Isolation, Identification and Antioxidant Potential of Major Flavonoids from Ethyl Acetate Fraction of Torreya grandis

2013 ◽  
Vol 25 (5) ◽  
pp. 2459-2464
Author(s):  
M. Khalid Saeed ◽  
M. Naeem Khan ◽  
Ijaz Ahmad ◽  
Naqi Hussain ◽  
Sakhawat Ali ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Torreya Grandis

scholarly journals POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

2021 ◽  
Vol 45 ◽  
Author(s):  
Andréa Michel Sobottka ◽  
Elisandra Tessaro ◽  
Suelen Maier da Silva ◽  
Marina Pedron ◽  
Lara Tortini Seffrin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Butanol Fraction ◽  
Degraded Ecosystems

ABSTRACT Allophylus edulis and Cupania vernalis (Sapindaceae) are Brazilian native trees used as medicinal plants for the treatment of respiratory, digestive, circulatory, and skin diseases. Ubiquitously distributed in the Brazilian territory, these species are indicated for mixed plantations aimed at the recovery of degraded ecosystems. In this study, the total phenolic content (TPC) and total flavonoid content (TFC), and the antioxidant activity of extracts and fractions obtained from A. edulis and C. vernalis leaves were assessed. The TPC and TFC was determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts were obtained by two methods: maceration (method 1) and Soxhlet (method 2). Solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and n-butanol) were used to obtained the fractions. The results showed that the ethyl acetate fraction from A. edulis, obtained from the maceration method, had the highest TPC (442.0 ± 18.2 mg GAE g-1) and TFC (58.1 ± 0.4 mg RUE g-1), and antioxidant activity (EC50 = 43.6 ± 2.6 µg mL-1). By C. vernalis, superior results were obtained with the n-butanol fraction (TPC = 126.1 ± 5.8 mg GAE g-1, TFC = 37.7 ± 0.6 mg RUE g-1). The highest antioxidant potential was found in the crude hydroalcoholic extract (EC50 = 816.1 ± 50.9 µg mL-1) and butanol fraction (1,156.4 ± 3.8 µg mL-1). The results of this study show that the fractions obtained by maceration and liquid-liquid partition with more polar solvents (ethyl acetate and n-butanol) are the richest in TPC and TFC, and presented the greater antioxidant activity. Comparing the two plants, A. edulis showed the best results, with a high content of TPC, TFC, and antioxidant potential, and therefore may be used to treat diseases related to oxidative stress.

scholarly journals Antioxidant Potential of Crude Methanol Leaf extract and Fraction of Mallotus oppositifolius

2021 ◽  
pp. 168-184
Author(s):  
Ifeanyi Peter Onyeka ◽  
Felix Ahamefule Onyegbule ◽  
Christopher Obodike Ezugwu ◽  
Chibueze Jeremiah Ike ◽  
Chidalu B. Ikeotuonye
Keyword(s):  
Methyl Ester ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Acid Methyl

Mallotus oppositifolius (Geiseler) Mull. Arg.  (Euphorbiaceae) is a predominant edible shrub in Nigeria. It is locally used for treatment of common infections and wounds. This study evaluated the toxicity and antioxidant potential of the crude extract and fractions of M. oppositifolius using invitro 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging assay. The result showed abundance of flavonoids (28.21), tannins (17.72), alkaloids (7.79) and Saponins (0.23) in the crude extract of M. oppositifolius leaf extract. Also, the result showed that the LD50 is above 5000 mg/kg, therefore the crude extract is safe for consumption. The result further showed that the ethyl-acetate and butanol had significant (p<0.05) antioxidant activity when compared with the control at 125, 62.5 and 31.125 µg/ml respectively while at 250 and 500 µg/ml there was no significant different in the level of reduction of oxidation between the butanol and ethyl acetate when compared with the control. The result further showed that the butanol and ethyl-acetate fraction of M. oppositifolius had the best antioxidant potential among the fractions studied. The result of GCMS studies of ethyl-acetate fraction showed the presence of 7,10,13-eicosatrienoic acid methyl ester, korormicin, agosterol, phyllanthin, ricinoic acid, 2,4,6-decatrienoic acid 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9, limonin, com-pounds that have shown to possess strong antioxidant activities. Also, the GCMS result of butanol fraction showed the presence of seven compounds which include: ferulylhistamine, hippeastrine, 10,13-eicosadienoic acid methyl ester, ethyl 5,8,11,14-eicosatetraenoate, 6-hydroxy-powelline-N-nitroso-7-demethoxy-aldehyde, 6,7-epoxypregn-4-ene-9,11,18-triol-3,20-dione 11,18-diacetate and 5Alpha-androstane-3,17-dione 17-monooxime,  compounds which may serve as a new lead for the treatment of diseases associated with the oxidative stress.

