scholarly journals Diversity of Rhizoctonia solani Isolates of Rice Varieties of Ciherang, IR 64, Mekongga, and Situ Bagendit

2021 ◽  
Vol 17 (4) ◽  
pp. 141-150
Author(s):  
Arina Manasikana ◽  
Sri Sulandari ◽  
Achmadi Priyatmojo
Keyword(s):  
Oryza Sativa ◽  
Rhizoctonia Solani ◽  
Rice Varieties

Padi (Oryza sativa) termasuk ke dalam komoditas penting di Indonesia. Salah satu penyakit penting pada tanaman padi ialah penyakit hawar pelepah yang disebabkan oleh Rhizoctonia solani. Tujuan penelitian ini untuk mengetahui karakteristik dan kelompok anastomosis R. solani yang diisolasi dari tanaman padi varietas Ciherang, IR 64, Mekongga, dan Situ Bagendit; dan mengetahui keragaman genetiknya menggunakan primer universal, dan tingkat kekerabatannya. Penelitian dilaksanakan pada Oktober 2019 hingga Juli 2020 di Laboratorium Teknologi Pengendalian UGM. Pengambilan sampel dilaksanakan di Kecamatan Pandak, Bantul yang selanjutnya dilakukan isolasi dan pemurnian cendawan Rhizoctonia menggunakan medium agar-agar dekstrosa kentang. Isolat R. solani diklasifikasikan berdasarkan keragaman kultur, keragaman morfologi, jumlah inti sel, kemampuan anastomosis (AG), dan keragaman genetik. Analisis keragaman genetik dilakukan dengan PCR menggunakan primer universal ITS1 dan ITS4. Berdasarkan pengamatan keragaman kultur dan keragaman morfologi diperoleh hasil yang bervariasi. Hasil pengamatan jumlah inti sel pada keseluruhan isolat berkisar antara 5 hingga 7 inti pada sel yang termasuk dalam kategori multinukleat. Pengamatan kelompok anastomosis (AG) pada 13 isolat yang digunakan masuk ke dalam kategori C3 (anastomosis sempurna). Analisis PCR diperoleh pita DNA dengan hasil sesuai target yaitu 600–750 pb. Hasil secara sikuensing diketahui bahwa 12 isolat R. solani menunjukkan kekerabatan yang tinggi dengan isolat AG-1 IA, kecuali pada isolat CH 3.

scholarly journals Berlian, SOJ A3 and Jawa Local Rice Varieties Phylogenetically Distinct From Oryza sativa Indica and Oryza sativa Japonica

el–Hayah ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 112-125
Author(s):  
Yudrik Lathif ◽  
Riri Wiyanti Retnaningtyas ◽  
Dwi Listyorini ◽  
Suharti Suharti
Keyword(s):  
Oryza Sativa ◽  
Phylogenetic Tree ◽  
Dna Barcode ◽  
Rbcl Gene ◽  
Tree Reconstruction ◽  
Rice Varieties ◽  
Local Varieties ◽  
Precise Position ◽  
Total Dna ◽  
Taxonomical Position

The genetic resources identification of Indonesian local rice varieties is a crucial work should be done to conserve our native germplasm. This research aimed to know the taxonomical position of East Java local rice varieties including Jawa (JW), Berlian (BR), and SOJ A3 (SJ) using DNA barcode based on rbcL gene. Total DNA of each sample was isolated from leaves. A pair of forward 5'-ATG TCA CCA CAA ACA SJA AC-3' and reverse 5'-TCG GTA CCT GCA GTA GC-3' primers were used to amplify fragments of rbcL gene resulting in 751bp, 755bp, and 754bp fragments from BR, SJ, and JW varieties, respectively. Phylogenetic tree reconstruction revealed that our three local varieties were forming a cluster separated from the widely cultivated subspecies Oryza sativa Indica and Oryza sativa Japonica. However, further studies are necessary to reveal a more precise position of the local varieties in a phylogenetic tree on the species level.

Breeding for Improving Human Nutrition and Grain Quality through Biofortification of Traditional Land Races and Improved Cultivars in Rice (Oryza sativa L.)

2017 ◽  
pp. 126-148
Author(s):  
P. Savitha ◽  
R. Usha Kumari
Keyword(s):  
Oryza Sativa ◽  
Tamil Nadu ◽  
Oryza Sativa L ◽  
Farming Systems ◽  
Total Production ◽  
Micronutrient Deficiencies ◽  
Rice Varieties ◽  
The Family ◽  
Iron And Zinc

Rice (Oryza sativa L.) which belongs to the family poaceae, is the life and the prince among cereals as this unique grain helps to sustain two thirds of the world's population. India have traditionally been used or rice cultivation due to availability of water and conductive soil (Nadesa panic). Rice was grown in 155.30 million hectares and around 600 million tonnes of rice harvested each year globally. About 114 countries produce rice with China and India supporting 50 per cent of total production. India stands first in area under cultivation (41.85 mha) and ranks second in production, where as China ranks first in production with just half of the area. India's production is 89.13 million tonnes with a productivity of 2130 kg/ha. Among the rice producing states of India, Tamil Nadu ranks sixth in production (6.53 million tonnes) and second in productivity of 3918 kg/ha with an area of 20.16 lakh ha (Season and Crop Report, 2012). Local farmers in such farming systems retain the traditional varieties based on their knowledge of the quality of the rice varieties and their adaptation to varying agro-ecological situations created by diverse seasons and land situations (Tapasi das & Ashesh Kumar Das, 2014). Among the major micronutrient deficiencies common in rice consuming countries, iron and zinc deficiencies (“hidden hunger”), affect over three billion people worldwide, mostly in developing countries (Welch & Graham, 2004).

