scholarly journals Soil microbial communities in the face of changing farming practices: A case study in an agricultural landscape in France

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252216
Author(s):  
Laurie Dunn ◽  
Christophe Lang ◽  
Nicolas Marilleau ◽  
Sébastien Terrat ◽  
Luc Biju-Duval ◽  
...  
Keyword(s):  
Microbial Biomass ◽  
Soil Properties ◽  
Microbial Communities ◽  
Soil Microorganisms ◽  
Soil Microbial Communities ◽  
Partial Least Square ◽  
Farming Practices ◽  
Soil Microbial ◽  
Bacterial Richness ◽  
Over Time

According to biogeography studies, the abundance and richness of soil microorganisms vary across multiple spatial scales according to soil properties and farming practices. However, soil microorganisms also exhibit poorly understood temporal variations. This study aimed at better understanding how soil microbial communities respond to changes in farming practices at a landscape scale over time. A regular grid of 269 sites was set up across a 1,200 ha farming landscape, and soil samples were characterized for their molecular microbial biomass and bacterial richness at two dates (2011 and 2016). A mapping approach highlighted that spatial microbial patterns were stable over time, while abundance and richness levels were modified. The drivers of these changes were investigated though a PLS-PM (partial least square path-modeling) approach. Soil properties were stable over time, but farming practices changed. Molecular microbial biomass was mainly driven by soil resources, whereas bacterial richness depended on both farming practices and ecological parameters. Previous-crop and management effects and a temporal dependence of the microbial community on the historical farming management were also highlighted.

scholarly journals Effects of Wildfire and Harvest Disturbances on Forest Soil Bacterial Communities

2007 ◽  
Vol 74 (1) ◽  
pp. 216-224 ◽  
Author(s):  
Nancy R. Smith ◽  
Barbara E. Kishchuk ◽  
William W. Mohn
Keyword(s):  
Microbial Biomass ◽  
Microbial Communities ◽  
Community Composition ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Microbial Biomass Carbon ◽  
Bacterial Community Composition ◽  
Soil Microbial Communities ◽  
Rrna Gene ◽  
Microbial Biomass Nitrogen ◽  
Soil Microbial

ABSTRACT Wildfires and harvesting are important disturbances to forest ecosystems, but their effects on soil microbial communities are not well characterized and have not previously been compared directly. This study was conducted at sites with similar soil, climatic, and other properties in a spruce-dominated boreal forest near Chisholm, Alberta, Canada. Soil microbial communities were assessed following four treatments: control, harvest, burn, and burn plus timber salvage (burn-salvage). Burn treatments were at sites affected by a large wildfire in May 2001, and the communities were sampled 1 year after the fire. Microbial biomass carbon decreased 18%, 74%, and 53% in the harvest, burn, and burn-salvage treatments, respectively. Microbial biomass nitrogen decreased 25% in the harvest treatment, but increased in the burn treatments, probably because of microbial assimilation of the increased amounts of available NH4 + and NO3 − due to burning. Bacterial community composition was analyzed by nonparametric ordination of molecular fingerprint data of 119 samples from both ribosomal intergenic spacer analysis (RISA) and rRNA gene denaturing gradient gel electrophoresis. On the basis of multiresponse permutation procedures, community composition was significantly different among all treatments, with the greatest differences between the two burned treatments versus the two unburned treatments. The sequencing of DNA bands from RISA fingerprints revealed distinct distributions of bacterial divisions among the treatments. Gamma- and Alphaproteobacteria were highly characteristic of the unburned treatments, while Betaproteobacteria and members of Bacillus were highly characteristic of the burned treatments. Wildfire had distinct and more pronounced effects on the soil microbial community than did harvesting.

scholarly journals Characteristics and influencing factors of soil microbial communities in different subtropical forest stands in Jinyun Mountain, Southwest China

2021 ◽  
Author(s):  
Xiaonan An ◽  
Yunqi Wang ◽  
Jialiang Zhang
Keyword(s):  
Fatty Acid ◽  
Microbial Communities ◽  
Soil Microorganisms ◽  
Phospholipid Fatty Acid ◽  
Soil Microbial Communities ◽  
Pinus Massoniana ◽  
Gram Negative Bacteria ◽  
Forest Stands ◽  
Gram Negative ◽  
Soil Microbial

