scholarly journals S1304 Intestinal Cell Culture Model System to Test the Effect of Natural Products on Drug Transport: Effect of Naringin on the Transport of Digoxin

2020 ◽  
Vol 115 (1) ◽  
pp. S654-S655
Author(s):  
Vaishali H. Oza ◽  
Natalya I. Motyka ◽  
Jacob P. Bitoun ◽  
Diane A. Blake
Keyword(s):  
Cell Culture ◽  
Natural Products ◽  
Drug Transport ◽  
Model System ◽  
Intestinal Cell ◽  
Cell Culture Model ◽  
Transport Effect ◽  
Culture Model

Investigation into the metabolism of 1,8-cineole in an intestinal cell culture model and acquisition of its immune-modulatory effect via gene expression analysis

2012 ◽  
Vol 27 (6) ◽  
pp. 405-413 ◽  
Author(s):  
Jakob Müller ◽  
Natalie Gruner ◽  
Isabella Almstätter ◽  
Frauke Kirsch ◽  
Andrea Buettner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Intestinal Cell ◽  
Cell Culture Model ◽  
Culture Model

Correlation between enzyme activity and substrate storage in a cell culture model system for Gaucher disease

2004 ◽  
Vol 27 (5) ◽  
pp. 649-658 ◽  
Author(s):  
U. H. Schueler ◽  
T. Kolter ◽  
C. R. Kaneski ◽  
G. C. Zirzow ◽  
K. Sandhoff ◽  
...  
Keyword(s):  
Cell Culture ◽  
Enzyme Activity ◽  
Gaucher Disease ◽  
Model System ◽  
Cell Culture Model ◽  
Culture Model ◽  
A Cell

scholarly journals Claudin-4 forms a paracellular barrier, revealing the interdependence of claudin expression in the loose epithelial cell culture model opossum kidney cells

2012 ◽  
Vol 303 (12) ◽  
pp. C1278-C1291 ◽  
Author(s):  
Jelena Borovac ◽  
Reid S. Barker ◽  
Juraj Rievaj ◽  
Andrew Rasmussen ◽  
Wanling Pan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ussing Chamber ◽  
Model System ◽  
Cell Culture Model ◽  
Opossum Kidney ◽  
Leaky Epithelia ◽  
Ok Cells ◽  
Cation Selectivity ◽  
Culture Model ◽  
Tightly Coupled

The effect of claudins on paracellular fluxes has been predominantly studied in either Madin-Darby canine kidney (MDCK) or LLCPK cells. Neither model system has a very low transepithelial resistance (TER) as observed in leaky epithelia. Moreover, results from one model system are not always consistent with another. Opossum kidney (OK) cells form tight junctions yet have a very low TER. We therefore set out to characterize the paracellular transport properties of this cell culture model. Ussing chamber dilution potential measurements revealed that OK cells exhibit a very low TER (11.7 ± 1.4 Ω·cm2), slight cation selectivity ( PNa/ PCl = 1.10 ± 0.01), and the Eisenman permeability sequence IV; the permeability of monovalent cations ranking K+ > Cs+ > Rb+ > Na+ > Li+. Quantitative real-time PCR studies found that OK cells endogenously express claudin-4 > -1 > -6 > -20 > -9 > -12 > -11 > -15. Overexpression of claudin-4 significantly increased TER, decreased Na+ and Cl− permeability, and increased levels of claudin-1, -6, and -9 mRNA. Knockdown of claudin-4 in the overexpressing cells significantly decreased TER without altering claudin expression; thus claudin-4 forms a barrier in OK cells. Knockdown of endogenous claudin-4 decreased claudin-1, -9, and -12 expression without altering TER. Claudin-2 overexpression decreased TER, significantly increased Na+ and Cl− permeability, and decreased claudin-12 and -6 expression. Together these results demonstrate that claudin expression is tightly coupled in OK cells.

Effects of zinc on epithelial barrier properties and viability in a human and a porcine intestinal cell culture model

2013 ◽  
Vol 27 (2) ◽  
pp. 834-843 ◽  
Author(s):  
U. Lodemann ◽  
R. Einspanier ◽  
F. Scharfen ◽  
H. Martens ◽  
A. Bondzio
Keyword(s):  
Cell Culture ◽  
Barrier Properties ◽  
Intestinal Cell ◽  
Epithelial Barrier ◽  
Cell Culture Model ◽  
Culture Model

The evaluate and compare the effects of the Tacrolimus and Sirolimus on the intestinal system using an intestinal cell culture model

2015 ◽  
Vol 26 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Hanen Ferjani ◽  
Haila El Abassi ◽  
Intidhar Ben Salem ◽  
Yosra Guedri ◽  
Salwa Abid ◽  
...  
Keyword(s):  
Cell Culture ◽  
Intestinal Cell ◽  
Cell Culture Model ◽  
Culture Model
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