scholarly journals Characteristics of Libido and Testosterone Concentration of Polled and Horned Bali Bulls after GnRH Injection

2021 ◽  
Vol 26 (3) ◽  
pp. 108
Author(s):  
Hasbi Hasbi ◽  
Herry Sonjaya ◽  
Sudirman Baco ◽  
Reski Amalia ◽  
Sri Gustina
Keyword(s):  
Blood Plasma ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Testosterone Concentration ◽  
Releasing Hormone ◽  
Gonadotrophin Releasing Hormone ◽  
Bali Cattle ◽  
First Time

Bali cattle are one of the original Indonesian germplasm, which in its development were found to be hornless or polled. Polled are Balinese cattle whose horns do not grow naturally, but have the same characteristics as those with horns. However, there are indications that polled have a lower libido. The purpose of this study was to determine the characteristics of libido and testosterone concentration after gonadotrophin releasing hormone (GnRH) injection. In this study each of 7 male polled and horned Bali cattle aged 3.5-6 years were used. Libido characteristics were observed by recording the first time the male fondled the teaser until ejaculation, while the testosterone concentration was measured using blood plasma taken on day 0 or before injection, day 7<sup>th</sup>, and day 14<sup>th</sup> after GnRH injection. Testosterone analysis used enzyme linked immunosorbent assay (ELISA) method. The results showed that the libido of polled was not different (P&gt;0.05) compared to that of the horned on the 0, 7<sup>th</sup> and 14<sup>th</sup> days after GnRH injection. However, in polled on the 7<sup>th</sup> day after GnRH injection, it was significantly lower than on day 0 and 14, but on day 0 it was not different with day 14. Testosterone concentration on day 7 after injection of GnRH was not different (P&gt;0.05) compared to day 0 both in polled and horned bulls, but on day 14 after injection was significantly higher (P&lt;0.05) in horned than polled. In conclusion, polled and horned Bali bulls had high libido with a score of +1, but on the 7<sup>th</sup> day after GnRH injection, polled had lower libido than horns. The testosterone concentrations of polled and horned on day 7 after GnRH injection were not different compared to day 0, while the 14<sup>th</sup> day was higher in horned bull.

scholarly journals Impact of environmental factors on growth and satratoxin G production by strains of Stachybotrys chartarum

2012 ◽  
Vol 5 (1) ◽  
pp. 37-43 ◽  
Author(s):  
S. Frazer ◽  
J. Pestka ◽  
J. Kim ◽  
A. Medina ◽  
D. Aldred ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Water Availability ◽  
Indoor Environment ◽  
Potato Dextrose Agar ◽  
Enzyme Linked Immunosorbent Assay ◽  
Stachybotrys Chartarum ◽  
Data Sets ◽  
Elisa Method ◽  
Contour Maps ◽  
First Time

The black mould Stachybotrys chartarum and its mycotoxins have been linked to damp building-associated illnesses. The objective of this study was to determine the effects of water availability (water activity, aw) and temperature on growth and production of satratoxin G (SG) by a macrocyclic trichothecene-producing strain (IBT 7711) and non-producing strain (IBT 1495) of S. chartarum. Growth studies were carried out on potato dextrose agar modified with glycerol to 0.995-0.92 aw at 10-37 °C. Growth extension was measured and the cultures were extracted after 10 days and a competitive enzyme-linked immunosorbent assay (ELISA) method used to quantify the SG content. Growth was optimal at 25 to 30 °C at 0.995 aw, but this was modified to 0.98 aw at 30 °C for both strains (1.4-1.6 mm/day, respectively). The ELISA method revealed that, in contrast to growth, SG production was maximal at 20 °C with highest production at 0.98 aw (approximately 250 μg/g mycelia). When water was freely available (0.995 aw), SG was maximally produced at 15 °C and decreased as temperature was increased. Interestingly, the strain classified as a non-toxigenic produced very low amounts of SG (<1.6 μg/g mycelia) that were maximal at 25 °C and 0.98 aw. Contour maps for growth and SG production were developed from these data sets. These data have shown, for the first time, that growth and SG production profiles are very different in relation to key environmental conditions in the indoor environment. This will be very useful in practically determining the risk from exposure to S. chartarum and its toxins in the built environment.

An enzyme-linked immunosorbent assay for the detection of apple mosaic virus in yellow birch

1980 ◽  
Vol 10 (3) ◽  
pp. 278-283 ◽  
Author(s):  
T. Hardcastle ◽  
A. R. Gotlieb
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Leaf Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Yellow Birch ◽  
Field Samples

An enzyme-linked immunosorbent assay (ELISA) method was developed to detect apple mosaic virus (ApMV) in Betulaalleghaniensis Britton. Tests for virus using bark and bud tissues of dormant trees were successful. ApMV was also detectable in old leaf tissue in August and September, as well as in newly emerging leaf tissue forced in a greenhouse in March. Whole crude antiserum used to coat ELISA plates in tests with bud tissues was a reliable substitute for purified immunoglobulin without loss of sensitivity or specificity. An attempt was made to use ELISA for quantifying virus concentrations in field samples of ApMV-infected birch leaves.

