scholarly journals Overexpression of Sugarcane Sucrose Transporter 1 (SoSUT1) Gene Increases Rice Yield

2020 ◽  
Vol 24 (1) ◽  
pp. 18
Author(s):  
Cesha Ananda Putri ◽  
Bambang Soegiharto ◽  
Parawita Dewanti

The growth and development of plants are determined by photosynthesis, which ultimately results in sucrose. Sucrose is synthesized in the source then translocated to all parts of the plant (sink). The translocation process of sucrose from source to sink is controlled by sucrose protein called sucrose transporter. SoSUT1 is a gene that encodes a sucrose transporter 1 (SUT1) protein in sugarcane. Rice transformation with the SoSUT1 causes overexpression SUT, which is expected to increase the translocation of sucrose into the seed of rice plants. This research was conducted by introducing SoSUT1 in rice plants Inpari 14 SS. Transformation using Agrobacterium tumefaciens vector in apical bud explant Indica rice cv. Inpari 14 SS results in 26 events positive rice contains genes SoSUT1. This study aims to elucidate the inheritance of transgene in the next generation and to characterize its effect on the morphology and the yields of a subsequent generation. The study is conducted by planting the seeds of T1 and T2 plants in media containing Hygromycin and using PCR analysis for further analysis. As the results, from 26 events on the T1 plant, only 3 events of T3 plants were confirmed on the T3 plant. The overexpression of the SoSUT1 gene could increase the number of tillers, the number of productive tillers, panicle length, and panicle exit length, also increasing the number of spherical grains, reducing the number of empty grains and increasing the weight of 1000 grains.

2019 ◽  
Vol 90 (1) ◽  
pp. 89-100 ◽  
Author(s):  
Xin Xu ◽  
Mengchen Zhang ◽  
Qun Xu ◽  
Yue Feng ◽  
Xiaoping Yuan ◽  
...  

Abstract Panicle structure and grain shape are important components of rice architecture that directly contribute to rice yield and are regulated by quantitative trait loci (QTLs). In this study, a doubled haploid (DH) population derived from a cross between japonica “Maybelle” and indica “Baiyeqiu” was used to determine genetic effects on panicle structure and grain shape. All detected traits exhibited a continuous, transgressive distribution in the DH population. QTL analysis showed that a total of 24 QTLs related to panicle structure and grain shape were detected on chromosomes 1, 2, 3, 4, 5, 6, 7, 9, 10, and 12, and three epistatic interaction QTLs were detected. Some genes related to panicle structure and grain shape were predicted in the major QTLs, and variations existed between the parents in all genes. Only OsIDS1, GS5, and SRS3 had nonsynonymous mutations that led to protein changes. Quantitative real-time PCR analysis showed that the expression levels of GS5 and OsFOR1 significantly differed between the two parents. In addition, genetic diversity analysis showed that the H3-35–H3-37, H6-18–H6-19, and H7-12–H7-14 intervals might be selected in the breeding program.


2015 ◽  
Vol 172 ◽  
pp. 1-10 ◽  
Author(s):  
Lijie Zhou ◽  
Shanshan Liang ◽  
Kimberley Ponce ◽  
Severino Marundon ◽  
Guoyou Ye ◽  
...  

2021 ◽  
Vol 232 ◽  
pp. 03022
Author(s):  
Mirawanty Amin ◽  
Rahadian Adi Prasetyo ◽  
Chandra Padang

Silicon (Si) is one of the important things for rice growth. The importance of Si relates to increased rice yield, increased resistance to disease and resistance to falling. The rice is an accumulator plant of Si which requires Si for its growth. However, the Si in soil is continually decreased therefore the additional Si is needed intothe soil. Trass is one of the natural materials containing Ca and Si, therefore it can be used as a Si fertilizer. This study aims to determine levels of soil Si, straw, husk, and growth in rice. This research consisted of three types of soil, Oxisol, Inceptisol and Vertisol as the main plot and 7 doses of burnt fuel namely 0 (D0); 1.25 (D1); 2.5 (D2); 3.75 (D3); 5 (D4); 7.5 (D5); 10 (D6) g kg-1 of soil as subplots. The treatment which is using in this research is a combination of the two factors and repeated 3 times 63 unit experiments were obtained. The results showed the Trass significant concerning to Si in the husk and Si of the soil. Vertisol gives the highest number of panicles of 27 and Si in the straw of 12.75 mg kg-1.


