Extraction and Characterization of Vaccine Antigens from Water-in-Oil Adjuvant Formulations

2005 ◽  
pp. 293-300 ◽  
Author(s):  
Aaron P. Miles ◽  
Allan Saul
Keyword(s):  
Vaccine Antigens

scholarly journals Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection

Vaccines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 153 ◽  
Author(s):  
Tianmou Zhu ◽  
Ryan McClure ◽  
Odile B. Harrison ◽  
Caroline Genco ◽  
Paola Massari
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Recombinant Expression ◽  
Selection Strategy ◽  
Antibiotic Resistant ◽  
Sexually Transmitted ◽  
Vaccine Antigens ◽  
Mucosal Infection ◽  
Associated Proteins

There is an increasingly severe trend of antibiotic-resistant Neisseria gonorrhoeae strains worldwide and new therapeutic strategies are needed against this sexually-transmitted pathogen. Despite the urgency, progress towards a gonococcal vaccine has been slowed by a scarcity of suitable antigens, lack of correlates of protection in humans and limited animal models of infection. N. gonorrhoeae gene expression levels in the natural human host does not reflect expression in vitro, further complicating in vitro-basedvaccine analysis platforms. We designed a novel candidate antigen selection strategy (CASS), based on a reverse vaccinology-like approach coupled with bioinformatics. We utilized the CASS to mine gonococcal proteins expressed during human mucosal infection, reported in our previous studies, and focused on a large pool of hypothetical proteins as an untapped source of potential new antigens. Via two discovery and analysis phases (DAP), we identified 36 targets predicted to be immunogenic, membrane-associated proteins conserved in N. gonorrhoeae and suitable for recombinant expression. Six initial candidates were produced and used to immunize mice. Characterization of the immune responses indicated cross-reactive antibodies and serum bactericidal activity against different N. gonorrhoeae strains. These results support the CASS as a tool for the discovery of new vaccine candidates.

scholarly journals Characterization of human enterovirus71 virus-like particles used for vaccine antigens

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181182 ◽  
Author(s):  
Dandan Zhao ◽  
Bo Sun ◽  
Shiyang Sun ◽  
Bin Fu ◽  
Chuntian Liu ◽  
...  
Keyword(s):  
Virus Like Particles ◽  
Vaccine Antigens

scholarly journals Serological characterization of surface-exposed proteins of Coxiella burnetii

Microbiology ◽  
2014 ◽  
Vol 160 (12) ◽  
pp. 2718-2731 ◽  
Author(s):  
Jun Jiao ◽  
Xiaolu Xiong ◽  
Yong Qi ◽  
Wenping Gong ◽  
Changsong Duan ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Bioinformatics Analysis ◽  
Q Fever ◽  
Obligate Intracellular ◽  
Rickettsia Rickettsii ◽  
Serological Characterization ◽  
Vaccine Antigens ◽  
Mycoplasma Pneumonia ◽  
Dimensional Electrophoresis

The obligate intracellular Gram-negative bacterium Coxiella burnetii causes Q fever, a worldwide zoonosis. Here we labelled Cox . burnetii with biotin and used biotin-streptavidin affinity chromatography to isolate surface-exposed proteins (SEPs). Using two-dimensional electrophoresis combined with mass spectrometry, we identified 37 proteins through bioinformatics analysis. Thirty SEPs expressed in Escherichia coli (recombinant SEPs, rSEPs) were used to generate microarrays, which were probed with sera from mice experimentally infected with Cox. burnetii or sera from Q fever patients. Thirteen rSEPs were recognized as seroreactive, and the majority reacted with at least 50 % of the sera from mice infected with Cox. burnetii but not with sera from mice infected with Rickettsia rickettsii, R. heilongjiangensis, or R. typhi. Further, 13 proteins that reacted with sera from patients with Q fever did not react with sera from patients with brucellosis or mycoplasma pneumonia. Our results suggest that these seroreactive SEPs have potential as serodiagnostic antigens or as subunit vaccine antigens against Q fever.

scholarly journals Characterization of Serological Responses to Pertussis

2006 ◽  
Vol 13 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Mineo Watanabe ◽  
Beverly Connelly ◽  
Alison A. Weiss
Keyword(s):  
Adenylate Cyclase ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vaccine Antigens ◽  
History Of ◽  
Antibody Titers ◽  
Iga Antibody ◽  
Conventional Vaccine ◽  
Culture Positive

