scholarly journals Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0246982
Author(s):  
Carla B. Schubiger ◽  
M. Elena Gorman ◽  
Jennifer L. Johns ◽  
Mary R. Arkoosh ◽  
Joseph P. Dietrich
Keyword(s):  
Blood Cell ◽  
Fish Species ◽  
Cell Morphology ◽  
Blood Cells ◽  
Reference Intervals ◽  
Assessment Tools ◽  
Aquatic Animal ◽  
Plasma Biochemistry ◽  
Cell Counts ◽  
Blood Cell Morphology

Plasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (RI) for their plasma biochemistry and hematology along with reference photomicrographs of blood cells in healthy, fasted sablefish. Blood cell morphology can differ between fish species. In addition, blood cell counts and blood chemistry can vary between fish species, demographics, water conditions, seasons, diets, and culture systems, which precludes the use of RI’s from other fish species. For this study, blood was collected for plasma biochemistry and hematology analysis between June 20 and July 18, 2019, from healthy, yearling sablefish, hatched and reared in captivity on a commercial diet. Overnight fast of 16–18 hours did not sufficiently reduce lipids in the blood, which led to visible lipemia and frequent rupture of blood cells during analysis. Therefore, sablefish should be fasted for 24 to 36 hours before blood is collected to reduce hematology artifacts or possible reagent interference in plasma biochemistry analysis. Lymphocytes were the most dominant leukocytes (98%), while eosinophils were rare, and basophils were not detected in sablefish. Neutrophils were very large cells with Döhle bodies. In mammals and avian species, Döhle bodies are usually signs of toxic change from inflammation, but no such association was found in these fish. In conclusion, lipemia can interfere with sablefish blood analysis, and available removal methods should be evaluated as fasting for up to 36 h might not always be feasible. Also, more studies are required to establish RI for different developmental stages and rearing conditions.

scholarly journals Sablefish (Anoplopoma fimbria) plasma biochemistry and hematology reference intervals including blood cell morphology

2021 ◽  
Author(s):  
Carla B. Schubiger ◽  
M. Elena Gorman ◽  
Jennifer L. Johns ◽  
Mary R. Arkoosh ◽  
Joseph P. Dietrich
Keyword(s):  
Blood Cell ◽  
Blood Cells ◽  
Animal Health ◽  
Reference Intervals ◽  
Assessment Tools ◽  
Aquatic Animal ◽  
Plasma Biochemistry ◽  
Anoplopoma Fimbria ◽  
Commercial Diet ◽  
Cell Counts

AbstractPlasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (RI) for their plasma biochemistry and hematology along with reference photomicrographs of blood cells in healthy, fasted sablefish. Blood cell counts and morphology can vary between species, precluding the use of RI’s from other fish species for use in sablefish. Blood was collected for plasma biochemistry and hematology analysis between June 20 and July 18, 2019, from healthy, yearling sablefish, hatched and reared in captivity on a commercial diet. Overnight fast of 16-18 hours did not sufficiently reduce lipids in the blood, which lead to visible lipemia and frequent rupture of blood cells during analysis. Therefore, sablefish should be fasted for 24 to 36 hours before blood is collected to reduce hematology artifacts or possible reagent interference in plasma biochemistry analysis. RI’s may also be influenced by feed, demographics, and production systems, warranting more studies on this topic. In sablefish, lymphocytes are the dominant leukocytes (98%), while eosinophils are rare, and basophils were not detected. Neutrophils are very large cells with Döhle bodies. These bodies are usually signs of toxic changes, but no such association was found in these fish.

Hematologic and biochemical reference intervals and blood cell morphology in South American pit vipers ( Bothrops pubescens )

2021 ◽  
Vol 50 (3) ◽  
pp. 442-447
Author(s):  
Lina C. Bilhalva ◽  
Acácia B. Winter ◽  
Karina Oberrather ◽  
Marina R. Toso ◽  
Thaiane A. Oliveira ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Morphology ◽  
Reference Intervals ◽  
South American ◽  
Blood Cell Morphology

Evaluation of a Flow-Cytometric Osmotic Fragility Test for Hereditary Spherocytosis in Chinese Patients