Abstract BackgroundAn insight into the soil microbial functions and spatial distribution of soil resources is an important basis for evaluating and managing plant growth in subtropical forests. Soil samples were collected from five forest stands in Jinyun Mountain Natural Reserve (JMNR) in Chongqing located at the Three Gorges Reservoir area: Gordonia acuminata evergreen broad-leaved forest (GAEBF), Cunninghamia lanceolata forest (CLF), Phyllostachys pubescens forest (PPF), coniferous and broad-leaved mixed forest (CBLMF) dominated by Pinus massoniana and Gordonia acuminata (PM&GA), and the CBLMF dominated by Pinus massoniana and Symplocos setchuensis (PM&SS). Combined with phospholipid fatty acid (PLFA) analysis and Sherlock microbial identification system (MIS), the structure of soil microbial communities in different forest stands was investigated.ResultsThe results showed that the PLFAs of soil microorganisms under the forest in JMNR have a high diversity. The PLFA dominance values of the five stands were 16:0, 19:0 cyclo ω7c, 18:0, 15:0 iso and 16 :0 10-methyl. Furthermore, soil microorganisms are dominated by Gram-negative bacteria, and the PLFAs content of soil bacteria in different forest stands is higher than that of fungi PLFAs. Regarding the phospholipid fatty acid biomarkers, the two CBLMFs are the highest, followed by CLF and GAEBF and PPF is the least. Moreover, the proportion of microorganisms in the soil of different forest stands varies. Among them, MP&SS has the highest gram-negative bacteria, gram-positive bacteria, actinomycetes and fungi.ConclusionsRDA analysis shows that the main influencing factors of PLFAs in the soil of different forest stands are the content of iron oxide, aluminium oxide, organic matter and total nitrogen in the soil, which are considered to be able to reflect the soil nutrient status of JMNR effectively.

scholarly journals Ester Linked Fatty Acid (ELFA) method should be used with caution for interpretating soil microbial communities and their relationships with environmental variables in forest soils

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0251501
Author(s):  
Wenjuan Yu ◽  
Huanhuan Gao ◽  
Hongzhang Kang
Keyword(s):  
Fatty Acid ◽  
Microbial Biomass ◽  
Microbial Communities ◽  
Forest Soils ◽  
Environmental Variables ◽  
Phospholipid Fatty Acid ◽  
Soil Microbial Communities ◽  
Environmental Drivers ◽  
Soil Microbial ◽  
Forest Sites

As an alternative for phospholipid fatty acid (PLFA) analysis, a simpler ester linked fatty acid (ELFA) analysis has been developed to characterize soil microbial communities. However, few studies have compared the two methods in forest soils where the contribution of nonmicrobial sources may be larger than that of microbial sources. Moreover, it remains unclear whether the two methods yield similar relationships of microbial biomass and composition with environmental variables. Here, we compared PLFA and ELFA methods with respect to microbial biomass and composition and their relationships with environmental variables in six oriental oak (Quercus variabilis) forest sites along a 1500-km latitudinal gradient in East China. We found that both methods had a low sample-to-sample variability and successfully separated overall community composition of sites. However, total, bacterial, and fungal biomass, the fungal-to-bacterial ratio, and the gram-positive to gram-negative bacteria ratio were not significantly or strongly correlated between the two methods. The relationships of these microbial properties with environmental variables (pH, precipitation, and clay) greatly differed between the two methods. Our study indicates that despite its simplicity, the ELFA method may not be as feasible as the PLFA method for investigating microbial biomass and composition and for identifying their dominant environmental drivers, at least in forest soils.

Cattle trampling alters soil properties and changes soil microbial communities in a Swiss sub-alpine pasture

Geoderma ◽  
2012 ◽  
Vol 170 ◽  
pp. 369-377 ◽  
Author(s):  
David Hiltbrunner ◽  
Sebastian Schulze ◽  
Frank Hagedorn ◽  
Michael W.I. Schmidt ◽  
Stephan Zimmmermann
Keyword(s):  
Soil Properties ◽  
Microbial Communities ◽  
Soil Microbial Communities ◽  
Soil Microbial ◽  
Alpine Pasture

scholarly journals Flood Pulse Irrigation of Meadows Shapes Soil Chemical and Microbial Parameters More Than Mineral Fertilization

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 24
Author(s):  
Kilian G. J. Kenngott ◽  
Kai Riess ◽  
Katherine Muñoz ◽  
Gabriele E. Schaumann ◽  
Constanze Buhk ◽  
...  
Keyword(s):  
Microbial Biomass ◽  
Microbial Communities ◽  
Soil Microbial Communities ◽  
Flood Pulse ◽  
Chemical Parameters ◽  
Mineral Fertilization ◽  
Flooded Soils ◽  
Soil Microbial ◽  
Water Extractable ◽  
Soil Chemical Parameters