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay for Serotyping Ureaplasma urealyticum Strains Using Monoclonal Antibodies

2001 ◽  
Vol 8 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Fedoua Echahidi ◽  
Gaëtan Muyldermans ◽  
Sabine Lauwers ◽  
Anne Naessens
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Ureaplasma Urealyticum ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Cross Reactions ◽  
Antigenic Preparation ◽  
Negative Controls ◽  
Reference Strains

ABSTRACT Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to enable the serotyping of U. urealyticum isolates from primary broth cultures. Each of the 14 serotype reference strains was tested against 14 selected MAbs. Homologous reactions were very strong, while heterologous reactions were negligible. Three cross-reactions were observed: MAb 5 cross-reacted with serotype 2, MAb 14 cross-reacted with serotype 3, and MAb 8 cross-reacted with serotype 13. Despite the cross-reactions observed, all the serotype reference strains ofU. urealyticum could be identified and differentiated using a combination of MAbs. Reproducibility was analyzed with a fractionated antigenic preparation and with several freshly prepared antigens of the same strain. No significant interrun variation was found with the fractionated antigen, but significant variations in optical density (OD) values were found when freshly prepared antigens were tested. However, the variation in OD values did not influence the overall interpretation of the ELISA: reactions with homologous MAbs were always prominent compared to those of the negative controls. This newly developed ELISA using MAbs seems promising for serotyping of U. urealyticum clinical isolates.

scholarly journals Bovine Immunoglobulin E Levels of Bali Cattlesin Bangli and Nusa Penida island Bali Province, Indonesia

2019 ◽  
Vol 2 (2) ◽  
pp. 64
Author(s):  
Ni Ketut Suwiti ◽  
Luh Gde Surya Heryani ◽  
Desak Nyoman Dewi Indira Laksmi ◽  
Ni Nyoman Werdi Susari ◽  
I Nengah Kerta Besung ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Commercial Enzyme ◽  
Elisa Kit ◽  
Bali Cattle ◽  
Bovine Immunoglobulin

The aim of this research was to detect identify levels of Bovine Immunoglobulin E (BoIg.E), can be used as an indicator of response immune in bali cattle.  Eighty serum samples were collected from Nusa Penida and Bangli region. Bovine Ig.E levels was measured using a commercial Enzyme Linked Immunosorbent Assay (ELISA) Kit. The data were analysis based on differences of farming characteristics andgeographic. The result of research that, of BoIg.E level of bali cattle kept in Bangli (34.16258 ?g/ml), was higher than Nusa Penida (22.26047 ?g/ml). We conclude that there was a significant effect of differences of farming characteristics and geographic conditions.

scholarly journals SOME RESULTS OF RESEARCH WORK ON PREVENTION OF ECHINOCOCCOSIS IN KYRGYZSTAN

2020 ◽  
pp. 428-431
Author(s):  
Tursunov ◽  
Isaev ◽  
Ibragimova
Keyword(s):  
Veterinary Medicine ◽  
Immunosorbent Assay ◽  
Research Work ◽  
Preventive Treatment ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Outcomes ◽  
Kyrgyz Republic ◽  
Laboratory Studies ◽  
Elisa Method ◽  
Almost All

The Kyrgyz Research Institute of Veterinary Medicine is a co-executor of the Strategy for the Echinococcosis Control (2013–2018) and monitored dogs epizootological examinations after preventive treatment with azinox. Laboratory studies of biomaterials (feces) for infection with helminths were carried out along with the traditional coprological method, a more sensitive enzyme-linked immunosorbent assay (ELISA). The results of laboratory studies of fecal samples from dogs showed that the activities of the "Strategy for the Control of Echinococcosis in the Kyrgyz Republic", including the treatment of dogs with azinox, gave positive outcomes. From 2014 to 2018 2260 feces samples from deworming dogs from almost all regions of Kyrgyzstan were examined. At the same time, the infection of dogs with taeniidae is steadily decreasing; it has also been noted that the main joints of parasitocenosis in dogs are taeniidae, toxocaras, mesocestoids, and eimeria. In the studies conducted on the infection of dogs with taeniidae, the coprological and more sensitive ELISA method was used. Using both helminthological studies, a decrease in the invasion of dogs by taeniidae was confirmed. Thus, in the period from 2014 (the beginning of the Strategy) to 2018, the average invasion of dogs was reduced from 20.2 to 14.3%.

Diarrhetic Shellfish Poisoning: Evaluation of Enzyme-Linked Immunosorbent Assay Methods for Determination of Dinophyslstoxin-2

1995 ◽  
Vol 78 (6) ◽  
pp. 1403-1407 ◽  
Author(s):  
Eoin P Carmody ◽  
Kevin J James ◽  
Seán S Kelly
Keyword(s):  
Immunosorbent Assay ◽  
Cross Reactivity ◽  
Regulatory Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Shellfish Poisoning ◽  
Diarrhetic Shellfish Poisoning ◽  
Elisa Method ◽  
Multiple Samples ◽  
Liquid Chromatographic

Abstract Dinophysistoxin-2 (DTX-2), an isomer of okadaic acid (OA), recently has been found in Irish waters. DTX-2 was the predominant toxin during prolonged infestations in cultivated mussels along the southwest coast of Ireland. Substantial variations in toxin levels may exist both horizontally and vertically in the water column. The need to take multiple samples and the ethical concern about the use of mammals for routine quality control of shellfish prompted examination of 2 commercially available enzyme-linked immunosorbent assay (ELISA) methods, designed to detect OA, for determination of both OA and DTX-2. One ELISA method (DSPCheck, Sceti Co. Ltd., (Tokyo, Japan) showed good cross-reactivity (40 ± 5%) with standard DTX-2. This study showed that both ELISA methods show good correlation with the liquid chromatographic analysis of 9-anthryldiazomethane derivatives when OA is the predominant toxin present. The sensitivity was also good for OA determination using both methods, which allowed toxin measurement at 10 ng/mL (0.5 ng/well). This level is equivalent to 0.03 μg/g mussel meat. Blank mussel samples spiked with DTX-2 standards gave a good linear correlation (r = 0.997) with this ELISA method when toxin levels were 0.03-0.3 μg/g mussel meat. This range is appropriate for regulatory control of diarrhetic shellfish poisoning.

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