scholarly journals Antibacterial Activity, Antioxidant Potential and Stigmasterol Isolation from Laggera aurita Linn (Asteraceae)

2021 ◽  
pp. 24-39
Author(s):  
C. Yohanna ◽  
A. Kwaji ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Crystalline Solid ◽  
Significant Activity ◽  
In Vitro Investigation

The study was aimed at the in-vitro investigation of the antibacterial activity, antioxidant potential and bioactive compound isolation from ethyl acetate crude fraction of Laggera aurita (L. aurita) Linn. The crude fraction was tested against five gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Proteus mirabilis) and three gram positive bacteria (Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis) using macro dilution technique. The antioxidant potentials were evaluated using two different but complementary methods namely ferrous ion chelating activity (FICA) and ferric reducing antioxidant potential (FRAP). Phytocompound isolation was carried out on low pressure open column chromatography. The crude fraction displayed moderate to significant activity against all tested bacteria exhibiting both bacteriostatic and bactericidal effects. The crude fraction showed Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in the range of 62.50-250 µg/mL and 125-1000 µg/mL respectively. The crude fraction was bactericidal to all tested pathogens except E. coli. The fifty percent effective concentration (EC50) of EDTA standard and the ethyl acetate fraction were obtained as 10.87 μg/mL and 25.77 μg/mL respectively. Similarly the FRAP determinations for ascorbic acid and ethyl acetate fraction yielded 153.63 and 134.40 Fe2+ Equivalent per g of fraction indicating 80.06% and 70.61% FRAP units respectively. The isolated phytocompound coded LAE was obtained as a white crystalline solid with melting point of 136‐138°C and Rf of 0.56 in hexane: ethylacetate (6:4). The isolated compound was identified by spectroscopic data analysis from FT-IR, GC-MS, 1D and 2D NMR and in comparison with literature. The compound was identified as stigmasterol. The observed significant antibacterial and antioxidant properties demonstrated by L. aurita in this study validate its widespread use in traditional medicine. Therefore the study had shown that L. aurita contains bioactive principles and may serve as a source for potential antibacterial and oxidative stress therapeutic agents.

scholarly journals Phenolic composition and antioxidant potential of different solvent extracts of the endemic Origanum elongatum (Bonnet) Emberger& Maire

2020 ◽  
Vol 10 (2) ◽  
pp. 146-154
Author(s):  
Imane Tagnaout ◽  
Hannou Zerkani ◽  
Sanae Amine ◽  
Kamal Fadili ◽  
Nadia Benhlima ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Screening Tests ◽  
Dpph Radical ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Health Disorders ◽  
Hydroethanolic Extract

Plants of the Lamiaceae family are widely used for medicinal, cosmetic and culinary purposes. Phenolic compounds such as flavonoids and tannins are the main constituents of these plants; they have a proven multidirectional biological activity. Polyphenols have exceptional antioxidant potential, and they can intervene in the prevention of many health disorders. This work aims to study the composition and antioxidant power of phenolic compounds from crude extracts and fractions of Origanum elongatum leaves. This specie was subjected to phytochemical study through precipitation, turbidity and coloring reactions to highlight their secondary metabolites qualitative composition. Polyphenolic extraction was made by Soxhlet using two solvents: methanol 70%, ethanol 70%. Then, methanol’s crude was fractionated with solvents having escalating polarity; ethyl acetate, n-butanol. The polyphenols and flavonoids contents were spectrophotometrically estimated using Folin-Ciocalteau and Aluminum Trichloride methods. The antioxidant power of crude and fractionated extracts was evaluated by diphenyl-picryl-hydrazyl (DPPH●) and iron (FRAP) tests. The screening tests showed the existence of polyphenols, mucilages, sterols and triterpenes, oses and holosides. The highest yield of extraction is obtained by 70% methanol, with an average of 32.29%. Quantitative assays indicated that the hydroethanolic extract and the ethyl acetate fraction possessed high amount of polyphenols and flavonoids compared with other fractions and subsequently exhibited a marked scavenging activity on DPPH radical (IC50 = 0,085±0,002 and 0,112±0,003 mg/ml, respectively) and high reductive ability on ferric ion assay with IC50 = 0,181±0,004 mg/ml, 0,291±0,005 mg/ml, respectively.

