scholarly journals Adaptação de um bioensaio simplificado para avaliação do status de susceptibilidade em larvas de Anopheles darlingi e Anopheles marajoara ao piretroide deltametrina

BioAssay ◽  
2014 ◽  
Vol 9 ◽  
Author(s):  
Ana Paula Barbosa Silva ◽  
Wallison Sousa Alves ◽  
Ademir Jesus Martins Junior ◽  
Wanderli Pedro Tadei ◽  
Joselita Maria Mendes Santos
Keyword(s):  
Anopheles Darlingi

scholarly journals Registro de Culicidae de importância epidemiológica na área rural de Manaus, Amazonas

2008 ◽  
Vol 41 (6) ◽  
pp. 658-663 ◽  
Author(s):  
Maria das Graças Vale Barbosa ◽  
Nelson Ferreira Fé ◽  
Alexandre Herculano Ribera Marcião ◽  
Ana Paula Thomé da Silva ◽  
Wuelton Marcelo Monteiro ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Anopheles Darlingi

A biodiversidade da Região Amazônica inclui diversas espécies de vetores artrópodes em seus diferentes ecótopos, possibilitando o surgimento de doenças como malária, filarioses e arboviroses. De agosto de 2001 a julho de 2002, realizou-se coletas de culicídeos no domicílio, peridomicílio e nas matas da Comunidade São João, área rural de Manaus, Amazonas. Foram capturados 1.240 culicídeos, pertencentes às subfamílias Culicinae (99%) e Anophelinae (1%), somando 50 espécies. O predomínio entre as tribos foi nitidamente de Culicini, com 904 (72,9%) exemplares, destacando-se as espécies Culex usquatus (22,6%) e Culex quinquefasciatus (17,7%). Do total de culicídeos, 1.077 (86,9%) exemplares foram capturados no interior das matas, 101 (8,1%) no peridomicílio e 62 (5%) no intradomicílio. O ecótopo com maior diversidade de espécies foi a mata. Assinalou-se a presença de Anopheles darlingi, Anopheles triannulatus, Aedes aegypti, Haemagogus janthinomys e outros vetores comprovados ou potenciais.

Anopheles darlingi versus Nyssorhynchus darlingi, the discussion continues

2021 ◽  
Author(s):  
Carlos J.E. Lamas ◽  
Silvio S. Nihei ◽  
Peter G. Foster ◽  
Jan E. Conn ◽  
Maria A.M. Sallum
Keyword(s):  
Anopheles Darlingi

scholarly journals Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Elerson Matos Rocha ◽  
Osvaldo Marinotti ◽  
Deidre Machado Serrão ◽  
Laura Viana Correa ◽  
Ricardo de Melo Katak ◽  
...  
Keyword(s):  
Public Health Problem ◽  
Major Public Health Problem ◽  
Culturable Bacteria ◽  
Anopheles Darlingi ◽  
Next Generation ◽  
Bacterial Strains ◽  
Promising Alternative ◽  
Breeding Sites ◽  
Adult Females ◽  
Microbiological Techniques

Abstract Background Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans. Methods Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates. Results A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation. Conclusion Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature.

scholarly journals Effects of flooding of the River Paraná on the temporal activity of Anopheles (Nyssorhynchus) darlingi Root (Diptera: Culicidae), at the border state of Mato Grosso do Sul and São Paulo, Brazil

2010 ◽  
Vol 43 (5) ◽  
pp. 516-522 ◽  
Author(s):  
Almério de Castro Gomes ◽  
Marcia Bicudo de Paula ◽  
Delsio Natal ◽  
Sabina Léa Davidson Gotlieb ◽  
Luis Filipe Mucci
Keyword(s):  
Activity Patterns ◽  
Electrical Energy ◽  
Sao Paulo ◽  
Malaria Vectors ◽  
Hydroelectric Power Station ◽  
São Paulo ◽  
Anopheles Darlingi ◽  
Hydroelectric Power ◽  
Mato Grosso ◽  
Mato Grosso Do Sul

