scholarly journals A reporter for noninvasively monitoring gene expression and plant transformation

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yubing He ◽  
Tao Zhang ◽  
Hui Sun ◽  
Huadong Zhan ◽  
Yunde Zhao
Keyword(s):  
Gene Expression ◽  
Plant Transformation ◽  
Protein Localization ◽  
Fruit Tree ◽  
Selection Marker ◽  
Special Equipment ◽  
Chemical Treatments ◽  
Effective Selection ◽  
Monitoring Gene Expression ◽  
Large Crop

Abstract Reporters have been widely used to visualize gene expression, protein localization, and other cellular activities, but the commonly used reporters require special equipment, expensive chemicals, or invasive treatments. Here, we construct a new reporter RUBY that converts tyrosine to vividly red betalain, which is clearly visible to naked eyes without the need of using special equipment or chemical treatments. We show that RUBY can be used to noninvasively monitor gene expression in plants. Furthermore, we show that RUBY is an effective selection marker for transformation events in both rice and Arabidopsis. The new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions.

scholarly journals Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Wiruntita Chankeaw ◽  
Sandra Lignier ◽  
Christophe Richard ◽  
Theodoros Ntallaris ◽  
Mariam Raliou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Localization ◽  
Expression Profiles ◽  
Cell Types ◽  
Molecular Signature ◽  
Receptor Protein ◽  
Specific Gene ◽  
Specific Cell ◽  
Biological Processes ◽  
Endometrial Cells

Abstract Background A number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of pregnancy. However, to date, these studies have been performed on the whole endometrium which is a complex tissue. Consequently, the knowledge of cell-specific gene expression, when analysis performed with whole endometrium, is still weak and obviously limits the relevance of the results of gene expression studies. Thus, the aim of this study was to characterize specific transcriptome of the three main cell-types of the bovine endometrium at day-15 of the estrus cycle. Results In the RNA-Seq analysis, the number of expressed genes detected over 10 transcripts per million was 6622, 7814 and 8242 for LE, GE and ST respectively. ST expressed exclusively 1236 genes while only 551 transcripts were specific to the GE and 330 specific to LE. For ST, over-represented biological processes included many regulation processes and response to stimulus, cell communication and cell adhesion, extracellular matrix organization as well as developmental process. For GE, cilium organization, cilium movement, protein localization to cilium and microtubule-based process were the only four main biological processes enriched. For LE, over-represented biological processes were enzyme linked receptor protein signaling pathway, cell-substrate adhesion and circulatory system process. Conclusion The data show that each endometrial cell-type has a distinct molecular signature and provide a significantly improved overview on the biological process supported by specific cell-types. The most interesting result is that stromal cells express more genes than the two epithelial types and are associated with a greater number of pathways and ontology terms.

Monitoring gene expression along pear fruit development, ripening and senescence using cDNA microarrays

Plant Science ◽  
2004 ◽  
Vol 167 (3) ◽  
pp. 457-469 ◽  
Author(s):  
Sandra Fonseca ◽  
László Hackler ◽  
Ágnes Zvara ◽  
Sı́lvia Ferreira ◽  
Aladje Baldé ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruit Development ◽  
Cdna Microarrays ◽  
Pear Fruit ◽  
Monitoring Gene Expression

The Human Carotid Body

2010 ◽  
Vol 113 (6) ◽  
pp. 1270-1279 ◽  
Author(s):  
Malin Jonsson Fagerlund ◽  
Jessica Kåhlin ◽  
Anette Ebberyd ◽  
Gunnar Schulte ◽  
Souren Mkrtchian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathways ◽  
Nicotinic Acetylcholine Receptors ◽  
Carotid Body ◽  
Protein Localization ◽  
Oxygen Sensing ◽  
Radical Neck Dissection ◽  
K Channel ◽  
General Anesthetics ◽  
Anesthetic Drugs