scholarly journals ANTIOXIDANT ACTIVITY OF TARENNA POLYCARPA (MIQ.) KOORD. LEAF

2018 ◽  
Vol 11 (4) ◽  
pp. 224
Author(s):  
Rika Puspita Sari ◽  
Marline Nainggolan ◽  
Rosidah Rosidah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Hexane Fraction ◽  
Dpph Assay

 Objective: The objective of this study is to evaluate the antioxidant activity of Tarenna polycarpa (Miq.) koord. Ex Valenton. Leaf extract and fractions.Methods: Antioxidant activity was examined by DPPH method.Results: Ethanol extract, n-hexane fraction, and ethyl acetate fraction with DPPH assay measured as half maximal inhibitory concentration were 55.21, 109.73, and 42.04 μg/mL, respectively.Conclusions: The results reveal that T. polycarpa extract and fractions have strong antioxidant potential. Our further study is to isolate compounds responsible for antioxidant components.

scholarly journals Simultaneous Detection and Validation of Analytical Markers of Swertia chirata by HPLC-DAD to Evaluate the Potency of Extracts and Fractions against Antioxidant Potential

2021 ◽  
Vol 33 (5) ◽  
pp. 977-982
Author(s):  
Deepak Singh Janoti ◽  
Kumud Upadhyaya
Keyword(s):  
Ethyl Acetate ◽  
Simultaneous Detection ◽  
Water Extract ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Dpph Assay ◽  
Frap Assay ◽  
Hydroalcoholic Extract ◽  
Abts Assay ◽  
Swertia Chirata

The present study is based on the selection of extract and fraction of Swertia chirata plant for the antioxidant potential with HPLC fingerprinting, which includes the simultaneous detection and quantification of four analytical markers protocatechuic acid (PCA), swertiamarin (SM), mangiferin (MF) and amarogentin (AG) by HPLC-DAD. The yield of water extract (SWA), hydroalcoholic extract (SHA) and fractions of hydroalcoholic extracts were evaluated for their antioxidant potential against 2,2-diphenyl-1-picrylhydrazyl-hydrate free radical assay (DPPH assay), 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid radical scavenging assay (ABTS assay), total reducing assay (TRA), ferric reducing antioxidant potential assay (FRAP assay), total antioxidant capacity assay (TAC assay). The hydroalcoholic extracts (SHA) can be a better choice as compared to water extract (SWA) due to higher yield of extract (13.680 ± 0.548%) and higher antioxidant activity against DPPH assay, ABTS assay, TRA assay, FRAP assay and TAC assay. In hydroalcoholic extract (SHA), ethyl acetate fraction (SEA) showed most potent activity against DPPH (IC50 = 0.008 ± 0.002 mg/mL) and ABTS (0.025 ± 0.001 mg/mL). n-Hexane fraction of SHA showed higher FRAP (28.664 ± 3.153 μmol/mL) and TAC (3.263 ± 0.325 μmol/mL) value (equivalent to ascorbic acid in μmol/mL) but showed very low yield (0.468 ± 0.018%), SBU showed higher TRA value (0.413 ± 0.309 mg/mL). The ethyl acetate fraction (SEA) can be a choice for antioxidant as it showed second highest FRAP (19.547 ± 2.119 μmol/mL) and TAC (2.750 ± 0.466 μmol/mL) with better yield (2.473 ± 0.594%) as compared to n-hexane (SH) fractions (0.468 ± 0.018%).

scholarly journals Assessment of In-Vivo Antioxidant Potential of Hydro-Alcoholic Extract and Ethyl Acetate Fraction of Aerva Javanica Linn. Flowering Tops

2020 ◽  
Vol 7 (6) ◽  
pp. 72-78
Author(s):  
Alok Khunteta ◽  
Surendra K Swarnkar ◽  
Manish Kumar Gupta ◽  
Aruna Swarnkar ◽  
Swapnil Sharma ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Catalase Activity ◽  
Centella Asiatica ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Alcoholic Extract ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Pharmacological Potential

