Alkaline Pretreatment of Sweet Sorghum Bagasse for Bioethanol Production

To improve the efficiency of bioethanol production, an advanced process was required to extract ethanol from solid-state fermented feedstock. With regard to the characteristics of no fluidity of solid biomass, a continuous solid-state distillation (CSSD) column was designed with a proprietary rotary baffle structure and discharging system. To optimize the operation condition, fermented sweet sorghum bagasse was prepared as feedstock for a batch distillation experiment. The whole distillation time was divided into heating and extracting period which was influenced by loading height and steam flow rate simultaneously. A total of 16 experiments at four loading height and four steam flow rate levels were conducted, respectively. Referring to packing, rectifying column, mass, and heat transfer models of the solid-state distillation heating process were established on the basis of analyzing the size distribution of sweet sorghum bagasse. The specific heat capacity and thermal conductivity value of fermented sweet sorghum bagasse were tested and served to calculate the ethanol yielding point and concentration distribution in the packing. The extracting process is described as the ethanol desorption from porous media absorbent and the pseudo-first-order desorption dynamic model was verified by an experiment. Benefit (profit/time) was applied as objective function and solved by successive quadratic programming. The optimal solution of 398 mm loading height and 8.47 m3/h steam flow rate were obtained to guide a 4 m in diameter column design. One heating and two extracting trays with 400 mm effective height were stacked up in an industrial CSSD column. The steam mass flow rate of 0.5 t/h was determined in each tray and further optimized to half the amount on the third tray based on desorption equation.

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Design and performance of amphiphilic lignin derivatives in enzymatic hydrolysis of sweet sorghum bagasse for bioethanol production

BioResources ◽

10.15376/biores.16.3.5875-5889 ◽

2021 ◽

Vol 16 (3) ◽

pp. 5875-5889

Author(s):

Nissa Nurfajrin Solihat ◽

Rika Raniya ◽

Triyani Fajriutami ◽

Apri Heri Iswanto ◽

Widya Fatriasari ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Sweet Sorghum ◽

Cellulose Content ◽

Sweet Sorghum Bagasse ◽

Bioethanol Production ◽

Hydrolysis Time ◽

Peg 4000 ◽

Sorghum Bagasse ◽

The Impact ◽

Hydrolysis Of

Sweet sorghum bagasse (SSB) is potential feedstock for bioethanol production due to its natural abundance and high cellulose content (> 40%). This work compared the impact of three variables relative to the enzymatic hydrolysis of SSB kraft pulp. The three variables were the biosurfactant from lignin derivative known as amphiphilic lignin derivatives (A-LD), the enzyme loading level, and the hydrolysis time. These variables were optimized by response surface methodology (RSM) with a Box-Behnken design (BBD). The concentration of polyethylene glycol (PEG) 4000 was also optimized to compare it with the A-LD performance in the enzymatic hydrolysis process. After optimization, the A-LD produced a higher reducing sugar yield (RSY) (99.45%) than the PEG 4000. The difference in the predicted versus experimental values of the RSY was less than 4%, which means that the model was highly predictive. The adequacy of the model was confirmed by a regression value close to 1 for the A-LD assisted test. The result implies that the A-LD significantly improved the enzymatic hydrolysis performance to enhance the RSY. Moreover, the BBD is adequate and useful to identify the optimum concentration of surfactant.

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Lignin relocation contributed to the alkaline pretreatment efficiency of sweet sorghum bagasse

Fuel ◽

10.1016/j.fuel.2015.05.029 ◽

2015 ◽

Vol 158 ◽

pp. 152-158 ◽

Cited By ~ 48

Author(s):

Zhipei Yan ◽

Jihong Li ◽

Sandra Chang ◽

Ting Cui ◽

Yan Jiang ◽

...

Keyword(s):

Sweet Sorghum ◽

Alkaline Pretreatment ◽

Sweet Sorghum Bagasse ◽

Sorghum Bagasse

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Bioprocess engineering of bioethanol production based on sweet sorghum bagasse by co-culture technique using Trichodermareesei and Saccharomyces cerevisiae

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/209/1/012018 ◽

2018 ◽

Vol 209 ◽

pp. 012018

Author(s):

E A Syadiah ◽

L Haditjaroko ◽

K Syamsu

Keyword(s):

Saccharomyces Cerevisiae ◽

Sweet Sorghum ◽

Culture Technique ◽

Sweet Sorghum Bagasse ◽

Bioethanol Production ◽

Bioprocess Engineering ◽

Sorghum Bagasse

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The Effects of Alkaline Pretreatment on Agricultural Biomasses (Corn Cob and Sweet Sorghum Bagasse) and Their Hydrolysis by a Termite-Derived Enzyme Cocktail

Agronomy ◽

10.3390/agronomy10081211 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1211 ◽

Cited By ~ 1

Author(s):

Mpho. S. Mafa ◽

Samkelo Malgas ◽

Abhishek Bhattacharya ◽

Konanani Rashamuse ◽

Brett I. Pletschke

Keyword(s):

Sweet Sorghum ◽

Compositional Analysis ◽

Value Added ◽

Alkaline Pretreatment ◽

Sweet Sorghum Bagasse ◽

Amorphous Cellulose ◽

Naoh Pretreatment ◽

Enzyme Cocktail ◽

Sorghum Bagasse ◽

Multifunctional Enzymes

Sweet sorghum bagasse (SSB) and corncob (CC) have been identified as promising feedstocks for the production of second-generation biofuels and other value-added chemicals. In this study, lime (Ca(OH)2) and NaOH pretreatment efficacy for decreasing recalcitrance from SSB and CC was investigated, and subsequently, the pretreated biomass was subjected to the hydrolytic action of an in-house formulated holocellulolytic enzyme cocktail (HEC-H). Compositional analysis revealed that SSB contained 29.34% lignin, 17.75% cellulose and 16.28% hemicellulose, while CC consisted of 22.51% lignin, 23.58% cellulose and 33.34% hemicellulose. Alkaline pretreatment was more effective in pretreating CC biomass compared to the SSB biomass. Both Ca(OH)2 and NaOH pretreatment removed lignin from the CC biomass, while only NaOH removed lignin from the SSB biomass. Biomass compositional analysis revealed that these agricultural feedstocks differed in their chemical composition because the CC biomass contained mainly hemicellulose (33–35%), while SSB biomass consisted mainly of cellulose (17–24%). The alkaline pretreated SSB and CC samples were subjected to the hydrolytic action of the holocellulolytic enzyme cocktail, formulated with termite derived multifunctional enzymes (referred to as MFE-5E, MFE-5H and MFE-45) and exoglucanase (Exg-D). The HEC-H hydrolysed NaOH pretreated SSB and CC more effectively than Ca(OH)2 pretreated feedstocks, revealing that NaOH was a more effective pretreatment. In conclusion, the HEC-H cocktail efficiently hydrolysed alkaline pretreated agricultural feedstocks, particularly those which are hemicellulose- and amorphous cellulose-rich, such as CC, making it attractive for use in the bioconversion process in the biorefinery industry.

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