pressure hunting places on wildlife. Nevertheless, the expression of cortisol—one of the main mammalian stress hormones—is considered to have negative impacts on animals’ well-being if expressed excessively, which may occur during some (especially repeated) hunting events. We explored the effect of drive hunts on cortisol levels in wild boar in Lower Saxony, Germany, compared these cortisol levels to reference values given by a similar study, and investigated the effect of age, sex, and pregnancy. Blood collected from wild boar shot on drive hunts was analysed using a radioimmunoassay. As expected, we observed elevated cortisol levels in all samples, however, we still found significant differences between age groups and sexes, as well as an influence of pregnancy on cortisol levels. The effect of drive hunts on cortisol levels appears to be weaker than predicted, while the effects of other variables, such as sex, are distinct. Only half of the evaluated samples showed explicitly increased cortisol levels and no significant differences were found between sampling months and locations. Group living animals and pregnant females showed significantly higher cortisol levels. The impact of hunting is measurable but is masked by natural effects such as pregnancy. Thus, we need more information on stress levels in game species.

scholarly journals Attenuated fibrosis in specific pathogen‐free microbiota in experimental cholestasis‐ and toxin‐induced liver injury

2019 ◽  
Vol 33 (11) ◽  
pp. 12464-12476 ◽  
Author(s):  
Sheida Moghadamrad ◽  
Mohsin Hassan ◽  
Kathy D. McCoy ◽  
Jorum Kirundi ◽  
Philipp Kellmann ◽  
...  
Keyword(s):  
Liver Injury ◽  
Specific Pathogen Free ◽  
Specific Pathogen ◽  
Experimental Cholestasis

Monoclonal antibodies that distinguish bovine T and B lymphocytes

1985 ◽  
Vol 9 (1) ◽  
pp. 87-102 ◽  
Author(s):  
Harris A. Lewin ◽  
William C. Davis ◽  
Domenico Bernoco
Keyword(s):  
Monoclonal Antibodies ◽  
B Lymphocytes ◽  
T And B Lymphocytes
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