scholarly journals Susceptibility of six varieties of rice to the infestation of rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

2012 ◽  
Vol 21 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Humayun Reza Khan ◽  
Polash Kanti Halder
Keyword(s):  
Oryza Sativa ◽  
Emerging Adults ◽  
Sitophilus Oryzae ◽  
Ambient Condition ◽  
Rice Weevil ◽  
Rice Varieties ◽  
Moisture Contents ◽  
The Mean

The susceptibility of six varieties of rice, Oryza sativa, viz. Lata, Minicate, Nazersail, Parija, Kalijira and Kataribhog, to the infestation of the rice weevil, Sitophilus oryzae (L.), was studied on the basis of population build up under an ambient condition (28 ± 4ºC and 70 ± 4 % RH) of the laboratory. The mean weights of each grain of the above six rice varieties were 16.9, 16.1, 11.43, 13.87, 6.23 and 9.77 mg, respectively; mean lengths were 6.47, 6.56, 5.37, 5.38, 4.22 and 4.92 mm, respectively; mean widths were 2.45, 2.03, 2.05, 2.17, 1.6 and 1.9 mm, respectively; and moisture contents were 11.55, 10.75, 11.6, 12.71, 11.85, and 12.1 per cents, respectively. As far as the number of emerging adults is concerned, the weevil showed the highest number (695) in Nazersail on the 16th week, Lata (755) on the 18th week, Minicate (654) on the 16th week, Parija (482) on the 20th week, Kalijira (402) on the 20th week, and Kataribhog (456) on the 20th week. The mean numbers of the adult weevils in the above six varieties after 22 weeks of rearing were 425, 410, 351, 387, 357 and 400, respectively and was statistically significant at 5% level. The degree of susceptibility of the rice varieties to the rice weevils from the highest to lowest susceptibility was ? Lata > Nazersail > Minicate > Pariza > Kataribhog > Kalijira.DOI: http://dx.doi.org/10.3329/dujbs.v21i2.11514 Dhaka Univ. J. Biol. Sci. 21(2): 163-168, 2012 (July)

Proline and carbohydrate metabolism in rice varieties (Oryza sativa L.) under various drought and recovery conditions

2019 ◽  
Vol 24 (3) ◽  
pp. 376-387 ◽  
Author(s):  
Doan Cong Dien ◽  
Thieu Thi Phong Thu ◽  
Kyi Moe ◽  
Takeo Yamakawa
Keyword(s):  
Oryza Sativa ◽  
Carbohydrate Metabolism ◽  
Oryza Sativa L ◽  
Rice Varieties

Screening and comprehensive evaluation of rice (Oryza sativa L. subsp. japonica Kato) germplasm resources for nitrogen efficiency in Xinjiang, China

2020 ◽  
Vol 18 (3) ◽  
pp. 179-189
Author(s):  
Chunping Jia ◽  
Fengbin Wang ◽  
Jie Yuan ◽  
Yanhong Zhang ◽  
Zhiqiang Zhao ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Relative Yield ◽  
N Fertilizer ◽  
Nitrogen Efficiency ◽  
Environment Interaction ◽  
Germplasm Resources ◽  
Rice Varieties ◽  
N Application ◽  
N Efficiency ◽  
Panicle Number

AbstractComprehensive screening of rice (Oryza sativa L. subsp. japonica Kato) germplasm resources with different nitrogen (N) efficiency levels is effective for improving N use efficiency (NUE) while reducing pollution and providing high quality, yield, and efficiency agriculture. We investigated 14 indices of 38 varieties under three N application levels to assess differences among genotypes. Rice varieties were classified for screening and identifying N efficient. Descriptive statistical analysis results indicated significant differences in relative yield, and also in NUE indices (agronomic utilization rate and partial productivity of N fertilizer). The genotype main effects and genotype–environment interaction effects (GGE) biplot analysis was used to evaluate suitable varieties, compare the stable and high yield capabilities of different varieties, find the ideal variety, and describe the correlation, discrimination and representativeness of the indices under different N application levels. Descriptive statistical, discrimitiveness and representativeness and factor analysis were used to select indices, in which the panicle number per plant and soil and plant analyzer development (SPAD) value were the key indices for evaluation and identification. Heatmap and hierarchical cluster analysis based on the average value of evaluation indices, and scatter plot based on the comprehensive value of N efficiency (P) according to formula showed that all varieties could be divided into five types under different N treatments. Our findings work toward developing N efficient rice varieties to improve NUE, reduce N fertilizer application and thus N waste, consequently mitigating the effects of rice production on the environment to ensure food security and sustainable agricultural development.

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