New Selection Marker for Plant Transformation

2004 ◽  
pp. 385-396
Author(s):  
Barbara Leyman ◽  
Nelson Avonce ◽  
Matthew Ramon ◽  
Patrick Van Dijck ◽  
Johan M. Thevelein ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Selection Marker

scholarly journals A reporter for noninvasively monitoring gene expression and plant transformation

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yubing He ◽  
Tao Zhang ◽  
Hui Sun ◽  
Huadong Zhan ◽  
Yunde Zhao
Keyword(s):  
Gene Expression ◽  
Plant Transformation ◽  
Protein Localization ◽  
Fruit Tree ◽  
Selection Marker ◽  
Special Equipment ◽  
Chemical Treatments ◽  
Effective Selection ◽  
Monitoring Gene Expression ◽  
Large Crop

Abstract Reporters have been widely used to visualize gene expression, protein localization, and other cellular activities, but the commonly used reporters require special equipment, expensive chemicals, or invasive treatments. Here, we construct a new reporter RUBY that converts tyrosine to vividly red betalain, which is clearly visible to naked eyes without the need of using special equipment or chemical treatments. We show that RUBY can be used to noninvasively monitor gene expression in plants. Furthermore, we show that RUBY is an effective selection marker for transformation events in both rice and Arabidopsis. The new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions.

scholarly journals D-amino Acid Oxidase (DAO) Gene as a Novel Selection Marker for Plant Transformation

2007 ◽  
Vol 34 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Sun-Hyung Lim ◽  
Hee-Jong Woo ◽  
Si-Myung Lee ◽  
Yong-Moon Jin ◽  
Hyun-Suk Cho
Keyword(s):  
Amino Acid ◽  
Plant Transformation ◽  
Selection Marker ◽  
Acid Oxidase ◽  
Amino Acid Oxidase

Gateway Binary Vectors with the Bialaphos Resistance Gene,bar, as a Selection Marker for Plant Transformation

2010 ◽  
Vol 74 (6) ◽  
pp. 1315-1319 ◽  
Author(s):  
Shinya NAKAMURA ◽  
Shoji MANO ◽  
Yuji TANAKA ◽  
Masato OHNISHI ◽  
Chihiro NAKAMORI ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Plant Transformation ◽  
Selection Marker ◽  
Binary Vectors

Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation

Planta ◽  
2006 ◽  
Vol 225 (4) ◽  
pp. 897-906 ◽  
Author(s):  
Paoyuan Hsiao ◽  
Sanjaya ◽  
Ruey-Chih Su ◽  
Jaime A. Teixeira da Silva ◽  
Ming-Tsair Chan
Keyword(s):  
Plant Transformation ◽  
Selection Marker ◽  
Tryptophan Synthase ◽  
Antibiotic Selection

scholarly journals Anthocyanin production as a potential visual selection marker during plant transformation

2011 ◽  
Vol 20 (6) ◽  
pp. 1253-1264 ◽  
Author(s):  
A. J. Kortstee ◽  
S. A. Khan ◽  
C. Helderman ◽  
L. M. Trindade ◽  
Y. Wu ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Visual Selection ◽  
Selection Marker ◽  
Anthocyanin Production

scholarly journals Trehalose-6-phosphate synthase as an intrinsic selection marker for plant transformation

2006 ◽  
Vol 121 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Barbara Leyman ◽  
Nelson Avonce ◽  
Matthew Ramon ◽  
Patrick Van Dijck ◽  
Gabriel Iturriaga ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Selection Marker ◽  
Phosphate Synthase

Plant transformation by microinjection techniques

1990 ◽  
Vol 79 (1) ◽  
pp. 213-217 ◽  
Author(s):  
Gunther Neuhaus ◽  
German Spangenberg
Keyword(s):  
Plant Transformation ◽  
Microinjection Techniques

scholarly journals Identification of the Propionicin F Bacteriocin Immunity Gene (pcfI) and Development of a Food-Grade Cloning System for Propionibacterium freudenreichii

2007 ◽  
Vol 73 (23) ◽  
pp. 7542-7547 ◽  
Author(s):  
Dag Anders Brede ◽  
Sheba Lothe ◽  
Zhian Salehian ◽  
Therese Faye ◽  
Ingolf F. Nes
Keyword(s):  
Selection Marker ◽  
Multiple Cloning Site ◽  
Propionibacterium Freudenreichii ◽  
Expression Plasmid ◽  
Physiochemical Properties ◽  
Food Grade ◽  
Cloning System ◽  
Immunity Gene ◽  
Membrane Bound ◽  
High Level

ABSTRACT This report describes the first functional analysis of a bacteriocin immunity gene from Propionibacterium freudenreichii and its use as a selection marker for food-grade cloning. Cloning of the pcfI gene (previously orf5 [located as part of the pcfABC propionicin F operon]) rendered the sensitive host 1,000-fold more tolerant to the propionicin F bacteriocin. The physiochemical properties of the 127-residue large PcfI protein resemble those of membrane-bound immunity proteins from bacteriocin systems found in lactic acid bacteria. The high level of immunity conferred by pcfI allowed its use as a selection marker for plasmid transformation in P. freudenreichii. Electroporation of P. freudenreichii IFO12426 by use of the pcfI expression plasmid pSL102 and propionicin F selection (200 bacteriocin units/ml) yielded 107 transformants/μg DNA. The 2.7-kb P. freudenreichii food-grade cloning vector pSL104 consists of the pLME108 replicon, a multiple cloning site, and pcfI expressed from the constitutive PpampS promoter for selection. The pSL104 vector efficiently facilitated cloning of the propionicin T1 bacteriocin in P. freudenreichii. High-level propionicin T1 production (640 BU/ml) was obtained with the IFO12426 strain, and the food-grade propionicin T1 expression plasmid pSL106 was maintained by ∼91% of the cells over 25 generations in the absence of selection. To the best of our knowledge this is the first report of an efficient cloning system that facilitates the generation of food-grade recombinant P. freudenreichii strains.

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