Different aspects of the Activin/1 Smad pathway involvement in 2 zebrafish development

The International Journal of Developmental Biology ◽

10.1387/ijdb.210073se ◽

2021 ◽

Author(s):

Shaghayegh Hasanpour ◽

Soheil Eagderi ◽

Hadi Poorbagher ◽

Mohammad Hasanpour

Keyword(s):

Broad Spectrum ◽

Signal Intensity ◽

Activin A ◽

Smad Pathway ◽

Zebrafish Development ◽

Pluripotent State ◽

Maternal To Zygotic Transition

To investigate the role of maternal Activin-like factors in the preservation of stemness and mesendoderm induction, their effects were promoted and inhibited using synthetic human Activin A or SB-505124 treatments, respectively, before the maternal to zygotic transition (MZT). To study the role of zygotic Activin-like factors, SB-505124 treatment was also used after the MZT. Promoting the signaling intensity of maternal Activin-like factors led to premature differentiation, loss of stemness, and no mesendoderm malformation, while its alleviation delayed the differentiation and caused various malformations. Inhibition of the zygotic Activin-like factors was associated with suppressing the notail transcription, differentiation retardation at the oblong stage, and a broad spectrum of anomalies in a dosedependent manner. Together, promoting the signal intensity of maternal Activin-like factors drove development along with mesendodermal differentiation, while suppression of the maternal or zygotic ones maintained the pluripotent state and delayed the differentiation.

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THE ROLE OF PERIPLASMIC CHAPERONS IN FORMATION OF REDUCED SENSITIVITY OF THE GONOCOCCUS TO BROAD-SPECTRUM ANTIBIOTICS

Molecular Diagnostics and Biosafety – 2020. Russian national scientific and practical conference with international participation (October, 6–8, 2020): Conference Proceedings ◽

10.36233/978-5-9900432-9-9-229 ◽

2020 ◽

Author(s):

I.N. Bodoev ◽

◽

M.V. Malahova ◽

O.V. Pobeguts ◽

I.O. Butenko ◽

...

Keyword(s):

Broad Spectrum ◽

Broad Spectrum Antibiotics

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Understanding the Role of Chlorine and Ozone to Control Postharvest Diseases in Fruit and Vegetables: A Review

Current Nutrition & Food Science ◽

10.2174/1573401315666190212161209 ◽

2020 ◽

Vol 16 (4) ◽

pp. 455-461

Author(s):

Gabriela M. Baia ◽

Otniel Freitas-Silva ◽

Murillo F. Junior

Keyword(s):

Chlorine Dioxide ◽

Broad Spectrum ◽

Fruits And Vegetables ◽

Low Cost ◽

Residual Effects ◽

Pathogenic Microorganisms ◽

Postharvest Diseases ◽

Post Harvest ◽

By Products

Fruits and vegetables are foods that come into contact with various types of microorganisms from planting to their consumption. A lack or poor sanitation of these products after harvest can cause high losses due to deterioration and/ or pathogenic microorganisms. There are practically no post-harvest fungicides or bactericides with a broad spectrum of action that have no toxic residual effects and are safe. However, to minimize such problems, the use of sanitizers is an efficient device against these microorganisms. Chlorine is the most prevalent sanitizing agent because of its broad spectrum, low cost and well-established practices. However, the inevitable formation of disinfection by-products, such as trihalomethanes (THMs) and haloacetic acids (HAAs), is considered one of the main threats to food safety. Alternative sanitizers, such as chlorine dioxide (ClO2) and ozone, are becoming popular as a substitute for traditional post-harvest treatments. Thus, this review addresses the use of chlorine, chlorine dioxide and ozone emphasizing aspects, such as usage, safe application, spectrum of action and legislation. In order to ensure the quality and safety of final products, the adoption of well-prepared sanitation and sanitation programs for post-harvest fruits and vegetables is essential.

