scholarly journals THE EFFECT OF PURIFIED AMOUNT OF TRITERPENE GLYCOSIDES AND THEIR ENRICHED EXTRACT FROM LEAVES OF SILPHIUM PERFOLIATUM L. ON THE GROWTH AND ACTIVITY OF NITRATE REDUCTASE OF WIN-TER WHEAT PLANTS

2019 ◽  
pp. 441-448 ◽  
Author(s):  
Eleonora Sergeyevna Davidyants
Keyword(s):  
Nitrate Reductase ◽  
Winter Wheat ◽  
Seed Treatment ◽  
Nitrate Reductase Activity ◽  
Growth Parameters ◽  
Reductase Activity ◽  
Dry Weight ◽  
Potassium Nitrate ◽  
Triterpene Glycosides ◽  
Nr Activity

The effect of seed treatment with solutions of a purified amount of triterpene glycosides (PATG) containing, as major components, oleanolic acid glycosides – sylphiosides B, C, E, G, and extract (E) enriched with sylphiosides, from Silphium perfoliatum L. (Asteraceae) leaves on growth parameters and nitrate reductase activity (NR, EC 1.6.6.1) of 7-day winter wheat plants (Tritium aestivum L.) was studied. It was shown that, seed treatment with PATG solutions in concentrations of 0.0005 and 0.001% and E in concentrations of 0.2 and 0.4% caused an increase in the length of roots, shoots, wet and dry weight of seedlings compared to the control. The stimulating effect of these concentrations of PATG and E on the total nitrate reductase activity of the roots and leaves of seedlings has been established, and an increase in the stimulating effect of preparations on the activity NR оf against the background of substrate activation of the enzyme potassium nitrate (KNO3) was observed. The greatest increase in the total NR activity of roots and leaves of winter wheat plants was observed when PATG acted at a concentration of 0.001% and E – at a concentration of 0.4%, which amounted respectively 122 and 116%, when adding 1 ml of 50 mM KNO3 solution into the growing medium of plants – 141 and 137% relative to the control. The stimulating effect of exogenous triterpene glycosides on NR activity has been established for the first time. The obtained data allow to theoretically substantiate the possibility of practical use of triterpene glycosides and preparations based on them for the regulation of growth and nitrogen metabolism of plants.

scholarly journals Effect of NaCI and supplemental calcium on growth parameters and nitrate reductase activity in maize

2011 ◽  
Vol 74 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Elżbieta Sacała ◽  
Agnieszka Biegun ◽  
Anna Demczuk ◽  
Edward Grzyś
Keyword(s):  
Nitrate Reductase ◽  
Nutrient Solution ◽  
Nitrate Reductase Activity ◽  
Growth Parameters ◽  
Reductase Activity ◽  
Dry Weight ◽  
Growth Responses ◽  
Positive Effects ◽  
Maize Varieties

In this study, investigated were the effects of NaCl (60 mmol/dm<sup>-3</sup>) and NaCl supplemented with different salts (5 mmol/dm<sup>-3</sup> CaCl<sub>2</sub>, CaSO<sub>4</sub>, CaCO<sub>3</sub>, KCl), on growth of two maize varieties (Cyrkon and Limko). After 7 days of cultivation in nutrient solution the growth response to salinity of both maize varieties was similar. NaCl led to a dramatic decrease in growth of plants (approx. 50% reduction in fresh and dry weight of root, and 70% reduction in fresh weight of shoot). Addition of extra Ca<sup>2+</sup> or K<sup>+</sup> to nutrient solution containing NaCl did not definitely improve the growth parameters of maize. However, among the tested salts, CaCl<sub>2</sub> had a beneficial visual effect on maize seedlings. In other cases the plants showed noticeable symptoms of salt damage. In long term exposure to salinity (two weeks) growth of Cyrkon was more inhibited than Limko. Comparison of growth responses in short-term exposure to salinity (7 days) with long-term (14 days) showed that in Cyrkon variety the negative effects of NaCl were intensified and addition of CaCl<sub>2</sub> to salinized solution had not positive effects on growth. On the contrary, in Limko variety, there was a significant improvement in growth (especially in root dry weight). This fact indicates that during longer exposure to salinity Limko was able to adapt to those conditions. Salinity caused a significant decrease in leaf nitrate reductase activity (60% and 30% reduction respectively in Limko and Cyrkon). Addition of CaCl<sub>2</sub> to salinized nutrient solution resulted in greater enzyme inhibition in Cyrkon (50% decline in relation to plants grown under sole NaCl), and 30% increase in Limko. Inhibition of nitrate reductase activity did not cause a decrease in concentration of soluble protein in maize leaves.