INTRODUCTION: Study of the temporal activity of malaria vectors during the implantation of a hydroelectric power station on the River Paraná, intended to generate electrical energy. The river separates the States of São Paulo and Mato Grosso do Sul, in Brazil. The objective was to verify whether alterations occurred in the wealth and diversity indices of Anopheles, following two successive floods, extended to the temporal activity and nycthemeral rhythm followed over a five year period. METHODS: Mosquito capture was performed monthly using the Human Attraction Technique and Shannon Traps. The first, executed for 24h, provided the nycthemeral rhythm and the second, lasting 15h, permitted the tracking of Anopheles during the two floods. RESULTS: The bimodal pattern of Anopheles darlingi defined before these floods was modified throughout the environment interventions. The same effect had repercussions on the populations of An albitarsis s.l., An triannulatus and An galvaoi. Activity prior to twilight was less affected by the environment alterations. CONCLUSIONS: The dam construction provoked changes in Anopheles temporal activity patterns, permitting classification of the area as an ecologically steady and unstable situation. Differences observed in Anopheles behavior due to the capture methods revealed the influence of solo and multiple attractiveness inside the populations studied.

scholarly journals Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus

2016 ◽  
Vol 111 (5) ◽  
pp. 335-346 ◽  
Author(s):  
Anthony J Cornel ◽  
Katherine K Brisco ◽  
Wanderli P Tadei ◽  
Nágila FC Secundino ◽  
Miriam S Rafael ◽  
...  
Keyword(s):  
Population Structure ◽  
Polytene Chromosomes ◽  
Anopheles Darlingi

scholarly journals Intrapopulational genetic differentiation in Anopheles (N.) darlingi Root, 1926 (Diptera: Culicidae) in the amazon region

1999 ◽  
Vol 22 (3) ◽  
pp. 325-331 ◽  
Author(s):  
Joselita Maria Mendes dos Santos ◽  
Jorge Arturo Lobo ◽  
Wanderli Pedro Tadei ◽  
Eucleia P. Betioli Contel
Keyword(s):  
Genetic Differentiation ◽  
Central Region ◽  
Amazon Basin ◽  
Amazon Region ◽  
Anopheles Darlingi ◽  
Chromosomal Variation ◽  
Geographical Differentiation ◽  
Marginal Populations ◽  
Polymorphic Loci

Four natural Amazonian Anopheles darlingi populations were analyzed for 19 loci, 12 of which showed variation, i.e., 68.4% of polymorphic loci. The Ariquemes, Rondônia, population was the most variable, with a large number of alleles per locus (2.26 ± 0.27) and high polymorphism (P = 63.15). The highest values of observed and expected intralocus heterozygosity were observed in the Manaus, Amazonas, population (Ho = 0.432 ± 0.11; He = 0.375 ± 0.08), and the lowest in the Cachoeira Porteira, Pará, population (Ho = 0.236 ± 0.09; He = 0.290 ± 0.11). Wright's F-statistic revealed disequilibrium caused by an excess of homozygotes, as shown by the Fis > Fst values (Fis = 0.083 > 0.026) reflecting intrapopulational differentiation. The four populations studied were genetically similar, as indicated by distance and similarity values. Chromosomal variation of Amazon population also did not indicate geographical differentiation, and populations in the central region of the Amazon Basin showed high polymorphism in relation to the marginal populations, which were mainly monomorphic.

LEVEDURAS DO TRATO DIGESTÓRIO DE Anopheles darlingi COMO ALTERNATIVA PARA O DESENVOLVIMENTO DE PARATRANSGÊNESE PARA O CONTROLE DA MALÁRIA

2020 ◽  
pp. 54-65
Author(s):  
Andrelisse Arruda ◽  
Antonio dos Santos Júnior ◽  
Gabriel Eduardo Melim Ferreira ◽  
Juliana Conceição Sobrinho ◽  
Luiz Shozo Ozaki ◽  
...  
Keyword(s):  
Anopheles Darlingi

scholarly journals Characterization of Bacterial Communities in Breeding Waters of Anopheles darlingi in Manaus in the Amazon Basin Malaria-Endemic Area

2019 ◽  
Vol 78 (4) ◽  
pp. 781-791 ◽  
Author(s):  
Louise K. J. Nilsson ◽  
Marta Rodrigues de Oliveira ◽  
Osvaldo Marinotti ◽  
Elerson Matos Rocha ◽  
Sebastian Håkansson ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Endemic Area ◽  
Amazon Basin ◽  
Anopheles Darlingi ◽  
Malaria Endemic Area
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