ABSTRACT We have compared the use of five nonvaccine antigens to the use of conventional vaccine antigens, pertussis toxin (PT), and filamentous hemagglutinin (FHA) for the serological diagnosis of pertussis by enzyme-linked immunosorbent assay (ELISA). The nonvaccine antigens included the catalytic region of adenylate cyclase toxin (CatACT), the C-terminal region of FHA (C-FHA), lipooligosaccharide (LOS), the peptidoglycan-associated lipoprotein (PAL), and the BrkA protein. The serological responses of individuals with culture-confirmed pertussis were compared to those of adults with no recent history of a coughing disease. An immunoglobulin G (IgG) ELISA for PT was the most sensitive (92.2%) test for the serodiagnosis of pertussis. Of the nonvaccine antigens, ELISA for IgG responses to CatACT (sensitivity, 62.8%), C-FHA (sensitivity, 39.2%), and LOS IgA (sensitivity, 29.4%) were less sensitive but could also distinguish culture-positive individuals from control individuals. The use of a combination of multiple ELISA targets improved the sensitivity of the assay for serological diagnosis. Elevated IgG and IgA antibody titers persisted for more than a year in the individuals with culture-confirmed pertussis.

scholarly journals Characterization of proteins Msp22 and Msp75 as vaccine antigens of Moraxella catarrhalis

Vaccine ◽  
2009 ◽  
Vol 27 (50) ◽  
pp. 7065-7072 ◽  
Author(s):  
Elizabeth A. Ruckdeschel ◽  
Aimee L. Brauer ◽  
Antoinette Johnson ◽  
Timothy F. Murphy
Keyword(s):  
Moraxella Catarrhalis ◽  
Vaccine Antigens

Physicochemical and functional characterization of vaccine antigens and adjuvants

2014 ◽  
Vol 13 (5) ◽  
pp. 671-685 ◽  
Author(s):  
Antu K Dey ◽  
Padma Malyala ◽  
Manmohan Singh
Keyword(s):  
Functional Characterization ◽  
Vaccine Antigens

scholarly journals Molecular and structural characterization of the L1 virus-like particles that are used as vaccine antigens inCervarix™, the AS04-adjuvanted HPV-16 and -18 cervical cancer vaccine

2010 ◽  
Vol 6 (5) ◽  
pp. 407-419 ◽  
Author(s):  
Michel Deschuyteneer ◽  
Abdelatif Elouahabi ◽  
Dominique Plainchamp ◽  
Michel Plisnier ◽  
Dominique Soete ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Vaccine ◽  
Structural Characterization ◽  
Hpv 16 ◽  
Virus Like Particles ◽  
Vaccine Antigens ◽  
Cervical Cancer Vaccine

Meningococcal Serogroup B Disease in Vaccinated Children

2019 ◽  
Vol 9 (4) ◽  
pp. 454-459
Author(s):  
Aleix Soler-Garcia ◽  
Mariona Fernández de Sevilla ◽  
Raquel Abad ◽  
Cristina Esteva ◽  
Laia Alsina ◽  
...  
Keyword(s):  
Unknown Origin ◽  
Vaccine Antigen ◽  
Heparin Binding ◽  
Vaccine Antigens ◽  
Antigen Peptide ◽  
Vaccination History ◽  
Diagnostic Work ◽  
High Level ◽  
Work Up

Abstract Background Neisseria meningitidis serogroup B (MenB) is the most frequent cause of invasive meningococcal disease (IMD) in Spain. The multicomponent vaccine against MenB (4CMenB) was approved in Spain in January 2014. Methods We present 4 cases of children who developed MenB-associated IMD despite previous vaccination with 4CMenB. Extensive immunologic diagnostic work-up was performed in order to rule out any immunodeficiency. Also, molecular characterization of the MenB strain was conducted to determine whether bacterial antigens matched vaccine antigens. Results Among the 4 patients (2 girls), 2 had previous risk factors for IMD (recurrent bacterial meningitis of unknown origin and treatment with eculizumab). All patients developed meningitis, but only 2 developed septic shock; they were all cured without sequelae. No other primary or secondary immunodeficiencies were detected. MenB sequence type 213 was identified in 3 cases. With the exception of neisserial heparin-binding antigen peptide 465 present in 1 isolate, the rest of the isolated strains harbored vaccine antigen variants that did not match antigen variants included in the vaccine. Conclusions We present 4 children who developed MenB-associated IMD despite previous vaccination with 4CMenB. In 2 cases, the antibodies induced by 4CMenB likely were not effective against the isolated strains. A high level of suspicion for IMD seems advisable regardless of the patient’s vaccination history.

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