Aerva javanica (Amaranthaceae) is a grey coloured woolly perennial tomentose shrub. Its traditional and folklore usage motivates further investigation on its pharmacognostic parameters and pharmacological potential. Hydro-alcoholic extract (AJCE) was prepared from flowering tops of A. javanica. In order to work further on activity guided fractions, ethyl acetate (AJEAF) fraction was prepared.  Therefore, in order to establish its antioxidant potential, in-vivo effect on LPO, GSH, SOD and catalase activity was determined. For comparison, silymarin and Centella asiatica extract (CAE) were used as standard antioxidant compound/extract. Lipid peroxidation in term of MDA content expressed as nM/mg, which was 82.18 and 67.39 for AJCE with increasing doses of complete hydro-alcoholic extract (AJCE represented as AJCE-1 and AJCE-2) and 51.65 for AJEAF in contrast to 40.64 nM/mg for standard silymarin and 46.81 nM/mg for standard CAE. GSH content was determined as 3.12, 3.82 and 4.56 μg/mg wet tissue in contrast to 5.59 for standard silymarin and 4.42 for standard CAE. Superoxide scavenging was expressed as SOD U/mg wet tissue, determined as 7.26, 9.16 and 9.91 U/mg wet tissue for AJCE-1 (250 mg/kg i.p. b.w), AJCE-2 (500 mg/kg i.p. b.w), and AJEAF respectively in comparison to silymarin (10.11) and CAE (46.81 U/mg wet tissue). Catalase activity expressed as μM of H2O2 decomposed / min / mg wet tissue was determined as 0.61, 0.72 and 0.78 repectively for AJCE-1 (250 mg/kg i.p. b.w), AJCE-2 (500 mg/kg i.p. b.w), and AJEAF. Results indicated the SOD values and total antioxidant power of DEE and EAF fractions even better than standard ascorbic acid which expressed the prospective potential of fractions (DEE and EAF) against metabolic disorders.  

scholarly journals SKRINING AKTIVITAS ANTIOKSIDAN FRAKSI EKSTRAK ETANOL KERNEL BIJI LIMUS

2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Vera Nurviana ◽  
Ade Yeni Aprilia ◽  
Eka Kartika Nuraini
Keyword(s):  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Seed Kernel ◽  
Standard Compound ◽  
Bioassay Guided Fractionation ◽  
Fractionation Method ◽  
Uv Visible

ABSTRAK Antioksidan merupakan suatu senyawa yang sangat penting dalam memelihara kesehatan. Salah satu bahan alam yang berkhasiat sebagai antioksidan adalah biji limus (Mangifera foetida L.) yang merupakan salah satu spesies mangga dari golongan anacardiaceae yang menyebar di wilayah Indonesia. Penelitian ini bertujuan untuk mengetahui fraksi yang memiliki aktivitas antioksidan yang paling baik pada kernel biji limus dengan metode Bioassay Guided Fractionation melalui reaksi penangkapan radikal bebas DPPH. Penyarian kernel biji buah limus dilakukukan menggunakan metode maserasi dengan pelarut etanol 96%. Ekstrak selanjutnya difraksinasi dengan pelarut n-heksana, etil asetat, metanol dan air. Aktivitas antioksidan masing-masing fraksi diukur menggunakan spekrofotometer UV-Visible. Hasil menunjukan bahwa fraksi etil asetat ekstrak etanol kernel biji limus memberikan potensi antioksidan yang paling baik yaitu dengan nilai ES50 sebesar 1,164±0.005 μg/mL (sangat kuat), melebih potensi vitamin C sebagai pembandingnya. Kata Kunci: Biji limus, Mangifera, Antioksidan, DPPH. ABSTRAK Antioxidants are very important compounds in maintaining health. One of the natural ingredients has efficacious as an antioxidant is Limus (Mangifera foetida L.) seeds which is one of the mango species from the Anacardiaceae group which spreads in the territory of Indonesia. This study aims to screen the fraction that has the high antioxidant potential of the Lotus seed kernel used Bioassay-Guided Fractionation method through scavenging reaction of DPPH as free radical. The extraction of the limus seeds was carried out using the maceration method with 96% ethanol. The extract was then fractionated with n-hexane, ethyl acetate, methanol, and water solvents. The antioxidant activity of each fraction was measured using a UV-Visible spectrophotometer. The results showed that the ethyl acetate fraction of the limus seed ethanol extract provided the high antioxidant potential with an ES50 value of 1.164 ± 0.005 μg / mL (very strong), exceeding the potential of vitamin C as a standard compound. Keywords: Limus seeds, Mangifera, Antioxidants, DPPH.

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