INFLUENCE DU CLIMAT SUR L’ACTIVITÉ NITRATE RÉDUCTASE AU COURS DU DÉVELOPPEMENT DE SIX VARIÉTÉS D’AVOINE

1989 ◽  
Vol 69 (3) ◽  
pp. 919-923 ◽  
Author(s):  
A. SAUVESTY ◽  
G. GENDRON
Keyword(s):  
Environmental Factors ◽  
Nitrate Reductase ◽  
Plant Development ◽  
Significant Influence ◽  
Environmental Conditions ◽  
Nitrate Reductase Activity ◽  
Nitrate Nitrogen ◽  
Reductase Activity ◽  
Stage Of Development ◽  
Nr Activity

The influence of field climate on nitrate reductase activity (NR) and development was studied in six oat varieties. Of the monitored environmental factors, only temperature had a significant influence; a temperature increase accelerated plant development and increased NR. It was found that a measure of NR activity as early as the coleoptile stage of development indicated optimal environmental conditions for reduction of nitrate nitrogen in a given genotype.Key words: Nitrate reductase activity, development, climate, oat

INFLUENCE DE LA TEMPERATURE DU SUBSTRAT ET DE LA FERTILISATION AZOTEE SUR LA CROISSANCE, LE DEVELOPPEMENT, LA TENEUR EN AZOTE ET L’ACTIVITE DE LA NITRATE REDUCTASE CHEZ LA TOMATE

1984 ◽  
Vol 64 (1) ◽  
pp. 181-191 ◽  
Author(s):  
ANDRÉ GOSSELIN ◽  
FRANÇOIS-P. CHALIFOUR ◽  
MARC J. TRUDEL ◽  
GHISLAIN GENDRON
Keyword(s):  
Lycopersicon Esculentum ◽  
Nitrate Reductase ◽  
Nitrogen Content ◽  
Nitrate Reductase Activity ◽  
Root Zone ◽  
Reductase Activity ◽  
Dry Weight ◽  
Fruit Weight ◽  
Root Zone Temperature ◽  
Lycopersicon Esculentum Mill

We measured the effects of five root temperatures (12, 18, 24, 30 and 36 °C) and five rates of nitrogen fertilization (0, 2.5, 7.5, 22.5 and 67.5 meq N∙L−1) on growth, development, nitrogen content and nitrate reductase activity of tomato plants (Lycopersicon esculentum Mill. ’Vendor’). The greatest root dry weight was obtained at 18 °C and 2.5 meq N∙L−1 and the highest shoot dry weight at 24 °C and 22.5 meq N∙L−1. Total fruit weight was maximum at 24 °C and 2.5 meq N∙L−1. High root temperatures and high levels of fertilization increased the number of aborted flowers and the nitrogen content of shoots, but reduced tomato yields. Nitrate reductase activity (NRA) was higher in leaves than in roots and with plants receiving 22.5 meq N∙L−1 as compared to 2.5 meq. NRA in roots decreased as temperature increased.Key words: Lycopersicon esculentum Mill., root zone temperature, nitrate reductase, nitrogen

scholarly journals Treatment of soybean seeds with molybdenum and inoculant: nitrate reductase activity and agronomic performance

2019 ◽  
Vol 10 (1) ◽  
pp. 186-194
Author(s):  
Ewerton Gewehr ◽  
Otávio De Oliveira Corrêa ◽  
Anna Dos Santos Suñé ◽  
Gabriel Bandeira Duarte ◽  
Luciano Do Amarante ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Nitrate Reductase ◽  
Seed Treatment ◽  
Nitrate Reductase Activity ◽  
Seed Quality ◽  
Agronomic Traits ◽  
Reductase Activity ◽  
Seedling Emergence ◽  
Seed Vigour ◽  
Presence And Absence

The aim of this study was to analyze the effects of molybdenum and inoculant application via seed treatment in soybean, and their role in the nitrate reductase enzyme activity, agronomic traits and physiological quality of the produced seeds. The experiment was conducted at the Federal University of Pelotas (UFPel), Rio Grande do Sul state, Brazil. The treatments were shaped by five doses of molybdenum (zero; 16; 32; 48; 64 g.100kg-1 of seeds) combined with the presence and absence of liquid inoculant (Bradyrhizobium japonicum), at a dose of 200 mL per 100 kg of seeds. The evaluations of nitrate reductase activity, agronomic traits and physiological seed quality were performed in the present work. The measured agronomic traits were: plant height, number of pods per plant, number of seed per plant and thousand-seed weight. Standard germination test was executed to evaluate the produced seed viability. First germination counting, accelerated aging test, cold test, plantlet shoot and root length, plantlet shoot and root dry mass, and field seedling emergence were utilized to measure the seed vigour. Molybdenum, both in presence and absence of inoculant, positively influenced the enzymatic activity, for both vegetative and reproductive stages. The addition of inoculant and molybdenum provided increase in the vigour of the produced seeds. The enzyme activity was positively correlated with the agronomic traits and vigour tests. The addition of inoculant and molybdenum in the seed treatment provides a better expression of nitrate reductase activity and vigour of the produced seeds.

