scholarly journals Geomicrobiología para prospección de uranio en profundidad en la cuenca Neuquina, Argentina

Tecnura ◽  
2021 ◽  
Vol 25 (69) ◽  
pp. 101-119
Author(s):  
Adalgisa Scotti ◽  
Guillermo Rojas ◽  
Cristina Marcela Servant ◽  
Pablo Catálfamo ◽  
Nicolás Valle ◽  
...  
Keyword(s):  
Bacillus Sp

Contexto: La prospección microbiológica de petróleo es una metodología ampliamente utilizada y se describe en muchos trabajos publicados, sin embargo en la minería de uranio (U) no es frecuente, si bien hay investigadores que se refierena algunos microorganismos como Bacillus sp, los cuales se utilizan en biorremediación y podrían ser capaces de hacerlo en prospección. En este trabajo se propone una relación microbiológica para la prospección por U, hipotetizando que se verá afectada por la presencia de U profundo Nuestro objetivo principal es mostrar que la relación microbiológica formadapor Bacillus sp /Mesófilos Aerobios Totales (B/TAM) se relaciona con la presencia de U y se puede utilizar como técnica complementaria para su prospección. Método: El muestreo de suelo fue realizado en un área de 600 Km2 en la cuenca Neuquina; Colonia Catriel, Provincia deRío Negro, Argentina. Para las determinaciónes de U espectrométrico, U total, U lábil y la microbiología, se muestrearon 62 sitios por duplicado y se estudiaron 8 pozos con diferente concentración de U en profundidad. Además, se analizaron la emanometría de Radón y las zoneografías de las concentraciones de U total, lábil y espectrométrico y de la relación micro-biológica. Los resultados se sometieron a un análisis estadístico mediante análisis de componentes principales y regresión lineal. Resultados: Este trabajo muestra una lineal correlación estadística altamente significativa (R= 0,59 p<0,002) entre la geomicrobiología en superficie y la concentración de U en profundidad. Conclusiones: Las investigaciones deben continuar vinculando otros elementos de la geoquímica y de la geofísica que podrían complementarse con esta técnica geomicrobiológica

МЕТАБОЛИТЫ ПРОБИОТИЧЕСКИХ БАКТЕРИЙ ОТВЕЧАЮТ ЗА ЭФФЕКТИВНОСТЬ ДЕЙСТВИЯ ПРОБИОТИКА "ПРОФОРТ®"

2019 ◽  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Broiler Chicks ◽  
Bacillus Sp

МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY

A primary study on nattokinase of bacterium Bacillus sp. isolated from nem chua

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Nguyen Quynh Uyen ◽  
Hoang Thu Ha ◽  
Nguyen Hong Nhung ◽  
Phan Thi Ha ◽  
Nguyen Huynh Minh Quyen
Keyword(s):  
Primary Study ◽  
Bacillus Sp ◽  
Bacterium Bacillus

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

Isolation and identification of lactic acid bacteria in Bakasang as fermented microbe starter

2013 ◽  
pp. 48
Author(s):  
J Aquarista Ingratubun ◽  
Frans G Ijong ◽  
Hens Onibala
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Fermented Food ◽  
Plate Count ◽  
Local Market ◽  
Bacillus Sp ◽  
Isolation And Identification ◽  
Total Plate Count

Food fermentation is one of various food processing techniques that has sufficient benefits of nutrition values, and also contains lactic acid bacteria which potentially inhibit pathogenic bacteria, thus prolong shelf life of  products. Bakasang is a traditional fermented food from North Sulawesi since many years ago. Reported research of bakasang previously had described that lactic acid bacteria was the dominant isolates and therefore current research  aimed to isolate and identify the lactic acid bacteria which associated during fermentation day 1 and day 15, respectively. Raw materials used were 5 kg intestine and liver of skipjack brought from local market Bersehati Manado. The intestine and liver of skipjack were washed and smashed and mixed with 10% salt  and 5% rice  from weight of the samples and then filled into bottle to be fermented for 15 days. Every 3 days (1,3,6,9,12,15), the samples were collected and analyzed for total lactic acid bacteria by using Total Plate Count Method on de Mann Rogosa Sharpe Agar after incubation at 37°C for 24 h. The colonies  grown were transferred to Tryptic Soy Broth and followed by streaking them on Tryptic Soy Agar and the free growing colony on agar medium were isolated into slant agar which were used for biochemical test such as Gram’s staining, motility test, catalase test, oksidase test, H2S test, IMVIC test (Indole, Methyl Red, Voges Proskauer, Citrate) and carbohydrate fermentation. The results showed that Lactobacillus sp., Bacillus sp., Eubacterium sp., and Bifidobacterium sp. All these four bacteria were distributed from day 1 to day 15 of the fermentation process© Fermentasi bahan pangan merupakan salah satu dari sekian banyak teknik pengolahan makanan yang mempunyai banyak manfaat dari kualitas gizi, mengandung bakteri asam laktat sehingga menghambat bakteri patogen sehingga daya simpan lebih panjang. Bakasang merupakan makanan fermentasi tradisional masyarakat Sulawesi Utara yang sudah ada sejak lama. Penelitian yang telah dilakukan terhadap bakasang menghasilkan informasi bahwa terdapat bakteri asam laktat pada bakasang sehingga menjadi tujuan untuk mengisolasi dan identifikasi bakteri asam laktat selama proses fermentasi 1-15 hari. Bahan baku bakasang ialah jeroan (usus dan hati) ikan cakalang Katsuwonis pelamis sebanyak 5 kg yang diambil dari pasar Bersehati Manado. Sampel jeroan dibersihkan kemudian dihancurkan, ditambahkan garam 10% dan nasi 5% kemudian difermentasi selama 15 hari dengan mengambil tiap-tiap sampel setiap 1, 3, 6, 9, 12, dan 15 untuk dihitung jumlah bakteri asam laktat dengan menggunakkan metode Total Plate Count pada media de Mann Rogosa Sharpe Agar dan koloni yang tumbuh di tumbuhkan  kembali pada media Tryptic Soy Broth  dan digores kembali pada media Tryptic Soy Agar, koloni yang tumbuh digores pada media slant agar yang selanjutnya diidentifikasi bakteri asam laktat berdasarkan uji biokimia yaitu uji pewarnaan Gram, uji motility, uji katalase, uji oksidase, uji H2S dan uji IMVIC (Indole, MethylRed, Voges Proskauer, Citrate). Hasil menunjukkan bahwa selama proses fermentasi berlangsung terdapat 4 genera bakteri asam laktat sesuai yaitu Lactobacillus sp., Bacillus sp., Eubacterium sp., dan Bifidobacterium sp., ke 4 genera ini tersebar pada fermentasi hari 1 sampai hari ke 15©