2021 ◽  
Author(s):  
Si-Wen Liu ◽  
Li-Ding Zhang ◽  
Jia-Yu Gao ◽  
Tian-Tian Dong ◽  
Tong Zhang ◽  
...  

Abstract Source-to-sink transport of sucrose mediated by sucrose transporters (SUCs) is one of the major determinants of plant growth. However, the role of AtSUC4, the only member of Group IV sucrose transporter in Arabidopsis, was undervalued in sink organ during seedling period. In our study, the primary root length of the atsuc4 mutants was significantly longer than that of the wild-type (WT) under exogenous 4% and 6% sucrose treatment. But this phenotype could not be imitated by external application of glucose or mannitol. It means that the atsuc4 mutants were insensitive to high sucrose stress compared with WT. Meanwhile, HPLC-MS/MS results showed that the root of atsuc4 mutants accumulated less sucrose and ABA and more IAA content compared with WT on 4% and 6% sucrose supplementation. Transcriptome analysis showed that many key genes involved in IAA and ABA signals were respective stimulated and repressed in the atsuc4 mutants, respectively. Taken together, we concluded that the deficiency of AtSUC4, not only reducing the transported and uptaked of sucrose, but also as a signal, may be collaborated with IAA and ABA to regulate root growth under high sucrose stress. This study confirmed the new function of AtSUC4, and provided an promising candidate gene for improving tolerance to high sucrose stress.


2020 ◽  
Vol 50 (2) ◽  
pp. 32-38
Author(s):  
S. V. Bezmutko ◽  
V. N. Lelyavskaya

The results of research into rice sown for grain (2017–2018) are presented. The effectiveness of Concord fungicide (active ingredients tebuconazole, 125 g/l; triadimefon, 100 g/l) against rice Pyricularia was studied. The experiment was conducted in the conditions of the greenhouse experiment in the Primorsky Territory. Artificial inoculation of plants was carried out in the phase of 4–5 leaves with a suspension of conidia of the fungus Pyricularia oryzae Broomeet Cavara (concentration of 100 thousand in 1 ml). The test results were compared with the action of the preparation Favorit (active ingredients tebuconazole, 125 g/l; triadimefon, 100 g/l). Concord fungicide was used once on the next day after inoculation at a normal rate of 0.75; 1.0 and 1.25 l/ha. Under the influence of the preparation, the damage to rice plants decreased by 57.5% (0.75 l/ ha); 70.0 (1.0) and 69.5% (1.25 l/ ha) compared to the control. The use of fungicide contributed to an increase in plant growth by 7.6– 13.3 cm, and panicle length by 1.4–2.9 cm, and had a positive effect on the number of grains and the weight of grains in an ear. With the action of Concord, the mass of 1000 grains increased by 1.38 (0.75 l/ha); 2.14 (1.0) and 3.03 g (1.25 l/ha) compared to the control. The positive effect of fungicidal treatments of rice crops led to obtaining an additional grain yield. Concord does not show a phytotoxic effect and does not have a retardant effect on rice plants. The maximum indicators of biological efficiency were obtained with the use of the preparation at a flow rate of 1.0 and 1.25 l/ha. In agro-climatic conditions of the south of the Far East, Concord fungicide proved to be effective for protecting rice vegetating plants from Pyricularia.


2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Hastin Wulan Sekarweni ◽  
Yulia Pujiastuti ◽  
Siti Herlinda

Rice-field rat is a main pest of rice. Yield reduction caused by rat is quite high, because rats attack rice plant at all stages. One of the efforts applied to control rats is by using Trap Barrier System combined with cage trap. The objective of this research was to identify species of rat attacking rice plants and investigate the presence of  rat by their footprints. The experiment was conducted in Jalur 6  Village Sumber Mulya, Sub-district  Muara Telang,  District Banyuasin, South  Sumatra. One hectare of farmer’s rice field, divided into 3 sub-plot. Each sub-plot was planted with variety paddy of Inpari 22 (sub-plot A), Inpari 33 (sub-plot B), and Inpari 43 (sub-plot C). The number of cage traps was 6 traps per sub-plot. Observations were made 7 times, with an interval of 10 days. Results showed species of trapped rats in the research area was Rattus argentiventer, in which number of males was higher than females. Morphologically, the size of males were bigger than female. Number of footprints did not reperesentative the number of trapped rats. Inpari 43 variety was more preferred by rats because damage intensity was the highest than other two rice varieties.


Sign in / Sign up

Export Citation Format

Share Document