2015 ◽  
Vol 135 (2) ◽  
pp. 88-93 ◽  
Author(s):  
Yi-Feng Tao ◽  
Zeng-Fu Deng ◽  
Lin Liao ◽  
Yu-Ling Qiu ◽  
Xue-Lian Deng ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Morphology ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Hereditary Spherocytosis ◽  
Osmotic Fragility ◽  
Flow Cytometric ◽  
Blood Cell Morphology

Background: Osmotic fragility testing based on flow cytometry was recently introduced for the screening of hereditary spherocytosis (HS). This study was undertaken to evaluate the clinical diagnostic value of a flow-cytometric osmotic fragility test for HS. Methods: Peripheral blood was collected from 237 subjects at the First Affiliated Hospital of Guangxi Medical University, including 56 HS patients, 86 thalassemia patients and 95 healthy controls. The samples were examined by flow-cytometric osmotic fragility test and the percentage of residual red blood cells was used to determine HS. Peripheral blood smears were performed to examine the red blood cell morphology. Results: With clinical diagnosis of HS as the gold standard and the percentage of residual red blood cells <23.6% as the diagnostic threshold in the flow-cytometric osmotic fragility test, the sensitivity of the flow-cytometric osmotic fragility test for HS was 85.71% and the specificity was 97.24%. Conclusion: The flow-cytometric osmotic fragility test combined with a red blood cell morphology test by peripheral blood smear could be a simple, practical and accurate laboratory screening method for HS.

scholarly journals Blood Cell Morphology and Plasma Biochemistry in Russian Tortoises (Agrionemys horsfieldi)

2002 ◽  
Vol 71 (2) ◽  
pp. 191-198 ◽  
Author(s):  
Z. Knotková ◽  
J. Doubek ◽  
Z. Knotek ◽  
P. Hájková
Keyword(s):  
Blood Cell ◽  
Cell Morphology ◽  
Plasma Biochemistry ◽  
Blood Cell Morphology

scholarly journals Blood cell morphology and leukocyte differential of Morelet’s crocodile (Crocodylus moreletii) (Crocodylidae)

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Adrián Gutiérrez-Cervantes ◽  
Concepción del Carmen Ahuja-Aguirre ◽  
Lorena López-deBuen ◽  
Sergio Alberto Viveros-Peredo
Keyword(s):  
Blood Cell ◽  
Cell Morphology ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Morphological Characteristics ◽  
Differential Count ◽  
Adult Males ◽  
Leukocyte Differential Count ◽  
Blood Cell Morphology ◽  
Crocodylus Moreletii

The objective of the study was to determine the morphological characteristics of peripheral blood cells (erythrocytes, leukocytes, thrombocytes) and the leukocyte differential count (heterophils, eosinophils, basophils, lymphocytes, monocytes, azurophils) of captive Morelet’s crocodiles (Crocodylus moreletii) from Veracruz, Mexico. Peripheral blood from 80 apparently healthy farmed crocodiles (39 subadults [19 females, 20 males] and 41 adults [18 females, 23 males]) was examined for morphology through stained blood smears and manual count was used for the leukocyte differential. Blood was collected during the non-breeding (n = 42) and breeding (n = 38) seasons. Blood examination indicated similar morphological characteristics of blood cells in subadult and adult individuals and in females and males in both seasons. Erythrocytes were the largest blood cells and lymphocytes the smallest. The leukocyte differential count showed that lymphocytes were the most abundant leukocytes and basophils the least numerous. The percentages of some leukocytes showed difference by season (non-breeding and breeding) in subadult and adult males (p < 0.05) and by size (subadults and adults) in males and females but only in the non-breeding season (p < 0.05). The leukocytes that showed the greatest variation were lymphocytes, heterophils and eosinophils. The knowledge of blood cell morphology and the leukocyte differential count in healthy farmed Morelet’s crocodiles will allow the accurate diagnosis of some diseases of captive and wild individuals.  

scholarly journals Iron alters red blood cell morphology

Blood ◽  
2013 ◽  
Vol 121 (1) ◽  
pp. 9-9 ◽  
Author(s):  
Etheresia Pretorius ◽  
Boguslaw Lipinski
Keyword(s):  
Blood Cell ◽  
Cell Morphology ◽  
Red Blood Cell ◽  
Blood Cell Morphology
Sign in / Sign up

Export Citation Format

Share Document