Background Hypoxia is a common cause of adverse events in the postoperative period, where respiratory depression due to residual effects of drugs used in anesthesia is an important underlying factor. General anesthetics and neuromuscular blocking agents reduce the human ventilatory response to hypoxia. Although the carotid body (CB) is the major oxygen sensor in humans, critical oxygen sensing and signaling pathways have been investigated only in animals so far. Thus, the aim of this study was to characterize the expression of key genes and localization of their products involved in the human oxygen sensing and signaling pathways with a focus on receptor systems and ion channels of relevance in anesthesia. Methods Six CBs were removed unilaterally from patients undergoing radical neck dissection. The gene expression and cell-specific protein localization in the CBs were investigated with DNA microarrays, real-time polymerase chain reaction, and immunohistochemistry. Results We found gene expression of the oxygen-sensing pathway, heme oxygenase 2, and the K channels TASK (TWIK-related acid sensitive K channel)-1 and BK (large-conductance potassium channel). In addition, we show the expression of critical receptor subunits such as γ-aminobutyric acid A (α2, β3, and γ2), nicotinic acetylcholine receptors (α3, α7, and β2), purinoceptors (A2A and P2X2), and the dopamine D2 receptor. Conclusions In unique samples of the human CB, we here demonstrate presence of critical proteins in the oxygen-sensing and signaling cascade. Our findings demonstrate similarities to, but also important differences from, established animal models. In addition, our work establishes an essential platform for studying the interaction between anesthetic drugs and human CB chemoreception.

New Selection Marker for Plant Transformation

2004 ◽  
pp. 385-396
Author(s):  
Barbara Leyman ◽  
Nelson Avonce ◽  
Matthew Ramon ◽  
Patrick Van Dijck ◽  
Johan M. Thevelein ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Selection Marker

Monitoring Gene Expression in Plant Tissues

2006 ◽  
pp. 31-46
Author(s):  
John J. Finer ◽  
Summer L. Beck ◽  
Marco T. Buenrostro-Nava ◽  
Yu-Tseh Chi ◽  
Peter P. Ling
Keyword(s):  
Gene Expression ◽  
Plant Tissues ◽  
Monitoring Gene Expression

scholarly journals Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women

2019 ◽  
Vol 21 (1) ◽  
pp. 295
Author(s):  
Rebeca González-Fernández ◽  
Rita Martín-Ramírez ◽  
Deborah Rotoli ◽  
Jairo Hernández ◽  
Frederick Naftolin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Localization ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cellular Protein ◽  
Control Group ◽  
Poor Responders ◽  
Male Factor ◽  
Expression Levels ◽  
Genes Expression

Sirtuins are a family of deacetylases that modify structural proteins, metabolic enzymes, and histones to change cellular protein localization and function. In mammals, there are seven sirtuins involved in processes like oxidative stress or metabolic homeostasis associated with aging, degeneration or cancer. We studied gene expression of sirtuins by qRT-PCR in human mural granulosa-lutein cells (hGL) from IVF patients in different infertility diagnostic groups and in oocyte donors (OD; control group). Study 1: sirtuins genes’ expression levels and correlations with age and IVF parameters in women with no ovarian factor. We found significantly higher expression levels of SIRT1, SIRT2 and SIRT5 in patients ≥40 years old than in OD and in women between 27 and 39 years old with tubal or male factor, and no ovarian factor (NOF). Only SIRT2, SIRT5 and SIRT7 expression correlated with age. Study 2: sirtuin genes’ expression in women poor responders (PR), endometriosis (EM) and polycystic ovarian syndrome. Compared to NOF controls, we found higher SIRT2 gene expression in all diagnostic groups while SIRT3, SIRT5, SIRT6 and SIRT7 expression were higher only in PR. Related to clinical parameters SIRT1, SIRT6 and SIRT7 correlate positively with FSH and LH doses administered in EM patients. The number of mature oocytes retrieved in PR is positively correlated with the expression levels of SIRT3, SIRT4 and SIRT5. These data suggest that cellular physiopathology in PR’s follicle may be associated with cumulative DNA damage, indicating that further studies are necessary.

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