Isolation and identification of a flocculant-producing Bacillus sp. and analysis of the properties of the bioflocculant

2013 ◽  
Vol 19 (4) ◽  
pp. 647-653
Author(s):  
Weiwei ZHAO ◽  
Xiuhua WANG ◽  
Zhen SUN ◽  
Jie HUANG ◽  
Yansong ZHU ◽  
...  
Keyword(s):  
Bacillus Sp ◽  
Isolation And Identification

Isolation, screening and optimization of amylase producing Bacillus sp. from soil

2015 ◽  
Vol 2 (3) ◽  
pp. 86-93 ◽  
Author(s):  
V. Singh ◽  
Richa Sharma ◽  
Poonam Sharma
Keyword(s):  
Bacillus Sp

Isolation, identification and in silico study of native cellulase producing bacteria

2019 ◽  
Vol 17 ◽  
Author(s):  
Farzane Kargar ◽  
Mojtaba Mortazavi ◽  
Mahmood Maleki ◽  
Masoud Torkzadeh Mahani ◽  
Younes Ghasemi ◽  
...  
Keyword(s):  
Growth Rate ◽  
Bacillus Cereus ◽  
16S Rdna ◽  
Enzyme Production ◽  
Rational Design ◽  
Cellulase Production ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Chain Polymer ◽  
Bacillus Toyonensis

Aims: The purpose of this study was to screen the bacteria producing cellulase enzymes and their bioinformatics studies. Background: Cellulose is a long-chain polymer of glucose that hydrolyzes by cellulases to glucose molecules. In order to design the new biotechnological applications, some strategies have been used as increasing the efficiency of enzyme production, generating cost-effective enzymes, producing stable enzymes and identification of new strains. Objective: On the other hand, some bacteria special features have made them suitable candidates for the identification of the new source of enzymes. In this regard, some native strains of bacteria were screened. Method: These bacteria were grown on a culture containing the liquid M9 media containing CMC to ensure the synthesis of cellulase. The formation of a clear area in the culture medium indicated decomposition of cellulose. In the following, the DNA of these bacteria were extracted and their 16S rDNA genes were amplified. Result: The results show that nine samples were able to synthesize cellulase. In following, these strains were identified using 16S rDNA. The results show that these screened bacteria belonged to the Bacillus sp., Alcaligenes sp., Alcaligenes sp., and Enterobacter sp.conclusionThe enzyme activity analysis shows that the Bacillus toyonensis, Bacillus sp. strain XA15-411 Bacillus cereus have produced the maximum yield of cellulases. However, these amounts of enzyme production in these samples are not proportional to their growth rate. As the bacterial growth chart within 4 consecutive days shows that the Alcaligenes sp. Bacillus cereus, Bacillus toyonensis, Bacillus sp. strain XA15-411 have a maximum growth rate. The study of the phylogenetic tree also shows that Bacillus species are more abundant in the production of cellulase enzyme. These bioinformatics analyses show that the Bacillus species have different evolutionary relationships and evolved in different evolutionary time. Other: However, for maximum cellulase production by this bacteria, some information as optimum temperature, optimum pH, carbon and nitrogen sources are needed for the ideal formulation of media composition. The cellulase production is closely controlled in microorganisms and the cellulase yields appear to depend on a variety of factors. However, the further studies are needed for cloning, purification and application of these new microbial cellulases in the different commercial fields as in food, detergent, and pharmaceutical, paper, textile industries and also various chemical industries. However, these novel enzymes can be further engineered through rational design or using random mutagenesis techniques.

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