Application of derivatives of UV spectra and similarity indices to identification of milk proteins separated using reversed-phase high performance liquid chromatography

Polimery ◽  
2003 ◽  
Vol 48 (02) ◽  
pp. 106-110 ◽  
Author(s):  
PIOTR MINKIEWICZ ◽  
JERZY DZIUBA ◽  
DOROTA NALECZ
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Milk Proteins ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Similarity Indices ◽  
Derivatives Of

Second and Third Derivatives of UV Spectra as a Tool for Identification of Major Anthocyanins from Aronia melanocarpa Extract, Separated Using Reversed-Phase High-Performance Liquid Chromatography

2004 ◽  
Vol 69 (7) ◽  
pp. 1443-1452 ◽  
Author(s):  
Piotr Minkiewicz ◽  
Barbara Pliszka ◽  
Jerzy Dziuba ◽  
Jan Oszmiański
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Uv Spectroscopy ◽  
Statistical Significance ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Correct Identification ◽  
Black Chokeberry ◽  
Derivatives Of

The aim of the study was to propose a method for identification of major anthocyanins in black chokeberry: cyanidin 3-O-β-D-galactopyranoside and cyanidin 3-O-α-L-arabinopyranoside, using reversed-phase high-performance liquid chromatography on line with UV spectroscopy. The similarity indexes between standard spectra and spectra of major components of black chokeberry extract were calculated. The calculations were performed for zero-order spectra and first-fifth derivatives. Only the second and third derivatives of UV spectra provided correct identification of the compounds investigated. Third derivatives of spectra gave higher values of the parameters (calculated using Student's test) indicating statistical significance between similarity indexes to the derivatives of spectra of anthocyanin standards. Third derivative UV spectroscopy may be thus recommended as a tool to identify anthocyanins.

Separation and Determination of Some Aromatic Sulfones by Reversed-Phase High-Performance Liquid Chromatography

1994 ◽  
Vol 59 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Josef Královský ◽  
Marta Kalhousová ◽  
Petr Šlosar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Optimum Conditions ◽  
Linear Calibration ◽  
Technical Grade

The reversed-phase high-performance liquid chromatography of some selected, industrially important aromatic sulfones has been investigated. The chromatographic behaviour of three groups of aromatic sulfones has been studied. The optimum conditions of separation and UV spectra of the sulfones and some of their hydroxy and benzyloxy derivatives are presented. The dependences of capacity factors vs methanol content in mobile phase are mentioned. The results obtained have been applied to the quantitative analysis of different technical-grade samples and isomer mixtures. For all the separation methods mentioned the concentration ranges of linear calibration curves have been determined.

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