scholarly journals Effects of Aquatic Extract from Chrysanthemum's Rhizospheric Soil on the Seed Germination and Root Enzymes

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 789E-789
Author(s):  
Kai Zhou ◽  
Weiming Guo ◽  
Zhongchun Jiang*
Keyword(s):  
Seed Germination ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Water Extract ◽  
Dry Weight ◽  
Rhizospheric Soil ◽  
Malondialdehyde Content

The autointoxication of chrysanthemum was studied using water extract of Dendranthema morifolium's rhizospheric soil. Results of bioassays showed that the water extract inhibited chrysanthemum seed germination and the activities of some important root enzymes. The seedling nitrate reductase activity was decreased linearly with increasing concentration of the extract. The activity of root dehydrogenase was inhibited only at the highest concentration tested [3.2 g·mL-1, dry weight (DW)], but was stimulated at a lower concentration tested (1.6 g·mL-1, DW). Malondialdehyde content increased at higher than 1.6 g·mL-1, DW concentrations of the extract. The autointoxication phenomenon might be related to the difficulties in continuous plantings of chrysanthemum at the same location.

scholarly journals Effects of growth retarding compounds on chlorophyll accumulation and nitrate reductase activity in nitrate induced cucumber cotyledons

2015 ◽  
Vol 42 (3) ◽  
pp. 431-439 ◽  
Author(s):  
J. S. Knypl
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Chlorophyll Synthesis ◽  
Growth Retardants ◽  
Chlorophyll Accumulation ◽  
High Concentrations ◽  
Amo 1618 ◽  
Nr Activity

Cotyledons were excised from 5-day old etiolated cucumber seedlings and .grown for 24 or 48 h in solutions of plant growth retardants: AMO-1618,B-Nine, CCC and phosfon D, supplemented with KNO<sub>3</sub> (10<sup>-2</sup>M) in light. Nitrate reductase (NR) activity was determined <i>in vivo</i>. CCC and Phosfon D at high concentrations had no effect on nitrate reductase activity in 24 h tests. CCC at 5xl0<sup>-2</sup> M enhanced NR activity in longer 48 h tests; Phosfon D was inhibitory in that case. AMO-1618 markedly decreased NR activity. B-Nine strikingly enhanced NR activity in KNO<sub>3</sub> induced cytoledons; the effect was positively correlated with the concentration of B-Nine. Ali the compounds inhibited chlorophyll synthesis.

Ecophysiological responses of Araucaria angustifolia seedlings to different irradiance levels.

2000 ◽  
Vol 48 (4) ◽  
pp. 531 ◽  
Author(s):  
Leandro da S. Duarte ◽  
Lúcia R. Dillenburg
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Growth Parameters ◽  
Reductase Activity ◽  
Chlorophyll Concentration ◽  
Shoot Biomass ◽  
Araucaria Angustifolia ◽  
Low Light ◽  
Pine Seedlings ◽  
Ecophysiological Responses

Brazilian pine (Araucaria angustifolia [Bert.] O.Ktze.) is an indigenous conifer of the southern region of South America, typically regarded as a sun-loving species. However, there is insufficient information on the species’ behaviour under different irradiances to characterise its plasticity to light. The purpose of this work was to measure some ecophysiological responses of Brazilian pine seedlings to three irradiance levels: 100, 45 and 10% full sunlight. Thirty seedlings were grown under each of the irradiance treatments and, after 5 months, growth parameters, leaf chlorophyll concentration and in vivo nitrate reductase activity, in both leaves and roots, were analysed. There was no increase in shoot biomass allocation in response to shading, in contrast to results from other experiments. Only low-light seedlings showed significant increase in specific leaf area, as well as in the ratio of height to shoot biomass. Chlorophyll content and the ratio of chlorophyll a to chlorophyll b did not vary among treatments. Nitrate reductase activity was detected only in roots and was reduced in the low-light treatment. An overall analysis of the results points to a normal growth of Brazilian pine seedlings at both high- and medium-light sites, at least in their initial stages of development.

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