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In vitro evaluation of broad-spectrum β-lactams in the Philippines medical centers: role of fourth-generation cephalosporins

Diagnostic Microbiology and Infectious Disease ◽

10.1016/s0732-8893(99)00100-5 ◽

1999 ◽

Vol 35 (4) ◽

pp. 291-297 ◽

Cited By ~ 8

Author(s):

David M. Johnson ◽

Douglas J. Biedenbach ◽

Ronald N. Jones

Keyword(s):

Broad Spectrum ◽

The Philippines ◽

Fourth Generation ◽

In Vitro Evaluation ◽

Medical Centers

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Degradation of a broad spectrum of trace organic contaminants by an enzymatic membrane reactor: Complementary role of membrane retention and enzymatic degradation

International Biodeterioration & Biodegradation ◽

10.1016/j.ibiod.2014.12.004 ◽

2015 ◽

Vol 99 ◽

pp. 115-122 ◽

Cited By ~ 38

Author(s):

Luong N. Nguyen ◽

Faisal I. Hai ◽

William E. Price ◽

Jinguo Kang ◽

Frederic D.L. Leusch ◽

...

Keyword(s):

Broad Spectrum ◽

Organic Contaminants ◽

Membrane Reactor ◽

Enzymatic Degradation ◽

Trace Organic Contaminants ◽

Complementary Role ◽

Trace Organic ◽

Enzymatic Membrane Reactor ◽

Enzymatic Membrane

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The Role of Activin A and B and the Benefit of Follistatin Treatment in Renal Ischemia-Reperfusion Injury in Mice

Transplantation Direct ◽

10.1097/txd.0000000000000601 ◽

2016 ◽

Vol 2 (7) ◽

pp. e87 ◽

Cited By ~ 9

Author(s):

Doreen Y.P. Fang ◽

Bo Lu ◽

Susan Hayward ◽

David M. de Kretser ◽

Peter J. Cowan ◽

...

Keyword(s):

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Activin A ◽

Renal Ischemia ◽

Renal Ischemia Reperfusion Injury

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Role of p38 mitogen‐activated protein kinase signalling in virus replication and potential for developing broad spectrum antiviral drugs

Reviews in Medical Virology ◽

10.1002/rmv.2217 ◽

2021 ◽

Author(s):

Yogesh Chander ◽

Ram Kumar ◽

Nitin Khandelwal ◽

Namita Singh ◽

Brij Nandan Shringi ◽

...

Keyword(s):

Protein Kinase ◽

Virus Replication ◽

Broad Spectrum ◽

Antiviral Drugs ◽

Mitogen Activated Protein Kinase ◽

Mitogen Activated Protein ◽

Protein Kinase Signalling

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Abstract 172: The Role of Activin A in Maternal Diabetes-Induced Cardiac Malformations

Circulation Research ◽

10.1161/res.111.suppl_1.a172 ◽

2012 ◽

Vol 111 (suppl_1) ◽

Author(s):

Zhiyong Zhao

Keyword(s):

High Glucose ◽

Heart Defects ◽

Maternal Diabetes ◽

Activin A ◽

Brdu Incorporation ◽

Control Group ◽

Endocardial Cushions ◽

Diabetic Embryopathy ◽

Cardiac Malformations

Diabetes mellitus in pregnancy causes heart defects, especially atrioventricular septal defects (AVSDs), in infants. Manifestation of AVSDs results from dysmorphogenesis of the endocardial cushions in the embryo, which is regulated by members of the transforming growth factor (TGF) β family. Among the TGFβs, we have previously observed that the inhibin βA gene, which encodes a protein to form homodimers as activin A, is markedly downregulated by maternal diabetes. To further determine the involvement of inhibin βA and its signaling in diabetic embryopathy, the levels of inhibin βA protein and activation of its downstream transcription factors, Smad2 and Smad3, were examined using immunohistochemical, proximity ligation, and immunoblot assays, and shown to be decreased in the embryonic hearts of diabetic mice induced via intravenous injection of streptozotocin. To investigate the role of activin A in hyperglycemia-induced cardiac malformations, mouse embryos at embryonic day 9.5 (E9.5) cultured in high glucose (22 mM) were treated with activin A (50 ng/ml) for 24 hours. The treatment rescued the development of the endocardial cushions and cell proliferation (BrdU-incorporation assay) in the myocardium, similar to those in normal glucose control (8.3 mM). The role of activin A in endocardial cell migration, an important process for cellularization of the cardiac jelly, was investigated by treating E10.5 endocardial cushion explants cultured in high glucose with activin A (50 ng/ml) in a collagen matrix-based assay system. The treatment significantly increased the number of migrating cells, compared with that in high glucose-treated group, to the level in the control group. These effects were associated with restoration of the activation of Smad2/3. The results demonstrate that the activin-Smad2/3 signaling system plays an important role in cardiac malformation in diabetic embryopathy.

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