While mineral fertilization increases agricultural yields, it also bears the risk of contaminating non-target ecosystems and negatively affecting soil chemical parameters and microbial communities. This calls for alternative and more sustainable agricultural practices that reduce the use of fertilizers. Flood pulse irrigation could be an alternative to mineral fertilization of hay meadows, since it increases the yield with little or no application of fertilizer. However, the positive and negative implications of flood pulse irrigation on soil chemical parameters and particularly soil microbial communities are still largely unknown. In this study, we assessed shifts in soil microbial communities (SMC) as a response to changes in soil chemical parameters after flood pulse irrigation and/or fertilization of meadows. We determined soil chemical (Corg, Ntot, water extractable N, P, K, pH) and microbial (phospholipid-derived fatty acids, PLFA) parameters of 12 meadows in a 2 × 2 factorial design, comprising flood pulse irrigation and fertilization. Corg, Ntot, and water content as well as microbial biomass were higher in flood-irrigated than in non-flooded soils. Soil microbial biomass positively correlated with Corg, Ntot, and water extractable N. Gram-negative bacteria significantly increased, whereas the fungi/bacteria ratio significantly decreased in flood-irrigated soils compared to non-flooded soils. Arbuscular mycorrhizal fungi were positively correlated with soil pH. Flood pulse irrigation seemed to promote the build-up of a larger soil carbon and nitrogen pool as well as higher water content and microbial biomass. By this, it potentially mitigated negative mineral fertilization effects such as changed soil pH and reduced carbon use efficiency. We conclude that flood pulse irrigation may represent a sustainable alternative to mineral fertilization.

scholarly journals The Impacts of Different Green Manure on Soil Microbial Communities and Crop Health

2016 ◽  
Author(s):  
Hongwu Yang ◽  
Jiaojiao Niu ◽  
Jiemeng Tao ◽  
Yabing Gu ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Soil Properties ◽  
Microbial Communities ◽  
Soil Nutrients ◽  
Green Manure ◽  
Soil Microbial Communities ◽  
Disease Rate ◽  
Rrna Gene ◽  
Soil Microbial ◽  
Tobacco Disease ◽  
Crop Health

Green manure could improve soil nutrients and crop production, playing a significant role in sustainable agriculture. However, the impacts of green manure on crop health and the roles soil microbial communities play in the process haven’t been clarified clearly yet. In this study, we investigated soil microbial community composition and structure in four tobacco farmlands, which were treated with different green manure (control, ryegrass, pea and rape), using 16S rRNA gene amplicons sequencing. Results showed that green manure had significant impacts on soil properties, microbial communities and tobacco health. First, soil total C, N and Ca content increased significantly in groups treated with green manure than control. Second, soil community diversity was significantly higher in groups treated with green manure. Third, green manure especially ryegrass, decreased tobacco disease (bacterial wilt) rate dramatically, and the process might be mediated by soil microbial communities. On the one hand, several microbial populations were found to be potentially disease inducible or suppressive. For example, the abundances of Dokdonella and Rhodanobacter were positively correlated to tobacco disease rate, while Acidobacteira_Gp4 and Gp6 had negative correlations with tobacco disease. On the other hand, soil microbial communities were shaped by soil properties (e.g., pH, C and N content). In conclusion, our research showed that green manure could increase soil nutrients directly, and further improve tobacco health mediated by soil microorganisms, which may shed light on revealing interactions among soil properties, microorganisms and plants.

scholarly journals Authentic soil microbial communities in primeval forest ecosystems of Uzhanskyi National Nature Park

2017 ◽  
Vol 18 (3-4) ◽  
pp. 51-56
Author(s):  
L. Yu. Symochko ◽  
A. I. Fizer
Keyword(s):  
Microbial Communities ◽  
Functional Diversity ◽  
Soil Microorganisms ◽  
Soil Microbial Communities ◽  
Ecological Factors ◽  
Human Welfare ◽  
Soil Microbiota ◽  
High Productivity ◽  
Soil Microbial ◽  
Biomass Influence

The article presents the modern aspects of studying and evaluating the biodiversity of soil microbiocenoses in the Carpathian region. The purpose of the work was to investigate the soil microbiota of virgin ecosystems, namely the structure of microbial communities, the number of major ecological trophic groups; to analyze the successional processes occurring in the soil microbiocenosis due to the influence of endogenous and exogenous factors. Anthropogenic impact: soil compaction, sanitary felling has led to changes in the groups of soil microorganisms, reduced their numbers and functional diversity. The primeval forests as etalon ecosystems better combine above resistance and stability with high productivity of biomass. Influence of ecological factors caused changes in the community of soil organisms, varied their abundance and functional diversity. Soil microorganisms have been largely ignored by conservation efforts. However, their role in biogeochemical processes, their diversity and abundance, and their potential as repositories of valuable genetic information and metabolic products make them as important as animals and plants to the biosphere and human welfare. Study of authentic soil microbiota creates the necessary prerequisites for the conservation of microbial diversity and forming the base of the eco-microbiological monitoring.

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